Coarse Graining RNA Nanostructures for Molecular Dynamics Simulations

A series of coarse-grained models have been developed for the study of the molecular dynamics of RNA nanostructures. The models in the series have one to three beads per nucleotide and include different amounts of detailed structural information. Such a treatment allows us to reach, for the systems of thousands of nucleotides, a time scale of microseconds (i.e. by three orders of magnitude longer than in the full atomistic modelling) and thus to enable simulations of large RNA polymers in the context of bionanotechnology. We find that the 3-beads-per-nucleotide models, described by a set of just a few universal parameters, are able to describe different RNA conformations and are comparable in structural precision to the models where detailed values of the backbone P-C4' dihedrals taken from a reference structure are included. These findings are discussed in the context of the RNA conformation classes

Мікробіологічна оцінка ефективності сучасних антисептиків, антимікробних матеріалів

Effectiveness of antiseptics decasan®(DC), chlorhexidine digluconate (CH), miramistin (MR), antimicrobial composition of decamethoxin (AMC, patent N 74853, Ukraine), antimicrobial dressings against isolated strains of S. aureus (n 32), E. coli (n 25), P. aeruginosa (n 20), C. albicans (n 16) in patients with diabetes having pyo-inflammatory complications has been researched. The antimicrobial properties of antiseptics have been studied by means of the serial dilutions method. The antimicrobial activity of dressings (1.0x1.0 cm), such as medical cotton impregnated with AMC; antiseptic overlay with CH (AOCH); Traumastem Biodress Disinfect®(TBD); activtex CH®, activtex CHF®, against clinical isolates of microorganisms has been studied on solid media. The bactericidal action against S. aureus in the presence of AMC (1.4±0.2 mkg/ml), DC (1.73±0.2 mkg/ml); CH (12.8±2.1 mkg/ml); MR (8.3±0.9 mkg/ml) has been found. The bactericidal properties of DC and MR in relation to E. coli in their concentrations of 6.68±0.71 and 17.9±1.9 mkg/ml, respectively, have been determined. AMC (4.9±0.5 mkg/ml) was six times more active than CH (p<0.001). The antipseudomonal action of DC against P. aeruginosa was 1.5 times higher than CH. AMC had also 2.8 times higher activity (p<0.001). The bactericidal action of MR was registered in the presence of 72.9±2.2 mkg/ml. It has been found that C. albicans is sensitive to AMC (7.4±1.9 mkg/ml), DC (14.6±1.9 mkg/ml), MR (26.0±3.6 mkg/ml). CH has a low effectiveness in relation to C. albicans (32.8±7.4 mkg/ml). Advantages of the antimicrobial activity of modern antimicrobial dressings with AMC against S. aureus, E. coli, P. aeruginosa, fungi of Candida genus have been found.Исследовали эффективность антисептиков декасана (ДС), хлоргексидина биглюконата (ХГ), мирамистина (МР), антимикробной композиции декаметоксина (АМК, пат. №74853, Украина), противомикробных перевязочных материалов в отношении выделенных штаммов микроорганизмов S. aureus (n 32), E. coli (n 25), P. аureginosa (n 20), C. albicans (n 16) от больных сахарным диабетом с гнойно-воспалительными осложнениями. Противомикробные свойства антисептиков изучали методом серийных двукратных разведений. Противомикробную активность образцов (1,0x1,0 см) медицинской хлопчатобумажной ткани, импрегнированной АМК; официнальных перевязочных материалов: салфеток антисептических с хлоргексидином (САХ); Traumastem Biodress Disinfect® (TBD); активтекс® Х; активтекс®ХФ на клинические изоляты микроорганизмов изучали на плотных питательных средах. Установлено бактерицидное действие на S. aureus в присутствии АМК (1,4±0,2 мкг/мл); ДС (1,73±0,2 мкг/мл); ХГ (12,8±2,1 мкг/мл); МР (8,3±0,9 мкг/мл). Бактерицидные свойства ДС и МР в отношении E. соli определяли в присутствии 6,68±0,71 мкг/мл и 17,9±1,9 мкг/мл соответственно. АМК (4,9±0,5 мкг/мл) действовала в 6 раз активнее на E. соli по сравнению с ХГ (р<0,001). Антипсевдомонадное действие ДС было в 1,5 раза выше (79,2±7,4 мкг/мл), а у АМК в 2,8 раза выше, чем у ХГ (р<0,001). Бактерицидное действие МР в отношении P. aeruginosa установили в количестве 72,9±2,2 мкг/мл. Установлена чувствительность C. albicans к АМК (7,4±1,9 мкг/мл), ДС (14,6±1,9мкг/мл), мирамистина (26,0±3,6 мкг/мл). ХГ выявил низкую эффективность в отношении C. albicans (32,8±7,4 мкг/мл). Определены преимущества противомикробного действия современных антимикробных перевязочных материалов с АМК в отношении S. aureus, E. coli, P. aeruginosa, C. albicans (р<0,05).Досліджена ефективність антисептиків декасану (ДС), хлорогексидину біглюконату (ХГ), мірамістину (МР), антимікробної композиції декаметоксину (АМК, пат. №74853, Україна), антимікробних перев’язувальних матеріалів щодо виділених штамів мікроорганізмів S. aureus (n 32), E. coli (n 25), P. аureginosa (n 20), C. albicans (n 16) від хворих на цукровий діабет з гнійно-запальними ускладненнями. Антимікробні властивості антисептиків вивчали методом серійних двократних розведень. Антимікробну активність зразків (1,0x1,0 см) медичної бавовни, імпрегнованої АМК; офіцинальних перев’язувальних матеріалів: серветок антисептичних з хлорогексидином (САХ); Traumastem Biodress Disinfect®(TBD); активтекс®Х; активтекс®ХФ на клінічні ізоляти мікроорганізмів вивчали на щільних поживних середовищах. Встановлено бактерицидну дію на S. aureus в присутності АМК (1,4±0,2 мкг/мл); ДС (1,73±0,2 мкг/мл); ХГ (12,8±2,1 мкг/мл); МР (8,3±0,9 мкг/мл). Бактерицидні властиво- сті ДС і МР щодо E. соli визначали в присутності 6,68±0,71 та 17,9±1,9 мкг/мл відповідно. АМК (4,9±0,5 мкг/мл) була в 6 разів активнішою щодо E. соli порівняно з ХГ (р<0,001). Антипсевдомонадна дія ДС була в 1,5 рази вищою (79,2±7,4 мкг/мл), а АМК у 2,8 рази вищою, ніж у ХГ (р<0,001). Бактерицидну дію МР щодо P. aeruginosa встановили в присутності 72,9±2,2 мкг/мл. Встановлено чутливість C. albicans до АМК (7,4±1,9 мкг/мл), декасану (14,6±1,9мкг/мл), мірамістину (26,0±3,6 мкг/мл). ХГ виявив низьку ефективність щодо C. albicans (32,8±7,4 мкг/мл). Визначені переваги антимікробної дії сучасних антимікробних перев’язувальних матеріалів з АМК щодо S. aureus, E. coli, P. aeruginosa, C. albicans (р<0,05)

