Persistent activation of NF-kappaB related to IkappaB's degradation profiles during early chemical hepatocarcinogenesis

BACKGROUND: To define the NF-kappaB activation in early stages of hepatocarcinogenesis and its IkappaB's degradation profiles in comparison to sole liver regeneration. METHODS: Western-blot and EMSA analyses were performed for the NF-kappaB activation. The transcriptional activity of NF-kappaB was determined by RT-PCR of the IkappaB-α mRNA. The IkappaB's degradation proteins were determined by Western-blot assay. RESULTS: We demonstrated the persistent activation of NF-kappaB during early stages of hepatocarcinogenesis, which reached maximal level 30 min after partial hepatectomy. The DNA binding and transcriptional activity of NF-kappaB, were sustained during early steps of hepatocarcinogenesis in comparison to only partial hepatectomy, which displayed a transitory NF-kappaB activation. In early stages of hepatocarconogenesis, the IkappaB-α degradation turned out to be acute and transitory, but the low levels of IkappaB-β persisted even 15 days after partial hepatectomy. Interestingly, IkappaB-β degradation is not induced after sole partial hepatectomy. CONCLUSION: We propose that during liver regeneration, the transitory stimulation of the transcription factor response, assures blockade of NF-kappaB until recovery of the total mass of the liver and the persistent NF-kappaB activation in early hepatocarcinogenesis may be due to IkappaB-β and IkappaB-α degradation, mainly IkappaB-β degradation, which contributes to gene transcription related to proliferation required for neoplasic progression

Bluetongue in white-tailed deer (Odocoileus virginianus) in Northeastern Mexico

Abstract Bluetongue (BT) and epizootic hemorrhagic disease of deer (EHD) are two distinct viral hemorrhagic diseases of domestic and wild ruminants caused by members of the family Reoviridae and transmitted by Culicoides midges. These conditions have been recognized in Canada and the United States for many years, but not in Mexico. Although in Mexico there is serologic evidence of EHD virus (EHDV) and bluetongue virus (BTV) in domestic and wild ruminants, to our knowledge, there have never been reports of clinical illness or fatalities attributed to either of these viruses. Two free ranging white-tailed deer (Odocoileus virginianus) in two licensed hunting ranches in Northern Mexico near the Texas border died unexpectedly. Postmortem and microscopic examinations revealed hemorrhagic lesions compatible with viral hemorrhagic disease (Reoviridae: Orbivirus). Tissues from one animal tested positive by RT-PCR for BTV but negative for EHDV. To our knowledge, this is the first time in Mexico where deer dying with hemorrhagic lesions consistent with Bluetongue tested positive for BTV by PCR. Key words: hemorrhagic disease, white-tailed deer, Orbivirus, Mexico

Deshidratación osmótica de frutos de papaya hawaiiana (carica papaya l.) en cuatro agentes edulcorantes.

Trozos de papaya hawaiiana (Carica papaya L.) fueron sometidos a un proceso de osmodeshidratación usando cuatro agentes edulcorantes: miel de abejas, miel de caña, crema demiel de abejas y sacarosa en medio acuoso a 79 grados Brix, temperatura de 20 ºC y 23 horas de inmersión. Los resultados estadísticos mostraron que el agente de mayor capacidad deshidratante fue la miel de abejas y el menor la sacarosa. Además, los análisis cinéticos indicaron que la máxima transferencia de masa ocurre en las primeras cuatro horas del proceso y la máxima pérdida de masa del producto que puede ser alcanzada fue de 32 % con un contenido de humedad final en los frutos de papaya osmodeshidratada de 41,3 % b.h

Dental treatment under general anesthesia in mentally disabled patients based on an ambulatory surgery model : a Case-control study

Mentally disabled patients commonly offer little or no cooperation in dental treatments, and general anesthesia may become necessary in such cases. The present study was to identify the most relevant factors in dental treatment under general anesthesia i

