A parasitic myoma: clinicopathological study of a rare case

Leiomyoma is a benign tumour composed of smooth muscle cells with fibrous stroma and it is the commonest tumour amongst the tumours of uterus. As per FIGO classification system parasitic leiomyoma has no myometrial involvement or uterine attachment. These myomas get detached from the uterus and receive the blood supply from another source. The etiology and pathologic basis of these parasitic fibroids is not yet clearly understood. We report a rare case of parasitic myoma in a 29 years old female patient presented with abdominal discomfort and difficulty in micturition. Clinical examination and subsequent imaging studies revealed a pelvic mass. Histopathological examination of which proved it to be a parasitic myoma. Parasitic myoma is a rare entity which may be iatrogenically created after surgery particularly with morcellation technique. With increasing rates of laparoscopic procedures, surgeons should be aware of the possibility of formation of parasitic myoma and should take intraoperative precautions to minimise its formation

Cytokines and inflammatory mediators: 25. Certolizumab Pegol has a Different Profile from the other Anti-TNFS, Including Golimumab, in a Variety of in Vitro Assays

Background: Activities of the anti-TNFs, certolizumab pegol (CZP), etanercept (ETA), infliximab (IFX) and adalimumab (ADA), have been compared in a range of in vitro assays. CZP is the only licensed PEGylated Fab' anti-TNF; ETA is a fusion protein with an IgG1 Fc, and IFX and ADA are both antibodies with an IgG1 Fc. Golimumab (GLM) is a monoclonal IgG1 TNF inhibitor recently approved for a number of indications; it is thus of interest to assess the in vitro activity of GLM. In vitro assays previously used were neutralisation of TNF in the L929 bioassay, inhibition of LPS-driven cytokine production by monocytes, induction of apoptosis in activated lymphocytes and monocytes, and induction of neutrophil necrosis. Methods: Neutralisation of human TNF was assessed in the L929 bioassay using a range of concentrations of the anti-TNFs and a fixed concentration of TNF (100 pg/mL). Activity of the anti-TNFs at inhibiting LPS-driven IL-1β secretion by monocytes was assessed by incubating peripheral blood monocytes with various concentrations of the anti-TNF for 1 hour (hr) and then washing the cells. LPS was added for 4 hrs, the supernatants collected and the IL-1β level measured by ELISA. To assess induction of apoptosis, peripheral blood lymphocytes were activated for 2 days with 2 μg/mL CD3/CD28 and monocytes with 300 U/mL IL-4 and GMCSF for 3 days. The effect of the anti-TNFs on apoptosis was assessed by Annexin V staining using flow cytometry 24 hrs later. The effect of the anti-TNFs on neutrophil necrosis was determined by measuring myeloperoxidase release after 12 hrs. An isotype-matched control was used in all assays except the L929 bioassay. Results: IC90 neutralisation activity of the anti-TNFs in the L929 bioassay was 0.3 ng/mL for ETA, 4 ng/mL for GLM, 15 ng/mL for ADA, and 20 ng/mL for IFX, compared with 2.5 ng/mL for CZP. CZP was the most potent inhibitor of LPS-driven IL-1β secretion (IC50 ∼0.1 ng/mL), followed by GLM (20 ng/mL) and IFX (50 ng/mL). GLM, ADA, IFX and ETA induced apoptosis of monocytes and lymphocytes to a similar degree reaching a level of 23% and ∼40% at 100 μg/mL, respectively. CZP caused no increase in apoptosis above the levels seen with the isotype-matched control. In the neutrophil necrosis assay, ADA,IFX and GLM caused ∼70% necrosis at 100 μg/mL, and ETA 48%. CZP did not increase the level of necrosis above the level of the control. Conclusions: Bioactivity of the IgG1 molecules GLM, IFX and ADA in neutralising human TNF was inferior to that of CZP and ETA. CZP, the only PEGylated anti-TNF, had a different profile to the other anti-TNFs as it was the most potent at inhibiting LPS-driven IL-1β production by monocytes, did not induce apoptosis of activated monocytes and lymphocytes, and did not cause neutrophil necrosis. The clinical relevance of these in vitro effects is unknown. Nevertheless, these assays show interesting in vitro differences between the anti-TNFs. Disclosure statement: G.F. and A.N. are employees of UC

