The VLT-FLAMES Tarantula Survey

A spectroscopic analysis has been undertaken for the B-type multiple systems (excluding those with supergiant primaries) in the VLT-FLAMES Tarantula Survey (VFTS). Projected rotational velocities, vesini, for the primaries have been estimated using a Fourier Transform technique and confirmed by fitting rotationally broadened profiles. A subset of 33 systems with vesini ≤ 80 km s-1 have been analysed using a TLUSTY grid of model atmospheres to estimate stellar parameters and surface abundances for the primaries. The effects of a potential flux contribution from an unseen secondary have also been considered. For 20 targets it was possible to reliably estimate their effective temperatures (Teff) but for the other 13 objects it was only possible to provide a constraint of 20 000 ≤ Teff ≤ 26 000 K – the other parameters estimated for these targets will be consequently less reliable. The estimated stellar properties are compared with evolutionary models and are generally consistent with their membership of 30 Doradus, while the nature of the secondaries of 3 SB2 system is discussed. A comparison with a sample of single stars with vesini ≤ 80 km s-1 obtained from the VFTS and analysed with the same techniques implies that the atmospheric parameters and nitrogen abundances of the two samples are similar. However, the binary sample may have a lack of primaries with significant nitrogen enhancements, which would be consistent with them having low rotational velocities and having effectively evolved as single stars without significant rotational mixing. This result, which may be actually a consequence of the limitations of the pathfinder investigation presented in this paper, should be considered as a motivation for spectroscopic abundance analysis of large samples of binary stars, with high quality observational data

The VLT-FLAMES Tarantula Survey XXVIII. Nitrogen abundances for apparently single dwarf and giant B-type stars with small projected rotational velocities

Previous analyses of the spectra of OB-type stars in the Magellanic Clouds have identified targets with low projected rotational velocities and relatively high nitrogen abundances; the evolutionary status of these objects remains unclear. The VLT-FLAMES Tarantula Survey obtained spectroscopy for over 800 early-type stars in 30 Doradus of which 434 stars were classified as B-type. We have estimated atmospheric parameters and nitrogen abundances using tlusty model atmospheres for 54 B-type targets that appear to be single, have projected rotational velocities, ve sin i ≤ 80 km s−1 and were not classified as supergiants. In addition, nitrogen abundances for 34 similar stars observed in a previous FLAMES survey of the Large Magellanic Cloud have been re-evaluated. For both samples, approximately 75-80% of the targets have nitrogen enhancements of less than 0.3 dex, consistent with them having experienced only small amounts of mixing. However, stars with low projected rotational velocities, ve sin i ≤ 40 km s−1 and significant nitrogen enrichments are found in both our samples and simulations imply that these cannot all be rapidly rotating objects observed near pole-on. For example, adopting an enhancement threshold of 0.6 dex, we observed five and four stars in our VFTS and previous FLAMES survey samples, yet stellar evolution models with rotation predict only 1.25±1.11 and 0.26±0.51 based on our sample sizes and random stellar viewing inclinations. The excess of such objects is estimated to be 20-30% of all stars with current rotational velocities of less than 40 km s−1 . This would correspond to ∼2-4% of the total non-supergiant single B-type sample. Given the relatively large nitrogen enhancement adopted, these estimates constitute lower limits for stars that appear inconsistent with current grids of stellar evolutionary models. Including targets with smaller nitrogen enhancements of greater than 0.2 dex implies larger percentages of targets that are inconsistent with current evolutionary models, viz. ∼70% of the stars with rotational velocities less than 40 km s−1 and ∼6-8% of the total single stellar population. We consider possible explanations of which the most promising would appear to be breaking due to magnetic fields or stellar mergers with subsequent magnetic braking

Identification of nine new susceptibility loci for endometrial cancer

Endometrial cancer is the most commonly diagnosed cancer of the female reproductive tract in developed countries. Through genome-wide association studies (GWAS), we have previously identified eight risk loci for endometrial cancer. Here, we present an expanded meta-analysis of 12,906 endometrial cancer cases and 108,979 controls (including new genotype data for 5624 cases) and identify nine novel genome-wide significant loci, including a locus on 12q24.12 previously identified by meta-GWAS of endometrial and colorectal cancer. At five loci, expression quantitative trait locus (eQTL) analyses identify candidate causal genes; risk alleles at two of these loci associate with decreased expression of genes, which encode negative regulators of oncogenic signal transduction proteins (SH2B3 (12q24.12) and NF1 (17q11.2)). In summary, this study has doubled the number of known endometrial cancer risk loci and revealed candidate causal genes for future study

SARS-CoV-2-specific nasal IgA wanes 9 months after hospitalisation with COVID-19 and is not induced by subsequent vaccination

BACKGROUND: Most studies of immunity to SARS-CoV-2 focus on circulating antibody, giving limited insights into mucosal defences that prevent viral replication and onward transmission. We studied nasal and plasma antibody responses one year after hospitalisation for COVID-19, including a period when SARS-CoV-2 vaccination was introduced. METHODS: In this follow up study, plasma and nasosorption samples were prospectively collected from 446 adults hospitalised for COVID-19 between February 2020 and March 2021 via the ISARIC4C and PHOSP-COVID consortia. IgA and IgG responses to NP and S of ancestral SARS-CoV-2, Delta and Omicron (BA.1) variants were measured by electrochemiluminescence and compared with plasma neutralisation data. FINDINGS: Strong and consistent nasal anti-NP and anti-S IgA responses were demonstrated, which remained elevated for nine months (p < 0.0001). Nasal and plasma anti-S IgG remained elevated for at least 12 months (p < 0.0001) with plasma neutralising titres that were raised against all variants compared to controls (p < 0.0001). Of 323 with complete data, 307 were vaccinated between 6 and 12 months; coinciding with rises in nasal and plasma IgA and IgG anti-S titres for all SARS-CoV-2 variants, although the change in nasal IgA was minimal (1.46-fold change after 10 months, p = 0.011) and the median remained below the positive threshold determined by pre-pandemic controls. Samples 12 months after admission showed no association between nasal IgA and plasma IgG anti-S responses (R = 0.05, p = 0.18), indicating that nasal IgA responses are distinct from those in plasma and minimally boosted by vaccination. INTERPRETATION: The decline in nasal IgA responses 9 months after infection and minimal impact of subsequent vaccination may explain the lack of long-lasting nasal defence against reinfection and the limited effects of vaccination on transmission. These findings highlight the need to develop vaccines that enhance nasal immunity. FUNDING: This study has been supported by ISARIC4C and PHOSP-COVID consortia. ISARIC4C is supported by grants from the National Institute for Health and Care Research and the Medical Research Council. Liverpool Experimental Cancer Medicine Centre provided infrastructure support for this research. The PHOSP-COVD study is jointly funded by UK Research and Innovation and National Institute of Health and Care Research. The funders were not involved in the study design, interpretation of data or the writing of this manuscript