558 research outputs found
Unnatural Fornication Cases under State-Socialism: A Hungarian–Slovenian Comparative Social-Historical Approach
Using Citizen Scientists to Evaluate Light Traps for Catching Brown Marmorated Stink Bugs in Homes in Virginia
More and more, citizen scientists are playing an integral role in research studies. This has been particularly evident as entomologists unravel the biology, spread, and management of the brown marmorated stink bug, which has plagued many homeowners in the mid-Atlantic U.S. in recent years. We used citizen scientists to evaluate different indoor light traps for catching the bugs in houses. Throughout the late winter and early spring months, these traps were tested inside homes and enabled us to determine that the most efficacious trap was an aluminum foil water pan trap, developed by—you guessed it—a citizen scientist
Pesticide Use Changes in New York Vegetables: 1978 to 1998
Pesticide use patterns in 1978 and 1998 were compared for 15 vegetable crops grown in New York State. Insecticide use decreased in almost all vegetables over this period, with an overall decline of 65%. Total herbicide use declined 24%, while fungicide use increased 76%. Within crops, potatoes and onions received more than 60% of all pesticide use on vegetables. Large declines in pesticide use occurred in some crops and usually were associated with the substitution of low use-rate for high use-rate insecticides or herbicides. Strategies for future reductions in pesticide use are discussed
An On-Line Survey Process for Assessing Impact of an Email-Delivered Pest Advisory
IPM specialists simplified the entry, collection, editing, and distribution of pest alerts by creating a new Web site, the Virginia Ag Pest Advisory. A simple on-line survey was used to assess the usefulness of the advisory. The on-line survey was low-cost and required less effort than conventional surveys. Such a system provides feedback from users, which can be used to improve Extension programs and generates results to be used in reporting impact data
Sublethal Effects of the Insecticide Pyrifluquinazon on the European Honey Bee (Hymenoptera: Apidae)
Pyrifluquinazon (PQZ) is an Insecticide Resistance Action Committee (IRAC) Group 9 insecticide that has recently been registered for use in the United States for control of soft-bodied sucking insect pests. Although it has been classified as practically nontoxic to honey bees, Apis mellifera L. (Hymenoptera: Apidae), based on acute contact bioassays, additional information on sublethal effects of this insecticide on honey bees is lacking. Using a combination of laboratory assays with video movement tracking software and near-field evaluations of colonies foraging in a high-tunnel experiment, we determined that, when fed PQZ at a concentration of 84 mg active ingredient (ai)/liter (= ppm) in sugar water, a reduction in overall movement by the foraging worker bees was observed. However, when provided with honey reserves in the hive, honey bees rejected the PQZ-treated sugar water. These results indicate that, if ingested at levels of 84 mg ai/liter, PQZ could have a negative effect on honey bee behavior; however, honey bee workers appear to be able to detect the presence of PQZ in their food and reject it
First analysis of solar structures in 1.21 mm full-disc ALMA image of the Sun
Various solar features can be seen on maps of the Sun in the mm and sub-mm
wavelength range. The recently installed Atacama Large Millimeter/submillimeter
Array (ALMA) is capable of observing the Sun in that wavelength range with an
unprecedented spatial, temporal and spectral resolution. To interpret solar
observations with ALMA the first important step is to compare ALMA maps with
simultaneous images of the Sun recorded in other spectral ranges. First we
identify different structures in the solar atmosphere seen in the optical, IR
and EUV parts of the spectrum (quiet Sun (QS), active regions (AR), prominences
on the disc, magnetic inversion lines (IL), coronal holes (CH) and coronal
bright points (CBPs)) in a full disc solar ALMA image. The second aim is to
measure the intensities (brightness temperatures) of those structures and
compare them with the corresponding QS level. A full disc solar image at 1.21
mm obtained on December 18, 2015 during a CSV-EOC campaign with ALMA is
calibrated and compared with full disc solar images from the same day in
H\alpha, in He I 1083 nm core, and with SDO images (AIA at 170 nm, 30.4 nm,
21.1 nm, 19.3 nm, and 17.1 nm and HMI magnetogram). The brightness temperatures
of various structures are determined by averaging over corresponding regions of
interest in the ALMA image. Positions of the QS, ARs, prominences on the disc,
ILs, CHs and CBPs are identified in the ALMA image. At 1.21 mm ARs appear as
bright areas (but sunspots are dark), while prominences on the disc and CHs are
