A lambda/plasmid Cre/lox hybrid vector for large genomic (18kb) fragment insertions and fungal genomic library construction

We have previously constructed lambda/plasmid hybrid vectors designed for both fungal cDNA and genomic library construction (Brunelli and Pall, 1994 Fungal Genet. Newslet. 41:63-65). The genomic library inserts, however, were limited to about 11 kb in size due to the size limitations of lambda packaging. We have constructed a similar vector that has three advantages over these earlier hybrid vectors, as discussed further below. The plasmid pBARGEM7-2 (Pall and Brunelli, 1993. Fungal Genet. Newslet. 40:59-61) was modified by inserting a stuffer sequence into the BamHI site of the polylinker. The stuffer sequence was about 6 kb and can be cut out with BamHI, yielding two BamHI fragments of about 4.5 kb and 1.5 kb. The 4.5 kb fragment contains the lacZ gene, producing very blue colonies (or plaques in lambda) on Xgal medium

New plasmid and lambda/plasmid hybrid vectors and a Neurospora crassa genomic library containing the bar selectable marker and the Cre/lox site-specific recombination system for use in filamentous fungi

In the previous Fungal Genetics Newsletter, we described a series of plasmid vectors constructed carrying the bar gene as a selectable marker for use in filamentous fungi (Pall and Brunelli 1993 Fungal Genetics Newsl. 40:59-63; Pall 1993 Fungal Genetics Newsl. 40:58). In this note, we describe an additional plasmid expression vector carrying this selectable marker and the construction of four llambda/plasmid hybrid vectors carrying the bar gene within plasmid inserts that can excise by Cre/lox-mediated excision. A Neurospora crassa genomic library constructed in one of these lambda/plasmid hybrid vectors is also described below

A series of six compact fungal transformation vectors containing polylinkers with multiple unique restriction sites

In comparison with transformation vectors available for use in E. coli or yeast, there has been relatively little development of vectors for use in filamentous fungi. For example, expression yeast vectors carrying polylinkers flanked by promoters and terminators are available for various uses but such vectors have not been in the public domain for researchers working with filamentous fungi

Elevation gradients of European climate change in the regional climate model COSMO-CLM

A transient climate scenario experiment of the regional climate model COSMO-CLM is analyzed to assess the elevation dependency of 21st century European climate change. A focus is put on near-surface conditions. Model evaluation reveals that COSMO-CLM is able to approximately reproduce the observed altitudinal variation of 2m temperature and precipitation in most regions and most seasons. The analysis of climate change signals suggests that 21st century climate change might considerably depend on elevation. Over most parts of Europe and in most seasons, near-surface warming significantly increases with elevation. This is consistent with the simulated changes of the free-tropospheric air temperature, but can only be fully explained by taking into account regional-scale processes involving the land surface. In winter and spring, the anomalous high-elevation warming is typically connected to a decrease in the number of snow days and the snow-albedo feedback. Further factors are changes in cloud cover and soil moisture and the proximity of low-elevation regions to the sea. The amplified warming at high elevations becomes apparent during the first half of the 21st century and results in a general decrease of near-surface lapse rates. It does not imply an early detection potential of large-scale temperature changes. For precipitation, only few consistent signals arise. In many regions precipitation changes show a pronounced elevation dependency but the details strongly depend on the season and the region under consideration. There is a tendency towards a larger relative decrease of summer precipitation at low elevations, but there are exceptions to this as wel

Warming shifts the biomass distribution of soil microarthropod communities

Climate warming is often more detrimental to large body sized organisms than small body sized organisms. Yet, how such differential effects of warming at organismal levels affect aggregate community properties, such as community biomass, remains little understood. Here, using geothermally warmed sub-Arctic grassland soils, we investigate how total biomass (product of density and individual body mass) of two major groups of soil microarthropods (Collembola and mites), which are composed of both large and small body sized species, shift in warmed soils when warmed by ∼3–∼6 °C. Our results show that total biomass of Collembola significantly decreased in warmed soils predominantly due to a decline in the density of large body sized species. In contrast, total mite biomass showed a unimodal response to warming. As a result, there was a shift towards mite biomass dominated microarthropod communities in warmed soils. Within Collembola, the deep soil living eu-edaphic functional group declined the most in total biomass, whereas the unimodal response in mites was most pronounced in oribatid mites. Our study highlights that warming induced shifts in total community biomass of soil microarthropods are likely due to greater detrimental effects of warming on several large body sized Collembola

