ORGÃOS INTERNOS DE CORDEIROS ALIMENTADOS COM DIFERENTES NÍVEIS DE SILAGEM DE CASCA DE MANDIOCA

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CARACTERÍSTICAS DO MÚSCULO LONGISSIMUS DORSI DE CORDEIROS TEXEL ALIMENTADOS COM DIFERENTES NÍVEIS DE SILAGEM DE CASCA DE MANDIOCA

Na região sul do Estado de Santa Catarina há grande produção de polvilho de mandioca (Manihot sculenta Crantz), sendo que um dos subprodutos da industrialização deste é a casca de mandioca, a qual se conservada sob a forma de silagem pode ser utilizada na alimentação de ruminantes, podendo ser boa alternativa de volumoso na alimentação animal. O experimento foi realizado no Instituto Federal Catarinense de Educação, Ciência e Tecnologia – Campus Sombrio/SC, foram utilizadas as instalações de confinamento do Setor de Ovino-caprinocultura desta unidade. Foram utilizados 16 cordeiros com predominância da raça Texel recém desmamados, os quais foram distribuídos em quatro tratamentos, com quatro repetições. Após o confinamento dos cordeiros que perdurou por 61 dias estes foram abatidos. Após o resfriamento das carcaças por 24 horas essas foram serradas ao meio. Na meia carcaça esquerda na altura da 12 e 13ª costelas foi exposto o músculo Longissimus dorsi para determinação da área de olho de lombo (AOL), medida que expressa a musculosidade existente na carcaça, também foi avaliada a espessura de gordura subcutânea (EGS) da carcaça máxima e mínima, comprimento e largura máxima do músculo Longissimus dorsi. A área de olho de lombo foi semelhante (P>0,05) entre os tratamentos, com valores médios de 11,94 cm². As medidas do músculo Longissimus dorsi não foram influenciadas pela substituição da silagem de milho da fração volumosa da dieta por silagem de casca de mandioca na alimentação de cordeiros com predominância de sangue Texel

Blocking the CTLA-4 and PD-1 pathways during pulmonary paracoccidioidomycosis improves immunity, reduces disease severity, and increases the survival of infected mice

Immune checkpoint pathways, i.e., coinhibitory pathways expressed as feedback following immune activation, are crucial for controlling an excessive immune response. Cytotoxic T lymphocyte antigen-4 (CTLA-4) and programmed cell death protein-1 (PD-1) are the central classical checkpoint inhibitory (CPI) molecules used for the control of neoplasms and some infectious diseases, including some fungal infections. As the immunosuppression of severe paracoccidioidomycosis (PCM), a chronic granulomatous fungal disease, was shown to be associated with the expression of coinhibitory molecules, we hypothesized that the inhibition of CTLA-4 and PD-1 could have a beneficial effect on pulmonary PCM. To this end, C57BL/6 mice were infected with Paracoccidioides brasiliensis yeasts and treated with monoclonal antibodies (mAbs) α-CTLA-4, α-PD-1, control IgG, or PBS. We verified that blockade of CTLA-4 and PD-1 reduced the fungal load in the lungs and fungal dissemination to the liver and spleen and decreased the size of pulmonary lesions, resulting in increased survival of mice. Compared with PBS-treated infected mice, significantly increased levels of many pro- and anti-inflammatory cytokines were observed in the lungs of α-CTLA-4-treated mice, but a drastic reduction in the liver was observed following PD-1 blockade. In the lungs of α-CPI and IgG-treated mice, there were no changes in the frequency of inflammatory leukocytes, but a significant reduction in the total number of these cells was observed. Compared with PBS-treated controls, α-CPI- and IgG-treated mice exhibited reduced pulmonary infiltration of several myeloid cell subpopulations and decreased expression of costimulatory molecules. In addition, a decreased number of CD4+ and CD8+ T cells but sustained numbers of Th1, Th2, and Th17 T cells were detected. An expressive reduction in several Treg subpopulations and their maturation and suppressive molecules, in addition to reduced numbers of Treg, TCD4+, and TCD8+ cells expressing costimulatory and coinhibitory molecules of immunity, were also detected. The novel cellular and humoral profiles established in the lungs of α-CTLA-4 and α-PD-1-treated mice but not in control IgG-treated mice were more efficient at controlling fungal growth and dissemination without causing increased tissue pathology due to excessive inflammation. This is the first study demonstrating the efficacy of CPI blockade in the treatment of pulmonary PCM, and further studies combining the use of immunotherapy with antifungal drugs are encouraged