not discernible from the QS background, although having slightly less intensity
than surrounding QS regions. ILs appear as large, elongated dark structures and
CBPs correspond to ALMA bright points. These results are in general agreement
with sparse earlier measurements at similar wavelengths. The identification of
CBPs represents the most important new result.Comment: 9 pages, 3 figure
Differential immunity in pigs with high and low responses to porcine reproductive and respiratory syndrome virus infection
One hundred Hampshire × Duroc crossbred pigs (HD) and 100 NE Index line (I) pigs were infected with porcine reproductive and respiratory syndrome (PRRS) virus and evaluated for resistance/susceptibility. Controls (100/line) were uninfected littermates to the infected pigs. Viremia, change in weight (WTΔ), and rectal temperature at 0, 4, 7, and 14 d postinfection were recorded. Lung, bronchial lymph node (BLN), and blood tissue were collected at necropsy (14 d postinfection). The first principal component from principal component analyses of all variables was used to rank the pigs for phenotypic response to PRRS virus. Low responders (low PRRS burden) had high WTΔ, low viremia, and few lung lesions; high responders (high PRRS burden) had low WTΔ, high viremia, and many lesions. The RNA was extracted from lung and BLN tissue of the 7 highest and 7 lowest responders per line and from each of their littermates. Expression of 11 innate and T helper 1 immune markers was evaluated with cDNA in a 2 × 2 × 2 factorial design. Significant upregulation in lung, lymph, or both of infected pigs relative to controls occurred for all but one gene. Expression differences were greater in HD than I pigs. Significant downregulation for certain immune genes in low pigs, relative to littermate controls, was detected in lung and BLN, particularly in line I. Serum levels of the immune cytokines affirmed the gene expression differences. High preinfection serum levels of IL 8 were significantly associated with PRRS virus-resistant, low pigs. After infection, low expression of interferon gamma in cDNA and in serum was also correlated with PRRS virus resistance. Important genetic associations were revealed for fine mapping of candidate genes for PRRS virus resistance and determining the causative alleles
Recommended from our members
De novo formation of an aggregation pheromone precursor by an isoprenyl diphosphate synthase-related terpene synthase in the harlequin bug.
Insects use a diverse array of specialized terpene metabolites as pheromones in intraspecific interactions. In contrast to plants and microbes, which employ enzymes called terpene synthases (TPSs) to synthesize terpene metabolites, limited information from few species is available about the enzymatic mechanisms underlying terpene pheromone biosynthesis in insects. Several stink bugs (Hemiptera: Pentatomidae), among them severe agricultural pests, release 15-carbon sesquiterpenes with a bisabolene skeleton as sex or aggregation pheromones. The harlequin bug, Murgantia histrionica, a specialist pest of crucifers, uses two stereoisomers of 10,11-epoxy-1-bisabolen-3-ol as a male-released aggregation pheromone called murgantiol. We show that MhTPS (MhIDS-1), an enzyme unrelated to plant and microbial TPSs but with similarity to trans-isoprenyl diphosphate synthases (IDS) of the core terpene biosynthetic pathway, catalyzes the formation of (1S,6S,7R)-1,10-bisaboladien-1-ol (sesquipiperitol) as a terpene intermediate in murgantiol biosynthesis. Sesquipiperitol, a so-far-unknown compound in animals, also occurs in plants, indicating convergent evolution in the biosynthesis of this sesquiterpene. RNAi-mediated knockdown of MhTPS mRNA confirmed the role of MhTPS in murgantiol biosynthesis. MhTPS expression is highly specific to tissues lining the cuticle of the abdominal sternites of mature males. Phylogenetic analysis suggests that MhTPS is derived from a trans-IDS progenitor and diverged from bona fide trans-IDS proteins including MhIDS-2, which functions as an (E,E)-farnesyl diphosphate (FPP) synthase. Structure-guided mutagenesis revealed several residues critical to MhTPS and MhFPPS activity. The emergence of an IDS-like protein with TPS activity in M. histrionica demonstrates that de novo terpene biosynthesis evolved in the Hemiptera in an adaptation for intraspecific communication
Reported variability in healthcare facility policies regarding healthcare personnel working while experiencing influenza-like illnesses: An emerging infections network survey
BACKGROUND: Presenteeism, or working while ill, by healthcare personnel (HCP) experiencing influenza-like illness (ILI) puts patients and coworkers at risk. However, hospital policies and practices may not consistently facilitate HCP staying home when ill.
