Between-day Reliability of Pedal Forces for Cyclists During an Incremental Cycling Test to Exhaustion

BACKGROUND: Reliability of pedal forces during cycling specific tests needs to be established if pedal forces are to be used in longitudinal studies evaluating performance improvements. METHOD: We assessed the reliability of pedal force measures during two incremental cycling tests to exhaustion separated by two to seven days. The number of competitive cyclists completing each workload increment varied (n=10 for 100 W to 250 W; n=8 for 300 W; n=6 for 350 W). Pedal forces were measured via strain gauge instrumented pedals and pedal-to-crank angles via angular potentiometers attached to the pedal spindles. Mean and standard deviations, typical error of measurement percentage (TE%) and effect sizes (ES) between days across workloads for oxygen uptake (VO_{2}), peak normal force (PNF), peak anterior-posterior force (PAPF), average total force on the pedal (ATF) and index of effectiveness percentage (IE) for right and left pedals were calculated. RESULTS: Averages across all workloads showed high reliability and trivial differences between two to seven days of testing for all variables (TE%, ICC, ES; VO_{2}=4%, 0.94, 0.1; PNFright=6%, 0.98, 0.1; PNFleft=12%, 0.98, 0.1; PAPFright=13%, 0.95, 0.2; PAPFleft=14%, 0.96, 0.1; ATFright=5%, 0.98, 0.1; ATFleft=11%, 0.97, 0.1; IEright=10%, 0.94, 0.2; IEleft=14%, 0.91, 0.1). CONCLUSION: Pedal force measures during incremental cycling tests to exhaustion can be used to assess changes in performance given the high reliability reported in our study

Using rowers’ perceptions of on-water stroke success to evaluate sculling catch efficiency variables via a boat instrumentation system

Aim: An effective catch in sculling is a critical determinant of boat velocity. This study used rowers’ performance-based judgments to compare three measures of catch slip efficiency. Two questions were addressed: (1) would rower-judged Yes strokes be faster than No strokes? and (2) which method of quantifying catch slip best reflected these judgements? Methods: Eight single scullers performed two 10-min blocks of sub maximal on-water rowing at 20 strokes per minute. Every 30 s, rowers reported either Yes or No about the quality of their stroke at the catch. Results: It was found that Yes strokes identified by rowers had, on average, a moderate effect advantage over No strokes with a standardised effect size of 0.43. In addition, a quicker time to positive acceleration best reflected the change in performance; where the standardised mean difference score of 0.57 for time to positive acceleration was larger than the scores of 0.47 for time to PowerLine force, and 0.35 for time to 30% peak pin force catch slip measures. For all eight rowers, Yes strokes corresponded to time to positive acceleration occurring earlier than No strokes. Conclusion: Rower judgements about successful strokes was linked to achieving a quicker time to positive acceleration, and may be of the most value in achieving a higher average boat velocity

Recreational snow-sports injury risk factors and countermeasures: a meta-analysis review and Haddon Matrix Evaluation

