Effect of elevated inorganic carbon on the cytosolic homeostasis of NO3- in the marine angiosperm Posidonia oceanica (L.) Delile

The marine angiosperm Posidonia oceanica is a mediterranean endemism of great ecological significance. As other marine plants, P. oceanica has adapted secondarily to the marine environment and develop anew different mechanisms to colonize it. Among others, this plant has developed a plasma membrane system for the direct uptake of bicarbonate. In this work we have developed both NO3- and Cl- selective microelectrodes for the continuous monitoring of the intracellular (cytosolic) NO3- and Cl-. In the light, leaf mesophyll cells show a cytosolic NO3- concentration of 5.7±0.2 mM (n=10), while in the dark cytosolic NO3- raises up to 8.7±1.1 mM; these values are in the range of concentrations quoted for Arabidopsis thaliana (Cookson et al., 2005). The enrichment of natural seawater (NSW) with 3 mM NaHCO3 caused a decrease of the cytosolic NO3- concentration of 1 mM and a decrease of the cytosolic concentration of Cl- of 3.5 mM. The saturation of NSW with 1000 µL CO2 L-1 produced a lower diminution of the cytosolic NO3- (0.3 mM). In the presence of 0.1 mM of the plasma membrane permeable inhibitor of the carbonic anhydrase (EZ) the diminution of cytosolic NO3- caused by the same concentration of CO2 was much lower, 0.1 mM. The addition of inorganic carbon, either HCO3- or CO2, has an effect on the cytosolic mechanisms for anionic homeostasis, one of which is the opening of the slow anion channels. These channels are permeable to NO3- and Cl- and could elicit the efflux of these ions. In P. oceanica, the response in the presence of EZ points out that the inorganic carbon species that cause the NO3-/Cl- efflux is HCO3-. This effect could contribute to plant biomass N dilution observed in elevated CO2. References: Cookson et al. 2005. Plant Physiology 138, 1097–1105.Universidad de Málaga. Campus de Excelencia Internacional Andalucía Tec

Investigation on printed antennas for RFID UHF systems for application in optical devices, CDs, DVDs

[SPA]Los sistemas de RFID han experimentado una expansi on considerable en las ultimas d ecadas debido principalmente a su capacidad para reducir la intervenci on humana en los procesos de identi caci on. Este potencial ha provocado el desarrollo de muchas aplica- ciones de RFID utilizadas en las empresas como la trazabilidad alimentaria y el control de inventario. Uno de los problemas de esta tecnolog a aparece cuando las antenas se sit uan cerca de materiales met alicos o l quidos, reduciendo su alcance y abilidad de lectura. En este proyecto se ha investigado el desarrollo de nuevas antenas RFID UHF para ser utilizadas en dispositivos opticos como CDs y DVDs. Para ello hemos desarrollado una interfaz gr a ca de usuario en MATLAB para reducir el tiempo de desarrollo de las antenas.[ENG]RFID systems have experienced a considerable expansion in the recent years due mainly to their ability to reduce human intervention on identi cation processes. This potential have caused the development of many RFID applications to be used in companies as food traceability and control inventory. One problem of RFID UHF antennas is when they are situated near metallic materials or liquids, reducing their reading range and reliability. In this project we have investigated the development of novel RFID UHF antennas to be used on optical devices as CD's and DVD's. For this purpose we have developed a MATLAB GUI to highly reduce the geometric design time of the antennas.Escuela Técnica Superior de Ingeniería de TelecomunicaciónUniversidad Politécnica de Cartagen

HCO3- enrichment causes cytosolic NO3- efflux in Posidonia oceanica leaf cells

Posidonia oceanica is a seagrass, the only group of vascular plants to colonize the marine environment. Seawater is an extreme yet stable environment, characterized by high salinity, alkaline pH and low availability of essential nutrients, such as nitrate or phosphate. In addition, in aquatic environments the supply of CO2 for the photosynthesis is limited by diffusion and therefore many aquatic plants use HCO3- as the inorganic carbon source for photosynthesis. Previous results have shown that Na+ -dependent transport systems operate on the plasma membrane of P. oceanica mesophyll leaf cells for the high-affinity NO3-, Pi or amino acids uptake. Also, a direct transport of HCO3- driven by H+ has been found in this species that provides inorganic carbon for photosynthesis and could be a significant component of a carbon concentrating mechanism in this species. Interestingly, this HCO3- direct uptake caused the efflux of chloride from the cytosol, probably through S-type anion channels, pointing that other anions could also be removed from the cytosol. This hypothesis could be relevant in the case of NO3-, since the decrease of cytosolic NO3- in response to HCO3- enrichment could limit N-assimilation. Here we analyse the effect of HCO3- increase on NO3- uptake and cytosolic homeostasis in P. oceanica. Enrichment of natural seawater with 3 mM HCO3- evokes the on-going decrease of cytosolic NO3-, from 5.7 ± 0.2 to 4.8 ± 0.7 mM after 40 min of treatment. The incubation of P. oceanica leaf pieces in 3 mM HCO3- NSW causes an initial increase of NO3- concentration in the medium. Maximum efflux (21 nmol NO3- gFM-1 min-1) occurs within the first minute of incubation. Then, external NO3- is depleted from the medium at lower net uptake rate than the value observed in non HCO3- -enriched natural seawater. These results fit the hypothesis that HCO3- enrichment causes the nitrogen loose and could impair nitrogen assimilation promoting N biomass impoverishment.Universidad de Málaga. Campus de Excelencia Internacional Andalucía Tech. Spanish MINECO, projects BFU2017-85117-R and BIO2016-81957-RED

