337 research outputs found
Herbivoria de Tetranychus urticae Koch (Acari: tetranychidae) induz defesa direta em morangueiro?
Plantas de morangueiro Fragaria x ananassa podem apresentar mecanismos de defesa direta e indireta contra herbívoros. Tais defesas podem alterar o comportamento e o desenvolvimento dos ácaros fitófagos e seus inimigos naturais, reduzindo a taxa de herbivoria. Testou-se o efeito da préinfestação de plantas de morangueiro por Tetranychus urticae Koch sobre o seu desenvolvimento e reprodução. Plantas de morangueiro cultivar IAC Campinas foram divididas em dois grupos: plantas limpas e plantas pré-infestadas por T. urticae. A infestação prévia de plantas de morangueiro por T. urticae não alterou a duração das fases imaturas, a fecundidade e a sobrevivência das fêmeas do ácaro. Tais resultados podem estar associados à inexistência ou baixo nível de resistência induzida da cultivar IAC Campinas, ou ainda, ao fato de a pré-infestação ter sido insuficiente para induzir defesa. Assim, a resistência relatada na cultivar IAC Campinas a T. urticae pode estar relacionada às defesas indiretas
Ácaros associados ao cafeeiro em duas regiões do sul de Minas Gerais.
Existe uma grande complexidade nas populações de ácaros associados a cultura do café e o rompimento do equilíbrio dessas populações tem contribuído para o aparecimento de surtos de ácaros fitófagos, que vem sendo observados em varias regiões de cultivo. Para se verificar quais são os ácaros que compõem as populações de predadores e fitófagos, em equilíbrio natural na cultura, conduziu-se um estudo nos municípios de Lavras e Machado no período de marco de 1989 a marco 1990. Os cargos foram escolhidos em áreas que não recebiam nenhum tipo de tratamento fitossanitário. Em intervalos de 15 dias, 3 folhas por quadrante foram coletadas do terco superior interno de cada uma de dez plantas tomadas aleatoriamente em cada area de estudo. O material foi levado para o laboratório de Entomologia do Departamento de Fitossanidade-ESAL, onde todos os ácaros existentes em cada folha eram contados e coletados. As espécies coletadas nos dois campos estudados foram: Brevipalpus phoenicis (Geijskes), Iorrya formosa Cooreman, Tydeus (Tydeus) sp., Olegonychus ilicis (McGregor), Fungitarsonemus sp., Parapronematus sp., Paracheyletia aff. wellsi (Baker), Bdella sp., Zetzellia sp., Iphiseiodes zuluagai Denmark & Muma, Euseius citrifolius Denmark & Muma, E. concordis (Chant) E. alatus (DeLeon), Amblyseius herbicolus (Chant), Proprioseiopsis dominigos (El-Benhawy), Metaseiulus annectens (DeLeon)
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Interspecies differences in protein expression do not impact the spatiotemporal regulation of glycoprotein VI mediated activation
Background
Accurate protein quantification is a vital prerequisite for generating meaningful predictions when using systems biology approaches, a method that is increasingly being used to unravel the complexities of sub cellular interactions and as part of the drug discovery process. Quantitative proteomics, flow cytometry and western blotting have been extensively used to define human platelet protein copy numbers, yet for mouse platelets, a model widely used for platelet research, evidence is largely limited to a single proteomic dataset in which the total amount of proteins were generally comparatively higher than those found in human platelets.
Objectives
To investigate the functional implications of discrepancies between levels of mouse and human proteins in the GPVI signalling pathway using a systems pharmacology model of GPVI
Methods
The protein copy number of mouse platelet receptors was determined using flow cytometry. The Virtual Platelet, a mathematical model of Glycoprotein VI (GPVI) signalling, was used to determine the consequences of protein copy number differences observed between human and mouse platelets.
Results and conclusion
Despite the small size of mouse platelets compared to human platelets they possessed a greater density of surface receptors alongside a higher concentration of intracellular signalling proteins. Surprisingly the predicted temporal profile of Syk activity was similar in both species with predictions supported experimentally. Super resolution microscopy demonstrates that the spatial distribution of Syk is similar between species, suggesting that the spatial distribution of receptors and signalling molecules in activated platelets, rather than their copy number, is important for signalling pathway regulation
Sibling relationships and family functioning in siblings of early adolescents, adolescents and young adults with autism spectrum disorder
The purpose of the study was to investigate how family functioning (defined as the ability that family members hold to manage stressful events, and intimate and social relationships), the degree to which family members feel happy and fulfilled with each other (called family satisfaction), and the demographical characteristics of siblings (age and gender) impacted on sibling relationships. The Circumplex Model of Marital and Family Systems and Behavioral Systems constituted the theoretical frameworks that guided our study. Eighty-six typically developing adolescents and young adults having a sister or a brother with autism spectrum disorder were enrolled. Results indicated that the youngest age group (early adolescents) reported to engage more frequently in negative behaviors with their siblings with ASD than the two older age groups (middle adolescents and young adults). No significant differences were found among the three age groups regarding behaviors derived from attachment, caregiving and affiliative systems. Family satisfaction and age significantly predicted behaviors during sibling interactions. Suggestions on prevention and intervention programs were discussed in order to prevent parentification among typically developing
siblings and decrease episodes of quarrels and overt conflicts between brothers and sisters with and without AS
Manipulação do habitat em diferentes escalas espaciais para o controle biológico conservativo em hortaliças orgânicas.
