Antioxidant and Antimicrobial Activities of Salvia multicaulis

The present study aimed to determine antioxidant and antimicrobial activities of Salvia multicaulis Vahl plant collected in Gaziantep province, Turkey. Total antioxidant status (TAS), total oxidant status (TOS) and oxidative stress index (OSI) were determined using Rel Assay kits. Antimicrobial activity was determined with modified agar dilution method. The findings demonstrated that S. multicaulis had high antioxidant activity. However, it is recommended to avoid excessive consumption of the plant due to high OSI. It was also determined that plant extracts possessed antimicrobial potential. As a result, it was determined that the plant can be used as a natural antioxidant and antimicrobial resource

Hidrofobik Yüzeylerin Türbülanslı Boru Akımlarında Sürtünme Kayıplarına Etkisinin Deneysel İncelenmesi

Bu çalışmada, pürüzsüz bakır boru iç yüzeyi hidrofobik özellik kazandırılacak şekilde kaplanarak türbülanslı akımda basınç kaybı ve sürtünme faktörü üzerindeki etkisi deneysel olarak incelenmiştir. Kaplama malzemesi olarak floroidetilenpropilen (FEP) ve bu temel malzemeye ağırlıkça %1 oranda grafen (FEP-G) ve grafit (FEP-C) ekleyerek elde edilen solüsyonlar kullanılmıştır. FEP, FEP-G ve FEP-C malzemeleri ile pürüzsüz bakır boru iç yüzeyleri sprey yöntemi ile kaplanıp 400 °C sıcaklıkta kurutularak hidrofobik yüzeyler elde edilmiştir. İşlenmemiş konvansiyonel pürüzsüz bakır yüzeyi ile FEP, FEP-C ve FEP-G kaplı yüzeylerin hidrofobikliği temas açıları sırasıyla 65°, 93°, 96° ve 102° olarak ölçülerek belirlenmiştir. Türbülanslı akımda, 5000 – 30000 Reynolds sayıları aralığında deneysel olarak basınç kayıpları ölçülerek sürtünme faktörü belirlenmiştir. FEP, FEP-C ve FEP-G kaplı hidrofobik yüzeyler, konvansiyonel bakır yüzeye kıyasla 5000 – 30000 Reynolds sayısı aralığındaki türbülanslı akımda %7 - %36.1 aralığında sürtünme faktöründe bir azalma göstermiştir

Proteomic analysis of flowers at two developmental stages in Thermopsis turcica (Fabaceae)

Flower development is a complex physiological phenomenon that is affected by environmental stimuli and endogenous signals. Several molecular approaches have provided a comprehensive view of the physiological processes associated with flower development, but little is known about proteomic changes. Therefore, we investigated the proteomic alterations during flower development in endemic Thermopsis turcica (Vuralia turcica), an unusual legume species with 3–4 free carpels in a single flower. A comparative proteomic analysis to identify proteins involved in flower development was carried out in T. turcica. Alterations in proteomes of the flower buds and fully opened flowers were studied by two-dimensional gel electrophoresis. A total of 66 proteins were differentially expressed during flower development. Among them, 32 protein spots were identified by mass spectrometry. Functional annotation of these flower proteins revealed their involvement in transcription and protein metabolism, energy and carbohydrate metabolisms, plant defense, cell walls, photosynthesis, secondary metabolism, and amino acid metabolism. Moreover, most of these proteins were downregulated at the later stage of flower development. Proteins involved in protein metabolism, sugar metabolism, and stress defense are regulated during flower development, suggesting that they have possible roles in developmental regulation. These results have given new insights into the proteome alterations during flower development

High Gain Ultra-Wideband Parabolic Reflector Antenna Design Using Printed LPDA Antenna Feed

Reflector antennas with log periodic dipole array (LPDA) feeds are ideal for applications that demand high gain, broadband operation. However, when the phase center of the LPDA is not fixed, mismatches at the focal point cause degradation and large ripple in gain. To overcome these issues, a printed LPDA is optimized for minimal phase center variation as a reflector antenna feed. The antenna is designed to operate at 1-19 GHz frequency band with voltage standing wave ratio (VSWR) less than 3.0 and minimum gain of 17 dBi. Reflector size can be increased for further improvement in gain. Designed antenna parameters, radiation patterns, and aperture efficiencies over frequencies are presented and compared to previous studies.

