Evaluation of Ganoderma lucidum strains for the production of bioactive components and their potential use as antimicrobial agents

In the present study, mycelial biomass of Ganoderma lucidum strains was produced in mushroom complete media under optimized culture conditions of temperature, pH, agitation and the fruiting bodies were raised on wheat straw supplemented with 5 per cent rice bran. Maximum biomass (16.39 mg ml-1) was recorded in strain GL-3 and minimum (10.99 mg ml-1) was observed in strain GL-1 after 28 days of incubation period. Maximum biological efficiency of 13.1 per cent was obtained in 57 days in GL-2 strain. Moreover, weight of fruiting bodies was highest (91 g bag-1) in 5 per cent wheat bran supplemented bags in strain GL-2. Polysaccharides were extracted, characterized and analysed. The maximum yield of polysaccharides was obtained from fruiting bodies (30.06- 63.70%), followed by mycelial biomass (7.61- 21.32%) and culture filtrates (0.86- 2.86%). Non-reducing sugars were the main fraction of sugars which constituted 69.82- 70.39 per cent in fruiting body extracted polysaccharides, 62.08- 83.39 per cent in biomass and 59.75- 77.54 per cent in crude extracts. The extracts from fruiting bodies and biomass of G. lucidum strains also exhibited antibacterial effect against some pathogenic bacteria, Staphylococcus sp. and Enterobacter sp. The present study clearly indicated that variations existed among G. lucidum strains with respect to the production of bioactive molecules, yield and antimicrobial activity. Bioactive components from G. lucidum strains are promising antimicrobial agents that can be harnessed as potent antimicrobial toxicant

Selenium uptake and associated anti-oxidant properties in Pleurotus fossulatus cultivated on wheat straw from seleniferous fields

The present study was carried out to examine the antioxidant activity in oyster mushroom cultivated on selenium (Se) rich substrate. Pleurotus fossulatus was cultivated on Se-rich wheat straw collected from the seleniferous belt of Punjab (India) and its potential to accumulate Se from substrate was examined. Using different assay systems the modulations in the anti-oxidant profile of Se enriched mushroom was studied in comparison to the mushrooms cultivated on normal straw. The oyster mushrooms were observed to potentially mobilize Se from Se-rich substrates to fruiting bodies, resulting in significantly high uptake (37.2±0.6 μg g−1) as compared to control (3.57±0.53 mg g−1). The antioxidant activity, as determined by various assays, such as reducing power, 2,2-diphenyl-1-picrylhydrazyl free radical scavenging, and metal chelating activity, was higher in the experimental mushrooms when compared to control. The results obtained demonstrate that Se-fortified mushrooms through cultivation on straw containing organic forms of Se can be considered as natural and effective dietary supplements of organic Se for humans. The present study proposes the use of Se-rich agricultural residues as substrates for mushroom cultivation for human and livestock supplementation

Transient stability enhancement of a gridconnected wind farm using an adaptive neurofuzzy controlled-flywheel energy storage system

With the rapid growth of the wind energy systems in the past years and their interconnection with the existing power system networks, it has become very significant to analyse and enhance the transient stability of the wind energy conversion systems connected to the grid. This study investigates the transient stability enhancement of a grid-connected wind farm using doubly-fed induction machine-based flywheel energy storage system. A cascaded adaptive neuro-fuzzy controller (ANFC) is introduced to control the insulated gate bipolar transistor switches-based frequency converter to enhance the transient stability of the grid-connected wind farm. The performance of the proposed control strategy is analysed under a severe symmetrical fault condition on both a single-machine infinite bus model and the IEEE-39 bus New England test system. The transient performance of the system is investigated by comparing the results of the system using the proposed ANFCs with that of the black-box optimisation technique-based proportional-integral controllers. The validity of the system is verified by the simulation results which are carried out using PSCAD/EMTDC environment

Nutrition, atherosclerosis, arterial imaging, cardiovascular risk stratification, and manifestations in COVID-19 framework: a narrative review.

Background: Atherosclerosis is the primary cause of the cardiovascular disease (CVD). Several risk factors lead to atherosclerosis, and altered nutrition is one among those. Nutrition has been ignored quite often in the process of CVD risk assessment. Altered nutrition along with carotid ultrasound imaging-driven atherosclerotic plaque features can help in understanding and banishing the problems associated with the late diagnosis of CVD. Artificial intelligence (AI) is another promisingly adopted technology for CVD risk assessment and management. Therefore, we hypothesize that the risk of atherosclerotic CVD can be accurately monitored using carotid ultrasound imaging, predicted using AI-based algorithms, and reduced with the help of proper nutrition. Layout: The review presents a pathophysiological link between nutrition and atherosclerosis by gaining a deep insight into the processes involved at each stage of plaque development. After targeting the causes and finding out results by low-cost, user-friendly, ultrasound-based arterial imaging, it is important to (i) stratify the risks and (ii) monitor them by measuring plaque burden and computing risk score as part of the preventive framework. Artificial intelligence (AI)-based strategies are used to provide efficient CVD risk assessments. Finally, the review presents the role of AI for CVD risk assessment during COVID-19. Conclusions: By studying the mechanism of low-density lipoprotein formation, saturated and trans fat, and other dietary components that lead to plaque formation, we demonstrate the use of CVD risk assessment due to nutrition and atherosclerosis disease formation during normal and COVID times. Further, nutrition if included, as a part of the associated risk factors can benefit from atherosclerotic disease progression and its management using AI-based CVD risk assessment

Expert consensus statements for the management of COVID-19-related acute respiratory failure using a Delphi method.

Coronavirus disease 2019 (COVID-19) pandemic has caused unprecedented pressure on healthcare system globally. Lack of high-quality evidence on the respiratory management of COVID-19-related acute respiratory failure (C-ARF) has resulted in wide variation in clinical practice. Using a Delphi process, an international panel of 39 experts developed clinical practice statements on the respiratory management of C-ARF in areas where evidence is absent or limited. Agreement was defined as achieved when > 70% experts voted for a given option on the Likert scale statement or > 80% voted for a particular option in multiple-choice questions. Stability was assessed between the two concluding rounds for each statement, using the non-parametric Chi-square (χ <sup>2</sup> ) test (p < 0·05 was considered as unstable). Agreement was achieved for 27 (73%) management strategies which were then used to develop expert clinical practice statements. Experts agreed that COVID-19-related acute respiratory distress syndrome (ARDS) is clinically similar to other forms of ARDS. The Delphi process yielded strong suggestions for use of systemic corticosteroids for critical COVID-19; awake self-proning to improve oxygenation and high flow nasal oxygen to potentially reduce tracheal intubation; non-invasive ventilation for patients with mixed hypoxemic-hypercapnic respiratory failure; tracheal intubation for poor mentation, hemodynamic instability or severe hypoxemia; closed suction systems; lung protective ventilation; prone ventilation (for 16-24 h per day) to improve oxygenation; neuromuscular blocking agents for patient-ventilator dyssynchrony; avoiding delay in extubation for the risk of reintubation; and similar timing of tracheostomy as in non-COVID-19 patients. There was no agreement on positive end expiratory pressure titration or the choice of personal protective equipment. Using a Delphi method, an agreement among experts was reached for 27 statements from which 20 expert clinical practice statements were derived on the respiratory management of C-ARF, addressing important decisions for patient management in areas where evidence is either absent or limited. The study was registered with Clinical trials.gov Identifier: NCT04534569