A comprehensive empirical power comparison of univariate goodness-of-fit tests for the Laplace distribution

In this paper, we do a comprehensive survey of all univariate goodness-of-fit tests that we could find in the literature for the Laplace distribution, which amounts to a total of 45 different test statistics. After eliminating duplicates and considering parameters that yield the best power for each test, we obtain a total of 38 different test statistics. An empirical power comparison study of unmatched size is then conducted using Monte Carlo simulations, with 400 alternatives spanning over 20 families of distributions, for various sample sizes and confidence levels. A discussion of the results follows, where the best tests are selected for different classes of alternatives. A similar study was conducted for the normal distribution in Rom\~ao et al. (2010), although on a smaller scale. Our work improves significantly on Puig & Stephens (2000), which was previously the best-known reference of this kind for the Laplace distribution. All test statistics and alternatives considered here are integrated within the PoweR package for the R software.Comment: 37 pages, 1 figure, 20 table

Mechanisms of active laryngeal closure during non-invasive intermittent positive pressure ventilation in non-sedated lambs

The present study stems from our recent demonstration (Moreau-Bussiere F, Samson N, St-Hilaire M, Reix P, Lafond JR, Nsegbe E, Praud JP. J Appl Physiol 102: 2149-2157, 2007) that a progressive increase in nasal intermittent positive pressure ventilation (nIPPV) leads to active glottal closure in nonsedated, newborn lambs. The aim of the study was to determine whether the mechanisms involved in this glottal narrowing during nIPPV originate from upper airway receptors and/or from bronchopulmonary receptors. Two groups of newborn lambs were chronically instrumented for polysomnographic recording: the first group of five lambs underwent a two-step bilateral thoracic vagotomy using video-assisted thoracoscopic surgery (bilateral vagotomy group), while the second group, composed of six lambs, underwent chronic laryngotracheal separation (isolated upper airway group). A few days later, polysomnographic recordings were performed to assess glottal muscle electromyography during step increases in nIPPV (volume control mode). Results show that active glottal narrowing does not develop when nIPPV is applied on the upper airways only, and that this narrowing is prevented by bilateral vagotomy when nIPPV is applied on intact airways. In conclusion, active glottal narrowing in response to increasing nIPPV originates from bronchopulmonary receptors

Origins of the inhibiting effects of nasal CPAP on non-nutritive swallowing in newborn lambs

The present study was aimed at investigating the principal reflex mechanism by which non-nutritive swallowing (NNS) is inhibited by application of a nasal CPAP 6 cm H2O during quiet sleep (QS) in newborn lambs. Eighteen full-term lambs were chronically instrumented and evenly distributed into 3 separate groups to sort out whether reflex activity originates from the upper or lower airway receptors. Six lambs were tracheotomized, six other lambs underwent a 2-step bilateral intrathoracic vagotomy while the remaining 6 lambs underwent chronic laryngo-tracheal separation (isolated upper airway group). Forty-eight hours after surgery, each nonsedated lamb underwent polysomnographic recordings on 3 consecutive days. States of alertness, NNS and respiratory movements were recorded. Results demonstrate that a CPAP of 6 cm H2O inhibited NNS during QS while administered directly on the lower airways. This NNS inhibition was prevented by eliminating the vagal afferent messages originating from the bronchopulmonary receptors. However, application of CPAP on the upper airways only also inhibited NNS during QS. Finally, the application of a CPAP 6 cm H2O had no systematic effect on NNS-breathing coordination. In conclusion, our results suggest that the inhibiting effect of nasal CPAP 6 cm H2O on NNS is mainly mediated by bronchopulmonary receptors with some participation however of upper airway receptors. Our demonstration that spontaneous, non-nutritive swallowing can be modulated by bronchopulmonary receptor activity further illustrates the physiological interactions between upper and lower airways

Goodness-of-fit tests for Laplace, Gaussian and exponential power distributions based on λ -th power skewness and kurtosis

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Goodness-of-fit tests for Laplace, Gaussian and exponential power distributions based on <i>λ</i>-th power skewness and kurtosis