SILAGEM DE CASCA DE MANDIOCA EM SUBSTITUIÇÃO A SILAGEM DA PLANTA INTEIRA DE MILHO NA ALIMENTAÇÃO DE CORDEIROS - BIOMETRIA IN VIVO

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CORTES COMERCIAIS DA CARCAÇA DE CORDEIROS ALIMENTADOS COM DIFERENTES NÍVEIS DE SILAGEM DE CASCA DE MANDIOCA

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Rationale, study design, and analysis plan of the Alveolar Recruitment for ARDS Trial (ART): Study protocol for a randomized controlled trial

Background: Acute respiratory distress syndrome (ARDS) is associated with high in-hospital mortality. Alveolar recruitment followed by ventilation at optimal titrated PEEP may reduce ventilator-induced lung injury and improve oxygenation in patients with ARDS, but the effects on mortality and other clinical outcomes remain unknown. This article reports the rationale, study design, and analysis plan of the Alveolar Recruitment for ARDS Trial (ART). Methods/Design: ART is a pragmatic, multicenter, randomized (concealed), controlled trial, which aims to determine if maximum stepwise alveolar recruitment associated with PEEP titration is able to increase 28-day survival in patients with ARDS compared to conventional treatment (ARDSNet strategy). We will enroll adult patients with ARDS of less than 72 h duration. The intervention group will receive an alveolar recruitment maneuver, with stepwise increases of PEEP achieving 45 cmH(2)O and peak pressure of 60 cmH2O, followed by ventilation with optimal PEEP titrated according to the static compliance of the respiratory system. In the control group, mechanical ventilation will follow a conventional protocol (ARDSNet). In both groups, we will use controlled volume mode with low tidal volumes (4 to 6 mL/kg of predicted body weight) and targeting plateau pressure <= 30 cmH2O. The primary outcome is 28-day survival, and the secondary outcomes are: length of ICU stay; length of hospital stay; pneumothorax requiring chest tube during first 7 days; barotrauma during first 7 days; mechanical ventilation-free days from days 1 to 28; ICU, in-hospital, and 6-month survival. ART is an event-guided trial planned to last until 520 events (deaths within 28 days) are observed. These events allow detection of a hazard ratio of 0.75, with 90% power and two-tailed type I error of 5%. All analysis will follow the intention-to-treat principle. Discussion: If the ART strategy with maximum recruitment and PEEP titration improves 28-day survival, this will represent a notable advance to the care of ARDS patients. Conversely, if the ART strategy is similar or inferior to the current evidence-based strategy (ARDSNet), this should also change current practice as many institutions routinely employ recruitment maneuvers and set PEEP levels according to some titration method.Hospital do Coracao (HCor) as part of the Program 'Hospitais de Excelencia a Servico do SUS (PROADI-SUS)'Brazilian Ministry of Healt

Turboatomizador e repasse com pistola manual na cobertura de pulverização de agrotóxicos em caquizeiro (Diospyros kaki L.f.) Air-jet sprayer and spray gun in the pesticide spray coverage of persimmon (Diospyros kaki L.f.) trees

