Use of a distant reporter group as evidence for a conformational change in a sensory receptor

A highly sensitive method for demonstrating ligand-induced conformational changes in protein molecules in solution is described. The method utilizes an environmentally sensitive reporter group that is known to be distant from the active site. In the present application a conformational change is demonstrated in the galactose receptor of Salmonella typhimurium, involved in bacterial sensing and transport, by means of an extrinsic fluorophore, 5-iodoacetamidofluorescein, attached at a single methionine residue, and the intrinsic tryptophan fluorophore. Binding of the ligand galactose perturbs the microenvironment of both the fluorescein and tryptophan, as shown by both spectral and potassium iodide quenching changes. The distance between the two dyes is established by fluorescence energy transfer methods to be 41 ± 10 angstrom. Since only one molecule of galactose binds per molecule of receptor and since the galactose molecule is only about 5 angstrom in length, changes at one of these sites reflect the result of an indirect effect. Hence, there must be a ligand-induced conformational change that is propagated a minimum of 30 angstrom through the receptor molecule

The Ultraviolet Emission Properties of Five Low-Redshift Active Galactic Nuclei at High Signal to Noise and Spectral Resolution

We analyze the ultraviolet (UV) emission line and continuum properties of five low-redshift active galactic nuclei (four luminous quasars: PKS~0405$-$123, H1821+643, PG~0953+414, and 3C273, and one bright Seyfert 1 galaxy: Mrk~205). The HST spectra have higher signal-to-noise ratios (typically $\sim 60$ per resolution element) and spectral resolution ($R = 1300$) than all previously- published UV spectra used to study the emission characteristics of active galactic nuclei. We include in the analysis ground-based optical spectra covering \hb\ and the narrow [O III] $\lambda\lambda$4959,5007 doublet. The following new results are obtained: \lyb/\lya=0.03$-$0.12 for the four quasars, which is the first accurate measurement of the long-predicted \lyb\ intensity in QSOs. The cores of \lya\ and C~IV are symmetric to an accuracy of better than 2.5% within about 2000 km s$^{-1}$ of the line peak. This high degree of symmetry of \lya\ argues against models in which the broad line cloud velocity field has a significant radial component. The observed smoothness of the \lya\ and C~IV line profiles requires at least $\sim 10^4$ individual clouds if bulk velocity is the only line-broadening mechanism. The overall similarity of the \lya\ and C IV $\lambda$1549 profiles rules out models for the broad line region (BLR) with a radial distribution of virialized....Comment: 39 pages (+ 6 pages of tables + 16 of figures), AST 93/2

Rocket and FUSE Observations of IC 405: Differential Extinction and Fluorescent Molecular Hydrogen

We present far-ultraviolet spectroscopy of the emission/reflection nebula IC 405 obtained by a rocket-borne long-slit spectrograph and the Far Ultraviolet Spectroscopic Explorer. Both data sets show a rise in the ratio of the nebular surface brightness to stellar flux (S/F_*) of approximately two orders of magnitude towards the blue end of the far-UV bandpass. Scattering models using simple dust geometries fail to reproduce the observed S/F_* for realistic grain properties. The high spectral resolution of the FUSE data reveals a rich fluorescent molecular hydrogen spectrum ~1000" north of the star that is clearly distinguished from the steady blue continuum. The S/F_* remains roughly constant at all nebular pointings, showing that fluorescent molecular hydrogen is not the dominant cause for the blue rise. We discuss three possible mechanisms for the ``Blue Dust'': differential extinction of the dominant star (HD 34078), unusual dust grain properties, and emission from nebular dust. We conclude that uncertainties in the nebular geometry and the degree of dust clumping are most likely responsible for the blue rise. As an interesting consequence of this result, we consider how IC 405 would appear in a spatially unresolved observation. If IC 405 was observed with a spatial resolution of less than 0.4 pc, for example, an observer would infer a far-UV flux that was 2.5 times the true value, giving the appearance of a stellar continuum that was less extinguished than radiation from the surrounding nebula, an effect that is reminiscent of the observed ultraviolet properties of starburst galaxies.Comment: 8 pages, 9 figures, using emulateapj, ApJ - accepte

