High-throughput wide dynamic range procedure for the simultaneous quantification of nicotine and cotinine in multiple biological matrices using hydrophilic interaction liquid chromatography-tandem mass spectrometry

A straightforward, high-throughput method was developed and fully validated for the simultaneous determination of the specific tobacco biomarkers nicotine and its main metabolite cotinine in a wide dynamic range and supporting the most common human biological matrices (urine, oral fluid and hair). Sample preparation was performed inside the very HPLC injection vials by pipetting 0.5 mL of the liquid samples, deuterated internal standards in alkaline solution and dichloromethane as extraction solvent. Solid samples (i.e. around 10 mg hair) were first submitted to alkaline digestion in the HPLC vials and processed accordingly. The organic phase (reached through the upper aqueous layer) was directly injected without further treatment. Instrumental analysis was performed using hydrophilic interaction (HILIC) ultra-high performance liquid chromatography-tandem mass spectrometry (UHPLC-MS/MS). Total chromatographic time was 2 min. The method covers a wide dynamic range making it fit-for-purpose for the analysis of samples covering entire populations, irrespective of the level of exposure or tobacco use. Calibration curves (r 2 > 0.995) covered the range 1-2000 ng/mL (or 0.05-100 ng/mg hair) for nicotine and 0.1-2000 ng/mL (or 0.005-100 ng/mg hair) for cotinine. Within-run and between-run precision and accuracy were typically below 10 %, and always below 20 % at the lower limit of quantification. The method was successfully applied to the analysis of samples from different projects involving multiple matrices

Avances en el estudio de fallas activas, terremotos y peligrosidad sísmica de Iberia.

Depto. de Mineralogía y PetrologíaFac. de Ciencias GeológicasTRUEpu

Passive exposure to electronic cigarette aerosol in pregnancy: A case study of a family

Background Passive exposure to the aerosols of electronic cigarettes (e-cigarettes) has been little studied. We assessed this exposure in late pregnancy in a woman and her 3-year-old child, exposed through e-cigarette use by another household member

Variation in Nicotine Metabolization According to Biological Factors and Type of Nicotine Consumer

This study aims to describe the nicotine metabolite ratio among tobacco smokers and electronic cigarette (e-cigarette) users and nonusers. We analyzed pooled data from a longitudinal and a cross-sectional study of the adult population from the city of Barcelona. The final sample included information on 166 smokers, 164 e-cigarettes users with nicotine, 41 e-cigarette users without nicotine, 95 dual users (users of both products), and 508 nonusers. We used log-linear models to control for the potential confounding effect of the daily number of cigarettes smoked. Salivary nicotine metabolic rate assessment included the rate of nicotine metabolism (cotinine/nicotine) and the nicotine metabolite ratio (trans-3′-hydroxycotinine/cotinine). Exclusive users of e-cigarette without nicotine have the lowest rate of nicotine metabolism (Geometric mean: 0.08, p-values < 0.001) while cigarette smokers have the highest (Geometric mean: 2.08, p-values < 0.001). Nonusers have lower nicotine metabolic rate than cigarette smokers (Geometric means: 0.23 vs. 0.18, p-value < 0.05). Younger individuals (18–44 years) have a higher rate of nicotine metabolism than older individuals (45–64 years and 65–89) (Geometric means: 0.53 vs. 0.42 and 0.31, respectively, p-values < 0.01) and individuals with lower body mass index (21–25 kg/m2) have a higher rate of nicotine metabolism than the rest (26–30 kg/m2 and 31–60 kg/m2) (Geometric means: 0.52 vs. 0.35 and 0.36, respectively-values < 0.01). Nicotine metabolic rates are useful biomarkers when reporting smoking status and biological differences between individuals

Red de seguimiento e indicadores de calidad de la titulación máster universitario en prevención de riesgos laborales

El profesorado de la red participó durante el curso 2013/14 en un proyecto para el seguimiento e indicadores de calidad de la titulación máster universitario en prevención de riesgos laborales. Este Máster comenzó a impartirse en la Escuela Politécnica Superior de la Universidad de Alicante en el curso 2012/13. En el curso 2015/16 al Máster en prevención de riesgos laborales le corresponde la renovación de su acreditación, en base a los resultados de la docencia de los cursos 2013/14 y 2014/15, por lo que los resultados del presente proyecto son de interés para la renovación de la acreditación de la titulación

Renovación de la acreditación de la titulación Máster Universitario en Prevención de Riesgos laborales

