Ironie in die Johaimesevangelie

Irony in the Gospel of JohnAn exponent of literary theory and criticism described the shift of focus from the text to the reader as a revolution. One of the main features of irony is the intensive involvement of the reader. As far as John is concerned, some scholars are convinced that 'In the Fourth Gospel theology is irony'. This may be an exaggeration, but emphasises the need to ascertain to v/hat extent irony is used in John and what contribution the study of irony in John can make to the better understanding of themessage of the Gospel. In order to answer these questions, one must firstdetermine what irony is, how it functions and how it can be detected in a text. This article is an attempt in this direction

Hermanus Magnetic Observatory: a historical perspective of geomagnetism in southern Africa

In this paper a brief summary will be given about the historical development of geomagnetism as a science in southern Africa and particularly the role played by Hermanus Magnetic Observatory in this regard. From a very modest beginning in 1841 as a recording station at the Cape of Good Hope, Hermanus Magnetic Observatory is today part of the South African National Space Agency (SANSA), where its geomagnetic field data are extensively used in international research projects ranging from the physics of the geo-dynamo to studies of the near-Earth space environment.</p

Noodlottige kindermishandeling: ‘n literatuuroorsig en die profiel in die Suid-Vrystaat (1995-2003) Fatal child abuse: a literature review and the profile in the Southern Free State (1995-2003)

Agtergrond Kindermishandeling is \'n sosiale probleem met verreikende gevolge. Ernstige onderrapportering van gevalle vind plaas, en geen studies kon opgespoor word wat die Suid-Afrikaanse konteks van die probleem ondersoek nie. In hierdie studie is gepoog om die profiel van die slagoffers van noodlottige kindermishandeling in die Suid-Vrystaat te ondersoek. Metodes Alle kindersterftes van kinders van tien jaar en jonger is uit die register van sterftes by die SAPD- (Suid-Afrikaanse Polisiediens-) lykshuis, Bloemfontein, geïdentifiseer. Hierdie sterftes se verslae is vir gevalle van noodlottige kindermishandeling geëvalueer. Irrelevante verslae is geëlimineer totdat \'n groep moontlike gevalle van noodlottige kindermishandeling verkry is. In gevalle waar daar twyfel oor die oorsaak van dood was, is \'n kliniese assistent in Geregtelike Geneeskunde geraadpleeg. In sommige gevalle het die assistent aanbeveel dat \'n geskiedenis uit polisieverslae verkry moes word. Indien gevalle nie op so \'n wyse bevestig kon word nie, is dit in die sogenaamde ‘grys area\' ingesluit. Negentien gevalle is geïdentifiseer en vier is in die grys area geplaas. Resultate Uit die 19 gevalle was die meerderheid vroulik (14). Die mediaanouderdom van slagoffers was twee jaar. Die slag-offers toon \'n eweredige verspreiding t.o.v. ras. Geen patroon kon geïdentifiseer word in die jaarlikse en maandelikse verspreiding van sterftes nie. Die meeste van die slagoffers, 73.7% (14 van 19 gevalle), was van normale massa, en geen slagoffers het aan \'n wanvoedingsiekte gely nie. Hoofbese-rings was die algemeenste oorsaak van sterftes (63.2%), gevolg deur veelvuldige beserings (21.2%). Gevolgtrekking Indien dokters bedag is op die tekens van kindermishandeling, kan gevalle vroeg geïdentifiseer word. Sodoende kan \'n beduidende verlaging in mortaliteit en morbiditeit bewerkstellig Introduction Child abuse is a social problem with far-reaching consequences. Serious underreporting of cases occurs, and no studies could be found which investigates the South African context of the problem. In this study we aimed to determine the profile of victims of fatal child abuse in the Southern Free State. Methods All child deaths of children 10 years and younger in the period 1 January 1995 to 31 December 2003 were identified in the register of deaths at the SA Police Mortuary, Bloemfontein. The reports of these deaths were evaluated to identify possible cases of fatal child abuse. Irrelevant reports were eliminated until a group of possible cases of fatal child abuse were identified. In cases where there was uncertainty about the cause of death, a registrar in Forensic Medicine was consulted. In some cases she advised that a history be obtained from police reports. Cases which could not be confirmed in this way were included in a so-called grey area. Nineteen cases were identified, and a further 4 placed in the grey area. Results Of the 19 cases, the majority were female (14). The median age of cases was 2 years. Cases showed an even distribution regarding race. No patterns regarding annual or monthly distribution were found. Most victims, 73.7% (14 of 19 cases), were of normal weight and no victims suffered from malnutrition. Head injuries were the most common cause of death (63.2%) followed by multiple injuries (21.2%). Conclusion If doctors are aware of the signs of child abuse cases can be identified early. In this way a significant decrease in mortality and morbidity can occur. word.South African Family Practice Vol. 49 (9) 2007: pp. 1

Identifying sequence variation in cation channel sperm associated genes in Cape mountain zebra (Equus zebra zebra)

