102 research outputs found
Effects of Aluminum Oxide Nanoparticles on the Growth, Development, and microRNA Expression of Tobacco (Nicotiana tabacum)
Nanoparticles are a class of newly emerging environmental pollutions. To date, few experiments have been conducted to investigate the effect nanoparticles may have on plant growth and development. It is important to study the effects nanoparticles have on plants because they are stationary organisms that cannot move away from environmental stresses like animals can, therefore they must overcome these stresses by molecular routes such as altering gene expression. microRNAs (miRNA) are a newly discovered, endogenous class of post-transcriptional gene regulators that function to alter gene expression by either targeting mRNAs for degradation or inhibiting mRNAs translating into proteins. miRNAs have been shown to mediate abiotic stress responses such as drought and salinity in plants by altering gene expression, however no study has been performed on the effect of nanoparticles on the miRNA expression profile; therefore our aim in this study was to classify if certain miRNAs play a role in plant response to Al2O3 nanoparticle stress. In this study, we exposed tobacco (Nicotiana tabacum) plants (an important cash crop as well as a model organism) to 0%, 0.1%, 0.5%, and 1% Al2O3 nanoparticles and found that as exposure to the nanoparticles increased, the average root length, the average biomass, and the leaf count of the seedlings significantly decreased. We also found that miR395, miR397, miR398, and miR399 showed an extreme increase in expression during exposure to 1% Al2O3 nanoparticles as compared to the other treatments and the control, therefore these miRNAs may play a key role in mediating plant stress responses to nanoparticle stress in the environment. The results of this study show that Al2O3 nanoparticles have a negative effect on the growth and development of tobacco seedlings and that miRNAs may play a role in the ability of plants to withstand stress to Al2O3 nanoparticles in the environment
Ring distributions leading to species formation: a global topographic analysis of geographic barriers associated with ring species
<p>Abstract</p> <p>Background</p> <p>In the mid 20<sup>th </sup>century, Ernst Mayr and Theodosius Dobzhansky championed the significance of circular overlaps or ring species as the perfect demonstration of speciation, yet in the over 50 years since, only a handful of such taxa are known. We developed a topographic model to evaluate whether the geographic barriers that favor processes leading to ring species are common or rare, and to predict where other candidate ring barriers might be found.</p> <p>Results</p> <p>Of the 952,147 geographic barriers identified on the planet, only about 1% are topographically similar to barriers associated with known ring taxa, with most of the likely candidates occurring in under-studied parts of the world (for example, marine environments, tropical latitudes). Predicted barriers separate into two distinct categories: (i) single cohesive barriers (< 50,000 km<sup>2</sup>), associated with taxa that differentiate at smaller spatial scales (salamander: <it>Ensatina eschscholtzii</it>; tree: <it>Acacia karroo</it>); and (ii) composite barriers - formed by groups of barriers (each 184,000 to 1.7 million km<sup>2</sup>) in close geographic proximity (totaling 1.9 to 2.3 million km<sup>2</sup>) - associated with taxa that differentiate at larger spatial scales (birds: <it>Phylloscopus trochiloide</it>s and <it>Larus </it>(sp. <it>argentatus </it>and <it>fuscus</it>)). When evaluated globally, we find a large number of cohesive barriers that are topographically similar to those associated with known ring taxa. Yet, compared to cohesive barriers, an order of magnitude fewer composite barriers are similar to those that favor ring divergence in species with higher dispersal.</p> <p>Conclusions</p> <p>While these findings confirm that the topographic conditions that favor evolutionary processes leading to ring speciation are, in fact, rare, they also suggest that many understudied natural systems could provide valuable demonstrations of continuous divergence towards the formation of new species. Distinct advantages of the model are that it (i) requires no <it>a priori </it>information on the relative importance of features that define barriers, (ii) can be replicated using any kind of continuously distributed environmental variable, and (iii) generates spatially explicit hypotheses of geographic species formation. The methods developed here - combined with study of the geographical ecology and genetics of taxa in their environments - should enable recognition of ring species phenomena throughout the world.</p
Applying psychological learning theory to helping students overcome learned difficulties in mathematics: An alternative approach to intervention
The appearance of systematic errors in computation suggests relatively unlinked computational knowledge to conceptual knowledge, and hence difficulties with forward learning of mathematics. The provision of programs of good teaching, where concrete materials are used to exemplify and thus legitimize algorithmic processes, frequently are not effective for use with upper primary students: systematic errors often resurface. A novel and quite alternate approach to intervention is the Old Way/New Way (O/N) strategy (Lyndon, 1989) based on psychological principles of memory, forgetting and interference. In this article, issues associated with intervention, systematic errors and upper primary students are addressed through a discussion of results of previous research into seventh graders' subtraction knowledge development by overcoming error patterns in subtraction computation. By comparing re-teaching strategies and O/N, it is proposed that both good teaching and effective intervention strategies should be integral to the craft of teaching, particularly in the middle school
From evolutionary computation to the evolution of things
Evolution has provided a source of inspiration for algorithm designers since the birth of computers. The resulting field, evolutionary computation, has been successful in solving engineering tasks ranging in outlook from the molecular to the astronomical. Today, the field is entering a new phase as evolutionary algorithms that take place in hardware are developed, opening up new avenues towards autonomous machines that can adapt to their environment. We discuss how evolutionary computation compares with natural evolution and what its benefits are relative to other computing approaches, and we introduce the emerging area of artificial evolution in physical systems
The role of sex separation in neutral speciation
Neutral speciation mechanisms based on isolation by distance and sexual
selection, termed topopatric, have recently been shown to describe the observed
patterns of abundance distributions and species-area relationships. Previous
works have considered this type of process only in the context of hermaphrodic
populations. In this work we extend a hermaphroditic model of topopatric
speciation to populations where individuals are explicitly separated into males
and females. We show that for a particular carrying capacity speciation occurs
under similar conditions, but the number of species generated decreases as
compared to the hermaphroditic case. Evolution results in fewer species having
more abundant populations.Comment: 18 pages + 8 figure
Effect of VX-770 in Persons with Cystic Fibrosis and the G551D- CFTR Mutation
A new approach in the treatment of cystic fibrosis involves improving the function of mutant cystic fibrosis transmembrane conductance regulator (CFTR). VX-770, a CFTR potentiator, has been shown to increase the activity of wild-type and defective cell-surface CFTR in vitro
New synonymies and generic changes in the Lygaeidae (Hemiptera-Heteroptera)
Volume: 73Start Page: 235End Page: 23
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