Endoparasitoses of the Eurasian collared dove (Streptopelia decaocto) on the northern Black Sea coast of Ukraine

One of the main tasks of ecological parasitology at the present stage is to determine patterns of spread of invasive diseases of poultry in the environment through a thorough epidemiological examination, as well as to determine the main directions and factors of spread of parasites in Ukraine. Some pigeon health problems can affect their populations, but parasitic infections play an important role. Our research found that 83.3% of Eurasian collared doves examined (Streptopelia decaocto) were affected by endoparasites. The commonest helminths were Raillietina spp. with the average invasion intensity of 4.9 ± 1.3 specimens/ind., Baruscapillaria spp. and Ascaridia columbae with an invasion intensity of 4.6 ± 1.5 and 3.7 ± 2.1 specimens/ind., respectively. The commonest cestodes were Raillietina spp. with the extent of invasion of 52.2% and Davainea proglottina – 6.7%. The Amoebotaenia cuneata cestodes were recorded in 3 (3.3%) doves, and Echinolepis carioca – only in 2 (2.2%) doves. Six doves (6.7%) were infected with the nematode Ascaridia columbae and 5 (5.6%) – Baruscapillaria spp. Eimerian oocysts were found in 9 (10%) collared doves, and Trichomonas gallinae was recorded in 15 (16.7%) birds. According to the results of helminthological autopsy and identification of the isolated pathogens, 8 varieties of mixinvasions were identified. In association with the Rayetins, there were the protozoa Eimeria spp. and Trichomonas gallinae and the nematode Ascaridia columbae. Two-component invasions were registered in 78.6% of doves, three-component infestations in 14.3% and four-component infestations in 7.1% of birds. Mixed infection with Raillietina spp. + Ascaridia columbae was found in the small intestine of Streptopelia decaocto, which was also pathomorphologically observed to have catarrhal-hemorrhagic enteritis, which was accompanied by the formation of a large number of spotted hemorrhages on the mucous membrane, and in the places of attachment of cestodes - necrotic-caseous areas. Temperature and other weather conditions such as humidity, precipitation, etc. of the northern Black Sea coast affect the population size of intermediate host cestodes. Continuous monitoring of invasive diseases of pigeons of different species will help to take the necessary preventive measures against endoparasitoses of birds