The Cape mountain zebra (Equus zebra zebra) has recovered from near extinction over more than eight decades. While their numbers have increased, populations remain isolated with low genetic diversity. With more than 75 new populations being founded and more than 4800 extant animals, conservation management strategies are being implemented to mitigate risk of losses in genetic diversity and reproductive fitness. One objective is to identify reproductive characteristics that may improve population growth. Cation channel sperm (CatSper) genes play an important role in hyperactivation of sperm during fertilization. Mutations in these genes lead to reduced fertility and even infertility. Ten male zebras were sampled from a group that were translocated in 2016 in order to found a new population. Single nucleotide polymorphisms (SNPs) were identified in three of the CatSper genes (1 - 3). Lack of variation was observed in all exons, with only four SNPs being identified in the intronic regions in close proximity to exons 1, 2, 7, 8, and 9 of CatSper 1. These results may contribute to the pre-identification of males for new founder populations to ensure population growth and viability, and may be a useful tool for selection against low-producing individuals

Uptake and transport of novel amphiphilic polyelectrolyte-insulin nanocomplexes by caco-2 cells - towards oral insulin

“The original publication is available at www.springerlink.com”. Copyright SpringerPurpose: The influence of polymer architecture on cellular uptake and transport across Caco-2 cells of novel amphiphilic polyelectrolyte-insulin nanocomplexes was investigated. Method: Polyallylamine (PAA) (15 kDa) was grafted with palmitoyl chains (Pa) and subsequently modified with quaternary ammonium moieties (QPa). These two amphiphilic polyelectrolytes (APs) were tagged with rhodamine and their uptake by Caco-2 cells or their polyelectrolyte complexes (PECs) with fluorescein isothiocyanate-insulin (FITC-insulin) uptake were investigated using fluorescence microscopy. The integrity of the monolayer was determined by measurement of transepithelial electrical resistance (TEER). Insulin transport through Caco-2 monolayers was determined during TEER experiments. Result: Pa and insulin were co-localised in the cell membranes while QPa complexes were found within the cytoplasm. QPa complex uptake was not affected by calcium, cytochalasin D or nocodazole. Uptake was reduced by co-incubation with sodium azide, an active transport inhibitor. Both polymers opened tight junctions reversibly where the TEER values fell by up to 35 % within 30 minutes incubation with Caco-2 cells. Insulin transport through monolayers increased when QPa was used (0.27 ngmL-1 of insulin in basal compartment) compared to Pa (0.14 ngmL-1 of insulin in basal compartment) after 2 hours. Conclusion: These APs have been shown to be taken up by Caco-2 cells and reversibly open tight cell junctions. Further work is required to optimise these formulations with a view to maximising their potential to facilitate oral delivery of insulin.Peer reviewe

Phyllosticta citricarpa and sister species of global importance to Citrus.

Several Phyllosticta species are known as pathogens of Citrus spp., and are responsible for various disease symptoms including leaf and fruit spots. One of the most important species is P. citricarpa, which causes a foliar and fruit disease called citrus black spot. The Phyllosticta species occurring on citrus can most effectively be distinguished from P. citricarpa by means of multilocus DNA sequence data. Recent studies also demonstrated P. citricarpa to be heterothallic, and reported successful mating in the laboratory. Since the domestication of citrus, different clones of P. citricarpa have escaped Asia to other continents via trade routes, with obvious disease management consequences. This pathogen profile represents a comprehensive literature review of this pathogen and allied taxa associated with citrus, focusing on identification, distribution, genomics, epidemiology and disease management. This review also considers the knowledge emerging from seven genomes of Phyllosticta spp., demonstrating unknown aspects of these species, including their mating behaviour.TaxonomyPhyllosticta citricarpa (McAlpine) Aa, 1973. Kingdom Fungi, Phylum Ascomycota, Class Dothideomycetes, Order Botryosphaeriales, Family Phyllostictaceae, Genus Phyllosticta, Species citricarpa.Host rangeConfirmed on more than 12 Citrus species, Phyllosticta citricarpa has only been found on plant species in the Rutaceae.Disease symptomsP. citricarpa causes diverse symptoms such as hard spot, virulent spot, false melanose and freckle spot on fruit, and necrotic lesions on leaves and twigs.Useful websitesDOE Joint Genome Institute MycoCosm portals for the Phyllosticta capitalensis (https://genome.jgi.doe.gov/Phycap1), P. citriasiana (https://genome.jgi.doe.gov/Phycit1), P. citribraziliensis (https://genome.jgi.doe.gov/Phcit1), P. citrichinaensis (https://genome.jgi.doe.gov/Phcitr1), P. citricarpa (https://genome.jgi.doe.gov/Phycitr1, https://genome.jgi.doe.gov/Phycpc1), P. paracitricarpa (https://genome.jgi.doe.gov/Phy27169) genomes. All available Phyllosticta genomes on MycoCosm can be viewed at https://genome.jgi.doe.gov/Phyllosticta

