Factors controlling the community structure of picoplankton in contrasting marine environments

The effect of inorganic nutrients on planktonic assemblages has traditionally relied on concentrations rather than estimates of nutrient supply. We combined a novel dataset of hydrographic properties, turbulent mixing, nutrient concentration, and picoplankton community composition with the aims of (i) quantifying the role of temperature, light, and nitrate fluxes as factors controlling the distribution of autotrophic and heterotrophic picoplankton subgroups, as determined by flow cytometry, and (ii) describing the ecological niches of the various components of the picoplankton community. Data were collected at 97 stations in the Atlantic Ocean, including tropical and subtropical open-ocean waters, the northwestern Mediterranean Sea, and the Galician coastal upwelling system of the northwest Iberian Peninsula. A generalized additive model (GAM) approach was used to predict depth-integrated biomass of each picoplankton subgroup based on three niche predictors: sea surface temperature, averaged daily surface irradiance, and the transport of nitrate into the euphotic zone, through both diffusion and advection. In addition, niche overlap among different picoplankton subgroups was computed using nonparametric kernel density functions. Temperature and nitrate supply were more relevant than light in predicting the biomass of most picoplankton subgroups, except for Prochlorococcus and low-nucleic-acid (LNA) prokaryotes, for which irradiance also played a significant role. Nitrate supply was the only factor that allowed the distinction among the ecological niches of all autotrophic and heterotrophic picoplankton subgroups. Prochlorococcus and LNA prokaryotes were more abundant in warmer waters ( \u3e 20°C) where the nitrate fluxes were low, whereas Synechococcus and high-nucleic-acid (HNA) prokaryotes prevailed mainly in cooler environments characterized by intermediate or high levels of nitrate supply. Finally, the niche of picoeukaryotes was defined by low temperatures and high nitrate supply. These results support the key role of nitrate supply, as it not only promotes the growth of large phytoplankton, but it also controls the structure of marine picoplankton communities

Factors structuring microbial communities in highly impacted coastal marine sediments (Mar Menor lagoon, SE Spain)

Coastal marine lagoons are environments highly vulnerable to anthropogenic pressures such as agriculture nutrient loading or runoff from metalliferous mining. Sediment microorganisms, which are key components in the biogeochemical cycles, can help attenuate these impacts by accumulating nutrients and pollutants. The Mar Menor, located in the southeast of Spain, is an example of a coastal lagoon strongly altered by anthropic pressures, but the microbial community inhabiting its sediments remains unknown. Here, we describe the sediment prokaryotic communities along a wide range of environmental conditions in the lagoon, revealing that microbial communities were highly heterogeneous among stations, although a core microbiome was detected. The microbiota was dominated by Delta- and Gammaproteobacteria and members of the Bacteroidia class. Additionally, several uncultured groups such as Asgardarchaeota were detected in relatively high proportions. Sediment texture, the presence of Caulerpa or Cymodocea, depth, and geographic location were among the most important factors structuring microbial assemblages. Furthermore, microbial communities in the stations with the highest concentrations of potentially toxic elements (Fe, Pb, As, Zn, and Cd) were less stable than those in the non-contaminated stations. This finding suggests that bacteria colonizing heavily contaminated stations are specialists sensitive to change.The study of the microbial communities has been carried out within the project “Metafluidics” and it was funded by the European Union’s framework program Horizon 2020 (LEIT-BIO-2015-685474 to JA). Samplings were supported by the projects MEMM (financed by the Spanish Institute of Oceanography), 19-ESMARES2-ANG (financed by the Spanish Ministry of Ecological Transition and Demographic Challenge), and BIOFOM (Ref. TEC0004869 financed by the Regional Government of Murcia). M-DB and JB-E were supported by a contract within the Program Personal Técnico de Apoyo funded by the Ministerio de Economía y Competitividad. BA-R was also supported by the ACIF fellow of the Generalitat Valenciana government

Working group on cephalopod fisheries and life history (Wgceph; outputs from 2022 meeting)