Na+-dependent NO3- uptake in leaf cells of the seagrass Posidonia oceanica (L.) Delile

Posidonia oceanica (L.) Delile is an endemic Mediterranean seagrass of recognized ecological significance and, as other seagrasses, this species has secondarily adapted to live in the marine environment. In this alkaline medium with a high Na+ concentration (0.5 M), the high inwardly directed electrochemical potential gradient for sodium is used in the seagrass Zostera marina to energize the uptake of nitrate1 and phosphate that usually occur at concentration below 10 µM. Here we summarize several evidences for the operation of a sodium-dependent high-affinity nitrate transport system at the plasma membrane of the mesophyll leaf cells of P. oceanica. Leaf cells of P. oceanica possess a H+-ATPase as a primary pump, exhibit a plasma membrane potential (Em) of -174 ± 10 mV and show reduced Na+ permeability. The addition of micromolar nitrate concentrations induces membrane depolarizations that show saturation kinetics. Curve fitting of the values renders a semisaturation constant (Km) of 21.3 ± 6.6 μM and a maximum depolarization (Dmax) of 7 ± 1 mV. In dark conditions, Dmax decreases by fifty percent but no significant effect is observed on the Km value. On the other hand, nitrate induced depolarizations show sodium dependence. The depolarizations induced by 100 µM NO3- in media containing increasing Na+ concentrations (from 0 to 250 mM) show saturation kinetics, rendering a Km value of 16 ± 5 mM Na+. Moreover, the depolarization induced by 100 µM NO3- is accompanied by a simultaneous increase of cytosolic sodium, measured by Na+-sensitive microelectrodes, of 0.4 ± 0.2 mM above the resting cytosolic sodium concentration (17 ± 2 mM). Finally, nitrate uptake rates, measured in depletion experiments, decreases by 50% and 80% in dark conditions and in the absence of Na+, respectively, compared with control conditions (0.5 M Na+ and light). All together, these results strongly suggest that NO3- uptake in P. oceanica leaf cells is mediated by a high-affinity nitrate carrier that uses Na+ as the driving ion. 1 Rubio et al. (2005). J. Exp. Bot, 412: 613-622. Project Funding: CTM 2011-30356. (MEC)Universidad de Málaga. Campus de Excelencia Internacional Andalucía Tech

The profitability of value-added products in dairy farm diversification initiatives

A more open and competitive dairy market has encouraged certain dairy farms to adopt value-adding strategies in order to achieve a higher profitability, which may be important for farms’ survival. This paper investigated the role of some product characteristics in the profitability of value-added products in these farms. For this purpose, we used a unique database of 265 different products commercialized by 49 Spanish dairy farms that offers information on nine attributes of each product. Using hedonic models as a baseline, we examined the influence of these attributes on the margin per liter (ML) of the products. The results of the regression indicated that cheese and yogurt generated 0.688 and 1.518 € more of margin per liter than liquid milk. Similarly, we found a set of attributes that have a positive influence on ML, including possession of a certificate of protected designation of origin (PDO), the milk-type composition (proportion of sheep milk), a longer expiration period, and involvement in direct marketing strategies (DMS). However, other recognized attributes such as organic labeling, maturation period, size of the sales unit and returnable packaging did not have a significant influence on ML. Our findings also showed that firms producing more elaborated products as cheese and yogurt need a lower percentage of their production to cover the fixed costs associated to transformation and commercialization. Overall, our results revealed that the elaboration of value-added dairy products improves the profitability of dairy farms

Wastewater Treatment by Advanced Oxidation Process and Their Worldwide Research Trends

Background: Water is a scarce resource and is considered a fundamental pillar of sustainable development. The modern development of society requires more and more drinking water. For this cleaner wastewater, treatments are key factors. Among those that exist, advanced oxidation processes are being researched as one of the sustainable solutions. The main objective of this manuscript is to show the scientific advances in this field. Methods: In this paper, a systematic analysis of all the existing scientific works was carried out to verify the evolution of this line of research. Results: It was observed that the three main countries researching this field are China, Spain, and the USA. Regarding the scientific collaboration between countries, three clusters were detected—one of Spain, one of China and the USA, and one of Italy and France. The publications are grouped around three types of water: industrial, urban, and drinking. Regarding the research, 15 clusters identified from the keywords analyzed the advanced oxidation process (alone or combined with biological oxidation) with the type of wastewater and the target pollutant, removal of which is intended. Finally, the most important scientific communities or clusters detected in terms of the number of published articles were those related to the elimination of pollutants of biological origin, such as bacteria, and of industrial nature, such as pesticides or pharmaceutical products