Biblical Theology of Life in the Old Testament

Life is a primary theme in Scripture, expressed in the rich diversity of the various books, corpora and genres of Scripture. Much has been published on what Scripture teaches about life and death. To date, however, no comprehensive biblical theology in which the concept of life is traced throughout the different books and corpora of the Old and New Testament has been published. It is this lacuna that this book aims to fill, assuming that such an approach can provide a valuable contribution to the theological discourse on life and related concepts. The primary aim of this book is to give an indication of the different nuances of the concept of life in the various books and corpora of the Old and New Testament by providing the reader with a book-by-book overview of the concept of life in Scripture. The secondary aim is to give an indication of the overall use and function of the concept of life in the Old Testament, the New Testament, and Scripture as a whole. The latter is provided by using the findings of the book-by-book overview of the concept of life in Scripture to draw the lines together

[89Zr]Oxinate4 for long-term in vivo cell tracking by positron emission tomography

Purpose 111In (typically as [111In]oxinate3) is a gold standard radiolabel for cell tracking in humans by scintigraphy. A long half-life positron-emitting radiolabel to serve the same purpose using positron emission tomography (PET) has long been sought. We aimed to develop an 89Zr PET tracer for cell labelling and compare it with [111In]oxinate3 single photon emission computed tomography (SPECT). Methods [89Zr]Oxinate4 was synthesised and its uptake and efflux were measured in vitro in three cell lines and in human leukocytes. The in vivo biodistribution of eGFP-5T33 murine myeloma cells labelled using [89Zr]oxinate4 or [111In]oxinate3 was monitored for up to 14 days. 89Zr retention by living radiolabelled eGFP-positive cells in vivo was monitored by FACS sorting of liver, spleen and bone marrow cells followed by gamma counting. Results Zr labelling was effective in all cell types with yields comparable with 111In labelling. Retention of 89Zr in cells in vitro after 24 h was significantly better (range 71 to >90 %) than 111In (43–52 %). eGFP-5T33 cells in vivo showed the same early biodistribution whether labelled with 111In or 89Zr (initial pulmonary accumulation followed by migration to liver, spleen and bone marrow), but later translocation of radioactivity to kidneys was much greater for 111In. In liver, spleen and bone marrow at least 92 % of 89Zr remained associated with eGFP-positive cells after 7 days in vivo. Conclusion [89Zr]Oxinate4 offers a potential solution to the emerging need for a long half-life PET tracer for cell tracking in vivo and deserves further evaluation of its effects on survival and behaviour of different cell types

Biblical Theology of Life in the Old Testament

Life is a primary theme in Scripture, expressed in the rich diversity of the various books, corpora and genres of Scripture. Much has been published on what Scripture teaches about life and death. To date, however, no comprehensive biblical theology in which the concept of life is traced throughout the different books and corpora of the Old and New Testament has been published. It is this lacuna that this book aims to fill, assuming that such an approach can provide a valuable contribution to the theological discourse on life and related concepts. The primary aim of this book is to give an indication of the different nuances of the concept of life in the various books and corpora of the Old and New Testament by providing the reader with a book-by-book overview of the concept of life in Scripture. The secondary aim is to give an indication of the overall use and function of the concept of life in the Old Testament, the New Testament, and Scripture as a whole. The latter is provided by using the findings of the book-by-book overview of the concept of life in Scripture to draw the lines together

A critique of the Global Pact for the Environment: A stillborn initiative or the foundation for Lex Anthropocenae?

In May 2018 the process which may ultimately lead to the negotiation of a legally binding Global Pact for the Environment formally commenced under the auspices of the United Nations General Assembly. Expectations for the Pact are high, evidenced in particular by its multiple and overlapping objectives: to serve as a generic binding instrument of international environmental law (IEL) principles ; to integrate, consolidate, unify and ultimately entrench many of the fragmented principles of IEL; and to constitute the first global environmental human rights instrument. In the wake of the impending intergovernmental process, the paper offers a thorough critique of the draft Pact in its present iteration. We do so with the aim of evaluating the strengths and weaknesses of the present draft Pact by interrogating: a) its diplomatic and symbolic relevance and possible unique contribution at the policy level to global environmental law and governance; and b) its potential at the operational level of IEL and global environmental governance, focusing on the extent to which the draft Pact accommodates both existing and more recent rules and principles for environmental protection. As the Pact’s primary ambition is to become a universally binding global treaty, it would be churlish not to recognise its potential for innovation, as well as the considerable opportunity that the negotiation of the Pact will have to generate broad-sweeping and positive impacts. However, our central thesis is that only if the Global Pact were to incorporate ambitious normative provisions to strengthen those public and private global governance efforts that aim to halt the deterioration of Earth system integrity, as well as to maintain and improve integrity, will it be able to offer a firm foundation of the type of Anthropocene Law, termed here as the Lex Anthropocenae, required to confront head-on the deep socio-ecological crisis of the Anthropocene