Timing of progression from Chlamydia trachomatis infection to pelvic inflammatory disease: a mathematical modelling study

PMCID: PMC3505463The electronic version of this article is the complete one and can be found online at: http://www.biomedcentral.com/1471-2334/12/187. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited

Effect of Cavtratin, a Caveolin-1 Scaffolding Domain Peptide, on Oligodendroglial Signaling Cascades

Caveolin and caveolin containing rafts are involved in the signaling of growth factors in various cell types. Previous reports of our lab indicated a co-localization of caveolin and the high affinity nerve growth factor (NGF) receptor tyrosine kinase A (TrkA). Mutual effects have been observed among which a caveolin-1 knock-down resulted in an impairment of the NGF signaling cascade rather than in an increase of activity as expected from other growth factor reports. On the other hand, an over-expression of caveolin-1 impaired the NGF stimulated activity of p42/44 mitogen activated protein kinases (MAPK). In this study, we used a caveolin-1 scaffolding domain (CSD) peptide (cavtratin) of which an inhibitory effect on growth factor receptors was reported. Our data showed that cavtratin suppresses the NGF-induced phosphorylation of TrkA as well as the activation of MAPK in porcine oligodendrocytes significantly

Two new pulsating low-mass pre-white dwarfs or SX Phenix stars?*

Context. The discovery of pulsations in low-mass stars opens an opportunity for probing their interiors and to determine their evolution, by employing the tools of asteroseismology. Aims. We aim to analyze high-speed photometry of SDSSJ145847.02$+$070754.46 and SDSSJ173001.94$+$070600.25 and discover brightness variabilities. In order to locate these stars in the $T_{\rm eff} - \log g$ diagram we fit optical spectra (SDSS) with synthetic non-magnetic spectra derived from model atmospheres. Methods. To carry out this study, we used the photometric data obtained by us for these stars with the 2.15m telescope at CASLEO, Argentina. We analyzed their light curves and we apply the Discrete Fourier Transform to determine the pulsation frequencies. Finally, we compare both stars in the $T_{\rm eff} - \log g$ diagram, with known two pre-white dwarfs, seven pulsating pre-ELM white dwarf stars, $\delta$ Scuti and SX Phe stars. Results. We report the discovery of pulsations in SDSSJ145847.02$+$070754.46 and SDSSJ173001.94$+$070600.25. We determine their effective temperature and surface gravity to be $T_{\rm eff}$ = 7 972 $\pm$ 200 K, $\log g$ = 4.25 $\pm$ 0.5 and $T_{\rm eff}$ = 7 925 $\pm$ 200 K, $\log g$ = 4.25 $\pm$ 0.5, respectively. With these parameters these new pulsating low-mass stars can be identified with either ELM white dwarfs (with ~ 0.17 Mo) or more massive SX Phe stars. We identified pulsation periods of 3 278.7 and 1 633.9 s for SDSSJ145847.02$+$070754.46 and a pulsation period of 3 367.1 s for SDSSJ173001.94$+$070600.25. These two new objects together with those of Maxted et al. (2013, 2014) indicate the possible existence of a new instability domain towards the late stages of evolution of low-mass white dwarf stars, although their identification with SX Phe stars cannot be discarded.Comment: 5 pages, 5 figures, 1 table, accepted for publication in A&A

Poly(ADP-ribose)glycohydrolase is an upstream regulator of Ca2+ fluxes in oxidative cell death

Oxidative DNA damage to cells activates poly(ADP-ribose)polymerase-1 (PARP-1) and the poly(ADP-ribose) formed is rapidly degraded to ADP-ribose by poly(ADP-ribose)glycohydrolase (PARG). Here we show that PARP-1 and PARG control extracellular Ca2+ fluxes through melastatin-like transient receptor potential 2 channels (TRPM2) in a cell death signaling pathway. TRPM2 activation accounts for essentially the entire Ca2+ influx into the cytosol, activating caspases and causing the translocation of apoptosis inducing factor (AIF) from the inner mitochondrial membrane to the nucleus followed by cell death. Abrogation of PARP-1 or PARG function disrupts these signals and reduces cell death. ADP-ribose-loading of cells induces Ca2+ fluxes in the absence of oxidative damage, suggesting that ADP-ribose is the key metabolite of the PARP-1/PARG system regulating TRPM2. We conclude that PARP-1/PARG control a cell death signal pathway that operates between five different cell compartments and communicates via three types of chemical messengers: a nucleotide, a cation, and protein

An upper limit to the secular variation of the gravitational constant from white dwarf stars

