Polysaccharide utilization loci and nutritional specialization in a dominant group of butyrate-producing human colonic Firmicutes

Acknowledgements The Rowett Institute of Nutrition and Health (University of Aberdeen) receives financial support from the Scottish Government Rural and Environmental Sciences and Analytical Services (RESAS). POS is a PhD student supported by the Scottish Government (RESAS) and the Science Foundation Ireland, through a centre award to the APC Microbiome Institute, Cork, Ireland. Data Summary The high-quality draft genomes generated in this work were deposited at the European Nucleotide Archive under the following accession numbers: 1. Eubacterium rectale T1-815; CVRQ01000001–CVRQ0100 0090: http://www.ebi.ac.uk/ena/data/view/PRJEB9320 2. Roseburia faecis M72/1; CVRR01000001–CVRR010001 01: http://www.ebi.ac.uk/ena/data/view/PRJEB9321 3. Roseburia inulinivorans L1-83; CVRS01000001–CVRS0 100 0151: http://www.ebi.ac.uk/ena/data/view/PRJEB9322Peer reviewedPublisher PD

Aspects of slurry management on pig farms.

End of Project ReportThe objectives of manure or slurry management on intensive pig farms are the provision of adequate slurry storage capacity and the efficient recycling of the slurry nutrients for crop production. However, recent surveys of pig slurry dry matter suggest there is excessive dilution of raw pig slurry with water. This has two important implications for management. The first is greater storage capacity will be required due to the increased volume of slurry generated. Slurry storage is expensive. For example, a 350 sow unit adding 10 weeks storage needs to invest £50,000. Secondly, evidence from the literature indicates an improved slurry nitrogen efficiency with the more dilute manure. The results of field trials showed that higher dry matter pig slurries reduced the relative efficiency of pig slurry nitrogen for second cut silage production. This is probably linked to reduced ammonia volatilisation losses, consequent to the less viscous nature of dilute slurry which permits a more rapid infiltration of the ammonium nitrogen into the soil. The use of a band spreader or shallow injection rather than the conventional splash plate were shown to increase the efficiency of pig slurry nitrogen for grass silage production. Therefore, the potential for the higher pig slurry dry matter, required for cost effective storage/ handling costs, to reduce the efficiency of its nitrogen for grass silage production can be partially offset by using band spreaders or shallow injection spreading systems. These have the added advantage of reducing odour emissions from the land spreading operation.Teagasc Walsh Fellowship Programm

Cool Jupiters greatly outnumber their toasty siblings : Occurrence rates from the Anglo-Australian Planet Search

This article has been accepted for publication in Monthly Notices of the Royal Astronomical Society ©2019 The Authors. Published by Oxford University Press on behalf of the Royal Astronomical Society. All rights reserved.Our understanding of planetary systems different to our own has grown dramatically in the past 30 yr. However, our efforts to ascertain the degree to which the Solar system is abnormal or unique have been hindered by the observational biases inherent to the methods that have yielded the greatest exoplanet hauls. On the basis of such surveys, one might consider our planetary system highly unusual - but the reality is that we are only now beginning to uncover the true picture. In this work, we use the full 18-yr archive of data from the Anglo-Australian Planet Search to examine the abundance of 'cool Jupiters' - analogues to the Solar system's giant planets, Jupiter and Saturn. We find that such planets are intrinsically far more common through the cosmos than their siblings, the hot Jupiters.We find that the occurrence rate of such 'cool Jupiters' is 6.73 +2.09 -1.13 per cent, almost an order of magnitude higher than the occurrence of hot Jupiters (at 0.84 +0.70 -0.20 per cent). We also find that the occurrence rate of giant planets is essentially constant beyond orbital distances of ~1 au. Our results reinforce the importance of legacy radial velocity surveys for the understanding of the Solar system's place in the cosmos.Peer reviewe

