Training in Honey Bee Veterinary Medicine in Italy: An Observational Study and Practical Proposals to Face Professional Challenges

Honey bees, like other livestock, may be affected by infectious, parasitic, and abiotic diseases that need proper sanitary monitoring and control. Currently, there are limited opportunities for undergraduate students to receive education in Honey Bee Veterinary Medicine (HBVM) as part of their regular degree program, despite the professional requirements for veterinarians to carry out the increasing tasks related to honey bee health and production. Additionally, postgraduate training and specialization in HBVM is also underdeveloped. This study was an observational survey that evaluated the educational opportunities available in HBVM for current and future veterinarians in Italy. The survey analyzed both undergraduate and postgraduate programs, including Undergraduate Degree Programs in Veterinary Medicine (UDPVM), “Scuole di Specializzazione”, Masters, and other postgraduate courses. The results indicate that the current training available for veterinarians in the field of apiculture, both before and after graduation, is also insufficient in Italy, as already reported in other EU- and extra-EU countries. Finally, a roadmap for veterinary training in HBVM is developed here describing objectives and teachings aimed at fulfilling the needs of the profession in the field of beekeeping, considering the existing rules and regulations governing public health and possible evolution of this legal framework in the futur

Detection of honey bee viruses in larvae of Vespa orientalis

: The Oriental hornet (Vespa orientalis) is one of the major predators of honey bees. It has been demonstrated that adults of V. orientalis can harbor honey bee viruses, however the transmission route of infection is still not clear. The aim of this study was to study the possible presence of honey bee viruses in V. orientalis larvae and honey bees collected from the same apiary. Therefore, 29 samples of V. orientalis larvae and 2 pools of honey bee (Apis mellifera). samples were analyzed by multiplex PCR to detect the presence of six honeybee viruses: Acute Bee Paralysis Virus (ABPV), Black Queen Cell Virus (BQCV), Chronic Bee Paralysis Virus (CBPV), Deformed Wing Virus (DWV), Kashmir Bee Virus (KBV) and Sac Brood Virus (SBV). Biomolecular analysis of V. orientalis larvae revealed that DWV was present in 24/29 samples, SBV in 10/29, BQCV in 7/29 samples and ABPV in 5/29 samples, while no sample was found positive for CBPV or KBV. From biomolecular analysis of honey bee samples DWV was the most detected virus, followed by SBV, BQCV, ABPV. No honey bee sample was found positive for CBPV or KBV. Considering the overlapping of positivities between V.orientalis larvae and honey bee samples, and that V.orientalis larvae are fed insect proteins, preferably honey bees, we can suggest the acquisition of viral particles through the ingestion of infected bees. However, future studies are needed to confirm this hypothesis and rule out any other source of infection

Expression of vascular endothelial growth factor (VEGF) in equine sarcoid

Background: Sarcoids are the mostcommon skin tumors in horses, ch aracterized by rare regression, invasiveness and high recurrence following surgical intervention and Delta Papillomaviruses are widely recognized as the causative agents of the disease. In order to gain new insights into equine sarco id development, we have evaluated, in 25 equine sarcoids, by immunohistochemistry and western blotting analysis, the expression levels of VEGF, Ki67 and bcl-2. Moreover, we have measured microvessel density an d specific vessel parameters. Results: All sarcoid samples showed a strong and finely granular cytoplasmatic staining for VEGF in the majority (90%) of keratinocytes, sarcoid fibroblasts and endothelial cells. Numerous small blood vessels, immunostained with Von Willebrand factor, often appeared irregular in shape and without a distinct lumen, with mean values of microvessel area and perimeter lower than normal. Moreover, in all sarcoid samples, Ki67 immunoreactivity was moderately positive in 5 – 10% of dermal sarcoid fibroblasts, while Bcl2 immunoreactivity was detected in 52% of the sarcoid samples, with a weak staining in 20 – 50% of dermal sarcoid fibroblasts. Biochemical analysis was consistent with immunohistochemical results. Conclusions: This study has provided evidence that in equine sarcoid: VEGF was strongly expressed; the increased number of vessels was not associated with their complete maturation, probably leading to a hypoxic condition, which could increase VEGF synthesis; the levels of sarcoid fibroblasts proliferation were very low. Concluding, VEGF may have a role in equine sarcoid development, not only through the increase of angiogenesis, but also through the control of sarcoid fibroblast activity

Discovery of the heavily obscured supernova 2002cv

On the 13th of May 2002, supernova 2002cv was discovered using a near-infrared camera working at the AZT-24 1.1m telescope at Campo Imperatore (AQ-Italy). After the infrared detection a simultaneous photometric follow-up was started at optical wavelengths. The preliminary results confirm a heavily obscured object with a V-K color not lower than 6 magnitudes, making SN 2002cv the most reddened supernova ever observed. This finding, along with the recent discovery of another obscured supernova, suggests a critical revision of the rates known to date. The estimate of the visual extinction and the light curves are provided here. These latter indicate that our SN 2002cv observations are the earliest available for a type-Ia supernova at IR wavelengths.Comment: 4 page

The effect of radiation pressure on virial black hole mass estimates and the case of Narrow Line Seyfert 1 galaxies