A variation of the gravitational constant over cosmological ages modifies the main sequence lifetimes and white dwarf cooling ages. Using an state-of-the-art stellar evolutionary code we compute the effects of a secularly varying G on the main sequence ages and, employing white dwarf cooling ages computed taking into account the effects of a running G, we place constraints on the rate of variation of Newton's constant. This is done using the white dwarf luminosity function and the distance of the well studied open Galactic cluster NGC 6791. We derive an upper bound G'/G ~ -1.8 10^{-12} 1/yr. This upper limit for the secular variation of the gravitational constant compares favorably with those obtained using other stellar evolutionary properties, and can be easily improved if deep images of the cluster allow to obtain an improved white dwarf luminosity function.Comment: 15 pages, 4 figures, accepted for publication in JCA

Relation of Gallbladder Motility to Viscosity and Composition of Gallbladder Bile in Patients with Cholesterol Gallstones

Background/Aims: Increased viscosity and supersaturation of cholesterol in gallbladder bile, as well as an impaired motility of the gallbladder, are considered to be important factors in the pathogenesis of cholesterol gallstones. However, the relation of these parameters has not yet been determined. Material and Methods: Bile viscosity (mPa.s) was measured by rotation viscosimetry and the composition of gallbladder bile was determined using standard methodology. Gallbladder motility was calculated as ejection fraction in percent of total volume 45 min after a test meal using ultrasonography in patients with gallstones prior to elective cholecystectomy. Results: The study included 35 patients with cholesterol gallstones. Viscosity of gallbladder bile ranged between 0.9 and 12.5 mPa.s (median 2.2 mPa.s) and an ejection fraction of the gallbladder of 55.4 +/- 18.3% (mean +/- SD) was determined. No significant correlation (r = 0.19, p < 0.2) between the 2 parameters could be calculated. Analysis of the composition of gallbladder bile revealed a positive correlation of all components to biliary viscosity but not to the motility of the gallbladder, with the exceptions of a negative correlation (r = 0.39, p < 0.02) between mucin concentration and the ejection fraction at 45 min after the test meal. Conclusions: The motility of the gallbladder appears to be unrelated to the viscosity of gallbladder bile or gallbladder bile composition. The negative correlation between the ejection fraction of the gallbladder and mucin concentration of gallbladder bile suggests that chronic inflammation of the gallbladder wall is associated with both an impaired motility of the gallbladder and increased mucin release into gallbladder bile. Copyright (C) 2009 S. Karger AG, Base

Follow-up Studies of the Pulsating Magnetic White Dwarf SDSS J142625.71+575218.3

We present a follow-up analysis of the unique magnetic luminosity-variable carbon-atmosphere white dwarf SDSS J142625.71+575218.3. This includes the results of some 106.4 h of integrated light photometry which have revealed, among other things, the presence of a new periodicity at 319.720 s which is not harmonically related to the dominant oscillation (417.707 s) previously known in that star. Using our photometry and available spectroscopy, we consider the suggestion made by Montgomery et al. (2008) that the luminosity variations in SDSS J142625.71+575218.3 may not be caused by pulsational instabilities, but rather by photometric activity in a carbon-transferring analog of AM CVn. This includes a detailed search for possible radial velocity variations due to rapid orbital motion on the basis of MMT spectroscopy. At the end of the exercise, we unequivocally rule out the interacting binary hypothesis and conclude instead that, indeed, the luminosity variations are caused by g-mode pulsations as in other pulsating white dwarfs. This is in line with the preferred possibility put forward by Montgomery et al. (2008).Comment: 11 pages in emulateApJ, 12 figures, accepted for publication in Ap

Revisiting the theoretical DBV (V777 Her) instability strip: the MLT theory of convection

We reexamine the theoretical instability domain of pulsating DB white dwarfs (DBV or V777 Her variables). We performed an extensive $g$-mode nonadiabatic pulsation analysis of DB evolutionary models considering a wide range of stellar masses, for which the complete evolutionary stages of their progenitors from the ZAMS, through the thermally pulsing AGB and born-again phases, the domain of the PG1159 stars, the hot phase of DO white dwarfs, and then the DB white dwarf stage have been considered. We explicitly account for the evolution of the chemical abundance distribution due to time-dependent chemical diffusion processes. We examine the impact of the different prescriptions of the MLT theory of convection and the effects of small amounts of H in the almost He-pure atmospheres of DB stars on the precise location of the theoretical blue edge of the DBV instability strip.Comment: Proceedings, 16th European White Dwarf Workshop, Barcelona, 200