Space and time in the context of social measurement

This paper discusses some of the basic philosophical concepts – the concepts of “space and time” - and their relation to social evolution. The work presents the characteristics of social forms of space and time, shows the specifics of their formation in connection with human activity and the system of social relations. It was found that time duration has different manifestations in the context of historically different cultural epochs. Acceleration of social time takes place in the course of human historical practice development. Meanwhile, some social systems sometimes take the form of a certain deceleration in time. This usually occurs because of the inadequacy of the control system for the capabilities of the social organism. Social space is primarily connected with the dynamics of changes in social bonds and relationships. In the process of historical formation of social systems, social space assumes a more complex structure and expands. In the period of globalization growth, social space takes the form of a truly global integral system.peer-reviewe

Parasites of domestic and wild pigeons in the south of Ukraine

Pigeons are closely related to human life and are both a source of food and object for hobbies and sports. Parasitic diseases of birds are the main reason for their growth retardation, reduced productivity and viability. The article presents the results of studying the prevalence of trichomonosis, cestodes and nematodes among the population of wild and domestic pigeons in the south of Ukraine. According to the results of the conducted researches it is established that in the south of Ukraine endoparasitoses of domestic and wild pigeons are quite widespread, and all species of this bird are carriers of Trichomonas gallinae. At the same time, the highest rate of Trichomonas infection was recorded in Columba palambus and C. livia domestica, and the overall infestation of males is higher by 4.4% compared to females. It was determined that domestic pigeons C. livia domestica were infested with trichomonosis (27.5%) and nematodes (Ascaridia columbae, 57.1%), C. palambus – trichomonosis (32.1%) and cestodes (Raillietina spp., 82.4%), Streptopelia turtur – cestodes (Raillietina spp., 12.5%) and S. decaocto – cestodes (Raillietina spp., 71.4%) and nematodes (Heterakis gallinarum, A. columbae – 33.3% and 44.4% respectively). The overall rate of infestation of pigeons with cestodes was 28.4% and the incidence in males was higher by 3.9% compared to females. It was found that the most common species among pigeon cestodes is Raillietina spp. In addition, 22.9% of pigeons are carriers of nematodes (H. gallinarum, A. columbae and Capillaria spp.). Continuous monitoring of pigeon parasites is necessary because they, in most cases, come into contact with other species of poultry and are a source of general invasion. In terms of further research, it would be promising to study the prevalence of helminthic infestation among wild migratory birds

Endoparasites of rabbits (Oryctolagus cuniculus domesticus) in Southern Ukraine

One of the main tasks of ecological parasitology at the present stage is to establish patterns of spread of invasive diseases of animals in the environment through a thorough epidemiological examination, as well as to determine the main directions and factors of spread of parasites in Ukraine. Among the many pathogens, endoparasites play a major role in a number of diseases in rabbits with increased morbidity and mortality. In the body of both wild and domestic rabbits several species of parasites that form a parasitocenosis can be localized. The latter have a pathogenic effect on organs and tissues, leading to reduced weight gain, premature slaughter and even mortality. The prevalence of endoparasitoses was studied in weaned rabbits 60 days of age, 120 days of age at fattening, 180 days of age – mating age and adults of 320 days of age to determine the extensiveness and intensity of the dominant invasion. A total of 720 head was studied, 180 animals from each age group. Three species of nematodes were recorded in the rabbits: Trichostrongylus retortaeformis (Zeder, 1800), Nematodirus leporis (Ransom, 1907) and Passalurus ambiguus (Rudolphi, 1819); the cestode Taenia pisiformis (Bloch, 1780); three species of Emeria: Eimeria stiedae (Lindermann, 1865), which parasitizes in the bile ducts of the liver and gallbladder, E. magna (Perard, 1925) and E. media (Kessel, 1929) – in the epithelial cells of the intestine. The prevalence of the infestation depends on the age of the animals. According to the data obtained, eimeriosis was one of the main parasitic diseases of the rabbits. Dominant invasions of Eimeria in the intestines of 60-day-old rabbits were found in the studied animals, their extensiveness reached 19.4%, while the spread of hepatic Eimeria was registered in 13.3% of the examined rabbits. The total infestation of weaned rabbits with helminths was 22.2%, in rabbits for fattening – 26.7%, and in rabbits of mating age and adults – 20.0% and 20.6%, respectively. Global climate change will change the distribution and dynamics of soil-borne helminthiases, but host immunity may also affect host-parasite interactions. Subsequent studies will be aimed at elucidating the effect of mono and mixed invasions on the body of rabbits. Updated data on helminthiasis will expand the screening strategy to maintain rabbit health and reduce economic losses

