Molecular probe technique for determining local thermal transitions: The glass transition at Silica/PMMA nanocomposite interfaces

Local glass transition temperatures (Tg) have been measured in the interfaces of solution blended silica/poly(methyl methacrylate) (PMMA) nanocomposites using florescence spectroscopy and compared with Tg measured by differential scanning calorimetry (DSC). It was found that the two types of measurements yielded significantly different information. Combinations of silanes and poly(propylene glycol)-based molecular spacers bound to fluorophores were covalently linked to the surface of the nanoparticles, allowing for variation of the fluorophore response with respect to the distance from the nanofiller surface. Increases in the bulk Tg from the neat PMMA value were found upon the addition of nanofillers, but were independent of the nanofiller concentration when the filler concentration was above 2% by weight. Furthermore, as the size of the grafted molecular spacer was increased, Tg values were found to decrease and approach Tg of the neat PMMA. Owing to variable conformations of the spacers, an effective distribution of fluorophore-silica distances exists, which influences the fluorophores' response to the transition.This research was supported by the National Science Foundation under Grant No. 0500324. Authors from UC3M would like to acknowledge CICYT for financial support (MAT 2007-63722)

Dynamic validation of the Planck/LFI thermal model

The Low Frequency Instrument (LFI) is an array of cryogenically cooled radiometers on board the Planck satellite, designed to measure the temperature and polarization anisotropies of the cosmic microwave backgrond (CMB) at 30, 44 and 70 GHz. The thermal requirements of the LFI, and in particular the stringent limits to acceptable thermal fluctuations in the 20 K focal plane, are a critical element to achieve the instrument scientific performance. Thermal tests were carried out as part of the on-ground calibration campaign at various stages of instrument integration. In this paper we describe the results and analysis of the tests on the LFI flight model (FM) performed at Thales Laboratories in Milan (Italy) during 2006, with the purpose of experimentally sampling the thermal transfer functions and consequently validating the numerical thermal model describing the dynamic response of the LFI focal plane. This model has been used extensively to assess the ability of LFI to achieve its scientific goals: its validation is therefore extremely important in the context of the Planck mission. Our analysis shows that the measured thermal properties of the instrument show a thermal damping level better than predicted, therefore further reducing the expected systematic effect induced in the LFI maps. We then propose an explanation of the increased damping in terms of non-ideal thermal contacts.Comment: Planck LFI technical papers published by JINST: http://www.iop.org/EJ/journal/-page=extra.proc5/1748-022

Longitudinal Evaluation of the Hypothalamic-Pituitary-Testicular Function in 8 Boys with Adrenal Hypoplasia Congenita (AHC) Due to NR0B1 Mutations

BACKGROUND:Boys carrying mutations in the NR0B1 gene develop adrenal hypoplasia congenita (AHC) and impaired sexual development due to the combination of hypogonadotropic hypogonadism (HH) and primary defects in spermatogenesis. METHODS:We analysed the evolution of hypothalamic-pituitary-testicular function of 8 boys with AHC due to NR0B1 mutations. Our objective was to characterize and monitor the progressive deterioration of this function. RESULTS:The first symptoms appeared in the neonatal period (n = 5) or between 6 months and 8.7 years (n = 3). Basal plasma adrenocorticotrophic hormone (ACTH) concentrations increased in all boys, whilst cortisol levels decreased in one case. The natremia was equal or below 134 mmol/L and kaliemia was over 5 mmol/L. All had increased plasma renin. In 3 of 4 patients diagnosed in the neonatal period and evaluated during the first year, the basal plasma gonadotropins concentrations, and their response to gonadotropin releasing hormone (GnRH) test (n = 2), and those of testosterone were normal. The plasma inhibin B levels were normal in the first year of life. With the exception of two cases these concentrations decreased to below the normal for age. Anti-Müllerian hormone concentrations were normal for age in all except one case, which had low concentrations before the initiation of testosterone treatment. In 3 of the 8 cases the gene was deleted and the remaining 5 cases carried frameshift mutations that are predicted to introduce a downstream nonsense mutation resulting in a truncated protein. CONCLUSIONS:The decreases in testosterone and inhibin B levels indicated a progressive loss of testicular function in boys carrying NR0B1 mutations. These non-invasive examinations can help to estimate the age of the testicular degradation and cryopreservation of semen may be considered in these cases as investigational procedure with the aim of restoring fertility

Network-Guided Analysis of Genes with Altered Somatic Copy Number and Gene Expression Reveals Pathways Commonly Perturbed in Metastatic Melanoma

Cancer genomes frequently contain somatic copy number alterations (SCNA) that can significantly perturb the expression level of affected genes and thus disrupt pathways controlling normal growth. In melanoma, many studies have focussed on the copy number and gene expression levels of the BRAF, PTEN and MITF genes, but little has been done to identify new genes using these parameters at the genome-wide scale. Using karyotyping, SNP and CGH arrays, and RNA-seq, we have identified SCNA affecting gene expression (‘SCNA-genes’) in seven human metastatic melanoma cell lines. We showed that the combination of these techniques is useful to identify candidate genes potentially involved in tumorigenesis. Since few of these alterations were recurrent across our samples, we used a protein network-guided approach to determine whether any pathways were enriched in SCNA-genes in one or more samples. From this unbiased genome-wide analysis, we identified 28 significantly enriched pathway modules. Comparison with two large, independent melanoma SCNA datasets showed less than 10% overlap at the individual gene level, but network-guided analysis revealed 66% shared pathways, including all but three of the pathways identified in our data. Frequently altered pathways included WNT, cadherin signalling, angiogenesis and melanogenesis. Additionally, our results emphasize the potential of the EPHA3 and FRS2 gene products, involved in angiogenesis and migration, as possible therapeutic targets in melanoma. Our study demonstrates the utility of network-guided approaches, for both large and small datasets, to identify pathways recurrently perturbed in cancer

Extensive multiband study of the X-ray rich GRB 050408: A likely off-axis event with an intense energy injection

Aims. Understand the shape and implications of the multiband ligth curve of GRB 050408, an X-ray rich (XRR) burst. Methods. We present a multiband optical light curve, covering the time from the onset of the γ-ray event to several months after, when we only detect the host galaxy. Together with X-ray, millimetre and radio observations we compile what, to our knowledge, is the most complete multiband coverage of an XRR burst afterglow to date. Results. The optical and X-ray light curve is characterised by an early flattening and an intense bump peaking around 6 days after the burst onset. We explain the former by an off-axis viewed jet, in agreement with the predictions made for XRR by some models, and the latter with an energy injection equivalent in intensity to the initial shock. The analysis of the spectral flux distribution reveals an extinction compatible with a low chemical enrichment surrounding the burst. Together with the detection of an underlying starburst host galaxy we can strengthen the link between XRR and classical long-duration bursts. © ESO 2007