Análisis genético de la vía Erk-Mapk en el desarrollo y mantenimiento de tumores de pulmón promovidos por K-Ras G12V

Tesis doctoral inédita realizada en el Departamento de Bioquímica de la Facultad de Medicina de la Universidad Autónoma de Madrid y el Centro Nacional de Investigaciones Oncológicas (CNIO). Fecha de lectura: 3 de Noviembre de 201

Caracterización Molecular de la Diversidad Fúngica de los Bosques Llucud y Palictahua: Potencialidades en Control Biológico/Molecular Characterization of Diversity Fungic of the Llucud and Palictahua Forests: Potential in Biological Control

Dentro de los microorganismos más abundantes que se pueden encontrar en formaciones ecosistémicas naturales como los bosques andinos del Ecuador se encuentran los hongos microscópicos, los cuales desempeñan funciones cruciales en dichos ecosistemas. Por lo cual el objetivo de esta investigación fue caracterizar molecularmente la diversidad de hongos presentes en los bosques nativos Llucud y Palictahua, estableciendo sus potencialidades de uso en el control biológico de plagas y enfermedades que afectan a los cultivos agrícolas y cuyo control en su mayoría se lo realiza con plaguicidas químicos. Mediante secuenciación de próxima generación (NGS por sus siglas en inglés) de las muestras compuestas de suelo tomadas del horizonte “A” (rizósfera) de cada bosque, se identificaron 56 especies de hongos en Palictahua y 38 en Llucud, presentándose en ambos bosques un total de 6 hongos con importantes potencialidades para su uso en el control biológico, dentro de las cuales se encontraron: Brachyphoris oviparasitica (nematófago), Simplicillium (entomopatógeno y micoparásito), Hamamotoa lignophila (levadura con actividad Killer) en Llucud, y Metarhizium robertsii (entomopatógeno), Brachyphoris oviparasitica (nematófago) y Paraphaeosphaeria parmeliae (micoparásito) en Palictahua. El Bosque Palictahua presentó mayor diversidad de hongos que el bosque Llucud, sin embargo es importante cuidar ambos bosques, pues poseen una gran riqueza microbiana con un sinnúmero de posibilidades de uso en la medicina, industria, biotecnología y otros campos. Among the most abundant microorganisms that can be found in natural ecosystem formations such as the Andean forests of Ecuador are microscopic fungi, which play crucial roles in these ecosystems. So that the objective of this research was to molecularly characterize the diversity of fungi present in the native forests Llucud and Palictahua, establishing their potential for use in the biological control of pests and diseases that affect agricultural crops and whose control is mostly carried out with chemical pesticides. Through next-generation sequencing (NGS for its acronym in English) of compound samples of soil took from “A” horizon (rhizosphere). 56 species of fungi were identified in Palictahua and 38 in Llucud, presenting in both forests a total of 6 fungi with significant potential for use in biological control, among which were found: Brachyphoris oviparasitica (nematophagous), Simplicillium sp. (entomopathogen and mycoparasite), Hamamotoa lignophila (yeast with Killer activity) in Llucud, and Metarhizium robertsii (entomopathogen), Brachyphoris oviparasitica (nematophagous) and Paraphaeosphaeria parmeliae (mycoparasite) in Palictahua. The Palictahua forest presented greater diversity of fungi than the Llucud forest, however it is important to take care of both forests, since they have a great microbial richness with a myriad of possibilities of use in medicine, industry, biotechnology and other fields. Palabras clave: Biodiversidad, Microbiota, Plaguicidas. Keywords: Biodiversity, Microbiota, Pesticides

Ajitts: adaptive just-in-time transaction scheduling

Lecture Notes in Computer Science 7891, 2013Distributed transaction processing has benefited greatly from optimistic concurrency control protocols thus avoiding costly fine-grained synchronization. However, the performance of these protocols degrades significantly when the workload increases, namely, by leading to a substantial amount of aborted transactions due to concurrency conflicts. Our approach stems from the observation that when the abort rate increases with the load as already executed transactions queue for longer periods of time waiting for their turn to be certified and committed. We thus propose an adaptive algorithm for judiciously scheduling transactions to minimize the time during which these are vulnerable to being aborted by concurrent transactions, thereby reducing the overall abort rate. We do so by throttling transaction execution using an adaptive mechanism based on the locally known state of globally executing transactions, that includes out-of-order execution. Our evaluation using traces from the industry standard TPC-E workload shows that the amount of aborted transactions can be kept bounded as system load increases, while at the same time fully utilizing system resources and thus scaling transaction processing throughput.(undefined

Semi-inclusive charged-current neutrino-nucleus cross sections in the relativistic plane-wave impulse approximation

