Las procesiones de sangre en Sevilla y Nueva España. A propósito de una pintura mural en la iglesia conventual de Huexotzingo

Tomo II ; págs. 314-321

Retablos y esculturas de América : Nuevas aportaciones

Tomo II ; págs. 429-43

El diseño de Juan Bautista Vázquez “el Viejo” para la custodia procesional de Tunja (Colombia)

This article expands the corpus of drawings by Juan Bautista Vázquez ‘the Elder’ that I had the pleasure of inaugurating in 1983 on the pages of BSAA. A new document shows the appearance of the former processional monstrance of Tunja (Colombia), and proves how far its design was indebted to Sevillian liturgical silverwork, whose Renaissance multi-storey baldachin-structures are translated into gilded wood in Vázquez’s work. Quite useful is the recommendation made to the sculptor, asking him to send the monstrance dismantled inside boxes weighing no more than five arrobas, so that they could be easily transported on canoes through the Magdalena River.El presente artículo amplía el corpus de dibujos de Juan Bautista Vázquez “el Viejo” que tuvimos la fortuna de inaugurar en 1983 en las páginas del BSAA. Visualiza el aspecto de la primitiva custodia procesional de Tunja (Colombia) y delata su dependencia de la platería litúrgica sevillana, cuyos templetes renacentistas traduce a madera dorada. Muy práctica es la recomendación que se le hace al escultor de enviar la custodia desarmada en cajones que no pesen más de cinco arrobas para poder transportarlos con facilidad en canoas a través del río Magdalena

Effect of RONS in 6-NBDG/glucose uptake in C2C12 myotubes and single isolated skeletal muscle fibres

[ES] Suplemento de la revista Free Radical Biology and Medicine: Effect of RONS in 6-NBDG/glucose uptake in C2C12 myotubes and single isolated skeletal muscle fibres

H2O2 biosensors HyPer2, HyPer3 and GFP2-Orp1 detect rapid pH changes due to environmental CO2 fluctuations, in addition to intracellular H2O2, in isolated skeletal muscle fibres

[EN] Hydrogen peroxide (H2O2) is one of the Reactive Oxygen Species (ROS) that seems to play an essential role in pathophysiological processes. H2O2 might act as a signaling molecule and modulate different crucial cellular signaling pathways, such as the glucose uptake in skeletal muscle, where H2O2 has been proposed to play an important role. HyPer2, HyPer3 and GFP2-Orp1 are hydrogen peroxide biosensors. We use these biosensors to monitor intracellular H2O2 in single skeletal muscle fibres isolated from the flexor digitorum brevis (FDB) mouse muscle. Previously, the coding sequences of these biosensors were microinjected and electroporated in FDB. Isolated fibres in culture that expressed one of the biosensors were settled incubation chamber coupled to the fluorescence microscope. The chamber maintains temperature (37ºC), environmental CO2 (5%) and humidity. Different time course experimental conditions were performed where fibres were exposed to different agents (insulin, interleukin 1β, H2O2, DTT) and intracellular H2O2 flux was registered in real time using fluorescence microscopy imaging analysis. We observed that when there were environmental CO2 (5%) fluctuations, due to initial medium stabilization or occasional interruption of CO2 supply, the biosensors showed changes in the fluorescence emission, which were registered. The main consequence of CO2 fluctuations is the change in the pH of medium. The main part of the biosensor structure is a fluorescent protein, YFP in de case of HyPer2 and HyPer3, and GFP2 in GFP2-Orp1. It has been reported that these fluorescent proteins are sensitive to pH and this might be a disadvantage for the biosensors. However, we believe that this pH sensitivity should be considered as an additional property of this biosensors, since they provide information in real time about the rapid changes of pH due to environmental fluctuation of CO2 and likely other gases such as O2 or N2

Effect of aging in functional redox state of single isolated skeletal muscle fibres

[EN] Skeletal muscle constantly produces reactive oxygen species (ROS). During contractile activity ROS are generated in skeletal muscle fibres. There is considerable support for an involvement of ROS in the process of aging. Several studies indicate that adaptive responses of skeletal muscle that are activated and regulated by ROS are disrupted during aging. The aim of this study was to monitor, in real time, intracellular ROS production in single skeletal muscle fibres from old and young mice and study the effect of contractile activity in these cells. Following evaluate and correlate the potential changes in intracellular ROS production with glutathione redox state and antioxidant enzymatic activities in muscle. Single skeletal muscle fibres were isolated from the Flexor Digitorus Brevis muscle from young (2-4 monthold) and old (26-28 month-old) C57BL/6 mice. Fibres were loaded with DCFH-DA, a fluorophore probe that allows the quantification of intracellular ROS generation by fluorescence microscopy imaging. Contractile activity was induced in fibres by electrical stimulation. Glutathione redox state and activity of antioxidant enzymes were analysed in gastrocnemious muscle. Intracellular basal level of ROS was higher in fibres from old mice. Contractile activity induced increase of ROS generation in fibres from young mice. However, this response was attenuated in fibres from old mice. Glutathione redox state was significant different, in favour of oxidized glutathione, in muscles from old mice. Glutathione peroxidase and catalase activities were significantly augmented in muscles from old mice. In conclusion, the process of aging modifies the basal redox status in skeletal muscle fibres in favour of oxidation and induces adaptation mechanisms of antioxidant defences. These are not able to neutralize the increase of basal oxidation, but they might lead to the attenuation of ROS produced by contractile activity observed in fibres from old mice

HyPer biosensor to monitor intracellular hydrogen peroxide in skeletal muscle cells

[EN] Suplemento de la revista Free Radical Biology and Medicine: HyPer biosensor to monitor intracellular hydrogen peroxide in skeletal muscle cells

Reactive oxygen species and loss of muscle fibres during ageing

[ES] Sumario de la revista Neuromuscular Disorders: Especies reactivas de oxígeno y pérdida de fibras musculares durante el envejecimiento