Effect of cytokine genes in the pathogenesis and on the clinical parameters for the treatment of multiple myeloma

WOS: 000394527000002PubMed ID: 27611810In this study, we aimed to explore the association among gene variants of five cytokines, tumor necrosis factor alpha (TNF-alpha), transforming growth factor beta-1 (TGF-beta 1), interferon gamma (IFN-gamma), interleukin-6 (IL-6), and interleukin-10 (IL-10), and clinical parameters and prognosis in patients with multiple myeloma (MM) treated with novel therapeutic drugs in Turkish population for the first time except TNF-alpha. We analyzed five cytokine genes in 113 cases with MM and 113 healthy controls. Cytokine genotyping was performed by the polymerase chain reaction-sequence-specific primer method (PCR-SSP). AG genotype associated with high expression in TNF-alpha gene (-308) variant was found to be significantly higher (p = 0.019), and GG genotype associated with low expression in TNF-alpha gene (-308) variant was significantly lower in MM group as compared with controls (p = 0.012). IFN-gamma (+874) variant TT genotype was increased (p = 0.037), and AA genotype was decreased (p = 0.002) in MM group in contrast to controls. IFN-gamma (+874) T allele was higher inMMpatients compared with controls (OR = 1.985, p = 0.000), while A allele was significantly lower (OR = 0.5037, p = 0.0005). Multivariate analysis revealed that factors associated with 5-year overall survival (OS) were only IPI III (RR = 1.630, p = 0.018) and thrombocytopenia (RR = 2.207, Cox p = 0.021), while 5-year event-free survival (EFS) was associated with IPI III (RR = 1.524, p = 0.022), thrombocytopenia (RR = 2.902, p = 0.002), APSCT treatment (RR = 1.729, p = 0.035), and female gender (RR = 0.435, p = 0.002) with negative prognostic values. Our results suggested that TNF-alpha gene (-308) AG genotype and IFN-gamma (+874) TT genotype and T allele may have a role on MM, while other cytokines were not associated with the risk of MM

Medical Biology and Genetics Laboratory Book

80 Pages : İllustrations ; 26 cm

Antioxidant and antimicrobial activities of ethanol extract of Helianthemum salicifolium (Cistaceae)

Plants have been used by humans to treat diseases. Different plant species have been very successful in treating different diseases. Helianthemum salicifolium (L.) Mill. was used as a material in our study. The plant was extracted with ethanol (EtOH) in a Soxhlet apparatus. Then, the antioxidant (TAS) and oxidant status (TOS) of the plant extract were determined using Rel Assay kits. Their antimicrobial activities were tested against standard bacteria and fungus strains by the agar dilution method. As a result of the analysis, the TAS value of plant extract was determined as 9.490±0.195, TOS value as 14.839±0.253, and OSI value as 0.157±0.005. In this context, it was seen that the plant has important antioxidant potential. In addition, the plant extract was found to be effective against test microorganisms at 25-100 μg/mL extract concentrations. Also, the extract was found to be more effective against fungus strains (C. albicans, C. Glabrata, and C. krusei). As a result, it was determined that H. salicifolium could be a natural antioxidant and antimicrobial source

Antioxidant and antimicrobial activities of ethanol extract of Helianthemum salicifolium (Cistaceae)

459-462Plants have been used by humans to treat diseases. Different plant species have been very successful in treating different diseases. Helianthemum salicifolium (L.) Mill. was used as a material in our study. The plant was extracted with ethanol (EtOH) in a Soxhlet apparatus. Then, the antioxidant (TAS) and oxidant status (TOS) of the plant extract were determined using Rel Assay kits. Their antimicrobial activities were tested against standard bacteria and fungus strains by the agar dilution method. As a result of the analysis, the TAS value of plant extract was determined as 9.490±0.195, TOS value as 14.839±0.253, and OSI value as 0.157±0.005. In this context, it was seen that the plant has important antioxidant potential. In addition, the plant extract was found to be effective against test microorganisms at 25-100 μg/mL extract concentrations. Also, the extract was found to be more effective against fungus strains (C. albicans, C. Glabrata, and C.krusei). As a result, it was determined that H. salicifolium could be a natural antioxidant and antimicrobial source

XRCC4 rs6869366 polymorphism is associated with susceptibility to both nicotine dependence and/or schizophrenia

Background: Oxidative stress induced DNA damage has been assumed to contribute to the etiopathogenesis of schizophrenia (Sch). Smoking prevalence was more common in patients with Sch. The X-ray repair cross-complementation group 4 (XRCC4) gene plays an important role in the repair of DNA double-strand breaks. Objective: The purpose of this study was to investigate whether XRCC4 rs6869366 polymorphism has a relationship both in nicotine dependence (ND) and Sch+ND risk. Methods: One hundred and four patients with Sch+ND, 133 subjects with ND only and 70 healthy controls were enrolled in the study. XRCC4 rs6869366 polymorphism was analyzed using PCR-RFLP assay. Results: The frequency of XRCC4 rs6869366 GG genotype was more common in the ND and Sch+ND group than controls (p = 0.001 and p = 0.001, respectively). XRCC4 rs6869366 TT genotype was lower in both ND and Sch+ND group compared to controls (p = 0.001 and p = 0.001, respectively). Also, XRCC4 rs6869366 G allele was higher in Sch+ND group than controls (p = 0.001) while XRCC4 rs6869366 T allele was lower in ND group than healthy controls (p=0.001). XRCC4 rs6869366 GT genotype was lower in ND group than control group (p = 0.003). Discussion: These results suggested that the XRCC4 rs6869366 polymorphism G related genotype/allele was associated with susceptibility to both ND and Sch+ND in a Turkish population