OBJECTIVE AND METHODS: We conducted a mixed-methods survey in March 2018 of Emerging Infections Network infectious diseases physicians, describing institutional experiences with and policies for HCP working with ILI.
RESULTS: Of 715 physicians, 367 (51%) responded. Of 367, 135 (37%) were unaware of institutional policies. Of the remaining 232 respondents, 206 (89%) reported institutional policies regarding work restrictions for HCP with influenza or ILI, but only 145 (63%) said these were communicated at least annually. More than half of respondents (124, 53%) reported that adherence to work restrictions was not monitored or enforced. Work restrictions were most often not perceived to be enforced for physicians-in-training and attending physicians. Nearly all (223, 96%) reported that their facility tracked laboratory-confirmed influenza (LCI) in patients; 85 (37%) reported tracking ILI. For employees, 109 (47%) reported tracking of LCI and 53 (23%) reported tracking ILI. For independent physicians, not employed by the facility, 30 (13%) reported tracking LCI and 11 (5%) ILI.
CONCLUSION: More than one-third of respondents were unaware of whether their institutions had policies to prevent HCP with ILI from working; among those with knowledge of institutional policies, dissemination, monitoring, and enforcement of these policies was highly variable. Improving communication about work-restriction policies, as well as monitoring and enforcement, may help prevent the spread of infections from HCP to patients
The Parasitic Wasp, Cotesia congregata (Say), Consists of Two Incipient Species Isolated by Asymmetric Reproductive Incompatibility and Hybrid Inability to Overcome Host Defenses
Parasitic wasps are highly diverse and play a major role in suppression of herbivorous insect pest populations. Several previously identified species of parasitic wasps have been found to be complexes of cryptic species resulting from adaptations to specific hosts or host foodplants. Cotesia congregata (Say) (Hymenoptera: Braconidae), which has long served as a model system for host-parasitoid interactions, can be used for investigating the process of diversification among sympatric populations that differ in host and host foodplant usage. Two incipient species of C. congregata have been identified in the USA mid-Atlantic region, “MsT wasps” originate from Manduca sexta (L.) (Lepidoptera: Sphingidae) on tobacco and “CcC wasps” originate from Ceratomia catalpae (Boisduval) (Lepidoptera: Sphingidae) on catalpa. Both wasp sources can develop in either host species. Hybrids resulting from MsT♂xCcC♀ crosses are fertile, whereas hybrids from CcC♂xMsT♀ crosses are typically sterile. In this study, we compared relative expression in vivo of seven C. congregata bracovirus (CcBV) genes among MsT and CcC parental and hybrid crosses. Also, we established hybrid crosses between MsT and CcC wasps and four additional host foodplant sources of C. congregata. Patterns of relative expression in vivo of MsT and CcC CcBV genes differed; a few were not expressed in hosts parasitized by CcC wasps. Overall, relative expression of CcBV genes from MsT and CcC wasps did not differ with respect to the host species parasitized. Low or absent expression of CcBV genes was found in hosts parasitized by sterile hybrids. For the most part, the other four host-foodplant wasp sources were reproductively compatible with either MsT or CcC wasps and hybrid crosses with the alternative wasp source were asymmetrically sterile. Crosses involving CcC males or MsT females produced sterile hybrids that lacked mature ovaries. Cumulatively, results indicate that C. congregata is composed of two sympatric incipient species that can utilize multiple host species rather than several host-associated races or cryptic species
- …