A cultura do caquizeiro vem crescendo em importância no Brasil e, com o aumento do cultivo, tem havido também incremento dos problemas relacionados a doenças e pragas. A pulverização de agrotóxicos é o método mais utilizado na prevenção e no controle desses problemas e a principal forma de aplicação é a pulverização hidráulica com auxílio de fluxo de ar. O objetivo deste trabalho foi avaliar o efeito da variação da taxa de aplicação e do repasse na parte interna das plantas em pulverização com pistola manual, na cobertura de calda das folhas. A pulverização foi realizada em plantas de caquizeiro com 17 anos, utilizando-se equipamento composto de trator e pulverizador turboatomizador. A aplicação foi realizada com duas velocidades de avanço do equipamento, o que promoveu diferentes taxas de aplicação. Para a realização do repasse, utilizou-se pistola manual acoplada ao mesmo equipamento. Para a avaliação da cobertura, utilizou-se calda preparada com corante fluorescente dissolvido em água. Foram coletadas folhas nas posições externa e interna das plantas. Posteriormente, em sala escura e com iluminação ultravioleta para destacar a área atingida pelo corante fluorescente, as superfícies abaxial e adaxial de cada folha foram fotografadas com câmera digital. Cada imagem gerada foi submetida à análise pelo software SIARCS 3.0, resultando em porcentagem de cobertura. O delineamento experimental utilizado foi de blocos ao acaso em parcelas subsubdivididas com quatro repetições, sendo os tratamentos arranjados em esquema fatorial (taxas alta e baixa de aplicação, com e sem repasse manual, localizações interna e externa da folha na planta). Foi avaliada a cobertura das superfícies abaxial e adaxial das folhas. Houve interação significativa entre tratamentos (taxa de aplicação e repasse manual), posição e superfície das folhas. A maior taxa de aplicação promoveu aumento significativo da cobertura na superfície abaxial das folhas, independentemente da posição destas na planta (interna ou externa). Houve efeito do repasse com pistola na cobertura da superfície abaxial das folhas situadas na posição interna, mas não na externa à planta. Considerando que a maioria dos agentes causadores de danos fitossanitários inicia a ocorrência na superfície abaxial das folhas, pode-se concluir que o repasse manual foi mais eficiente do que o aumento da taxa de aplicação, visando à cobertura nas folhas posicionadas no interior da planta.Persimmon crop is growing in importance in Brazil, and with the increase of the production area there also has been an increase in the problems related to diseases and pests. For the control and prevention of these problems, most often it is used pesticides spray, and one of the main techniques is the spray with the aid of air flow. The objective of this study was to evaluate the effects of changes in the application rate and transfer with a spray gun on the leaves mixture coverage. The spraying was performed using tractor and air flow sprayer. The application was performed with two forward speeds of the equipment, leading to different application rates. The transfer was made with a spray gun attached to the same equipment. For the assessment of the coverage it was used a mixture prepared with fluorescent tracer dissolved in water. Leaves were collected in the inside and outside of the tree. Later, in dark room with ultraviolet lighting to highlight the area affected by the fluorescent tracer, the upper and lower surfaces of each leaf were photographed with a digital camera. Each image was subjected to analysis generated by software SIARCS 3.0, resulting in percentage of coverage. It was used a randomized block design with subdivided parcels with four replications, being the treatments in a 2x2x2 factorial scheme (high or low application rate, with or without transfer, inside or outside the tree). The evaluations were made in the upper and lower leaf surfaces. There was significant interaction between treatments (application rate and spray gun transfer), leaf position and surface. High application rate promoted a significant increase in the coverage of the lower leaves, regardless the site in the plant (inside or outside). The inside leaves had a better coverage with the spray gun transfer. As most of the agents of plant damage begin to occur in the lower surface of leaves, it can conclude that the spray gun transfer was more effective to cover the leaves inside the plant than increasing the application rate

Presentation_1_Blocking the CTLA-4 and PD-1 pathways during pulmonary paracoccidioidomycosis improves immunity, reduces disease severity, and increases the survival of infected mice.pptx