We consider the effect of radiation pressure from ionizing photons on black hole (BH) mass estimates based on the application of the virial theorem to broad emission lines in AGN spectra. BH masses based only on the virial product V^2R and neglecting the effect of radiation pressure can be severely underestimated especially in objects close to the Eddington limit. We provide an empirical calibration of the correction for radiation pressure and we show that it is consistent with a simple physical model in which BLR clouds are optically thick to ionizing radiation and have average column densities of NH~10^23 cm^-2. This value is remarkably similar to what is required in standard BLR photoionization models to explain observed spectra. With the inclusion of radiation pressure the discrepancy between virial BH masses based on single epoch spectra and on reverberation mapping data drops from 0.4 to 0.2 dex rms. The use of single epoch observations as surrogates of reverberation mapping campaigns can thus provide more accurate BH masses than previously thought. Finally, we show that Narrow Line Seyfert 1 (NLS1) galaxies have apparently low BH masses because they are radiating close to their Eddington limit. After the radiation pressure correction, NLS1 galaxies have BH masses similar to other broad line AGNs and follow the same MBH-sigma/L relations as other active and normal galaxies. Radiation forces arising from ionizing photon momentum deposition constitute an important physical effect which must be taken into account when computing virial BH masses.Comment: To appear in the Astrophysical Journal, May 1 issu

Expression pattern of estroprogestinic receptors in sinonasal inverted papilloma

open13openSerra A; Caltabiano R; Spinato G; Gallina S; Caruso S; Rapisarda V; Di Mauro P; Castro V; Conti A; Licciardello L; Maiolino L; Lanzafame S; Cocuzza SSerra, A; Caltabiano, R; Spinato, G; Gallina, S; Caruso, S; Rapisarda, V; Di Mauro, P; Castro, V; Conti, A; Licciardello, L; Maiolino, L; Lanzafame, S; Cocuzza,

Kiloparsec-scale gaseous clumps and star formation at z = 5–7

We investigate the morphology of the [Cii] emission in a sample of “normal” star-forming galaxies at 5 < z < 7:2 in relation to their UV (rest-frame) counterpart. We use new ALMA observations of galaxies at z 6 7, as well as a careful re-analysis of archival ALMA data. In total 29 galaxies were analysed, 21 of which are detected in [Cii]. For several of the latter the [Cii] emission breaks into multiple components. Only a fraction of these [Cii] components, if any, is associated with the primary UV systems, while the bulk of the [Cii] emission is associated either with fainter UV components, or not associated with any UV counterpart at the current limits. By taking into account the presence of all these components, we find that the L[CII]-SFR relation at early epochs is fully consistent with the local relation, but it has a dispersion of 0.48 0.07 dex, which is about two times larger than observed locally. We also find that the deviation from the local L[CII]-SFR relation has a weak anti-correlation with the EW(Ly ). The morphological analysis also reveals that [Cii] emission is generally much more extended than the UV emission. As a consequence, these primordial galaxies are characterised by a [Cii] surface brightness generally much lower than expected from the local [CII] SFR relation. These properties are likely a consequence of a combination of di erent e ects, namely: gas metallicity, [Cii] emission from obscured star-forming regions, strong variations of the ionisation parameter, and circumgalactic gas in accretion or ejected by these primeval galaxies.European Research Council RM acknowledges ERC Advanced Grant 695671 ‘QUENCH’. AF acknowledges support from the ERC Advanced Grant INTERSTELLAR H2020/740120

On the Evolution of the Star Formation Rate Function of Massive Galaxies. Constraints at 0.4<z<1.8 from the GOODS-MUSIC Catalogue

[abridged] We study the evolution of the Star Formation Rate Function (SFRF) of massive galaxies over the 0.4<z<1.8 redshift range and its implications for our understanding of the physical processes responsible for galaxy evolution. We use multiwavelength observations included in the GOODS-MUSIC catalogue, which provides a suitable coverage of the spectral region from 0.3 to 24 micron and either spectroscopic or photometric redshifts for each object. Individual SFRs have been obtained by combining UV and 24 micron observations, when the latter were available. For all other sources an "SED fitting" SFR estimate has been considered. We then define a stellar mass limited sample, complete in the Mstar>1.e10 Msun range and determine the SFRF using the 1/Vmax algorithm. We define simulated galaxy catalogues based on three different semi-analytical models of galaxy formation and evolution. We show that the theoretical SFRFs are well described by a double power law functional form and its redshift evolution is approximated with high accuracy by a pure evolution of the typical SFR. We find good agreement between model predictions and the high-SFR end of the SFRF, when the observational errors on the SFR are taken into account. However, the observational SFRF is characterised by a double peaked structure, which is absent in its theoretical counterparts. At z>1.0 the observed SFRF shows a relevant density evolution, which is not reproduced by SAMs, due to the well known overprediction of intermediate mass galaxies at z~2. The agreement at the low-SFR end is poor: all models overpredict the space density of SFR~1 Msun/yr and no model reproduces the double peaked shape of the observational SFRF. If confirmed by deeper IR observations, this discrepancy will provide a key constraint on theoretical modelling of star formation and stellar feedback.Comment: 12 pages, 4 figures and 3 table. Accepted for publication by MNRAS - updated reference