Determinación cuantitativa de indigotina e indirubina en el índigo natural mediante cromatografía líquida de alto rendimiento (HPLC)

A high-performance liquid chromatographic separation and quantitative method using a RP-18 columneluted isocratically with methanol and water (73:27, v/v) was developed to analyze the isomeric activecompounds, indigotin and indirubin, present in the chloroform extract of indigo naturalis. Standardizedsolutions of indigotin and indirubin were used to evaluate linearity, precision (repeatability), accuracyand specificity of the method were evaluated. The results exhibited a linearity (r2 > 0.999) of between1.8 - 16.2 μg/ml and 1.6 - 14.4 μg/ml for indigotin and indirubin, respectively. A high degree ofspecificity and robustness, as well as repeatability (relative standard deviation less than 5% for bothstandards and the extracts) and accuracy (% recoveries greater than 95%) were also achievedSe desarrolló un método de cromatografía líquida de alto rendimiento cuantitativa y de separaciónutilizando una columna RP-18 eluida isocráticamente con metanol y agua (73:27, v/v) para analizar loscompuestos activos isoméricos indigotina e indirubina presentes en el extracto de cloroformo del índigonatural. Se utilizaron soluciones estandarizadas de indigotina e indirubina para evaluar la linealidad, laprecisión (replicabilidad), la exactitud y la especificidad del método. Los resultados mostraron unalinealidad (r2 > 0,999) de entre 1,8 y 16,2 μg/ml para la indigotina y entre 1,6 y 14,4 μg/ml para laindirubina. También se obtuvo un alto grado de especificidad, robustez, replicabilidad (desviaciónestándar relativa inferior al 5% para estándares y extractos) y exactitud (% de recuperaciones superioral 95%)

Pharmacognostic and chemical studies on the leaves of Rhinacanthus nasutus

No Abstract. Nigerian Journals of Natural Products and Medicine Vol. 10 () 2006: pp.1-

Quantitative HPLC determination of indigotin and indirubin in indigo naturalis

Se desarrolló un método de cromatografía líquida de alto rendimiento cuantitativa y de separación utilizando una columna RP-18 eluida isocráticamente con metanol y agua (73:27, v/v) para analizar los compuestos activos isoméricos indigotina e indirubina presentes en el extracto de cloroformo del índigo natural. Se utilizaron soluciones estandarizadas de indigotina e indirubina para evaluar la linealidad, la precisión (replicabilidad), la exactitud y la especificidad del método. Los resultados mostraron una linealidad (r2 > 0,999) de entre 1,8 y 16,2 μg/ml para la indigotina y entre 1,6 y 14,4 μg/ml para la indirubina. También se obtuvo un alto grado de especificidad, robustez, replicabilidad (desviación estándar relativa inferior al 5% para estándares y extractos) y exactitud (% de recuperaciones superior al 95%).A high-performance liquid chromatographic separation and quantitative method using a RP-18 column eluted isocratically with methanol and water (73:27, v/v) was developed to analyze the isomeric active compounds, indigotin and indirubin, present in the chloroform extract of indigo naturalis. Standardized solutions of indigotin and indirubin were used to evaluate linearity, precision (repeatability), accuracy and specificity of the method were evaluated. The results exhibited a linearity (r2 > 0.999) of between 1.8 - 16.2 μg/ml and 1.6 - 14.4 μg/ml for indigotin and indirubin, respectively. A high degree of specificity and robustness, as well as repeatability (relative standard deviation less than 5% for both standards and the extracts) and accuracy (% recoveries greater than 95%) were also achieved

Determinación cuantitativa de indigotina e indirubina en el índigo natural mediante cromatografía líquida de alto rendimiento (HPLC)

Se desarrolló un método de cromatografía líquida de alto rendimiento cuantitativa y de separación utilizando una columna RP-18 eluida isocráticamente con metanol y agua (73:27, v/v) para analizar los compuestos activos isoméricos indigotina e indirubina presentes en el extracto de cloroformo del índigo natural. Se utilizaron soluciones estandarizadas de indigotina e indirubina para evaluar la linealidad, la precisión (replicabilidad), la exactitud y la especificidad del método. Los resultados mostraron una linealidad (r r2 > 0,999) de entre 1,8 y 16,2 µg/ml para la indigotina y entre 1,6 y 14,4 µg/ml para la indirubina. También se obtuvo un alto grado de especificidad, robustez, replicabilidad (desviación estándar relativa inferior al 5% para estándares y extractos) y exactitud (% de recuperaciones superior al 95%)

Rhinacanthin C ameliorates hyperglycaemia, hyperlipidemia and pancreatic destruction in streptozotocin–nicotinamide induced adult male diabetic rats

Effect of Rhinacanthin C on hyperglycaemia, hyperlipidemia and pancreatic dysfunction in diabetes was investigated. In-vitro effect of Rhinacanthin C on glucose uptake was studied in 3T3-L1 cell line. Meanwhile, in-vivo effect of 28-days treatment with 5 mg/kg/day or 20 mg/kg/day Rhinacanthin C was studied in streptozotocin–nicotinamide induced male diabetic rats. Following completion of treatment, fasting blood glucose (FBG), HbA1c, insulin and lipid profile levels were measured by biochemical assays. Histopathological changes in pancreas were observed by light microscopy while levels of pancreatic oxidative stress were determined by enzymatic assays. Expression of insulin, TNFα, Ikkβ and caspase-3 in pancreas were quantified by immunohistochemistry. Molecular docking was used to identify interactions between Rhinacathin C with SOD or GPx enzymes. Dose-dependent increase in glucose uptake was observed with increasing doses of Rhinacathin C. Plasma FBG, HbA1c and lipid profile except LDL levels and pancreatic malonaldehyde level were reduced but serum insulin and pancreatic anti-oxidative enzymes (SOD, CAT and GPx) levels were increased in diabetic rats receiving Rhinacanthin C treatment. Decreased pancreatic histopathological changes with higher pancreatic insulin and Glut-2 levels but lower TNFα, Ikkβ and caspase-3 levels were observed in diabetic rats receiving Rhinacanthin C (P<0.05 compared to non-treated diabetic rats). In diabetic rats which received Rhinacathin C, changes in the above parameters did not achieve the value in non-diabetic rats. Docking shows Rhinacathin C possesses high degree interactions with SOD and GPx. By possessing these effects, Rhinacanthin C could be used as agent to alleviate pancreatic and other complications in diabetes