Immune checkpoint pathways, i.e., coinhibitory pathways expressed as feedback following immune activation, are crucial for controlling an excessive immune response. Cytotoxic T lymphocyte antigen-4 (CTLA-4) and programmed cell death protein-1 (PD-1) are the central classical checkpoint inhibitory (CPI) molecules used for the control of neoplasms and some infectious diseases, including some fungal infections. As the immunosuppression of severe paracoccidioidomycosis (PCM), a chronic granulomatous fungal disease, was shown to be associated with the expression of coinhibitory molecules, we hypothesized that the inhibition of CTLA-4 and PD-1 could have a beneficial effect on pulmonary PCM. To this end, C57BL/6 mice were infected with Paracoccidioides brasiliensis yeasts and treated with monoclonal antibodies (mAbs) α-CTLA-4, α-PD-1, control IgG, or PBS. We verified that blockade of CTLA-4 and PD-1 reduced the fungal load in the lungs and fungal dissemination to the liver and spleen and decreased the size of pulmonary lesions, resulting in increased survival of mice. Compared with PBS-treated infected mice, significantly increased levels of many pro- and anti-inflammatory cytokines were observed in the lungs of α-CTLA-4-treated mice, but a drastic reduction in the liver was observed following PD-1 blockade. In the lungs of α-CPI and IgG-treated mice, there were no changes in the frequency of inflammatory leukocytes, but a significant reduction in the total number of these cells was observed. Compared with PBS-treated controls, α-CPI- and IgG-treated mice exhibited reduced pulmonary infiltration of several myeloid cell subpopulations and decreased expression of costimulatory molecules. In addition, a decreased number of CD4+ and CD8+ T cells but sustained numbers of Th1, Th2, and Th17 T cells were detected. An expressive reduction in several Treg subpopulations and their maturation and suppressive molecules, in addition to reduced numbers of Treg, TCD4+, and TCD8+ cells expressing costimulatory and coinhibitory molecules of immunity, were also detected. The novel cellular and humoral profiles established in the lungs of α-CTLA-4 and α-PD-1-treated mice but not in control IgG-treated mice were more efficient at controlling fungal growth and dissemination without causing increased tissue pathology due to excessive inflammation. This is the first study demonstrating the efficacy of CPI blockade in the treatment of pulmonary PCM, and further studies combining the use of immunotherapy with antifungal drugs are encouraged.</p

Rationale, study design, and analysis plan of the Alveolar Recruitment for ARDS Trial (ART): Study protocol for a randomized controlled trial

Background: Acute respiratory distress syndrome (ARDS) is associated with high in-hospital mortality. Alveolar recruitment followed by ventilation at optimal titrated PEEP may reduce ventilator-induced lung injury and improve oxygenation in patients with ARDS, but the effects on mortality and other clinical outcomes remain unknown. This article reports the rationale, study design, and analysis plan of the Alveolar Recruitment for ARDS Trial (ART). Methods/Design: ART is a pragmatic, multicenter, randomized (concealed), controlled trial, which aims to determine if maximum stepwise alveolar recruitment associated with PEEP titration is able to increase 28-day survival in patients with ARDS compared to conventional treatment (ARDSNet strategy). We will enroll adult patients with ARDS of less than 72 h duration. The intervention group will receive an alveolar recruitment maneuver, with stepwise increases of PEEP achieving 45 cmH(2)O and peak pressure of 60 cmH2O, followed by ventilation with optimal PEEP titrated according to the static compliance of the respiratory system. In the control group, mechanical ventilation will follow a conventional protocol (ARDSNet). In both groups, we will use controlled volume mode with low tidal volumes (4 to 6 mL/kg of predicted body weight) and targeting plateau pressure <= 30 cmH2O. The primary outcome is 28-day survival, and the secondary outcomes are: length of ICU stay; length of hospital stay; pneumothorax requiring chest tube during first 7 days; barotrauma during first 7 days; mechanical ventilation-free days from days 1 to 28; ICU, in-hospital, and 6-month survival. ART is an event-guided trial planned to last until 520 events (deaths within 28 days) are observed. These events allow detection of a hazard ratio of 0.75, with 90% power and two-tailed type I error of 5%. All analysis will follow the intention-to-treat principle. Discussion: If the ART strategy with maximum recruitment and PEEP titration improves 28-day survival, this will represent a notable advance to the care of ARDS patients. Conversely, if the ART strategy is similar or inferior to the current evidence-based strategy (ARDSNet), this should also change current practice as many institutions routinely employ recruitment maneuvers and set PEEP levels according to some titration method.13Hospital do Coracao (HCor) as part of the Program 'Hospitais de Excelencia a Servico do SUS (PROADI-SUS)'Brazilian Ministry of Healt