Background: Snow sports (alpine skiing/snowboarding) would benefit from easily implemented and cost-effective injury prevention countermeasures that are effective in reducing injury rate and severity. Objective: For snow sports, to identify risk factors and to quantify evidence for effectiveness of injury prevention countermeasures. Methods: Searches of electronic literature databases to February 2014 identified 98 articles focused on snow sports that met the inclusion criteria and were subsequently reviewed. Pooled odds ratios (ORs) with 90 % confidence intervals (CIs) and inferences (percentage likelihood of benefit/harm) were calculated using data from 55 studies using a spreadsheet for combining independent groups with a weighting factor based on quality rating scores for effects. Results: More experienced skiers and snowboarders are more likely to sustain an injury as a result of jumps, while beginners sustain injuries primarily as a result of falls. Key risk factors that countermeasure interventions should focus on include, beginner skiers (OR 2.72; 90 % CI 2.15–3.44, 99 % most likely harmful), beginner snowboarders (OR 2.66; 90 % CI 2.08–3.40, 99 % harmful), skiers/snowboarders who rent snow equipment (OR 2.58; 90 % CI 1.98–3.37, 99 % harmful) and poor visibility due to inclement weather (OR 2.69; 90 % CI 1.43–5.07, 97 % harmful). Effective countermeasures include helmets for skiers/snowboarders to prevent head injuries (OR 0.58; 90 % CI 0.51–0.66, 99 % most likely beneficial), and wrist guards for snowboarders to prevent wrist injuries (OR 0.33; 90 % CI 0.23–0.47, 99 % beneficial). Discussion: The review identified key risk factors for snow-sport injuries and evaluated the evidence for the effectiveness of existing injury prevention countermeasures in recreational (general public use of slopes, not racing) snow sports using a Haddon’s matrix conceptual framework for injury causation (host/snow-sport participant, agent/mechanism and environment/community). Conclusion: Best evidence for the effectiveness of injury prevention countermeasures in recreational snow sports was for the use of helmets and wrist guards and to address low visibility issues via weather reports and signage

Effects of saddle height on pedal force effectiveness

Bicycle saddle height configuration may affect pedal force application. Our aim was to compare pedal force effectiveness for different saddle height configurations. Eleven cyclists (38 ± 12 years) and eleven triathletes (44 ± 8 years) with competitive experience performed 2-min trials at four different saddle heights (preferred, high, low, theoretical optimal) each separated by one minute of rest. Workload was normalized by body weight and pedalling cadence was visually controlled by the athletes at 90 ± 2 rpm for all trials. The preferred saddle height replicated the horizontal and vertical configuration of each athlete’s bicycle. High and low saddle heights were selected to elicit ± 10° knee flexion from knee flexion at preferred saddle height. Guidelines from Peveler were used to set the theoretical optimal saddle height based on 25° knee flexion when the pedal crank was at the 6 o’clock position. Knee joint angles were measured with a goniometer prior to each trial. Normal and shear forces were measured using an instrumented right pedal and pedal-to-crank angle was measured using an angular potentiometer. A reed switch attached to the bicycle frame detected the position of the crank in relation to the pedal revolution. Forces on the pedal surface were resolved into the tangential force on the crank to compute force effectiveness (ratio between tangential and resultant force applied on the pedal). Magnitudes of differences between the saddle heights were assessed by effect sizes (ES) for the average total (resultant) force and force effectiveness. To elicit ± 10° knee flexion, changes of ± 3% of the preferred saddle height were required. Changes in average resultant force with saddle height were trivial (1% for preferred versus optimal; ES = 0.2) to moderate (5% for high versus low; ES = 0.8). Changes in force effectiveness with saddle height were small (2% for preferred versus optimal; ES = 0.3) to moderate (6% for high versus low; ES = 1.0). Lower saddle heights produced higher resultant force but lower force effectiveness. Saddle height changes resulted in moderate effects for pedal resultant force and force effectiveness for most saddle height comparisons

Molecular crowding defines a common origin for the Warburg effect in proliferating cells and the lactate threshold in muscle physiology

Aerobic glycolysis is a seemingly wasteful mode of ATP production that is seen both in rapidly proliferating mammalian cells and highly active contracting muscles, but whether there is a common origin for its presence in these widely different systems is unknown. To study this issue, here we develop a model of human central metabolism that incorporates a solvent capacity constraint of metabolic enzymes and mitochondria, accounting for their occupied volume densities, while assuming glucose and/or fatty acid utilization. The model demonstrates that activation of aerobic glycolysis is favored above a threshold metabolic rate in both rapidly proliferating cells and heavily contracting muscles, because it provides higher ATP yield per volume density than mitochondrial oxidative phosphorylation. In the case of muscle physiology, the model also predicts that before the lactate switch, fatty acid oxidation increases, reaches a maximum, and then decreases to zero with concomitant increase in glucose utilization, in agreement with the empirical evidence. These results are further corroborated by a larger scale model, including biosynthesis of major cell biomass components. The larger scale model also predicts that in proliferating cells the lactate switch is accompanied by activation of glutaminolysis, another distinctive feature of the Warburg effect. In conclusion, intracellular molecular crowding is a fundamental constraint for cell metabolism in both rapidly proliferating- and non-proliferating cells with high metabolic demand. Addition of this constraint to metabolic flux balance models can explain several observations of mammalian cell metabolism under steady state conditions

