Molecular detection of parasites (Trematoda, Digenea: Bucephalidae and Monorchiidae) in the European flat oyster Ostrea edulis (Mollusca: Bivalvia)

Members of the globally distributed bivalve family Ostreidae (oysters) have a significant role in marine ecosystems and include species of high economic importance. In this work, we report the occurrence of digenean parasites of the families Bucephalidae (Prosorhynchoides sp.) and Monorchiidae (Postmonorchis sp.) in Mediterranean native populations of Ostrea edulis (but not in the introduced Magallana gigas). Molecular detection was based on DNA sequencing of the ribosomal intergenic spacer 2 (ITS2) marker. The importance of detecting the presence of overlooked digenean parasites in Mediterranean oysters is discussed. © 2018 The Author(s). Published by Informa UK Limited, trading as Taylor & Francis Group

Inhibition of acetylpolyamine and spermine oxidases by the polyamine analogue chlorhexidine

""Acetylpolyamine and spermine oxidases are involved in the catabolism of polyamines. The discovery of selective. inhibitors of these enzymes represents an important tool for the development of novel anti-neoplastic drugs. Here, a. comparative study on acetylpolyamine and spermine oxidases inhibition by the polyamine analogue chlorhexidine. is reported. Chlorhexidine is an antiseptic diamide, commonly used as a bactericidal and bacteriostatic agent.. Docking simulations indicate that chlorhexidine binding to these enzymes is compatible with the stereochemical. properties of both acetylpolyamine oxidase and spermine oxidase active sites. In fact, chlorhexidine is predicted. to establish several polar and hydrophobic interactions with the active site residues of both enzymes, with binding. energy values ranging from −7.6 to −10.6 kcal\\\/mol. In agreement with this hypothesis, inhibition studies indicate that. chlorhexidine behaves as a strong competitive inhibitor of both enzymes, values of Ki being 0.10 μM and 0.55 μM for. acetylpolyamine oxidase and spermine oxidase, respectively."

A 1.25 Gbit/s serializer for LHC data and trigger optical links

Several LHC detectors require high-speed digital optical links for data transmission in both data readout and trigger systems. Commercial components can be found that meet the bandwidth requirements of most of the LHC detectors subsystems. However, they fail to meet some of the requirements frequently encountered in the LHC-HEP environment, namely: resistance to high radiation doses and operation tolerant to single event upsets. To address these problems, a high-speed transmitter ASIC (1.2Gbit/s), containing a serializer and a clock multiplying PLL was developed. The prototype was implemented in a mainstream 0.25um CMOS technology and was designed using well-established radiation tolerant layout practices to achieve resistance to high radiation doses. This implementation serves as a base for the development of radiation tolerant IC’s that will make feasible the transmission of data using common local area networks protocols in typical LHC radiation hard environments. The ASIC was embedded in a test setup that uses a commercial optical receiver and de-serializer. Error free data transmission at 1.2Gbit/s was achieved proving the prototypes to be fully functional. 1

The Met80Ala and Tyr67His/Met80Ala mutants of human cytochrome cshed light on the reciprocal role of Met80 and Tyr67 in regulating ligand access into the heme pocket.

The spectroscopic and functional properties of the single Met80Ala and double Tyr67His/Met80Ala mutants of human cytochrome c have been investigated in their ferric and ferrous forms, and in the presence of different ligands, in order to clarify the reciprocal effect of these two residues in regulating the access of exogenous molecules into the heme pocket. In the ferric state, both mutants display an aquo high spin and a low spin species. The latter corresponds to an OH- ligand in Met80Ala but to a His in the double mutant. The existence of these two species is also reflected in the functional behavior of the mutants. The observation that (i) a significant peroxidase activity is present in the Met80Ala mutants, (ii) the substitution of the Tyr67 by His leads to only a slight increase of the peroxidase activity in the Tyr67His/Met80Ala double mutant with respect to wild type, while the Tyr67His mutant behaves as wild type, as previously reported, suggests that the peroxidase activity of cytochrome c is linked to an overall conformational change of the heme pocket and not only to the disappearance of the Fe-Met80 bond. Therefore, in human cytochrome c there is an interplay between the two residues at positions 67 and 80 that affects the conformation of the distal side of the heme pocket, and thus the sixth coordination of the hem

Analysis of a gyroscopic-stabilized floating offshore hybrid wind-wave platform

The energy innovation scenario sees hybrid wind-wave platforms as a promising technology for reducing the variability of the power output and for the minimization of the cost of offshore marine renewable installations. This article presents a model that describes the installation of a 5 MW wind turbine on a floating platform designed by Fincantieri and equipped with gyroscopic stabilization. The use of gyros allows for the delivery of platform stabilization by damping the wave and wind induced motion on the floater and at the same time producing extra power. Shetland Island was chosen as the reference site because of its particularly harsh weather. Final results show that the total production of power in moderate and medium climate conditions is considerable thanks to the installation of the gyro, together with a significant stabilization of the platform in terms of pitching angle and nacelle acceleration