El Máster universitario en prevención de riesgos laborales comenzó a impartirse en la Escuela Politécnica Superior de la Universidad de Alicante en el curso 2012/13. En el curso 2015/16 se desarrollara la cuarta edición de esta titulación. El Real Decreto 1393/2007, de 29 de octubre, por el que se establece la ordenación de las enseñanzas universitarias oficiales, modificado por el Real Decreto 861/2010, de 2 de julio, señala en el artículo 27 que, una vez iniciada la implantación de las enseñanzas, la ANECA, o los órganos de evaluación que la ley de las comunidades autónomas determinen, llevarán a cabo el seguimiento del cumplimiento del proyecto contenido en el plan de estudios. Al Máster universitario en prevención de riesgos laborales le corresponde en el curso 2015/16 la renovación de su acreditación, en base a los resultados de la docencia de los cursos 2013/14 y 2014/15. Este proyecto se centra en la preparación de los datos necesarios para el informe de autoevaluación, que será necesario presentar para la evaluación externa de la renovación de la acreditación de la titulación

Impact of the Spanish smoke-free legislation on adult, non-smoker exposure to secondhand smoke: cross-sectional surveys before (2004) and after (2012) legislation

Background: in 2006, Spain implemented a national smoke-free legislation that prohibited smoking in enclosed public places and workplaces (except in hospitality venues). In 2011, it was extended to all hospitality venues and selected outdoor areas (hospital campuses, educational centers, and playgrounds). The objective of the study is to evaluate changes in exposure to secondhand smoke among the adult non-smoking population before the first law (2004-05) and after the second law (2011-12). Methods: repeated cross-sectional survey (2004-2005 and 2011-2012) of a representative sample of the adult (≥16 years) non-smoking population in Barcelona, Spain. We assess self-reported exposure to secondhand smoke (at home, the workplace, during leisure time, and in public/private transportation vehicles) and salivary cotinine concentration. Results: overall, the self-reported exposure to secondhand smoke fell from 75.7% (95%CI: 72.6 to 78.8) in 2004-05 to 56.7% (95%CI: 53.4 to 60.0) in 2011-12. Self-reported exposure decreased from 32.5% to 27.6% (−15.1%, p<0.05) in the home, from 42.9% to 37.5% (−12.6%, p = 0.11) at work/education venues, from 61.3% to 38.9% (−36.5%, p<0.001) during leisure time, and from 12.3% to 3.7% (−69.9%, p<0.001) in public transportation vehicles. Overall, the geometric mean of the salivary cotinine concentration in adult non-smokers fell by 87.2%, from 0.93 ng/mL at baseline to 0.12 ng/mL after legislation (p<0.001). Conclusions: secondhand smoke exposure among non-smokers, assessed both by self-reported exposure and salivary cotinine concentration, decreased after the implementation of a stepwise, comprehensive smoke-free legislation. There was a high reduction in secondhand smoke exposure during leisure time and no displacement of secondhand smoke exposure at home

Exposure to secondhand aerosol from electronic cigarettes at homes: A real-life study in four European countries

Electronic cigarette (e-cigarette) use emits potentially hazardous compounds and deteriorates indoor air quality. Home is a place where e-cigarettes may frequently be used amid its increasing prohibition in public places. This study assessed the real-life scenario of bystanders' exposure to secondhand e-cigarette aerosol (SHA) at home. A one-week observational study was conducted within the TackSHS project in four countries (Greece, Italy, Spain, and the United Kingdom) in 2019 including: 1) homes of e-cigarette users living together with a non-user/non-smoker; and 2) control homes with no smokers nor e-cigarette users. Indoor airborne nicotine, PM2.5, and PM1.0 concentrations were measured as environmental markers of SHA. Biomarkers, including nicotine and its metabolites, tobacco -specific nitrosamines, propanediol, glycerol, and metals were measured in participants' saliva and urine samples. E-cigarette use characteristics, such as e-cigarette refill liquid's nicotine concentration, e-cigarette type, place of e-cigarette use at home, and frequency of ventilation, were also collected. A total of 29 e-cigarette users' homes and 21 control homes were included. The results showed that the seven-day concentrations of airborne nicotine were quantifiable in 21 (72.4 %) out of 29 e-cigarette users' homes; overall, they were quite low (geometric mean: 0.01 mu g/m3; 95 % CI: 0.01-0.02 mu g/m(3)) and were all below the limit of quantification in control homes. Seven-day concentrations of PM2.5 and PM1.0 in e-cigarette and control homes were similar. Airborne nicotine and PM concentrations did not differ according to different e-cigarette use characteristics. Non-users residing with e-cigarette users had low but significantly higher levels of cotinine, 3 '-OH-cotinine and 1,2-propanediol in saliva, and cobalt in urine than non-users living in control homes. In conclusion, e-cigarette use at home created bystanders' exposure to SHA regardless of the e-cigarette use characteristics. Further studies are warranted to assess the implications of SHA exposure for smoke-free policy

