Microbial Production Of Propionic And Succinic Acid From Sorbitol Using Propionibacterium Acidipropionici

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)Three sequential fermentative batches were carried out with cell recycle in four simultaneously operating bioreactors maintained at pH 6.5, 30 degrees C, and 100 rpm. P. acidipropionici ATCC 4875 was able to produce propionic and succinic acid from sorbitol. The concentration of propionic acid decreased slightly from 39.5 +/- 5.2 g L-1 to 34.4 +/- 1.9 g L-1, and that of succinic acid increased significantly from 6.1 +/- 2.1 g L-1 to 14.8 +/- 0.9 g L-1 through the sequential batches. In addition, a small amount of acetic acid was produced that decreased from 3.3 +/- 0.4 g L-1 to 2.0 +/- 0.3 g L-1 through the batches. The major yield for propionic acid was 0.613 g g(-1) in the first batch and succinic acid it was 0.212 g g(-1) in the third batch. The minor yield of acetic acid was 0.029 g g(-1), in the second and third batches.5Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)BRASKEM S/AFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)FAPESP [2011/51861-9

What are the motives underlying Brazilians' food choices? An analysis of the Food Choice Questionnaire and its relationship with different sample characteristics

ABSTRACT: The first aim of this study was to evaluate the psychometric properties of the Food Choice Questionnaire (FCQ)—using the original model—in a sample of 1480 Brazilian adults (69.5% female). The second aim was to rank the reasons underlying the participants' food choices using average FCQ scores and 95% confidence interval. The third aim was to evaluate the relationship between food choice motives and sample characteristics using multiple logistic regression and odds ratios. The validity, the invariance across different groups, and the reliability of the FCQ were confirmed for the sample. Sensory appeal and price emerged as the most important reasons, while ethical concern was the least valued. The factors associated with greater odds of choosing food for specific reasons were being older, female, and a student; practicing physical activity; dieting frequently; self-rating eating quality as good; having a higher body mass index; and having low income. Practical Applications Assessing food choice is a complex task, as it encompasses several factors, such as sensory characteristics, health status, income, culture, lifestyle, and cognitive-affective issues; therefore, the use of appropriate tools should be encouraged. The set analyses followed confirmed that the FCQ was an adequate instrument to evaluate the reasons for food choice of the participants who valued strongly the sensory aspects of the foods and presented specific characteristics (e.g., diet practice) that may influence their decisions. These findings may guide future research and clinical interventions aimed at producing food choices that are more oriented to health and well-being.FAPESPinfo:eu-repo/semantics/publishedVersio

A qualitative study of the spiritual aspects of parenting a child with Down syndrome

Parenting a child with Down syndrome can sometimes present certain difficulties and, thus, spirituality may function as a dimension related to finding meaning in life and as a coping resource. Spirituality is a critical dimension of nursing care, but scarce knowledge is available to specifically inform family nursing practice. The aim of this study was to explore the spiritual aspects of parenting a child with Down syndrome, as a qualitative secondary analysis. This is an observational qualitative study, based on in-depth interviews from 42 participants. Data analysis found seven categories that concern meaning and purpose in life: hope, family strength, spiritual practices, personal beliefs, and love, and trust in healthcare providers. Spirituality is a resource in parents' lives who are living in this situation. Nurses should consider this dimension in supporting families and in improving management of this life and health condition.info:eu-repo/semantics/publishedVersio

A Portuguese Adaptation of the Teruel Orthorexia Scale and a Test of Its Utility with Brazilian Young Adults