A África, o Sul e as ciências sociais brasileiras : descolonização e abertura

O texto introduz questões recentes sobre a relação entre as ciências sociais na África e no Brasil, inserindo-as no debate sobre as sociologias do Sul e a geopolítica do conhecimento na produção de teoria social. A partir da noção de sociologia não exemplar são apresentados alguns dos possíveis caminhos teórico-metodológicos que possibilitariam um posicionamento mais simétrico para a produção de conhecimento localizada fora da Euro-América.The paper introduces the contemporary debates on the relation of social sciences in Africa and Brazil by framing them both under the current discussion about the "sociologies of the south" and the ones on "the geopolitics of knowledge". Deploying the notion of a "non-exemplary sociology", I seek to present some possible theoretical and methodological ways that would enable a more symmetric positioning of the knowledge produced outside the Euro-America

A parasitic myoma: clinicopathological study of a rare case

Leiomyoma is a benign tumour composed of smooth muscle cells with fibrous stroma and it is the commonest tumour amongst the tumours of uterus. As per FIGO classification system parasitic leiomyoma has no myometrial involvement or uterine attachment. These myomas get detached from the uterus and receive the blood supply from another source. The etiology and pathologic basis of these parasitic fibroids is not yet clearly understood. We report a rare case of parasitic myoma in a 29 years old female patient presented with abdominal discomfort and difficulty in micturition. Clinical examination and subsequent imaging studies revealed a pelvic mass. Histopathological examination of which proved it to be a parasitic myoma. Parasitic myoma is a rare entity which may be iatrogenically created after surgery particularly with morcellation technique. With increasing rates of laparoscopic procedures, surgeons should be aware of the possibility of formation of parasitic myoma and should take intraoperative precautions to minimise its formation

Comparison of the Stress Response and Intubating Performance in Endotracheal Intubation with Macintosh and McCoy Laryngoscopes: A Randomized Study

Introduction: Laryngoscopy and intubation cause stress response and sympathetic stimulation within the body. Attenuation of these changes by pharmacological means and improved technique with modified instruments is of absolute importance, for stable hemodynamics of the patients. Aim: Comparison of stress response to laryngoscopy and intubation, Intubation Difficulty Score (IDS) using Macintosh and McCoy laryngoscopes. Material and methods: After obtaining institutional ethical committee approval and patient consent in this randomized study, sixty patients of ASA grade I and II, posted for elective surgery under general anaesthesia were intubated with Macintosh or McCoy laryngoscopes. Changes in heart rate, mean arterial pressure, systolic and diastolic blood pressure were observed upto 15 minutes after laryngoscopy, along with IDS. Results: McCoy group when compared with Macintosh group had significant reduction in heart rate at T0 (p value <0.0001), systolic blood pressure at T0, T1 and T15 (p value < 0.001), diastolic blood pressure at T0, T10 and T15 (p value <0.05), mean arterial blood pressure at T0, T10 and T15 (p value <0.05) was observed in this study. Conclusion: Hemodynamic stress response with McCoy blade laryngoscope was reduced in magnitude in comparison with Macintosh blade laryngoscope

Methyl-Readers and Inhibitors

Due to their prevalent role in epigenetic gene regulation, methyllysine and methylarginine domain readers have emerged as potential drug targets for small-molecule intervention. Within this book chapter, the biological role and the associated development of potent small molecules inhibiting the protein-protein interaction of methyllysine readers (Tudor, malignant brain tumor, chromo-, and PHD domain) will be discussed. The druggability of these readers and thus their potential to serve as targets for small-molecule ligands will be evaluated critically. Those domains (PWWP, WD40, ankyrin repeats, and ADD domains) which are not yet targeted will be evaluated for their biological actions and eventual therapeutic implications. To sum up, a comprehensive review of the state of the art for all relevant methyl-readers and their inhibitors if present will be given from a medicinal chemistry standpoint of view