Self-interested service-oriented agents based on trust and QoS for dynamic reconfiguration

Progressively increasing complexity of dynamic environments, in which services and applications are demanded by potential clients, requires a high level of reconfiguration of the offer to better match that ever changing demand. In particular, the dynamic change of the client’s needs, leading to higher exigency, may require a smart and flexible automatic composition of more elementary services. By leveraging the service-oriented architectures and multi-agent system benefits, the paper proposes a method to explore the flexibility of the decision support for the services’ reconfiguration based on several pillars, such as trust, reputation and QoS models, which allows the selection based on measuring the expected performance of the agents. Preliminary experimental results, extracted from a real case scenario, allow highlighting the benefits of the proposed distributed and flexible solution to balance the workload of service providers in a simple and fast manner. The proposed solution includes the agents’ intelligent decision-making capability to dynamically and autonomously change services selection on the fly, towards more trustworthy services with better quality when unexpected events happen, e.g. broken machines. We then propose the use of competitive self-interested agents to provide services that best suits to the client through dynamic service composition.info:eu-repo/semantics/publishedVersio

A investigação criminal pelo Ministério Público, à luz da teoria de dos direitos fundamentais de Robert Alexy: estudo do HC 91.613-MG/STF

O presente trabalho se propõe a analisar a constitucionalidade do caráter subsidiário das investigações criminais realizadas pelo Ministério Público enquanto nova tendência de consolidação de jurisprudência do Supremo Tribunal Federal Brasileiro, vazada no HC 91.613/MG. Propõe-se, no presente trabalho, uma revisão da mencionada decisão pretoriana sob um olhar mais criterioso, especificamente sob a bandeira de um controle racional da decisão judicial, devendo-se utilizar para tanto os estudos do renomado doutrinador Robert Alexy sobre a matéria. Nossa pretensão não é propriamente pesquisar o que significa este caráter subsidiário das investigações, mas sim, investigar se a construção da decisão que levou a estabelecer esse modelo de investigação passa pelo crivo da racionalidade ou é mais um exemplo de mero decisionismo judicial, enquanto aspecto negativo do chamado “ativismo judicial”. Palavras-chave: investigação criminal; Ministério Público; subsidiariedade; racionalidade; ponderação; sopesamento; igualdade. CRIMINAL PROSECUTION OF PUBLIC MINISTRY AT THE LIGHT OF ROBERT ALEXY´S THEORY OF FUNDAMENTAL RIGHTS: THE CASE HC - 91 613 MG / STF ABSTRACT This paper aims to examine the constitutionality of the subsidiary nature of criminal investigations by prosecutors as new Brazilian Supreme Court jurisprudence consolidation trend, poured into the HC 91,613 / MG. It is proposed in this paper, a review of that decision under praetorian a more careful look, specifically under the banner of a rational control of the court decision, should be used for both studies the renowned theoretician Robert Alexy on the matter. Our intention is not really research what this means subsidiary nature of the investigations, but rather investigate whether the construction of the decision that led to establish this research model passes the test of rationality or is it more a case of mere judicial decisionism, while negative the so-called "judicial activism". KEY WORDS: Data de submissão: 17/11/2015 Data de aceitação: 18/02/201

Therapeutic Breast Reconstruction Using Gene Therapy–Delivered IFNγ Immunotherapy

After mastectomy, breast reconstruction is increasingly performed using autologous tissue with the aim of improving quality of life. During this procedure, autologous tissue is excised, relocated, and reattached using vascular anastomoses at the site of the extirpated breast. The period during which the tissue is ex vivo may allow genetic modification without any systemic exposure to the vector. Could such access be used to deliver therapeutic agents using the tissue flap as a vehicle? Such delivery may be more efficient than systemic treatment, in terms of oncological outcomes. The cytokine interferon gamma (IFNγ) has antitumor effects, but systemic toxicity that could be circumvented if its effect can be localized by delivery of the IFNγ gene via gene therapy to autologous tissue used for breast reconstruction, which then releases IFNγ and exerts anti-tumor effects. In a rat model of loco-regional recurrence (LRR) using both MADB-106-Luc and MAD-MB-231-Luc breast cancer cells, autologous tissue was transduced ex vivo with an adeno-associated viral vector (AAV) encoding IFNγ. The therapeutic reconstruction released IFNγ at the LRR site and eliminated cancer cells, significantly decreased tumor burden (P<0.05), and increased survival by 33% (P<0.05) compared to sham reconstruction. Mechanistically, localized IFNγ immunotherapy stimulated M1 macrophages to target cancer cells within the regional confines of the modified tumor environment. This concept of therapeutic breast reconstruction using ex vivo gene therapy of autologous tissue offers a new application for immunotherapy in breast cancer with a dual therapeutic effect of both reconstructing the ablative defect and delivering local adjuvant immunotherapy

Inter-rater reliability of physiotherapists using the Action Research Arm Test in chronic stroke

