Nuclear actin and myosins in adenovirus infection

Adenovirus serotypes have been shown to cause drastic changes in nuclear organization, including the transcription machinery, during infection. This ability of adenovirus to subvert transcription in the host cell facilitates viral replication. Because nuclear actin and nuclear myosin I, myosin V and myosin VI have been implicated as direct regulators of transcription and important factors in the replication of other viruses, we sought to determine how nuclear actin and myosins are involved in adenovirus infection. We first confirmed reorganization of the host's transcription machinery to viral replication centers. We found that nuclear actin also reorganizes to sites of transcription through the intermediate but not the advanced late phase of viral infection. Furthermore, nuclear myosin I localized with nuclear actin and sites of transcription in viral replication centers. Intriguingly, nuclear myosins V and VI, which also reorganized to viral replication centers, exhibited different localization patterns, suggesting specialized roles for these nuclear myosins. Finally, we assessed the role of actin in adenovirus infection and found both cytoplasmic and nuclear actin likely play roles in adenovirus infection and replication. Together our data suggest the involvement of actin and multiple myosins in the nuclear replication and late viral gene expression of adenovirus.Fil: Fuchsova, Beata. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Biotecnológicas. Instituto de Investigaciones Biotecnológicas ; Argentina. University of Illinois; Estados UnidosFil: Serebryannyy, Leonid A.. University of Illinois; Estados UnidosFil: De Lanerolle, Primal. University of Illinois; Estados Unido

Android Permissions Remystified: A Field Study on Contextual Integrity

Due to the amount of data that smartphone applications can potentially access, platforms enforce permission systems that allow users to regulate how applications access protected resources. If users are asked to make security decisions too frequently and in benign situations, they may become habituated and approve all future requests without regard for the consequences. If they are asked to make too few security decisions, they may become concerned that the platform is revealing too much sensitive information. To explore this tradeoff, we instrumented the Android platform to collect data regarding how often and under what circumstances smartphone applications are accessing protected resources regulated by permissions. We performed a 36-person field study to explore the notion of "contextual integrity," that is, how often are applications accessing protected resources when users are not expecting it? Based on our collection of 27 million data points and exit interviews with participants, we examine the situations in which users would like the ability to deny applications access to protected resources. We found out that at least 80% of our participants would have preferred to prevent at least one permission request, and overall, they thought that over a third of requests were invasive and desired a mechanism to block them

Hypnobirthing Pain Management for Maternal Pain Adaptation along Labor Process

The purpose of this study was to determine the effect of hypnobirthing pain management on the adaptability of maternal pain spontaneously. This study uses a quantitative approach to the design quasi-experimental design with treatment replications ( pretest posttest with control group.  Research results showed the existence difference in pain adaptation in mothers who are given hypnobirthing pain management intervention is 1,00 (able to adapt). No differences in pain adaptation in women who were not given pain management interventions hipnobirthing ie -0.62 (not able to adapt). Bivariate results revealed the influence of hypnobirthing pain management on the adaptation ability of maternal pain that gave birth spontaneously (p=0.002). We conclude that there is an influence of hypnobirthing pain management on pain adaptability. It is expected that medical services are able to apply hypnobirthing pain management to maternity mothers, and expected to make standardized services procedures on hypnobirthing management for pain relaxations.    Keywords : Hypnobirthing, Pain Adaptation, Pain Managemen

Guided Imagery Technique Implementation Reducing Primigravida Pregnancy Anxiety Before Childbirth Delivery

Anxiety is the most common psychiatric condition found throughout the world. Anxiety in pregnancy is an emotional reaction that occurs in pregnant women related to the concern of the mother with the welfare of herself and her fetus. Guided imagery is the development of mental functions that express oneself dynamically through a psychophysiological process involving all senses and bringing about changes in behavior, perception, or physiological responses with someone's guidance or through the media. This scientific case study aims to determine the application of nursing care to primigravida mothers in dealing with the labor process using guided imagery techniques. The data collection techniques used were interviews, observation, physical examination, and documentation study. This scientific paper shows that there is a decrease in the level of anxiety in the patient after doing the guided imagery technique for 3 meetings. Based on these results, it can be concluded that the intervention from journals related to nursing care in primigravida mothers who experience anxiety has been implemented well. The results of this scientific case study are expected to be implemented as a basis for developing nursing care management and assisting nurses to increase client’s satisfaction with caring provided. Keywords: anxiety, guided imagery, primigravida pregnancy

