Conversion of DNA sequences: From a transposable element to a tandem repeat or to a gene

Eukaryotic genomes are rich in repetitive DNA sequences grouped in two classes regarding their genomic organization: Tandem repeats and dispersed repeats. In tandem repeats, copies of a short DNA sequence are positioned one after another within the genome, while in dispersed repeats, these copies are randomly distributed. In this review we provide evidence that both tandem and dispersed repeats can have a similar organization, which leads us to suggest an update to their classification based on the sequence features, concretely regarding the presence or absence of retrotransposons/transposon specific domains. In addition, we analyze several studies that show that a repetitive element can be remodeled into repetitive non-coding or coding sequences, suggesting (1) an evolutionary relationship among DNA sequences, and (2) that the evolution of the genomes involved frequent repetitive sequence reshuffling, a process that we have designated as a “DNA remodeling mechanism”. The alternative classification of the repetitive DNA sequences here proposed will provide a novel theoretical framework that recognizes the importance of DNA remodeling for the evolution and plasticity of eukaryotic genomes.This work was financially supported by a Newfelpro Post-doctoral grant (No. 82) from Republic of Croatia co-financed through the Marie Curie FP7-PEOPLE-2011-COFUND program

Epigenetic regulation of cdh1 is altered after hoxb7-silencing in mda-mb-468 triple-negative breast cancer cells

HOXB7 is often overexpressed in breast cancer cells and found to relate to poor prognosis. The search for the HOXB7 targets, as a transcription factor, has led to molecules involved in regulating cell proliferation, migration, invasion, and processes such as angiogenesis and therapy resistance. However, the specific targets affected by the deregulation of HOXB7 in breast cancer remain largely unknown in most molecular sub-types, such as triple-negative breast cancers (TNBC). To unveil the molecular basis behind these aggressive and often untreatable cancers, here we explored the contribution of HOXB7 deregulation for their aggressiveness. To this end, HOXB7 was silenced in TNBC Basal A cells MDA-MB-468, and the phenotype, gene/protein expression, and methylation profile of putative targets were analyzed. Lower migration and invasion rates were detected in HOXB7-silenced cells in comparison with the controls. In addition, these cells expressed more CDH1 and less DNMT3B, and the promoter methylation status of CDH1 diminished. Our data suggest that the HOXB7 transcription factor may act on TNBC Basal A cells by controlling CDH1 epigenetic regulation. This may occur indirectly through the up-regulation of DNMT3B, which then controls DNA methylation of the CDH1 promoter. Thus, future approaches interfering with HOXB7 regulation may be promising therapeutic strategies in TNBC treatment.This research was funded by FEDER (Fundo Europeu de Desenvolvimento Regional) funds through the COMPETE 2020 Operational Programme for Competitiveness and Internationalisation (POCI-01-0145-FEDER-030562), Portugal 2020, and by Portuguese funds through FCT (Fundação para a Ciência e a Tecnologia/Ministério da Ciência, Tecnologia e Ensino Superior) in the framework of the project PTDC/BTM-TEC/30562/2017

Closed flow solar dehydration with the use of silver nanoparticles: Application for the production of Pouteria lucuma flour

In this research, a closed-flow solar dehydrator with a refrigeration moisture extraction system was evaluated, likewise, the dehydration temperature time was optimized by evaluating three types of heat transfer fluids. The dehydration equipment included devices to absorb thermal energy from incident sunlight, such as a trombe wall and a parabolic cylindrical collector, and a thermo bank system. In addition, the influence of three types of heat transfer fluids (water, oil and oil nanofluidþsilver nanoparticles) was evaluated. This dehydration system was applied to process the Pouteria lucuma fruit. The results indicate the reduction of the dehydration time by 58.19% using nanofluid. This treatment prevents the modification of the physicochemical properties of the product and helps preserving its organoleptic propertiesinfo:eu-repo/semantics/publishedVersio