On the interaction of the North Andes plate with the Caribbean and South American plates in northwestern South America from GPS geodesy and seismic data

We examine the hypocentral distribution of seismicity and a series of geodetic velocity vectors obtained from Global Positioning System observations between 1994 and 2015 both offshore and mainland northwestern South America (66°W–77°W; 8°N–14°N). Our analysis, that includes a kinematic block modelling, shows that east of the Caribbean–South American–North Andes plates’ triple junction at ∼68°W; 10.7°N, right-lateral easterly oriented shear motion (∼19.6 ± 2.0 mm yr−1) between the Caribbean and South America plates is split along two easterly striking, right-lateral strike-slip subparallel fault zones: the San Sebastián fault that runs off-shore the Venezuelan coast and slips about 17.0±0.5mm yr−1 and the La Victoria fault, located on-shore to the south, which is accumulating strain equivalent to 2.6 ± 0.4 mm yr−1.West of the triple junction, relative right-lateral motion between the Caribbean and South American plates is mostly divided between the Morrocoy and Boconó fault systems that strike northwest and southwest from the triple junction, respectively, and bound the intervening North Andes plate that shows an easterly oriented geodetic slip of 15.0 ± 1.0 mm yr−1 relative to the South American plate. Slip on the Morrocoy fault is right-lateral and transtensional. Motion across the Boconó fault is also right-lateral but instead transpressional, divided between ∼9 and 11 mm yr−1 of right-slip on the Bocon´o fault and 2–5 mm yr−1 of convergence across adjacent and subparallel thrust faults. Farther west of the triple junction, ∼800 km away in northern Colombia, the Caribbean plate subducts to the southeast beneath the North Andes plate at a geodetically estimated rate of ∼5–7 mm yr−1

RF Acquisition System Based on μTCA for Testing of High-Gradient Acceleration Cavities

The radio frequency (RF) laboratory hosted in the Corpuscular Physics Institute (IFIC) of the University of Valencia is designed to house a high-power and high-repetition-rate facility to test normal conduction RF accelerator cavities in the S-Band (2.9985 GHz) in order to perform R&D activities related to particle accelerator cavities. The system, which manages the entire process of RF signal generation, data acquisition and closed-loop control of the laboratory, is currently based on a modular and compact PXI platform system. This contribution details the development of a platform with similar features, but which is based on open architecture standards at both the hardware and software level. For this purpose, a complete system based on the μTCA platform has been developed. This new system must be able to work with accelerator cavities at other operating frequencies, such as 750 MHz, as well as to explore different options at firmware and software levels based on open-source codes

Human pregnane X receptor is expressed in breast carcinomas, potential heterodimers formation between hPXR and RXR-alpha.

The human pregnane X receptor (hPXR) is an orphan nuclear receptor that induces transcription of response elements present in steroid-inducible cytochrome P 450 gene promoters. This activation requires the participation of retinoid X receptors (RXRs), needed partners of hPXR to form heterodimers. We have investigated the expression of hPXR and RXRs in normal, premalignant, and malignant breast tissues, in order to det. whether their expression profile in localized infiltrative breast cancer is assocd. with an increased risk of recurrent disease. Methods: Breast samples from 99 patients including benign breast diseases, in situ and infiltrative carcinomas were processed for immunohistochem. and Western-blot anal. Results: Cancer cells from patients that developed recurrent disease showed a high cytoplasmic location of both hPXR isoforms. Only the infiltrative carcinomas that relapsed before 48 mo showed nuclear location of hPXR isoform 2. This location was assocd. with the nuclear immunoexpression of RXR-alpha. Conclusion: Breast cancer cells can express both variants 1 and 2 of hPXR. Infiltrative carcinomas that recurred showed a nuclear location of both hPXR and RXR-alpha; therefore, the overexpression and the subcellular location changes of hPXR could be considered as a potential new prognostic indicator