East Asian audio-visual collaboration and the global expansion of Chinese media

This article investigates the significant re-orientation of audio-visual production in East Asia over the last few years brought about by the rise of China, beginning with the proposition that unprecedented change is occurring in East Asian media production. While the 'Sinophone world' has been the locus of critical analysis in the past, all eyes are now focused on China. Flows of knowledge, expertise and content are becoming significant in this mediascape, yet this dimension has been overlooked by most scholarship in the field. Conceptual and theoretical frameworks based on cross-border consumption of East Asian content require urgent revision. This article shows how media collaborations are changing global media practice and East Asian media flows through a variety of contemporary international collaborations, as well as relevant policy frameworks that impact, positively or negatively, productions by international partners working in film, television and online and mobile video content

Targeted colonic release formulations of mesalazine - A clinical pharmaco-scintigraphic proof-of-concept study in healthy subjects and patients with mildly active ulcerative colitis.

Colonic targeting of orally applied therapeutic drugs remains a challenge. Tablet coatings relying on gastrointestinal pH and colonic bacterial enzymes as triggers in association with an inner alkaline layer are expected to improve targeting efficiency. Mesalazine release from three differently coated tablets labelled with 1 MBq 153Sm was characterised in a single centre, open-label, parallel group study in nineteen healthy subjects and seven patients with mildly active ulcerative colitis. Two semi-organic and one aqueous-based outer coating with different ratios of enteric polymer and resistant starch were tested. All coatings showed comparable release lagtimes in biorelevant dissolution media and were not affected by neutron-activation of the samarium tracer. Mesalazine pharmacokinetics and gamma scintigraphy were used to characterise drug release, anatomical site of tablet disintegration and gastrointestinal transit. Initial tablet disintegration occurred at the ileo-caecal junction or beyond in 92% of the subjects Time to initial tablet disintegration was inversely correlated with maximal plasma concentrations and systemic mesalazine exposure. Although high inter-subject variability precluded detection of differences between solvent types and different enteric polymer to polysaccharide ratios, the dual pH and enzymatic triggered release system in combination with an inner alkaline layer promoted mesalazine release at the target site with high accuracy

Complete Hydrogen Storage System by ISRU

New technologies make it possible to build in space a complete hydrogen storage system using ISRU methods and techniques. Hydrogen can be stored in a solid-state form on the surface atoms of high surface area matrices such as those of porous silicon. Silicon is abundant in regolith and can be purified using a purely mechanical means which results in particulates in the scale range of tens of nanometers. Reagents used to porosify these nano-particles can be regenerated thermally to essentially eliminate the need for resupply from earth. Catalysts are needed to divide dihydrogen gas into atomic hydrogen for solid-state adsorption and to mediate the temperatures and pressures of charge and discharge into ranges easily achievable with simple equipment. Recent research has identified the utility of non-platinum group catalyst materials which are widespread on the moon. Rapid discharge, needed for propulsion, is possible with infra-red illumination at wavelengths which pass through pure silicon but are absorbed by the silicon-hydrogen bond. Such IR emitters can be fabricated by embossing of silica and additive manufacturing of metals. Control and power electronics can be fabricated using a patented process designed for space operations, and built on either silicon or silicon carbide substrates derived from regolith. Bringing these five technologies together for the first time allows a system which can be fed with moderate pressure gaseous hydrogen at moderate temperatures, stored for long durations with minimum loss, then released upon demand across a wide range of controllable rates. Such a system can displace the need for cryogenic hydrogen storage. Being suitable to bottom-up fabrication using only in-space materials makes this a “green” ISRU technology to store hydrogen for fuel cells, rocket engines, and chemical processes