Neutrino-nucleus quasielastic scattering is studied in the plane-wave impulse approximation for three nuclear models: the relativistic Fermi gas (RFG), the independent-particle shell model (IPSM), and the natural orbitals (NO) model with Lorentzian dependence of the excitation energy. A complete study of the kinematics of the semi-inclusive process and the associated cross sections are presented and discussed for 40Ar and 12C. Inclusive cross sections are also obtained by integrating the semi-inclusive expressions over the outgoing hadron. Results are consistent with previous studies restricted to the inclusive channel. In particular, a comparison with the analytical results for the RFG model is performed. Explicit expressions for the hadronic tensor and the 10 semiinclusive nuclear responses are given. Theoretical predictions are compared with semi-inclusive experimental data from T2K experiment.Ministerio de Ciencia, Innovación y Universidades de España y FEDER (Fondo Europeo de Desarrollo Regional). FIS2017-88410-PJunta de Andalucía. FQM160 y SOMM17/6105/UG

Standardization of hake LPUE series of the Galician set-longline fleet in Subarea VII

WGHMM (now WGBIE) identified a problem in the assessment of northern hake in relation to the scarce information on the abundance of large fish. 2004 WKSOUTH tested the inclusion in SS3 of Galician LPUEs from set-longline fleet targeting hake in ICES Subarea VII. This metier catches mainly adults. However, during WGBIE 2014, a serious inconsistency was detected when updating this LPUE time series, related to the assumption of the average fishing days by trip employed along the time series. The current working document provides the revision of this LPUE series by applying the actual number of fishing days by trip recorded in logbooks, which has varied greatly in the final part of the time series. The revised LPUE indices obtained were then tested in the assessment of northern hake stock. The difference in results between the assessments without LLPUE and the assessment which includes the new LPUE series were minor. In the initial part of the time series the LPUE matched the abundance closely but in the last period the increase in the LLPUE was much lower than the increase in the stock abundance

Semi-inclusive charged-current neutrino-nucleus reactions: Analysis of data in the Relativistic Plane-Wave Impulse Approximation

Semi-inclusive neutrino-nucleus cross sections within the plane-wave impulse approximation (PWIA) for three nuclear models: relativistic Fermi gas (RFG), independent-particle shell model (IPSM) and natural orbitals shell model (NO) are compared with the available CC0π measurements from the T2K, MINERνA and MicroBooNE collaborations where a muon and at least one proton were detected in the final state. Results are presented as a function of the momenta and angles of the final particles, as well as in terms of the imbalances between proton and muon kinematics. The analysis reveals that contributions beyond PWIA are crucial to explain the experimental measurements and that the study of correlations between final-state proton and muon kinematics can provide valuable information on relevant nuclear effects such as initial state dynamics and final state interactions.Ministerio de Ciencia, Innovación y Universidades FIS2017-88410-PJunta de Andalucía PID2020-114687GB-10

Looking for blazars in a sample of unidentified high-energy emitting Fermi sources