Auditoria forense como servicio especializado para la prevención del fraude

The objective of this research proposal is to study forensic auditing as a specialized service for fraud prevention, based on the authors Cano, D., Lugo, D., Cano, M., & Cano, J. (2009), ACFE (2022), Mendoza (2009), among others. For the design of this proposal, documents such as scientific research articles, official statistics, degree works were consulted, which refer to the study variable framed in the forensic audit and its effect on the detection of fraud. Methodologically, it is a descriptive study under a qualitative approach, consisting of a field design and a documentary study. The expected results will allow us to answer the problem question raised on how the forensic audit as a specialized service helps to prevent fraud? Likewise, it is expected to generate relevant conclusions in response to the specific objectives formulated in the design of this research proposal. with the intention of responding to the general objective and proposing recommendations based on the study, if necessary.El objetivo de esta propuesta de investigación es estudiar la auditoria forense como servicio especializado para la prevención del fraude, basado en los autores Cano, D., Lugo, D., Cano, M., & Cano, J. (2009), ACFE (2022), Mendoza (2009), entre otros. Para el    diseño de esta propuesta se consultó documentos como artículos de investigación científica, estadísticas oficiales, trabajos de grado, que referencian la variable de estudio enmarcadas en la auditoria forense y su efecto en la detección de fraudes. Metodológicamente, se trata de un estudio descriptivo bajo un enfoque cualitativo, constituido por un diseño de campo y estudio documental. Los resultados esperados permitirán dar respuesta a la pregunta problema planteada sobre ¿cómo la auditoria forense como servicio especializado ayuda a la prevención del fraude?, así mismo se espera generar conclusiones pertinentes en atención a los objetivos específicos formulados en el diseño de esta propuesta investigativa, con intenciones de responder al objetivo general y proponer recomendaciones a partir del estudio de ser necesario

El aprendizaje a saltos mediante el uso de equipos de radio definida por software para la docencia en el grado en ingeniería electrónica de telecomunicación

La enseñanza de las comunicaciones conlleva situaciones de aprendizaje diferentes según el contexto en el que se desarrollen. El contexto actual conlleva el cambio de modelo del habitual totalmente presencial en la docencia a situaciones híbridas en línea y presenciales. Esta situación plantea retos como el desarrollar el aspecto práctico del aprendizaje de los contenidos de comunicaciones y en segundo lugar armonizar las limitaciones de presencialidad con aplicaciones prácticas de las mismas. Este proyecto de innovación docente pretende desarrollar metodologías de trabajo cercanos a los entornos profesionales en el ámbito de la Ingeniería mediante el uso de dispositivos específicos como son los basados en radio definidas por software. Estos dispositivos conjuntamente con una metodología de aprendizaje basada en proyectos (ABP) y una enseñanza que combine la docencia asíncrona y presencial, a saltos, permitirá crear un entorno muy favorable para la adquisición de competencias y habilidades profesionales de forma práctica. La puesta en marcha de sesiones híbridas para el desarrollo de proyectos de sistemas de telecomunicaciones con docencia asíncrona y trabajo en grupo se ha evaluado en este artículo. El grupo bajo estudio se ha evaluado mediante encuestas de tipo likert y ha mostrado una mejora en todos los aspectos evaluados superiores al 40% frente a la situación inicial sin estas innovaciones.UV-SFPIE PID20-1354055How to design communications systems and its foundations depends on various factors such as the accessibility to learning resources, the academic environment and the audience itself. This article reports how the introduction of learning methodologies based on project based learning (PBL), practical devices and asynchronous working has modified the perception of the students about their learning aptitudes. The combination of traditional and offline sessions, with the PBL methodology to perform practical communications systems demonstrations based on professional SDR devices by group of students is presented and analysed in this article. The quantified outcome of their academic involvement due to this innovation is evaluated. The indicators shows that the overall dimensions of student learning of communications systems were improved compared with previous sessions based on simulated, non-groupal and magistral lectures