Dynamical transitions in correlated driven diffusion in a periodic potential

The diffusion of a two-dimensional array of particles driven by a constant force in the presence of a periodic external potential exhibits a hierarchy of dynamical phase transitions when the driving force is varied. This behavior can be explained by a simple phenomenological approach which reduces the system of strongly interacting particles to weakly interacting quasi-particles (kinks). The richness of the strongly coupled system is however not lost because, contrary to a single-Brownian particle, the array shows an hysteretic behavior even at non-zero temperature. The present investigation can be viewed as a first step toward understanding nanotribology.Comment: 4 pages, 3 pictures, revtex to appear in Phys Rev. Let

Становление и развитие категорий возможности и действительности

Палий О. Н. Становление и развитие категорий возможности и действительности / О. Н. Палий // Актуальні проблеми держави і права : збірник наук. праць. Вип. 65 / НУ «ОЮА» , редкол. С. В. Ківалов (голов. ред.) та ін., відп. за вип. В. М. Дрьомін. – Одеса : Юрид. л-ра, 2012. – С. 224-231.Статья посвящена исследованию возникновения и развития категорий действительности и возможности. Автор считает, что с помощью данных категорий возможно полнее познать сущность и механизмы развития правовых явлений и более успешно решать многие проблемы в сфере правоприменения

RESEARCH IN SENSITIVITY TO ANTIBIOTICS, ANTISEPTICS IN PSEUDOMONAS AERUGINOSA STRAINS ISOLATED FROM PATIENTS WITH INFECTIOUS COMPLICATIONS

Background. Infections caused by Pseudomonas are one of the topical issues of medicine.Objective. The aim of the research was to study sensityvity to antibiotics, antiseptics of P. aeruginosa clinical strains that cause infectious complications in patients with burns.Methods. Microbiological study of biological material, received from 435 patients with burns of the 3rd-4th stages (2011-2015 years). In early terms of burn disease 127 clinical strains of P. aeruginosa were isolated from patients. Standard methods were used to identify clinical isolates of P. aeruginosa by their morphological, tinctirial, culture and biochemical properties. The research of antimicrobial action of antiseptics, antibiotics against Pseudomonas were carried out by means of standard methods according to the Directive of the Ministry of Health of Ukraine (No. 167 from 05.04.2007 р.) and guidelines of National Committee of Clinical and Laboratory Study (NCCLS, 2002).Results. It was established that P. aeruginosa caused infectious complications in 23.9% of patients among other pathogens. Clinical isolates of P. aeruginosa were found to be low sensitive to amoxicillin/clavulanate (30.76%), ceftazidime (25.92%), cefoperazonum/sulbactam (46.15%), aztreonam (51.85%), tobramycin (38.46%), amicacin (70.34%), doxiciclini (26.92%), fluoroquinolones (59.26%). The analitical progistic criteria of decrease of sensitivity to ceftazidime, cefepim, meropenem and gatifloxacin were found in P. aeruginosa. This pathogen was determined to be sensitive to decasan ®, antimicrobial composition of decamethoxine ®, iodine pvidone.Conclusions. Clinical strains of Pseudomonas aeruginosa, being highly resistant to antibiotics, are also very sensitive to antiseptics decasan ®, antimicrobial of decamethoxine®, povidone iodine

Диспозитивность правовых норм

Палий О. Н. Диспозитивность правовых норм / О. Н. Палий // Правове життя сучасної України : матеріали Міжнар. наук. конф. проф.-викл. та аспірант. складу (м. Одеса, 16-17 травня 2013 р.) / відп. за вип. В. М. Дрьомін ; НУ "ОЮА". Півд. регіон. центр НАПрН України. - Одеса : Фенікс, 2013. - Т. 1. - С. 41-43