The aims for this study were to perform a Portuguese language cross-cultural adaptation of the Teruel Orthorexia Scale (TOS) and to evaluate the scale's psychometric properties, including verifying the frequency of behaviors characteristic of orthorexia nervosa and healthy orthorexia, among a group of Brazilian gym users. First, we adapted the Spanish version of the TOS to the Brazilian Portuguese language following international protocols to guarantee idiomatic, semantic, conceptual, and cultural equivalence. Then participants completed both the new Portuguese version of the TOS and a socioeconomic questionnaire. Among our sample of 226 young Brazilian adults (63.7% men; M age = 28.8, SD = 5.1 years), we assessed the bi-factorial model of the TOS through factorial, convergent, and discriminant validity, reliability, and factorial invariance. We calculated the mean scores of the TOS factors and the frequency of behaviors of both orthorexia nervosa and healthy orthorexia. The new Portuguese version was well understood by participants, and the TOS bi-factorial model presented adequate psychometric properties and showed invariance in independent subsamples and in men and women. The mean scores were different between sexes only for orthorexia nervosa, with women obtaining higher values. The frequency of orthorexia nervosa behaviors was 5.3% and of healthy orthorexia was 41.2%. Based on these findings, the Brazilian Portuguese version of the TOS can be a useful tool for investigating orthorexia-like behaviors in future research.info:eu-repo/semantics/publishedVersio

Academic experience satisfaction of pharmacy and dentistry students

Objective: To assess the psychometric properties of the Academic Experience Satisfaction Scale (ESEA) applied to Pharmacy and Dentistry students and investigate the effect of demographic and course-related characteristics on the ESEA factors. Methods: This was a cross-sectional study. Academic satisfaction was assessed by the ESEA. Construct validity was measured by confirmatory factor analysis (CFI, TLI, RMSEA). The composite reliability (CR) and the ordinal α were estimated. Structural model was elaborated considering demographic and course-related characteristics as the independent variable and ESEA factors as the dependent variables. The model fit and path estimates significance (β) were evaluated (z test; α = 5%). Results: 544 students participated in the study (70.0% female; mean age: 21.4 ± 2.4 years; 52.4% dental students). ESEA showed adequate fit to the data (CFI = 0.90; TLI = 0.90; RMSEA = 0.064, CR and α ≥ 0.88). In general, students reported being satisfied with their academic experience. Dental students and those from the first and second years of the course showed greater satisfaction with the academic experience. Sex did not have a significant impact on any of the scale's factors. Conclusion: ESEA produced valid and reliable data. Course-related characteristics, but not demographic characteristics, significantly affected the academic experience of students.info:eu-repo/semantics/publishedVersio

Effect Of Immobilized Cells In Calcium Alginate Beads In Alcoholic Fermentation.

Saccharomyces cerevisiae cells were immobilized in calcium alginate and chitosan-covered calcium alginate beads and studied in the fermentation of glucose and sucrose for ethanol production. The batch fermentations were carried out in an orbital shaker and assessed by monitoring the concentration of substrate and product with HPLC. Cell immobilization in calcium alginate beads and chitosan-covered calcium alginate beads allowed reuse of the beads in eight sequential fermentation cycles of 10 h each. The final concentration of ethanol using free cells was 40 g L-1 and the yields using glucose and sucrose as carbon sources were 78% and 74.3%, respectively. For immobilized cells in calcium alginate beads, the final ethanol concentration from glucose was 32.9 ± 1.7 g L-1 with a 64.5 ± 3.4% yield, while the final ethanol concentration from sucrose was 33.5 ± 4.6 g L-1 with a 64.5 ± 8.6% yield. For immobilized cells in chitosan-covered calcium alginate beads, the ethanol concentration from glucose was 30.7 ± 1.4 g L-1 with a 61.1 ± 2.8% yield, while the final ethanol concentration from sucrose was 31.8 ± 6.9 g L-1 with a 62.1 ± 12.8% yield. The immobilized cells allowed eight 10 h sequential reuse cycles to be carried out with stable final ethanol concentrations. In addition, there was no need to use antibiotics and no contamination was observed. After the eighth cycle, there was a significant rupture of the beads making them inappropriate for reuse.33

Impact of body and orofacial appearance on life satisfaction among Brazilian adults