Exoplanet properties from Lick, Keck and AAT

Doppler-shift measurements with a remarkable precision of Δλ/λ=3×10-9, corresponding to velocities of 1 m s-1, have been made repeatedly of 2500 stars located within 300 light years. The observed gravitational perturbations of the stars have revealed 250 orbiting planets, with 27 that cross in front of the host star, blocking a fraction of the starlight to allow measurement of the planet's mass, radius and density. Two new discoveries are the first good analog of Jupiter (HD 154345b) and the first system of five planets (55 Cancri). The predominantly eccentric orbits of exoplanets probably result from planet planet gravitational interactions or angular momentum exchange by mean-motion resonances. The planet mass distribution ranges from ~15 MJUP to as low as ~5 MEarth and rises toward lower masses as dN/dM~M-1.1. The distribution with orbital distance, a, rises (in logarithmic intervals) as dN/d log a~a+0.4. Extrapolation and integration suggests that 19% of all Sun-like stars harbor a gas-giant planet within 20 AU, but there remains considerable incompleteness for large orbits. Beyond 20 AU, the occurrence of gas-giant planets may be less than a few per cent as protoplanetary disk material there has lower densities and is vulnerable to destruction. Jupiter-mass planets occur more commonly around more massive stars than low mass stars. The transit of the Neptune-mass planet, Gliese 436b, yields a density of 1.55 g cm-3 suggesting that its interior has an iron silicate core surrounded by an envelope of water ice and an outer H He shell. Planets with masses as low as five Earth-masses may be commonly composed of iron nickel, rock and water along with significant amounts of H and He, making the term 'super-Earth' misleading. The transiting planet HD147506b has high orbital eccentricity but no significant orbital inclination to the line of sight, presenting a puzzle about its history. Its orbit together with the mean motion resonances of 4 of the 22 multi-planet systems provides further evidence for the role of planet planet interactions in shaping planetary architectures

Aberrant phenotype in human endothelial cells of diabetic origin: Implications for saphenous vein graft failure?

Type 2 diabetes (T2DM) confers increased risk of endothelial dysfunction, coronary heart disease, and vulnerability to vein graft failure after bypass grafting, despite glycaemic control. This study explored the concept that endothelial cells (EC) cultured from T2DM and nondiabetic (ND) patients are phenotypically and functionally distinct. Cultured human saphenous vein-(SV-) EC were compared between T2DM and ND patients in parallel. Proliferation, migration, and in vitro angiogenesis assays were performed; western blotting was used to quantify phosphorylation of Akt, ERK, and eNOS. The ability of diabetic stimuli (hyperglycaemia, TNF-α, and palmitate) to modulate angiogenic potential of ND-EC was also explored. T2DM-EC displayed reduced migration (30%) and angiogenesis (40%) compared with ND-EC and a modest, nonsignificant trend to reduced proliferation. Significant inhibition of Akt and eNOS, but not ERK phosphorylation, was observed in T2DM cells. Hyperglycaemia did not modify ND-EC function, but TNF-α and palmitate significantly reduced angiogenic capacity (by 27% and 43%, resp.), effects mimicked by Akt inhibition. Aberrancies of EC function may help to explain the increased risk of SV graft failure in T2DM patients. This study highlights the importance of other potentially contributing factors in addition to hyperglycaemia that may inflict injury and long-term dysfunction to the homeostatic capacity of the endothelium

Detection of Planetary and Stellar Companions to Neighboring Stars via a Combination of Radial Velocity and Direct Imaging Techniques

13 pages, 6 figures, 4 tables, accepted for publication in the Astronomical Journal (submitted 25 Feb 2019; accepted 28 April 2019). Machine readable tables and Posteriors from the RadVel fits are available here: http://stephenkane.net/rvfits.tarThe sensitivities of radial velocity (RV) surveys for exoplanet detection are extending to increasingly longer orbital periods, where companions with periods of several years are now being regularly discovered. Companions with orbital periods that exceed the duration of the survey manifest in the data as an incomplete orbit or linear trend, a feature that can either present as the sole detectable companion to the host star, or as an additional signal overlain on the signatures of previously discovered companion(s). A diagnostic that can confirm or constrain scenarios in which the trend is caused by an unseen stellar rather than planetary companion is the use of high-contrast imaging observations. Here, we present RV data from the Anglo-Australian Planet Search (AAPS) for 20 stars that show evidence of orbiting companions. Of these, six companions have resolved orbits, with three that lie in the planetary regime. Two of these (HD 92987b and HD 221420b) are new discoveries. Follow-up observations using the Differential Speckle Survey Instrument (DSSI) on the Gemini South telescope revealed that 5 of the 20 monitored companions are likely stellar in nature. We use the sensitivity of the AAPS and DSSI data to place constraints on the mass of the companions for the remaining systems. Our analysis shows that a planetary-mass companion provides the most likely self-consistent explanation of the data for many of the remaining systems.Peer reviewedFinal Accepted Versio