Context. Based on their overwhelming dominance among associated Fermi γ-ray catalogue sources, it is expected that a large fraction of the unidentified Fermi objects are blazars. Through crossmatching between the positions of unidentified γ-ray sources from the First Fermi Catalog of γ-ray sources emitting above 10 GeV (1FHL) and the ROSAT and Swift/XRT catalogues of X-ray objects and between pointed XRT observations, a sample of 36 potential associations was found in previous works with less than 15 arcsec of positional offset. One-third of them have recently been classified; the remainder, though believed to belong to the blazar class, still lack spectroscopic classifications. Aims. We study the optical spectrum of the putative counterparts of these unidentified gamma-ray sources in order to find their redshifts and to determine their nature and main spectral characteristics. Methods. An observational campaign was carried out on the putative counterparts of 13 1FHL sources using medium-resolution optical spectroscopy from the Osservatorio Astronomico di Bologna in Loiano, Italy; the Telescopio Nazionale Galileo and the Nordic Optical Telescope, both in the Canary Islands, Spain; and the Observatorio Astronómico Nacional San Pedro Mártir in Baja California, Mexico. Results. We were able to classify 14 new objects based on their continuum shapes and spectral features. Conclusions. Twelve new blazars were found, along with one new quasar and one new narrow line Seyfert 1 (NLS1) to be potentially associated with the 1FHL sources of our sample. Redshifts or lower limits were obtained when possible alongside central black hole mass and luminosity estimates for the NLS1 and the quasar.Fil: Marchesini, Ezequiel Joaquín. Universidad Nacional de la Plata. Facultad de Ciencias Astronómicas y Geofísicas; ArgentinaFil: Masetti, Nicola. Istituto di Astrofisica Spaziale e Fisica Cosmica di Bologna; ItaliaFil: Chavushyan, V.. Instituto Nacional de Astrofísica, Óptica y Electrónica; MéxicoFil: Cellone, Sergio Aldo. Universidad Nacional de la Plata. Facultad de Ciencias Astronómicas y Geofísicas; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Astrofísica La Plata. Universidad Nacional de La Plata. Facultad de Ciencias Astronómicas y Geofísicas. Instituto de Astrofísica La Plata; ArgentinaFil: Andruchow, Ileana. Universidad Nacional de la Plata. Facultad de Ciencias Astronómicas y Geofísicas; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Astrofísica La Plata. Universidad Nacional de La Plata. Facultad de Ciencias Astronómicas y Geofísicas. Instituto de Astrofísica La Plata; ArgentinaFil: Bassani, L.. Istituto di Astrofisica Spaziale e Fisica Cosmica di Bologna; ItaliaFil: Bazzano, A.. Istituto di Astrofisica e Planetologia Spaziali; ItaliaFil: Jiménez-Bailón, E.. Universidad Nacional Autónoma de México; MéxicoFil: Landi, R.. Istituto di Astrofisica Spaziale e Fisica Cosmica di Bologna; ItaliaFil: Malizia, A.. Istituto di Astrofisica Spaziale e Fisica Cosmica di Bologna; ItaliaFil: Palazzi, E.. Istituto di Astrofisica Spaziale e Fisica Cosmica di Bologna; ItaliaFil: Patiño Álvarez, V.. Instituto Nacional de Astrofísica, Óptica y Electrónica; MéxicoFil: Rodríguez Castillo, G. A.. Osservatorio Astronomico di Roma; ItaliaFil: Stephen, J. B.. Istituto di Astrofisica Spaziale e Fisica Cosmica di Bologna; ItaliaFil: Ubertini, P.. Istituto di Astrofisica e Planetologia Spaziali; Itali

A New Porphyrin for the Preparation of Functionalized Water-Soluble Gold Nanoparticles with Low Intrinsic Toxicity

Altres ajuts: T.P. thanks the Generalitat de Catalunya for the AGAUR FI-DGR 2011 predoctoral grant.A potential new photosensitizer based on a dissymmetric porphyrin derivative bearing a thiol group was synthesized. 5-[4-(11-Mercaptoundecyloxy)-phenyl-10,15,20-triphenylporphyrin (PR-SH) was used to functionalize gold nanoparticles in order to obtain a potential drug delivery system. Water-soluble multifunctional gold nanoparticles GNP-PR/PEG were prepared using the Brust-Schiffrin methodology, by immobilization of both a thiolated polyethylene glycol (PEG) and the porphyrin thiol compound (PR-SH). The nanoparticles were fully characterized by transmission electron microscopy and 1H nuclear magnetic resonance spectroscopy, UV/Vis absorption spectroscopy, and X-ray photoelectron spectroscopy. Furthermore, the ability of GNP-PR/PEGs to induce singlet oxygen production was analyzed to demonstrate the activity of the photosensitizer. Cytotoxicity experiments showed the nanoparticles are nontoxic. Finally, cellular uptake experiments demonstrated that the functionalized gold nanoparticles are internalized. Therefore, this colloid can be considered to be a novel nanosystem that could potentially be suitable as an intracellular drug delivery system of photosensitizers for photodynamic therapy

Superscaling in the resonance region for neutrino-nucleus scattering: The SuSAv2 dynamical coupled-channels model

In this work, the SuSAv2 and dynamical coupled-channels (DCC) models have been combined and tested in the inelastic regime for electron and neutrino reactions on nuclei. The DCC model, an approach to study baryon resonances through electron and neutrino induced meson production reactions, has been implemented for the first time in the SuSAv2-inelastic model to analyze the resonance region. Within this framework, we also present a novel description about other inelasticities in the resonance region (SoftDIS). The outcomes of these approaches are firstly benchmarked against (e, e′) data on 12C. The description is thus extended to the study of neutrino-nucleus inclusive cross sections on 12C and 40Ar and compared with data from the T2K, MicroBooNE, ArgoNEUT, and MINERvA experiments, thus covering a wide kinematical range.Ministerio de Ciencia, Innovación y Universidades (MICINN). España PID2020–114687 GB-100European Commission (EC). Fondo Europeo de Desarrollo Regional (FEDER) PID2020–114687 GB-100Junta de Andalucía. FQM160, SOMM17/6105/UGR/UGR, P20-01247University of Tokyo. 2022i-J-001, 2023i-J-001INFN. Project Iniziativa Specifica NucSysUniversity of Turin.BARM-RILO-20Universidad de Sevilla. PID2020–114687 GB-10