Intestinal Microbial Ecology of Broilers Vaccinated and Challenged With Mixed Eimeria Species, and Supplemented with Essential Oil Blends

Intestinal microbiota is an important component in the development of defense mechanisms in the gut mucosa. This project determined the dynamics of intestinal microbial communities (MC) of broilers vaccinated at first day of age with live oocysts of Eimeria species and fed diets supplemented with 2 specific essential oil (EO) blends, Crina Poultry (CP) and Crina Alternate (CA). Five treatments were analyzed: 1) unmedicated-uninfected (UU) control; 2) unmedicated-infected (UI) control; 3) vaccinated with Advent cocci-vaccine and without feed additive (COV) supplements; 4) vaccinated with Advent and supplemented with CP; and 5) vaccinated with Advent and supplemented with CA. The EO blends were added at 100 ppm to the same basal diets. Chicks were gavage-infected at 19 d of age with Eimeria acervulina, Eimeria maxima, and Eimeria tenella. Duodenal, ileal, and cecal samples were taken from 12 birds per treatment just before the infection and 7 d after the challenge, pooled in 6 samples, and frozen. Denaturing gradient gel electrophoresis was used to examine PCR-amplified fragments of the bacterial 16S ribosomal DNA variable region. Results are presented as percentages of similarity coefficients (SC). Dendrograms of amplicon patterns indicated MC differences due to intestinal location, feed additives, and cocci infection. The EO blends CP and CA did affect MC in all gut sections. The cocci-infection caused drastic MC population shifts in duodenal, ileal, and cecal sections (36.7, 55.4, and 36.2% SC, respectively). The CP-supplemented birds had higher SC between pre- and postchallenge MC in duodenal and ileal (73.3, 81.8%) than COV (66.4, 66.5%). However, COV broilers had the smallest changes in cecal MC after infection (79.5% SC). We concluded that cocci-vaccination causes small changes in intestinal MC, but challenge causes drastic shifts. The EO blend supplementation modulates MC in cocci-vaccinated broilers, avoiding drastic shifts after a mixed coccidia infection. Correlations between MC dynamics and host responses are discussed

Formación inicial de profesores de educación media de ciencia mediante la teoría de enseñanza y aprendizaje basada en modelos mentales

Este estudio describe cómo se aplica la Teoría de los Modelos Mentales en la formación inicial del profesor de Ciencias de enseñanza media en la Facultad de Educación de la Universidad de Concepción. Esta nueva modalidad de formación, se introdujo a partir del año 2004, en las asignaturas de Didáctica III y IV y en el desarrollo de la tesis de pregrado. Los sesenta estudiantes participantes de la experiencia generaron más de treinta planificaciones de unidades sobre diferentes temas científicos, en las que se utiliza la Teoría de los Modelos Mentales, y que han aplicado a alumnos de enseñanza media. Los dos estudios pilotos que midieron la efectividad de estas planificaciones, indican que el uso de esta perspectiva genera auspiciosos resultados en el aprendizaje de los estudiantes de enseñanza media y en la organización del quehacer en el aula del futuro docente

Characterization of Ascaris from Ecuador and Zanzibar

To shed light on the epidemiology of ascariasis in Ecuador and Zanzibar, 177 adult worms retrieved by chemo-expulsion from either people or pigs were collected, measured and subjected to polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) analysis of the ribosomal internal transcribed spacer (ITS) region. Upon double digestion with RsaI and HaeIII, PCR-RFLP analysis revealed the presence of A. lumbricoides in people and A. suum in pigs in Ecuador. In contrast, while there are no pigs on Zanzibar, of the 56 worms obtained from people, one was genotyped as A. suum. No additional genetic variation was detected upon further PCR-RFLP analysis with several other restriction enzymes. Upon measurement, worm mass and length differed by location and by species, A. suum being lighter and longer. While there is no evidence to suggest zoonotic transmission in Ecuador, an enduring historical signature of previous zoonotic transmission remains on Zanzibar

Assessing equity in transport accessibility to work and study: The Bogotá region

This research was aimed at exploring levels of equity in accessibility to employment and education in the city-region of Bogotá, Colombia's capital city. Building on consolidated methodologies for the assessment of potential accessibility, we estimate accessibility indicators at the zone level, evaluate how potential accessibility varies among income groups, and present evidence related to transport mode, in order to analyze social and spatial inequalities produced by the distribution of accessibility to employment and education activities. The research incorporates a method to evaluate how accessibility varies among zones according to average income and mode of transport in order to produce evidence-based arguments that can inform transport policy in the city-region of Bogotá, and other similar contexts in the Global South. Our results show strong distributional effects of the socio-spatial and economic structure of the city-region, its transport infrastructure and services, and the effect of current transport and land-use policies for citizens of different income groups. The tools and empirical evidence in this research seek to contribute to informed policy development in Latin America and other developing contexts, and feeding current debates on the role of accessibility in addressing social and spatial inequalities stemming from urban mobility

The Catalytic Asymmetric Intermolecular Prins Reaction

Despite their significant potential, catalytic asymmetric reactions of olefins with formaldehyde are rare and metal-free approaches have not been previously disclosed. Here we describe an enantioselective intermolecular Prins reaction of styrenes and paraformaldehyde to form 1,3-dioxanes, using confined imino-imidodiphosphate (iIDP) Brønsted acid catalysts. Isotope labeling experiments and computations suggest a concerted, highly asynchronous addition of an acid-activated formaldehyde oligomer to the olefin. The enantioenriched 1,3-dioxanes can be transformed into the corresponding optically active 1,3-diols, which are valuable synthetic building blocks

A web-based application to simulate alternatives for sustainable forest management: SIMANFOR