Suplemento. Edição dos Anais do 10 Congresso Brasileiro de Agroecologia; 6 Congresso Latino-americano de Agroecologia; 5 Seminário de Agroecologia do Distrito Federal e Entorno, Brasília, DF, set. 2017. Na publicação: Sujii, Edison; Souza, Lucas; Sousa, Ale
Spider mite web mediates anti-predator behaviour
Herbivores suffer significant mortality from predation and are therefore subject to natural selection on traits promoting predator avoidance and resistance. They can employ an array of strategies to reduce predation, for example through changes in behaviour, morphology and life history. So far, the anti-predator response studied most intensively in spider mites has been the avoidance of patches with high predation risk. Less attention has been given to the dense web produced by spider mites, which is a complex structure of silken threads that is thought to hinder predators. Here, we investigate the effects of the web produced by the red spider mite, Tetranychus evansi Baker & Pritchard, on its interactions with the predatory mite, Phytoseiulus longipes Evans. We tested whether female spider mites recognize predator cues and whether these can induce the spider mites to produce denser web. We found that the prey did not produce denser web in response to such cues, but laid more eggs suspended in the web, away from the leaf surface. These suspended eggs suffered less from predation by P. longipes than eggs that were laid on the leaf surface under the web. Thus, by altering their oviposition behaviour in response to predator cues, females of T. evansi protect their offspring
A Herbivorous Mite Down-Regulates Plant Defence and Produces Web to Exclude Competitors
Herbivores may interact with each other through resource competition, but also through their impact on plant defence. We recently found that the spider mite Tetranychus evansi down-regulates plant defences in tomato plants, resulting in higher rates of oviposition and population growth on previously attacked than on unattacked leaves. The danger of such down-regulation is that attacked plants could become a more profitable resource for heterospecific competitors, such as the two-spotted spider mite Tetranychus urticae. Indeed, T. urticae had an almost 2-fold higher rate of oviposition on leaf discs on which T. evansi had fed previously. In contrast, induction of direct plant defences by T. urticae resulted in decreased oviposition by T. evansi. Hence, both herbivores affect each other through induced plant responses. However, when populations of T. evansi and T. urticae competed on the same plants, populations of the latter invariably went extinct, whereas T. evansi was not significantly affected by the presence of its competitor. This suggests that T. evansi can somehow prevent its competitor from benefiting from the down-regulated plant defence, perhaps by covering it with a profuse web. Indeed, we found that T. urticae had difficulties reaching the leaf surface to feed when the leaf was covered with web produced by T. evansi. Furthermore, T. evansi produced more web when exposed to damage or other cues associated with T. urticae. We suggest that the silken web produced by T. evansi serves to prevent competitors from profiting from down-regulated plant defences
Sonic hedgehog is expressed in human brain arteriovenous malformations and induces arteriovenous malformations in vivo
Abnormalities in arterial versus venous endothelial cell identity and dysregulation of angiogenesis are deemed important in the pathophysiology of brain arteriovenous malformations (AVMs). The Sonic hedgehog (Shh) pathway is crucial for both angiogenesis and arterial versus venous differentiation of endothelial cells, through its dual role on the vascular endothelial growth factor/Notch signaling and the nuclear orphan receptor COUP-TFII. In this study, we show that Shh, Gli1 (the main transcription factor of the Shh pathway), and COUP-TFII (a target of the non-canonical Shh pathway) are aberrantly expressed in human brain AVMs. We also show that implantation of pellets containing Shh in the cornea of Efnb2/LacZ mice induces growth of distinct arteries and veins, interconnected by complex sets of arteriovenous shunts, without an interposed capillary bed, as seen in AVMs. We also demonstrate that injection in the rat brain of a plasmid containing the human Shh gene induces the growth of tangles of tortuous and dilated vessels, in part positive and in part negative for the arterial marker \u3b1SMA, with direct connections between \u3b1SMA-positive and -negative vessels. In summary, we show that the Shh pathway is active in human brain AVMs and that Shh-induced angiogenesis has characteristics reminiscent of those seen in AVMs in humans
Comparison of the GPVI inhibitors losartan and honokiol
<p>Losartan and honokiol are small molecules which have been described to inhibit aggregation of platelets by collagen. Losartan has been proposed to block clustering of GPVI but not to affect binding of collagen. Honokiol has been reported to bind directly to GPVI but only at a concentration that is three orders of magnitude higher than that needed for inhibition of aggregation. The mechanism of action of both inhibitors is so far unclear. In the present study, we confirm the inhibitory effects of both agents on platelet aggregation by collagen and show that both also block the aggregation induced by the activation of CLEC-2 or the low affinity immune receptor FcγRIIa at similar concentrations. For GPVI and CLEC-2, this inhibition is associated with a reduction in protein tyrosine phosphorylation of multiple proteins including Syk. In contrast, on a collagen surface, spreading of platelets and clustering of GPVI (measured by single molecule localisation microscopy) was not altered by losartan or honokiol. Furthermore, in flow whole-blood, both inhibitors suppressed the formation of multi-layered platelet thrombi at arteriolar shear rates at concentrations that hardly affect collagen-induced platelet aggregation in platelet rich plasma. Together, these results demonstrate that losartan and honokiol have multiple effects on platelets which should be considered in the use of these compounds as anti-platelet agents.</p
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