Conference highlights of the 15th international conference on human retrovirology: HTLV and related retroviruses, 4-8 june 2011, Leuven, Gembloux, Belgium

The June 2011 15th International Conference on Human Retrovirology: HTLV and Related Viruses marks approximately 30 years since the discovery of HTLV-1. As anticipated, a large number of abstracts were submitted and presented by scientists, new and old to the field of retrovirology, from all five continents. The aim of this review is to distribute the scientific highlights of the presentations as analysed and represented by experts in specific fields of epidemiology, clinical research, immunology, animal models, molecular and cellular biology, and virology

Human T lymphotropic virus type 1 (HTLV-1) regulatory and accessory gene transcript expression and export are not Rex-dependent

Human T lymphotropic virus type 1 (HTLV-1) requires regulated gene expression from unspliced and alternatively spliced transcripts for efficient replication and persistence. HTLV-1 Rex is known to facilitate cytoplasmic export of unspliced, gag/pol and incompletely spliced env mRNAs, but its contribution to the expression of other viral transcripts has not been experimentally assessed. In this study, we utilized HTLV-1 proviral clones, cellular fractionation, and real-time reverse transcriptase PCR to determine the role of Rex on the expression and export of all viral mRNAs. Our results indicate that the steady-state levels of the different viral mRNAs are modulated by Rex, which we attribute to a redistribution of completely spliced mRNAs toward incompletely spliced mRNAs. Furthermore, we confirmed the positive effect of Rex on the unspliced gag/pol mRNA and singly spliced env mRNA, resulting in increased cytoplasmic expression. However, the cytoplasmic export of the alternatively spliced HTLV-1 mRNAs encoding the accessory proteins and the antisense Hbz mRNA are independent of direct Rex regulation. This is consistent with the conclusion that viral mRNAs that contain the cis-acting repressive sequence (CRS) and/or a fully functional splice donor site require a Rex/RxRE interaction for efficient cytoplasmic expression