Digital health literacy in the training of informal caregivers - community intervention

Background: Health Literacy allows optimizing healthy lifestyles and preventive and health protective behaviors (DGS, 2019). Low literacy can lead to a greater number of hospitalizations, a more frequent use of emergency services and a lower prevalence of preventive attitudes in the field of health. Internet-based interventions could have a positive impact on informal caregivers, reducing the geographical barrier, promoting self-efficacy in managing their own emotions, reducing burden. Aim: Contribute to the training of informal caregivers of a primary health care unit in Lisbon, through the promotion of digital health literacy. Methods: The Community Intervention project was carried out in the context of a home visit, focusing on 11 informal caregivers, through the presentation of an interactive digital manual. It was based on the methodology of health planning, through the elaboration of a diagnosis of the situation, definition of priorities, setting of objectives, selection of strategies, operational preparation and evaluation (Imperatori & Giraldes, 1993).ResultsIt was found that not all informal caregivers have access to the internet or digital technologies, and it was necessary to deliver the printed manual. Caregivers who accessed the interactive digital manual rated its content as very important, having accessed the suggested links without difficulty. The possibility of forwarding the digital manual to other caregivers was valid for all. Conclusions:Digital technologies promote communication in terms of health promotion, contributing to universal access and digital training in health, giving individuals the opportunity to increase care for their own health. The creation of digital health tools must be directed to the characteristics of the population. For individuals with low digital literacy, simple technologies must be created and for those who cannot or do not want to use digital tools, adequate alternatives must be created.info:eu-repo/semantics/publishedVersio

Does gender really matter when we are talking about energy saving attitudes and behaviours?

Some studies indicate that women tend to be more pro-environmental than men, since they buy more green products, play a more active role in the separation of packages for recycling and display behaviour that is more favourable in regard to saving energy resources. With a specific focus on gender differences, this study aims to compare English and Portuguese students’ attitudes and behaviours in relation to energy saving. Three universities participated in this study, one from Portugal (PT) and two from the United Kingdom (UK), with data collected through a questionnaire. The results point to a number of significant differences in relation to gender between the respondents from the two countries, and some consistent trends in gender differences across the whole sample and the sub-samples from the different countries. The key trends identified overall are that female students are significantly more likely to express positive attitudes towards energy saving, and to undertake energy-saving behaviours. Differences between the sub-samples are relatively small compared to the similarities between them, suggesting that gender differences are reasonably consistent across the two countries

Evaluating the engagement of universities in capacity building for sustainable development in local communities

Universities have the potential to play a leading role in enabling communities to develop more sustainable ways of living and working however, sustainable communities may only emerge with facilitation, community learning and continual efforts to build their capacities. Elements of programme planning and evaluation on the one hand, and capacity building on the other, are needed. The latter entails approaches and processes that may contribute to community empowerment; universities may either lead such approaches, or be key partners in an endeavour to empower communities to address the challenges posed by the need for sustainable development. Although capacity building and the promotion of sustainable development locally, are on the agenda for universities who take seriously regional engagement, very little is published that illustrates or describes the various forms of activities that take place. Further, there is a paucity of studies that have evaluated the work performed by universities in building capacity for sustainable development at the local level. This paper is an attempt to address this need, and entails an empirical study based on a sample of universities in the United Kingdom, Germany, Portugal and Brazil. The paper examines the extent to which capacity building for sustainable development is being undertaken, suggests the forms that this might take and evaluates some of the benefits for local communities. The paper concludes by reinforcing that universities have a critical role to play in community development; that role has to prioritise the sustainability agenda

An analysis of the measurement of the construct “buying behaviour” in green marketing

