16 research outputs found
The future of layer-by-layer assembly: A tribute to ACS Nano associate editor Helmuth Möhwald
Layer-by-layer (LbL) assembly is a widely used tool for engineering materials and coatings. In this Perspective, dedicated to the memory of ACS Nano associate editor Prof. Dr. Helmuth Möhwald, we discuss the developments and applications that are to come in LbL assembly, focusing on coatings, bulk materials, membranes, nanocomposites, and delivery vehicles
The meanings of cultural competence in mental health: an exploratory focus group study with patients, clinicians, and administrators
Child Injury Risks are Close to Home: Parent Psychosocial Factors Associated with Child Safety
Imaging spectroscopy links aspen genotype with below-ground processes at landscape scales
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The 15th Anniversary of the U.S. National Nanotechnology Initiative.
Recommended from our members
The 15th Anniversary of the U.S. National Nanotechnology Initiative.
Local ATP generation by brain-type creatine kinase (CK-B) facilitates cell motility
Contains fulltext :
76049.pdf (publisher's version ) (Open Access)BACKGROUND: Creatine Kinases (CK) catalyze the reversible transfer of high-energy phosphate groups between ATP and phosphocreatine, thereby playing a storage and distribution role in cellular energetics. Brain-type CK (CK-B) deficiency is coupled to loss of function in neural cell circuits, altered bone-remodeling by osteoclasts and complement-mediated phagocytotic activity of macrophages, processes sharing dependency on actomyosin dynamics. METHODOLOGY/PRINCIPAL FINDINGS: Here, we provide evidence for direct coupling between CK-B and actomyosin activities in cortical microdomains of astrocytes and fibroblasts during spreading and migration. CK-B transiently accumulates in membrane ruffles and ablation of CK-B activity affects spreading and migration performance. Complementation experiments in CK-B-deficient fibroblasts, using new strategies to force protein relocalization from cytosol to cortical sites at membranes, confirmed the contribution of compartmentalized CK-B to cell morphogenetic dynamics. CONCLUSION/SIGNIFICANCE: Our results provide evidence that local cytoskeletal dynamics during cell motility is coupled to on-site availability of ATP generated by CK-B