Serratamolide is a hemolytic factor produced by Serratia marcescens

Serratia marcescens is a common contaminant of contact lens cases and lenses. Hemolytic factors of S. marcescens contribute to the virulence of this opportunistic bacterial pathogen. We took advantage of an observed hyper-hemolytic phenotype of crp mutants to investigate mechanisms of hemolysis. A genetic screen revealed that swrW is necessary for the hyper-hemolysis phenotype of crp mutants. The swrW gene is required for biosynthesis of the biosurfactant serratamolide, previously shown to be a broad-spectrum antibiotic and to contribute to swarming motility. Multicopy expression of swrW or mutation of the hexS transcription factor gene, a known inhibitor of swrW expression, led to an increase in hemolysis. Surfactant zones and expression from an swrW-transcriptional reporter were elevated in a crp mutant compared to the wild type. Purified serratamolide was hemolytic to sheep and murine red blood cells and cytotoxic to human airway and corneal limbal epithelial cells in vitro. The swrW gene was found in the majority of contact lens isolates tested. Genetic and biochemical analysis implicate the biosurfactant serratamolide as a hemolysin. This novel hemolysin may contribute to irritation and infections associated with contact lens use. © 2012 Shanks et al

Auxetic foam for snowsport safety devices

Skiing and snowboarding are popular snow-sports with inherent risk of injury. There is potential to reduce the prevalence of injuries by improving and implementing snow-sport safety devices with the application of advanced materials. This paper investigates the application of auxetic foam to snow-sport safety devices. Composite pads - consisting of foam covered with a semi-rigid shell - were investigated as a simple model of body armour and a large 70 x 355 x 355 mm auxetic foam sample was fabricated as an example crash barrier. The thermo-mechanical conversion process was applied to convert open-cell polyurethane foam to auxetic foam. The composite pad with auxetic foam absorbed around three times more energy than the conventional equivalent under quasi-static compression with a concentrated load, indicating potential for body armour applications. An adapted thermo-mechanical process - utilising through-thickness rods to control in-plane compression - was applied to fabricate the large sample with relatively consistent properties throughout, indicating further potential for fabrication of a full size auxetic crash barrier. Further work will create full size prototypes of snow-sport safety devices with comparative testing against current products

ECG Response of Koalas to Tourists Proximity: A Preliminary Study

Koalas operate on a tight energy budget and, thus, may not always display behavioral avoidance reaction when placed in a stressful condition. We investigated the physiological response of captive koalas Phascolarctos cinereus in a conservation centre to the presence of tourists walking through their habitat. We compared, using animal-attached data-recorders, the electrocardiogram activity of female koalas in contact with tourists and in a human-free area. One of the koalas in the tourist zone presented elevated heart rate values and variability throughout the recording period. The remaining female in the exhibit area showed a higher field resting heart rates during the daytime than that in the isolated area. In the evening, heart rate profiles changed drastically and both the koalas in the exhibit and in the tourist-free zones displayed similar field resting heart rates, which were lower than those during the day. In parallel, the autonomic nervous systems of these two individuals evolved from sympathetic-dominant during the day to parasympathetic-dominant in the evening. Our results report ECG of free-living koalas for the first time. Although they are preliminary due to the difficulty of having sufficient samples of animals of the same sex and age, our results stress out the importance of studies investigating the physiological reaction of animals to tourists