The process behind people´s decisions to buy green products is a complex one, and the factors that influence purchase behaviours are still not fully understood. This paper is an attempt to contribute to the state of knowledge in this field, by providing an analysis of the measurement of the construct buying behaviour in green marketing. In particular, it reports on the results of a study involving a sample of 1175 university students from four countries using a survey approach to test a green behavioural model. The method of data collection took the form of a self-administered questionnaire. The results gathered in the research have shown that the Straughan and Roberts’ scale of green buying behaviour studied presents a high level of reliability, above other commonly used scales and is a useful measure of green buying behaviour; some of the findings may help to improve the effectiveness of green marketing

Deficit Irrigation in Mediterranean Fruit Trees and Grapevines: Water Stress Indicators and Crop Responses

In regions with Mediterranean climate, water is the major environmental resource that limits growth and production of plants, experiencing a long period of water scarcity during summer. Despite the fact that most plants developed morphological, anatomical, physiological, and biochemical mechanisms that allow to cope with such environments, these harsh summer conditions reduce growth, yield, and fruit quality. Irrigation is implemented to overcome such effects. Conditions of mild water deficit imposed by deficit irrigation strategies, with minimal effects on yield, are particularly suitable for such regions. Efficient irrigation strategies and scheduling techniques require the quantification of crop water requirements but also the identification of pertinent water stress indicators and their threshold. This chapter reviews the scientific information about deficit irrigation recommendations and thresholds concerning water stress indicators on peach trees, olive trees, and grapevines, as case studies

Roles of the hox proteins in cancer invasion and metastasis

Invasion and metastasis correspond to the foremost cause of cancer-related death, and the molecular networks behind these two processes are extremely complex and dependent on the intra-and extracellular conditions along with the prime of the premetastatic niche. Currently, several studies suggest an association between the levels of HOX genes expression and cancer cell invasion and metastasis, which favour the formation of novel tumour masses. The deregulation of HOX genes by HMGA2/TET1 signalling and the regulatory effect of noncoding RNAs generated by the HOX loci can also promote invasion and metastasis, interfering with the expression of HOX genes or other genes relevant to these processes. In this review, we present five molecular mechanisms of HOX deregulation by which the HOX clusters products may affect invasion and metastatic processes in solid tumours.The work was financed by FEDER (Fundo Europeu de Desenvolvimento Regional) funds through the COMPETE 2020 Operational Programme for Competitiveness and Internationalisa-tion (POCI), Portugal 2020, and by Portuguese funds through FCT (Fundação para a Ciência e a Tecnologia/Ministério da Ciência, Tecnologia e Ensino Superior) in the framework of the project POCI-01-0145-FEDER-030562 (PTDC/BTM-TEC/30562/2017). The authors have no other relevant affiliations or financial involvement with any organization or entity with a financial interest in or financial conflict with the subject matter or materials discussed in the manuscript apart from those disclosed

Role of QseG membrane protein in beneficial enterobacterial interactions with plants and Mesorhizobia

Membrane protein Quorum sensing G (QseG) positively interferes in the process of colonization and infection of enteric pathogens in animals. Its gene is located between qseE and qseF genes and is co-transcribed with the two-component system. Homologs of qseG gene, along with qseEF, are present in many Enterobacteriaceae; however, its role in nonpathogenic strains is still unknown. To fill this knowledge gap, we investigated the role of QseG protein of a plant-associated enterobacterium in the interactions with its legume host and in the benefits induced by this enterobacterium in the Mesorhizobium-chickpea symbiosis. Here, we show that Kosakonia sp. MH5 ΔqseG mutant was defective in internal root colonization and inoculation of chickpea seedlings with this mutant increased the expression of the defence-related gene CaRBOH-like in host roots. Furthermore, we show that invasion and a proper establishment within the roots and/or root nodules are essential for MH5 strain to be able to exert beneficial effects on the symbiotic Mesorhizobium-chickpea association under salinity. This study demonstrates, for the first time, that the role of QseG is transversal to pathogenic and nonpathogenic enterobacteria and is a step forward to better understanding the molecular bases of plant-bacteria interactions established between legume and beneficial endophytic enterobacteria