Mothers Awareness of Pneumonia and the Toddler Breastfeeding Duration Affect Pneumonia Incidence

This study aims to determine the relationship between the mother's level of awareness about the pathology of pneumonia and the duration of breastfeeding under five with the incidence of pneumonia in DR. M.A. Hospital Hanafiah. This research is a retrospective analytic descriptive study with a cross-sectional design. The results showed that mothers' awareness of pneumonia was lower by 71.8%, they breastfed their babies for less than six months (71.8%), and more than two-thirds of children under five (76.9%) were diagnosed with pneumonia. Furthermore, statistical analysis significantly correlated the mothers' awareness of pneumonia and the duration of breastfeeding they performed on their children with pneumonia. The conclusion is that the mother's understanding of pneumonia has a linear effect on the incidence of pneumonia in infants and toddlers. The duration of breastfeeding has a significant impact. We assume that non-exclusive breastfeeding in infants stimulates underweight and immunological disorders that accelerate the incidence of pneumonia.   Keywords: Mother's Awareness, Length of Breastfeeding, Toddler Pneumoni

The Feasibility of Dynamically Granted Permissions: Aligning Mobile Privacy with User Preferences

Current smartphone operating systems regulate application permissions by prompting users on an ask-on-first-use basis. Prior research has shown that this method is ineffective because it fails to account for context: the circumstances under which an application first requests access to data may be vastly different than the circumstances under which it subsequently requests access. We performed a longitudinal 131-person field study to analyze the contextuality behind user privacy decisions to regulate access to sensitive resources. We built a classifier to make privacy decisions on the user's behalf by detecting when context has changed and, when necessary, inferring privacy preferences based on the user's past decisions and behavior. Our goal is to automatically grant appropriate resource requests without further user intervention, deny inappropriate requests, and only prompt the user when the system is uncertain of the user's preferences. We show that our approach can accurately predict users' privacy decisions 96.8% of the time, which is a four-fold reduction in error rate compared to current systems.Comment: 17 pages, 4 figure

Nuclear actin: to polymerize or not to polymerize

The form and function of actin in the nucleus have been enigmatic for over 30 years. Recently actin has been assigned numerous functional roles in the nucleus, but its form remains a mystery. The intricate relationship between actin form and function in the cytoplasm implies that understanding the structural properties of nuclear actin is elementary to fully understanding its function. In this issue, McDonald et al. (p. 541) use fluorescence recovery after photobleaching (FRAP) to tackle the question of whether nuclear actin exists as monomers or polymers

PENGARUH PES PLANUS PLANTARIS METATARSALIA TERHADAP STABILITAS POSTURAL VERTEBRA ATLET OLAHRAGA DENGAN WEIGHT-BEARING

Overuse activity is at risk of injury to the lower limb and will decrease curvature in the plantar arch. Sports athletes with continuous weight-bearing exercise activity will affect the curvature of the plantar, causing the malformation of the foot arch to become flatter (flatfoot). This condition is reported to affect postural stability during the move. This is a quantitative cross-sectional analytics study involving basketball athletes for all 47 subjects. Subject's foot arch index determined with footprint on the graph paper, followed by AMTI Accupower Force flatform posturography (force plate) as postural stability determintion. The examination is performed in static positition to determine body,s postural sway diameter of CoP on the base of support area while standing. The result revealed that 80,9% of subjects had flat plantar arch (pes planus plantaris). Footprint results showed majorities of basketball athletes having pes planus plantaris. The mean value of the postural sway diameter of CoP while standing (static) on the subject with pes planus plantaris have significant correlation on the postural stability. This condition will significantly affect postural stability in quite standing conditions