Rapports Scientifiques du CIEM. Volume 5, nº 1WGCEPH worked on six Terms of Reference. These involved reporting on the status of stocks; reviewing advances in stock identification, assessment for fisheries management and for the Ma- rine Strategy Framework Directive (MSFD), including some exploratory stock assessments; re- viewing impacts of human activities on cephalopods; developing identification guides and rec- ommendations for fishery data collection; describing the value chain and evaluating market driv- ers; and reviewing advances in research on environmental tolerance of cephalopods. ToR A is supported by an annual data call for fishery and survey data. During 2019–2021, com- pared to 1990–2020, cuttlefish remained the most important cephalopod group in terms of weight landed along the European North Atlantic coast, while loliginid squid overtook octopus as the second most important group. Short-finned squid remained the least important group in land- ings although their relative importance was almost double in 2019–2022 compared to 1992–2020. Total cephalopod landings have been fairly stable since 1992. Cuttlefish landings are towards the low end of the recent range, part of a general downward trend since 2004. Loliginid squid landings in 2019 were close to the maximum seen during the last 20 years but totals for 2020 and 2021 were lower. Annual ommastrephid squid landings are more variable than those of the other two groups and close to the maximum seen during 1992– 2021. Octopod landings have generally declined since 2002 but the amount landed in 2021 was higher than in the previous four years. Under ToR B we illustrate that the combination of genetic analysis and statolith shape analysis is a promising method to provide some stock structure information for L. forbsii. With the sum- mary of cephalopod assessments, we could illustrate that many cephalopod species could al- ready be included into the MSFD. We further provide material from two reviews in preparation, covering stock assessment methods and challenges faced for cephalopod fisheries management. Finally, we summarise trends in abundance indices, noting evidence of recent declines in cuttle- fish and some octopuses of the genus Eledone. Under ToR C, we describe progress on the reviews of (i) anthropogenic impacts on cephalopods and (ii) life history and ecology. In relation to life history, new information on Eledone cirrhosa from Portugal is included. Under ToR D we provide an update on identification guides, discuss best practice in fishery data collection in relation to maturity determination and sampling intensity for fishery monitoring. Among others, we recommend i) to include the sampling of cephalopods in any fishery that (a) targets cephalopods, (b) targets both cephalopods and demersal fishes or (c) takes cephalopods as an important bycatch, ii) Size-distribution sampling, iii) the use of standardized sampling pro- tocols, iv) an increased sampling effort in cephalopod. Work under ToR E on value chains and market drivers, in conjunction with the Cephs & Chefs INTERREG project, has resulted in two papers being submitted. Abstracts of these are in the report. Finally, progress under ToR F on environmental tolerance limits of cephalopods and climate en- velope models is discussed, noting the need to continue this work during the next cycle.info:eu-repo/semantics/publishedVersio

The Holocene stratified screes from Sierra de Albarracín (Iberian Ranges, Spain) and their paleoenvironmental significance

The most important stratified screes of the Iberian Range are found in Sierra de Albarracín. These slope deposits have been traditionally considered, without absolute datings, as having been formed during various Pleistocene cold phases. The aim of this paper is to establish the sedimentological, morphological, chronological, and paleoenvironmental characteristics of these deposits through the study of four profiles recorded in the Calomarde canyon (El Rollo, El Molino, and Royuela) and Toril. The most representative profile is that of El Rollo as it is formed by basal tufa and stratified scree layers separated by paleosoils. Radiocarbon datings obtained from paleosoil samples show that the sequence ranges between the early and middle Holocene. The profiles from El Molino and Royuela, as well as the upper levels of Toril, complete the sequence showing deposits from upper Holocene (Bronze Age and ‘Little Ice Age’). These data show the oscillations during the Holocene between colder phases, represented by the stratified screes, and warmer–wetter phases with soil development and local tufa deposits. This geomorphological and pedological response to the Holocene climatic variability shows its clearest records in the canyons. However, there are almost no Pleistocene accumulations – with the exception of that of Toril (minimum age of <43.5 ka BP). The possibility of relating this succession of Holocene environmental changes to known regional and global climatic stages converts these accumulations into the most important Holocene paleoenvironmental record from the Iberian Ranges and the most complete sequence of Holocene stratified screes from the Mediterranean area.Fil: Peña Monné, José Luis. Universidad de Zaragoza. Facultad de Filosofía y Letras; EspañaFil: Pérez Alberti, Augusto. Universidad de Santiago de Compostela; EspañaFil: Sampietro Vattuone, Maria Marta. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico - Tucumán. Unidad Ejecutora Lillo; Argentina. Universidad Nacional de Tucumán. Facultad de Ciencias Naturales e Instituto Miguel Lillo. Laboratorio de Geoarqueología; ArgentinaFil: Otero, Xose L.. Universidad de Santiago de Compostela; EspañaFil: Sánchez Fabre, Miguel. Universidad de Zaragoza. Facultad de Filosofía y Letras; EspañaFil: Longares Aladrén, Luis A.. Universidad de Zaragoza. Facultad de Filosofía y Letras; Españ

Pyrolysis-gas chromatography/mass spectrometry of soil organic matter extracted from a Brazilian mangrove and Spanish salt marshes