Introducción

Aquest monogràfic d'Anàlisi presenta avanços i novetats en l'ampli camp de l'alfabetització mediàtica dels joves. Assenyala les qüestions que semblen decisives en aquests moments i ho fa des d'una perspectiva internacional en la qual es combinen una mirada comparativa, en alguns casos, amb l'estudi local –especialment en qüestions que afecta a Europa i Espanya—. És una mostra de l'interès de la investigació en aquest camp i, al mateix temps, de la gradual integració i consolidació de metodologies i punts de vista compartits que s'està experimentant en aquest sector en els últims temps

Association between Eruption Sequence of Posterior Teeth, Dental Crowding, Arch Dimensions, Incisor Inclination, and Skeletal Growth Pattern

Background: We conducted research to investigate the effects of the eruption sequence of posterior teeth, arch dimensions, and incisor inclination on dental crowding. Material and Methods: A cross-sectional analytic study was performed on 100 patients (54 boys and 46 girls; mean ages: 11.69 and 11.16 years, respectively). Seq1 (canine-3-/second premolar-5-) or Seq2 (5/3) eruption sequences were recorded in maxilla, and Seq3 (canine-3-/first premolar-4-) or Seq4 (4/3) eruption sequences in mandible; tooth size, available space, tooth size-arch length discrepancy (TS-ALD), arch lengths, incisor inclination and distance, and skeletal relationship were noted. Results: The most common eruption sequences in the maxilla and mandible were Seq1 (50.6%), and Seq3 (52.1%), respectively. In the maxilla, posterior tooth sizes were larger in crowded cases. In the mandible, anterior and posterior tooth sizes were larger in crowded patients. No relationship between incisor variables and the maxillo-mandibular relationship and dental crowding was found. A negative correlation between inferior TS-ALD and the mandibular plane was found. Conclusions: Seq1 and Seq 2 in the maxilla and Seq 3 and Seq 4 in the mandible were equally prevalent. An eruption sequence of 3–5 in the maxilla and 3–4 in the mandible is more likely to cause crowding.BIOCRAN (Craniofacial Biology, Orthodontics and Dentofacial Orthopedics) Research GroupDepartment of Clinical Spaecialties (DECO), University Complutense of Madri

Positioning Control System for a Large Range 2D Platform with Submicrometre Accuracy for Metrological and Manufacturing Applications

The importance of nanotechnology in the world of Science and Technology has rapidly increased over recent decades, demanding positioning systems capable of providing accurate positioning in large working ranges. In this line of research, a nanopositioning platform, the NanoPla, has been developed at the University of Zaragoza. The NanoPla has a large working range of 50 mm × 50 mm and submicrometre accuracy. The NanoPla actuators are four Halbach linear motors and it implements planar motion. In addition, a 2D plane mirror laser interferometer system works as positioning sensor. One of the targets of the NanoPla is to implement commercial devices when possible. Therefore, a commercial control hardware designed for generic three phase motors has been selected to control and drive the Halbach linear motors.This thesis develops 2D positioning control strategy for large range accurate positioning systems and implements it in the NanoPla. The developed control system coordinates the performance of the four Halbach linear motors and integrates the 2D laser system positioning feedback. In order to improve the positioning accuracy, a self calibration procedure for the characterisation of the geometrical errors of the 2D laser system is proposed. The contributors to the final NanoPla positioning errors are analysed and the final positioning uncertainty (k=2) of the 2D control system is calculated to be ±0.5 µm. The resultant uncertainty is much lower than the NanoPla required positioning accuracy, broadening its applicability scope.<br /

Conflicto y convivencia: profesores y alumnos en el proceso de enseñanza en un aula de secundaria

Esta investigación analiza las relaciones complejas entre un profesor de educación secundaria y sus alumnos en la clase de Ciencias sociales. Se examinan las posibilidades de una práctica docente más reflexiva como estrategia de superación del enfrentamiento entre la cultura de los estudiantes y la cultura escolar, representada por el profesor. A través de la experimentación de un proyecto curricular sobre el conflicto y la convivencia y utilizando una metodología cualitativa, se obtuvieron evidencias en relación con el progreso del desarrollo profesional del docente en tres dimensiones: la concepción de los contenidos escolares, la utilización de una metodología didáctica activa y la reformulación de la identidad profesional en función de un nuevo tipo de relaciones en el aula.This research analyzes the complex relations between a secondary school teacher and his students in a social science class. An examination is made of a more reflective teaching practice as a strategy to overcome confrontation between student culture and school culture, which is represented by the teacher. By experimenting with a curriculum project on conflict and interaction and using qualitative methodology, evidence was obtained in relation to the progress of teachers’ professional development in three dimensions: the conception of scholastic content, the use of active teaching methodology, and the reformulation of professional identity as a function of a new type of relationships in the classroo