フククウキョウ カ チュウスイ セツジョ ジュツ オ シコウ シタ チュウスイ ケイシツ エン ノ 1レイ

本邦では虫垂憩室炎は比較的稀な疾患であり臨床的に診断が困難である.虫垂炎が疑われる症例の中にも本症例のように虫垂憩室炎が存在する可能性がある.しかしそのような場合でも腹腔鏡下手術が有用と考えられる.慢性に経過し画像上虫垂憩室炎の診断に苦慮したが,治療に鏡視下手術が有用であった一例を経験し文献的な考察を加えて報告した.虫垂の炎症疾患を疑い外科的治療を施行する際には腹腔鏡下手術が望ましいと考えられた.Diverticulum of the vermiform appendix is rarely encounteredin Japan. It is difficult to diagnose diverticulum ofthe appendix preoperatively. A 37-year-old male patientcame to our hospital with presentation of acute abdominalpain in the right lower quadrant. We performed laparoscopicappendectomy under the diagnosis of the suspicion ofacute appendicitis. It showed severe adhesion between thetip of appendix and retroperitoneum with local inflammatoryreaction. Pathological examination revealed severalchronic diverticulitis and acute diverticulitis in the appendix.Laparoscopic surgery is very useful to dissect severeadhesion of appendix. Laparoscopic surgery for diverticulumof the appendix is an effective procedure

Identification and analysis of the resorcinomycin biosynthetic gene cluster

<div><p>Resorcinomycin (1) is composed of a nonproteinogenic amino acid, (<i>S</i>)-2-(3,5-dihydroxy-4-isopropylphenyl)-2-guanidinoacetic acid (2), and glycine. A biosynthetic gene cluster was identified in a genome database of <i>Streptoverticillium roseoverticillatum</i> by searching for orthologs of the genes responsible for biosynthesis of pheganomycin (3), which possesses a (2)-derivative at its <i>N</i>-terminus. The cluster contained a gene encoding an ATP-grasp-ligase (<i>res5</i>), which was suggested to catalyze the peptide bond formation between 2 and glycine. A <i>res5</i>-deletion mutant lost 1 productivity but accumulated 2 in the culture broth. However, recombinant RES5 did not show catalytic activity to form 1 with 2 and glycine as substrates. Moreover, heterologous expression of the cluster resulted in accumulation of only 2 and no production of 1 was observed. These results suggested that a peptide with glycine at its <i>N</i>-terminus may be used as a nucleophile and then maturated by a peptidase encoded by a gene outside of the cluster.</p></div

A peptide ligase and the ribosome cooperate to synthesize the peptide pheganomycin

Peptide antibiotics are typically biosynthesized by one of two distinct machineries in a ribosome-dependent or ribosome-independent manner. Pheganomycin (PGM (1)) and related analogs consist of the nonproteinogenic amino acid (S)-2-(3,5-dihydroxy-4-hydroxymethyl)phenyl-2-guanidinoacetic acid (2) and a proteinogenic core peptide, making their origin uncertain. We report the identification of the biosynthetic gene cluster from Streptomyces cirratus responsible for PGM production. Unexpectedly, the cluster contains a gene encoding multiple precursor peptides along with several genes plausibly encoding enzymes for the synthesis of amino acid 2. We identified PGM1, which has an ATP-grasp domain, as potentially capable of linking the precursor peptides with 2, and validate this hypothesis using deletion mutants and in vitro reconstitution. We document PGM1's substrate permissivity, which could be rationalized by a large binding pocket as confirmed via structural and mutagenesis experiments. This is to our knowledge the first example of cooperative peptide synthesis achieved by ribosomes and peptide ligases using a peptide nucleophile

南鳥島EEZ南方海域におけるレアアース資源ポテンシャル評価

http://www.godac.jamstec.go.jp/darwin/cruise/kairei/kr13-02/ehttp://www.godac.jamstec.go.jp/darwin/cruise/kairei/kr14-02/ehttp://www.godac.jamstec.go.jp/darwin/cruise/mirai/mr14-e02/ehttp://www.godac.jamstec.go.jp/darwin/cruise/mirai/mr15-e01_leg2/ehttp://www.godac.jamstec.go.jp/darwin/cruise/mirai/mr15-02/

南鳥島EEZ南部における超高濃度レアアース泥の地球化学的特徴および分布状況

http://www.godac.jamstec.go.jp/darwin/cruise/mirai/mr15-02/