The soil organic matter (SOM) extracted under different vegetation types from a Brazilian mangrove (Pai Matos Island, São Paulo State) and from three Spanish salt marshes (Betanzos Ría and Corrubedo Natural Parks, Galícia, and the Albufera Natural Park, Valencia) was investigated by pyrolysis-gas chromatography/mass spectrometry (Py-GC/MS). The chemical variation was larger in SOM from the Spanish marshes than in the SOM of the Brazilian mangroves, possibly because the marshes included sites with both tidal and nontidal variation, whereas the mangrove forest underwent just tidal variation. Thus, plant-derived organic matter was better preserved under permanently anoxic environments. Moreover, given the low number of studied profiles and sedimentary¿vegetation sequences in both areas, depth trends remain unclear. The chemical data also allow distinction between the contributions of woody and nonwoody vegetation inputs. Soil organic matter decomposition was found to cause: (i) a decrease in lignin contents and a relative increase in aliphatics; (ii) an increase in short-chain aliphatics at the expense of longer ones; (iii) a loss of odd-over-even dominance in alkanes and alkenes; and (iv) an increase in microbial products, including proteins, sterols, short-chain fatty acids, and alkanes. Pyrolysis-gas chromatography/mass spectrometry is a useful tool to study the behavior and composition of SOM in wetland environments such as mangroves and salt marshes. Additional profiles need to be studied for each vegetation type, however, to improve the interpretability of the chemical dat

Los derrubios estratificados holocenos del cañón de Calomarde (Sierra de Albarracín, Teruel) y su reconstrucción paleoambiental

En el cañón de Calomarde, Sierra de Albarracín, se localiza una de las acumulaciones de derrubios estratificados más importante de la Cordillera Ibérica. Estos depósitos de ladera han sido atribuidos tradicionalmente a periodos fríos del Pleistoceno. En el perfil analizado en este trabajo se han diferenciado cuatro etapas de este tipo de acumulaciones de ambiente frío separadas por paleosuelos, en los que ha sido posible efectuar cuatro dataciones 14C. Los resultados sitúan el conjunto en el Holoceno inferior y medio. Estos datos junto con la presencia de tobas calizas en la parte basal del perfil han permitido establecer una secuencia paleoambiental en la que se alternan periodos de fuerte activación de la gelifracción y desarrollo de derrubios estratificados, con fases de estabilidad marcadas por los niveles edáficos y acumulación de tobas calizas en el fondo del valle. Finalmente se establece una relación parcial con eventos climáticos holocenos de carácter regional y global.In the Calomarde canyon, Sierra de Albarracín, one of the most important accumulations of stratified debris in the Iberian Mountain Range is located. These hillside deposits have traditionally been attributed to cold periods of the Pleistocene. In the profile analyzed in this work, four stages of this type of cold environment accumulations separated by paleosols have been distinguished, in which it has been possible to carry out four 14C dates. The results place the whole in the lower and middle Holocene. These data, together with the presence of limestone tuffs in the basal part of the profile, have made it possible to establish a palaeoenvironmental sequence in which periods of strong activation of gelifraction and the development of stratified debris alternate, with stability phases marked by edaphic levels and accumulation. of limestone tuffs at the bottom of the valley. Finally, a partial relationship is established with regional and global Holocene climatic events.Fil: Peña Monné, Jose Luis. Universidad de Zaragoza. Facultad de Filosofía y Letras; EspañaFil: Pérez Alberti, Augusto. Universidad de Santiago de Compostela; EspañaFil: Sampietro Vattuone, Maria Marta. Universidad Nacional de Tucumán. Facultad de Ciencias Naturales e Instituto Miguel Lillo. Laboratorio de Geoarqueología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán; ArgentinaFil: Otero, Xose L.. Universidad de Santiago de Compostela; EspañaFil: Sánchez Fabre, Miguel Angel. Universidad de Zaragoza. Facultad de Filosofía y Letras; EspañaFil: Longares Aladrén, Luis Alberto. Universidad de Zaragoza. Facultad de Filosofía y Letras; Españ

Inputs of seabird guano alter microbial growth, community composition and the phytoplankton–bacterial interactions in a coastal system

Seabird guano enters coastal waters providing bioavailable substrates for microbial plankton, but their role in marine ecosystem functioning remains poorly understood. Two concentrations of the water soluble fraction (WSF) of gull guano were added to different natural microbial communities collected in surface waters from the Ría de Vigo (NW Spain) in spring, summer, and winter. Samples were incubated with or without antibiotics (to block bacterial activity) to test whether gull guano stimulated phytoplankton and bacterial growth, caused changes in taxonomic composition, and altered phytoplankton–bacteria interactions. Alteromonadales, Sphingobacteriales, Verrucomicrobia and diatoms were generally stimulated by guano. Chlorophyll a (Chl a) concentration and bacterial abundance significantly increased after additions independently of the initial ambient nutrient concentrations. Our study demonstrates, for the first time, that the addition of guano altered the phytoplankton–bacteria interaction index from neutral (i.e. phytoplankton growth was not affected by bacterial activity) to positive (i.e. phytoplankton growth was stimulated by bacterial activity) in the low-nutrient environment occurring in spring. In contrast, when environmental nutrient concentrations were high, the interaction index changed from positive to neutral after guano additions, suggesting the presence of some secondary metabolite in the guano that is needed for phytoplankton growth, which would otherwise be supplied by bacteria.Universidade de Vigo/CISUGAgencia Estatal de Investigación | Ref. CTM2017‐83362‐