New evidences of historical surface rupture on the Alhama de Murcia fault: implications for seismic hazard

Reunión Ibérica sobre Fallas Activas y Paleosismología (3ª. 2018. Alicante). - Texto en inglés con resumen y palabras claves en inglés y españolThe date of the last large event occurred in a fault segment is of great importance in seismic hazard assessment, especially when time-dependent models are considered. Eight of the twelve destructive earthquakes occurred in the eastern Betic Cordillera since 16th century, are located less than 10 km away from the Alhama de Murcia fault (AMF). Up to now, ground surface geological evidence to associate these events with the activity of specific fault segments has not been identified. In this work we present the first geological evidence of the catastrophic 1674 event occurred at Lorca (SE Spain). The excavations at La Torrecilla creek exposed archaeological remains from the Islamic period affected by 55+/-20 cm offset by the AMF fault. This supports the occurrence of earthquakes with surface rupture in the historical period on the Alhama de Murcia Fault and reinforces the results obtained in the previous paleoseismological works. The theoretical scenarios of maximum magnitudes and recurrence time obtained by combining this historical event with the fault slip rate, given the accumulated slip deficit, support that the seismic hazard associated with maximum magnitude events in this section of AMF could be greater than previously thought = La edad del último gran terremoto en un segmento de una falla sismogénica es un parámetro clave para los cálculos de peligrosidad sísmica, especialmente en los cálculos basados en modelos dependientes del tiempo. Ocho de los doce terremotos destructivos ocurridos en las cordilleras Béticas orientales desde el siglo XVI están localizados a menos de 10 km de la traza superficial de la Falla de Alhama de Murcia (AMF). Hasta ahora no se han identificado evidencias geológicas de ruptura superficial asociada a estos eventos que permitan asociarlos a segmentos específicos de la AMF. En este trabajo se presentan evidencias geológicas de ruptura superficial asociada al terremoto de Lorca de 1674. Las excavaciones paleosísmicas realizadas en la rambla de La Torrecilla han expuesto restos de una acequia datada como del periodo árabe que aparece afectada por un desplazamiento de 55+/-20 cm de la falla AMF. Esto apoya la ocurrencia de eventos con ruptura superficial en época histórica a lo largo de la AMF y refuerza las observaciones obtenidas en estudios paleosísmicos previos. Los escenarios teóricos de eventos de magnitud máxima e intervalos de recurrencia, combinando este evento histórico con los valores de tasa de desplazamiento de la AMF disponibles, considerando el déficit de desplazamiento acumulado, apoyan que la peligrosidad sísmica asociada a eventos de magnitud máxima en esta sección de la AMF puede ser mayor de la considerada hasta ahora.Departamento de Geodinámica, Estratigrafía y Paleontología, Universidad Complutense de Madrid, EspañaInstituto de Geociencias, Consejo Superior de Investigaciones Científicas, EspañaInstituto de Geociencias, Universidad Complutense de Madrid, EspañaDepartamento de Geología y Geoquímica, Universidad Autónoma de Madrid, EspañaInstituto Geológico y Minero de España, EspañaDepartamento de Ingeniería Topográfica y Cartografía, Universidad Politécnica de Madrid, EspañaGrupo RISKNAT, Departament de Dinàmica de la Terra i de l’Oceà, Universitat de Barcelona, EspañaAIA Estudio, Españ

Red de coordinación y seguimiento de trabajos fin de máster en la titulación Máster en Prevención de Riesgos Laborales

El profesorado de la red participó durante el curso 2014/15 un proyecto para la coordinación y seguimiento de la asignatura trabajo fin de máster en la titulación Máster en Prevención de Riesgos Laborales. Este Máster comenzó a impartirse en la Escuela Politécnica Superior de la Universidad de Alicante en el curso 2012/13, por ello el desarrollo de la titulación en su tercera edición hace necesario el seguimiento de las asignaturas como instrumento para la coordinación y mejora de la calidad docente. En este sentido la red ha resultado ser un instrumento útil para la interacción y coordinación entre los tutores/as de los trabajos fin de máster propuestos en la titulación