Aims 1. to elaborate a general model of physical appearance taking into account body image (BI) and orofacial appearance (OA) components; and 2. to evaluate the impact of BI and OA on life satisfaction among Brazilian adults. Methods This is a cross-sectional observational study. The cognitive, behavioral, affective, and satisfaction/ dissatisfaction aspects of BI, the satisfaction and psychosocial impact of OA, and life satisfaction were evaluated by self-reported psychometric scales. Principal Component Analysis and Parallel Analysis were performed. Structural equation models were elaborated to estimate the impact of BI and OA on life satisfaction. The fit of the models was verified and the significance of the path estimates (β) was evaluated using z-tests (α = 5%). Results A total of 1,940 individuals participated in the study (age: mean = 24.8, standard deviation = 5.7 years; females = 70.1%). In the male sample, three physical appearance factors were retained (OA, cognitive and behavioral components of BI, and affective and satisfaction/dissatisfaction components of BI). In the female sample, two factors were retained (OA and all components of BI). All factors had significant impact on life satisfaction (β = |0.26|-|0.48|, p<0.001) in both samples. Individuals dissatisfied with BI and OA had lower levels of life satisfaction. For men, the affective and satisfaction components of BI had a greater impact on life satisfaction (β = 0.48, p<0.001) than the other factors (β =] -0.30;-0.25[, p<0.001). For women, both BI and OA had a similar impact (BI: β = -0.30, p<0.001; OA: β = -0.32, p<0.001). Conclusion BI and OA formed distinct clusters in the physical appearance evaluation. Physical appearance was perceived differently by men and women, fostering discussion about the sociocultural construction of the body. BI and OA had a significant impact on life satisfaction and should be considered in assessment and treatment protocols.info:eu-repo/semantics/publishedVersio

European Organization for Research and Treatment of Cancer Quality of Life Questionnaire Core 30: factorial models to Brazilian cancer patients

Objective To evaluate the psychometric properties of the seven theoretical models proposed in the literature for European Organization for Research and Treatment of Cancer Quality of Life Questionnaire Core 30 (EORTC QLQ-C30), when applied to a sample of Brazilian cancer patients. Methods Content and construct validity (factorial, convergent, discriminant) were estimated. Confirmatory factor analysis was performed. Convergent validity was analyzed using the average variance extracted. Discriminant validity was analyzed using correlational analysis. Internal consistency and composite reliability were used to assess the reliability of instrument. Results A total of 1,020 cancer patients participated. The mean age was 53.3±13.0 years, and 62% were female. All models showed adequate factorial validity for the study sample. Convergent and discriminant validities and the reliability were compromised in all of the models for all of the single items referring to symptoms, as well as for the "physical function" and "cognitive function" factors. Conclusion All theoretical models assessed in this study presented adequate factorial validity when applied to Brazilian cancer patients. The choice of the best model for use in research and/or clinical protocols should be centered on the purpose and underlying theory of each model.FAPESPinfo:eu-repo/semantics/publishedVersio

Relationship between attention to body shape, social physique anxiety, and personal characteristics of Brazilians: A structural equation model

Abstract: People can develop eating disorders due to excessive body image concerns. The primary objective of this study was to examine the relationship between attention to body shape, social physique anxiety, and personal characteristics in a sample of Brazilians. The secondary objective was to evaluate the correlation of the constructs with the participants’ body composition. First, 1795 individuals (70% female; Mage = 25.5 ± 6.6 years) completed the Attention to Body Shape Scale, the Social Physique Anxiety Scale, and a sociodemographic questionnaire. Then, 286 participants (58% female; Mage = 25.3 ± 5.7 years) underwent a bioimpedance exam to identify body composition. Structural equation modeling was used to estimate the relationship between the variables. The greater the attention to body shape, the greater the expectations of negative physical evaluation and the less comfort with physical presentation. Younger age, female gender, consumption of supplements/substances for body change, restrictive diets, physical inactivity, poor self-assessment of food quality, and overweight/obesity were related to negative body concerns. An expectation of negative physical evaluation was positively correlated with body fat and negatively with muscle mass. Comfort with physical presentation was negatively correlated with fat and positively with muscle. These results can support preventive strategies aimed at reducing eating disorders resulting from body image concerns.info:eu-repo/semantics/publishedVersio