HST/ACS weak lensing analysis of the galaxy cluster RDCS 1252.9-2927 at z=1.24

We present a weak lensing analysis of one of the most distant massive galaxy cluster known, RDCS 1252.9-2927 at z=1.24, using deep images from the Advanced Camera for Survey (ACS) on board the Hubble Space Telescope (HST). By taking advantage of the depth and of the angular resolution of the ACS images, we detect for the first time at z>1 a clear weak lensing signal in both the i (F775W) and z (F850LP) filters. We measure a 5-\sigma signal in the i band and a 3-\sigma signal in the shallower z band image. The two radial mass profiles are found to be in very good agreement with each other, and provide a measurement of the total mass of the cluster inside a 1Mpc radius of M(<1Mpc) = (8.0 +/- 1.3) x 10^14 M_\odot in the current cosmological concordance model h =0.70, \Omega_m=0.3, \Omega_\Lambda=0.7, assuming a redshift distribution of background galaxies as inferred from the Hubble Deep Fields surveys. A weak lensing signal is detected out to the boundary of our field (3' radius, corresponding to 1.5Mpc at the cluster redshift). We detect a small offset between the centroid of the weak lensing mass map and the brightest cluster galaxy, and we discuss the possible origin of this discrepancy. The cumulative weak lensing radial mass profile is found to be in good agreement with the X-ray mass estimate based on Chandr and XMM-Newton observations, at least out to R_500=0.5Mpc.Comment: 38 pages, ApJ in press. Full resolution images available at http://www.eso.org/~prosati/RDCS1252/Lombardi_etal_accepted.pd

VLT and ACS observations of RDCS J1252.9-2927: dynamical structure and galaxy populations in a massive cluster at z=1.237

We present results from an extensive spectroscopic survey, carried out with VLT FORS, and from an extensive multiwavelength imaging data set from the HST Advanced Camera for Surveys and ground based facilities, of the cluster of galaxies RDCS J1252.9-2927. We have spectroscopically confirmed 38 cluster members in the redshift range 1.22 < z < 1.25. A cluster median redshift of z=1.237 and a rest-frame velocity dispersion of 747^{+74}_{-84} km/s are obtained. Using the 38 confirmed redshifts, we were able to resolve, for the first time at z > 1, kinematic structure. The velocity distribution, which is not Gaussian at the 95% confidence level, is consistent with two groups that are also responsible for the projected east-west elongation of the cluster. The groups are composed of 26 and 12 galaxies with velocity dispersions of 486^{+47}_{-85} km/s and 426^{+57}_{-105} km/s, respectively. The elongation is also seen in the intracluster gas and the dark matter distribution. This leads us to conclude that RDCS J1252.9-2927 has not yet reached a final virial state. We extend the analysis of the color-magnitude diagram of spectroscopic members to more than 1 Mpc from the cluster center. The scatter and slope of non-[OII]-emitting cluster members in the near-IR red sequence is similar to that seen in clusters at lower redshift. Furthermore, most of the galaxies with luminosities greater than ~ K_s*+1.5 do not show any [OII], indicating that these more luminous, redder galaxies have stopped forming stars earlier than the fainter, bluer galaxies. Our observations provide detailed dynamical and spectrophotometric information on galaxies in this exceptional high-redshift cluster, delivering an in-depth view of structure formation at this epoch only 5 Gyr after the Big Bang.Comment: 29 pages. 16 figures. ApJ accepted. Tables 2,3 and 5, figure 1 and the full figure 5 will be available in the paper and electronic editions from ApJ. v2: minor corrections to the abstract and text to match the Journal's versio

Male Competition Reverses Female Preference For Male Chemical Cues

Females must choose among potential mates with different phenotypes in a variety of social contexts. Many male traits are inherent and unchanging, but others are labile to social context. Competition, for example, can cause physiological changes that reflect recent wins and losses that fluctuate throughout time. We may expect females to respond differently to males depending on the outcome of their most recent fight. In Bolitotherus cornutus (forked fungus beetles), males compete for access to females, but copulation requires female cooperation. In this study, we use behavioral trials to determine whether females use chemical cues to differentiate between males and whether the outcome of recent male competition alters female preference. We measured female association time with chemical cues of two size‐matched males both before and after male–male competition. Females in our study preferred to associate with future losers before males interacted, but changed their preference for realized winners following male competitive interactions. Our study provides the first evidence of change in female preference based solely on the outcome of male–male competition