The high A_V Quasar Survey: A z=2.027 metal-rich damped Lyman-alpha absorber towards a red quasar at z=3.21

To fully exploit the potential of quasars as probes of cosmic chemical evolution and the internal gas dynamics of galaxies it is important to understand the selection effects behind the quasar samples and in particular if the selection criteria exclude foreground galaxies with certain properties (most importantly a high dust content). Here we present spectroscopic follow-up from the 10.4-m GTC telescope of a dust-reddened quasar, eHAQ0111+0641, from the extended High A_V Quasar (HAQ) survey. We find that the z=3.21 quasar has a foreground Damped Lyman-alpha Absorber (DLA) at z=2.027 along the line of sight. The DLA has very strong metal lines due to a moderately high metallicity (with an inferred lower limit of 25% of the solar metallicity), but a very large gas column density along the line-of-sight in its host galaxy. This discovery is further evidence that there is a dust bias affecting the census of metals, caused by the combined effect of dust obscuration and reddening, in existing samples of z>2 DLAs. The case of eHAQ0111+0641 illustrates that dust bias is not only caused by dust obscuration, but also dust reddening.Comment: 7 pages, 4 figures. Accepted for publication in A&A. A few typos have been correcte

Neural correlates of executive function and working memory in the 'at risk mental state'

Background and Aims: People with ‘prodromal’ symptoms have a very high risk of developing psychosis. We used functional MRI to examine the neurocognitive basis of this vulnerability. Method: Cross-sectional comparison of subjects with an ARMS (n=17), first episode schizophreniform psychosis (n=10) and healthy volunteers (n=15). Subjects were studied using functional MRI while they performed an overt verbal fluency task, a random movement generation paradigm and an N-Back working memory task. Results: During an N-Back task the ARMS group engaged inferior frontal and posterior parietal cortex less than controls but more than the first episode group. During a motor generation task, the ARMS group showed less activation in the left inferior parietal cortex than controls, but greater activation than the first episode group. During verbal fluency using ‘Easy’ letters, the ARMS group demonstrated intermediate activation in the left inferior frontal cortex, with first episode groups showing least, and controls most, activation. When processing ‘Hard’ letters, differential activation was evident in two left inferior frontal regions. In its dorsolateral portion, the ARMS group showed less activation than controls but more than the first episode group, while in the opercular part of the left inferior frontal gyrus / anterior insula activation was greatest in the first episode group, weakest in controls and intermediate in the ARMS group. Conclusions: The ARMS is associated with abnormalities of regional brain function that are qualitatively similar to those in patients who have just developed psychosis but less severe

Multi-satellite observed responses of precipitation and its extremes to interannual climate variability

The consistency of precipitation variability estimated from the multiple satellite-based observing systems is assessed. There is generally good agreement between TRMM TMI, SSM/I, GPCP and AMSRE datasets for the inter-annual variability of precipitation since 1997 but the HOAPS dataset appears to overestimate the magnitude of variability. Over the tropical ocean the TRMM 3B42 dataset produces unrealistic variabilitys. Based upon deseasonalised GPCP data for the period 1998-2008, the sensitivity of global mean precipitation (P) to surface temperature (T) changes (dP/dT) is about 6%/K, although a smaller sensitivity of 3.6%/K is found using monthly GPCP data over the longer period 1989-2008. Over the tropical oceans dP/dT ranges from 10-30%/K depending upon time-period and dataset while over tropical land dP/dT is -8 to -11%/K for the 1998-2008 period. Analyzing the response of the tropical ocean precipitation intensity distribution to changes in T we find the wetter area P shows a strong positive response to T of around 20%/K. The response over the drier tropical regimes is less coherent and varies with datasets, but responses over the tropical land show significant negative relationships over an interannual time-scale. The spatial and temporal resolutions of the datasets strongly influence the precipitation responses over the tropical oceans and help explain some of the discrepancy between different datasets. Consistency between datasets is found to increase on averaging from daily to 5-day time-scales and considering a 1o (or coarser) spatial resolution. Defining the wet and dry tropical ocean regime by the 60th percentile of P intensity, the 5-day average, 1o TMI data exhibits a coherent drying of the dry regime at the rate of -20%/K and the wet regime becomes wetter at a similar rate with warming