Producción CientíficaGrowth and yield models at different scales are useful tools for forest stakeholders. Adequate simulation of forest stand conditions after different silvicultural scenarios allows stakeholders to adopt appropriate actions to maintain forest integrity while forest products and services are obtained to benefit society as a whole. SIMANFOR is a platform to simulate sustainable forest management alternatives, integrating different modules to manage forest inventories, simulate and project stand conditions and maintain systems security and integrity. SIMANFOR output is compatible with an Office environment (Microsoft or Open), allowing users to exchange data and files between SIMANFOR and their own software. New developments are being planned under a web 2.0 environment to take advantage of user input to improve SIMANFOR in the future

Mozambique: Urban Mobility, Accessibility & COVID-19

What policies have been put in place to limit the spread of COVID-19 in relation to mobility and accessibility

Life cycle assessment of biowaste and green waste composting systems : A review of applications and implementation challenges

Altres ajuts: acords transformatius de la UABComposting is one of the most widely applied methods for recycling organic waste. This process has been proposed as one option that facilitates the reincorporation of materials into the production cycle. However, composting also generates environmental impacts. Life Cycle Assessment (LCA) is the most common approach to evaluate the environmental impacts of a process at different system stages. Nevertheless, applying LCA in composting facilities is challenging due to the extensive information required, the lack of standardization on the initial assumptions, the definition of system boundaries, and the high diversity of existing composting technologies. This paper systematically reviews LCA studies in biowaste and/or green waste composting. The study highlights the challenges that should be met in order to improving the application of LCA to evaluate the environmental impacts of this type or waste treatment strategy. The review protocol used identified 456 papers published between 2010 and 2022. After the screening, 56 papers were selected, read, and thoroughly analyzed. The results show that: i) about 68% of the studies aimed to compare composting with other solid waste management options; ii) there was a wide diversity among the impact categories considered, which predominantly included climate change and ozone depletion; iii) there was no consensus on the functional unit or the system boundaries; iv) the main gaseous emissions studied were ammonia, methane, and nitrogen oxide, which were generally determined by emission factors; v) the avoided environmental impacts associated with the end-product quality and its application as an organic amendment or soil improver were ignored. This work demonstrates the complexity of conducting credible and valid composting LCA studies and proposes seven recommendations for improving the application of this assessment methodology to analyze this waste management alternative

Disrupting the wall accumulation of human sperm cells by artificial corrugation

Many self-propelled microorganisms are attracted to surfaces. This makes their dynamics in restricted geometries very different from that observed in the bulk. Swimming along walls is beneficial for directing and sorting cells, but may be detrimental if homogeneous populations are desired, such as in counting microchambers. In this work, we characterize the motion of human sperm cells $60 \mu m$ long, strongly confined to $25 \mu m$ shallow chambers. We investigate the nature of the cell trajectories between the confining surfaces and their accumulation near the borders. Observed cell trajectories are composed of a succession of quasi-circular and quasi-linear segments. This suggests that the cells follow a path of intermittent trappings near the top and bottom surfaces separated by stretches of quasi-free motion in between the two surfaces, as confirmed by depth resolved confocal microscopy studies. We show that the introduction of artificial petal-shaped corrugation in the lateral boundaries removes the tendency of cells to accumulate near the borders, an effect which we hypothesize may be valuable for microfluidic applications in biomedicine.Comment: 9 pages, latex. In accepted version on April 14, v2: abstract modified, information added to Sec. II.A and experiments added to Sec. III.A and Fig.3. Sec. III.C was deleted. Requested references adde

Enzymes as Feed Additive to Aid in Responses Against Eimeria Species in Coccidia-Vaccinated Broilers Fed Corn-Soybean Meal Diets with Different Protein Levels

This research aimed to evaluate the effects of adding a combination of exogenous enzymes to starter diets varying in protein content and fed to broilers vaccinated at day of hatch with live oocysts and then challenged with mixed Eimeria spp. Five hundred four 1-d-old male Cobb-500 chickens were distributed in 72 cages. The design consisted of 12 treatments. Three anticoccidial control programs [ionophore (IO), coccidian vaccine (COV), and coccidia-vaccine + enzymes (COV + EC)] were evaluated under 3 CP levels (19, 21, and 23%), and 3 unmedicated-uninfected (UU) negative controls were included for each one of the protein levels. All chickens except those in unmedicated-uninfected negative controls were infected at 17 d of age with a mixed oral inoculum of Eimeria acervulina, Eimeria maxima, and Eimeria tenella. Live performance, lesion scores, oocyst counts, and samples for gut microflora profiles were evaluated 7 d postinfection. Ileal digestibility of amino acids (IDAA) was determined 8 d postinfection. Microbial communities (MC) were analyzed by G + C%, microbial numbers were counted by flow cytometry, and IgA concentrations were measured by ELISA. The lowest CP diets had poorer (P ≤ 0.001) BW gain and feed conversion ratio in the preinfection period. Coccidia-vaccinated broilers had lower performance than the ones fed ionophore diets during pre- and postchallenge periods. Intestinal lesion scores were affected (P ≤ 0.05) by anticoccidial control programs, but responses changed according to gut section. Feed additives or vaccination had no effect (P ≥ 0.05) on IDAA, and diets with 23% CP had the lowest (P ≤ 0.001) IDAA. Coccidial infection had no effect on MC numbers in the ileum but reduced MC numbers in ceca and suppressed ileal IgA production. The COV + EC treatment modulated MC during mixed coccidiosis infection but did not significantly improve chicken performance. Results indicated that feed enzymes may be used to modulate the gut microflora of cocci-vaccinated broiler chickens