Temperature data, like many other measurements in quantitative fields, are usually modelled using a normal distribution. However, some distributions can offer a better fit while avoiding underestimation of tail event probabilities. To this point, we extend Pearson's notions of skewness and kurtosis to build a powerful family of goodness-of-fit tests based on Rao's score for the exponential power distribution EPDλ(μ,σ), including tests for normality and Laplacity when λ is set to 1 or 2. We find the asymptotic distribution of our test statistic, which is the sum of the squares of two Z-scores, under the null and under local alternatives. We also develop an innovative regression strategy to obtain Z-scores that are nearly independent and distributed as standard Gaussians, resulting in a χ22 distribution valid for any sample size (up to very high precision for n≥20). The case λ=1 leads to a powerful test of fit for the Laplace(μ,σ) distribution, whose empirical power is superior to all 39 competitors in the literature, over a wide range of 400 alternatives. Theoretical proofs in this case are particularly challenging and substantial. We applied our tests to three temperature datasets. The new tests are implemented in the R package PoweR.</p

The Quebec rural emergency department project: a cross-sectional study of a potential two-pronged strategy in the knowledge transfer process.

Health services research generates useful knowledge. Promotion of implementation of this knowledge in medical practice is essential. Prior to initiation of a major study on rural emergency departments (EDs), we deployed two knowledge transfer strategies designed to generate interest and engagement from potential knowledge users. The objective of this paper was to review: 1) a combined project launch and media press release strategy, and 2) a pre-study survey designed to survey potential knowledge users' opinions on the proposed study variables.We evaluated the impact of the project launch (presentation at two conferences hosted by key stakeholders) and media press release via a survey of participants/stakeholders and by calculating the number of media interview requests and reports generated. We used a pre-study survey to collect potential key stakeholder' opinions on the study variables.Twenty-one of Quebec's 26 rural EDs participated in the pre-study survey (81% participation rate). The press release about the study generated 51 press articles and 20 media request for interviews, and contributed to public awareness of a major rural research initiative. In the pre-study survey, thirteen participants (46%) mentioned prior knowledge of the research project. Results from the pre-study survey revealed that all of the potential study variables were considered to be relevant for inclusion in the research project. Respondents also proposed additional variables of interest, including factors promoting retention of human resources.The present study demonstrated the potential utility of a two-pronged knowledge transfer strategy, including a combined formal launch and press release, and a pre-study survey designed to ensure that the included variables were of interest to participants and stakeholders

miR-223 exerts translational control of proatherogenic genes in macrophages

Background: A significant burden of atherosclerotic disease is driven by inflammation. Recently, microRNAs (miRNAs) have emerged as important factors driving and protecting from atherosclerosis. miR-223 regulates cholesterol metabolism and inflammation via targeting both cholesterol biosynthesis pathway and NFkB signaling pathways; however, its role in atherosclerosis has not been investigated. We hypothesize that miR-223 globally regulates core inflammatory pathways in macrophages in response to inflammatory and atherogenic stimuli thus limiting the progression of atherosclerosis. Methods and Results: Loss of miR-223 in macrophages decreases Abca1 gene and protein expression as well as cholesterol efflux to apoA1 (Apolipoprotein A1) and enhances proinflammatory gene expression. In contrast, overexpression of miR-223 promotes the efflux of cholesterol and macrophage polarization toward an anti-inflammatory phenotype. These beneficial effects of miR-223 are dependent on its target gene, the transcription factor Sp3. Consistent with the antiatherogenic effects of miR-223 in vitro, mice receiving miR223(-/-) bone marrow exhibit increased plaque size, lipid content, and circulating inflammatory cytokines (ie, IL-1 beta). Deficiency of miR-223 in bone marrow-derived cells also results in an increase in circulating pro-atherogenic cells (total monocytes and neutrophils) compared with control mice. Furthermore, the expression of miR-223 target gene (Sp3) and pro-inflammatory marker (Il-6) are enhanced whereas the expression of Abca1 and anti-inflammatory marker (Retnla) are reduced in aortic arches from mice lacking miR-223 in bone marrow-derived cells. In mice fed a high-cholesterol diet and in humans with unstable carotid atherosclerosis, the expression of miR-223 is increased. To further understand the molecular mechanisms underlying the effect of miR-223 on atherosclerosis in vivo, we characterized global RNA translation profile of macrophages isolated from mice receiving wild-type or miR223(-/-) bone marrow. Using ribosome profiling, we reveal a notable upregulation of inflammatory signaling and lipid metabolism at the translation level but less significant at the transcription level. Analysis of upregulated genes at the translation level reveal an enrichment of miR-223-binding sites, confirming that miR-223 exerts significant changes in target genes in atherogenic macrophages via altering their translation. Conclusions: Our study demonstrates that miR-223 can protect against atherosclerosis by acting as a global regulator of RNA translation of cholesterol efflux and inflammation pathways.No sponso