The purpose of this study is to establish whether physiotherapists' ratings are consistent, when using the Action Research Arm Test (ARAT) to score a chronic stroke patient. This was part of a large project establishing the reliability in chronic stroke. This study used a correlational design comparing the association between physiotherapist scores of the same patient, to establish the ARAT's inter-rater reliability. The COSMIN checklist was followed to enhance the methodology of the study. Twenty physiotherapists (8 female and 12 male) aged between 25 and 53 years were selected. There were no participant dropouts or withdrawals. The sample size was normally distributed. The physiotherapists appeared representative of the UK physiotherapy population, with the exception of gender. The distribution of scores showed a normal distribution with standard deviation of score of 1.9. The Kendall's W test showed 0.711 of agreement between the raters. The scores achieved statistical significance showing consistency between physiotherapists' scores with chronic stroke. Limitations of the study were the use of a small single center convenience sample that may reduce the generalizability of the findings. The ARAT is consistent when scored by physiotherapists in a chronic stroke population. The inter-rater reliability range was (0.70 to 0.90) which is categorized as good

Accounting Problems Under the Excess Profits Tax

DNA vaccines based on subunits from pathogens have several advantages over other vaccine strategies. DNA vaccines can easily be modified, they show good safety profiles, are stable and inexpensive to produce, and the immune response can be focused to the antigen of interest. However, the immunogenicity of DNA vaccines which is generally quite low needs to be improved. Electroporation and co-delivery of genetically encoded immune adjuvants are two strategies aiming at increasing the efficacy of DNA vaccines. Here, we have examined whether targeting to antigen-presenting cells (APC) could increase the immune response to surface envelope glycoprotein (Env) gp120 from Human Immunodeficiency Virus type 1 (HIV- 1). To target APC, we utilized a homodimeric vaccine format denoted vaccibody, which enables covalent fusion of gp120 to molecules that can target APC. Two molecules were tested for their efficiency as targeting units: the antibody-derived single chain Fragment variable (scFv) specific for the major histocompatilibility complex (MHC) class II I-E molecules, and the CC chemokine ligand 3 (CCL3). The vaccines were delivered as DNA into muscle of mice with or without electroporation. Targeting of gp120 to MHC class II molecules induced antibodies that neutralized HIV-1 and that persisted for more than a year after one single immunization with electroporation. Targeting by CCL3 significantly increased the number of HIV-1 gp120-reactive CD8(+) T cells compared to non-targeted vaccines and gp120 delivered alone in the absence of electroporation. The data suggest that chemokines are promising molecular adjuvants because small amounts can attract immune cells and promote immune responses without advanced equipment such as electroporation.Funding Agencies|Research Council of Norway; Odd Fellow</p

Genotoxicity biomonitoring of sewage in two municipal wastewater treatment plants using the Tradescantia pallida var. purpurea bioassay

The genotoxicity of untreated and treated sewage from two municipal wastewater treatment plants (WTP BN and WTP SJN) in the municipality of Porto Alegre, in the southern Brazilian state of Rio Grande do Sul, was evaluated over a one-year period using the Tradescantia pallida var. purpurea (Trad-MCN) bioassay. Inflorescences of T. pallida var. purpurea were exposed to sewage samples in February (summer), April (autumn), July (winter) and October (spring) 2009, and the micronuclei (MCN) frequencies were estimated in each period. The high genotoxicity of untreated sewage from WTP BN in February and April was not observed in treated sewage, indicating the efficiency of treatment at this WTP. However, untreated and treated sewage samples from WTP SJN had high MCN frequencies, except in October, when rainfall may have been responsible for reducing these frequencies at both WTPs. Physicochemical analyses of sewage from both WTPs indicated elevated concentrations of organic matter that were higher at WTP SJN than at WTP BN. Chromium was detected in untreated and treated sewage from WTP SJN, but not in treated sewage from WTP BN. Lead was found in all untreated sewage samples from WTP SJN, but only in the summer and autumn at WTP BN. These results indicate that the short-term Trad-MCN genotoxicity assay may be useful for regular monitoring of municipal WTPs

Direct Measurement of Nuclear Dependence of Charged Current Quasielastic-like Neutrino Interactions using MINERvA

Charged-current $\nu_{\mu}$ interactions on carbon, iron, and lead with a final state hadronic system of one or more protons with zero mesons are used to investigate the influence of the nuclear environment on quasielastic-like interactions. The transfered four-momentum squared to the target nucleus, $Q^2$, is reconstructed based on the kinematics of the leading proton, and differential cross sections versus $Q^2$ and the cross-section ratios of iron, lead and carbon to scintillator are measured for the first time in a single experiment. The measurements show a dependence on atomic number. While the quasielastic-like scattering on carbon is compatible with predictions, the trends exhibited by scattering on iron and lead favor a prediction with intranuclear rescattering of hadrons accounted for by a conventional particle cascade treatment. These measurements help discriminate between different models of both initial state nucleons and final state interactions used in the neutrino oscillation experiments