Calculation of new enteric methane emission factors for small ruminants in western Kenya highlights the heterogeneity of smallholder production systems

Context: African livestock play a critical role in food security and the wider economy, while accounting for >70% of African agricultural greenhouse gas emissions. Accurate estimates of greenhouse gas emissions from livestock are required for inventory purposes and to assess the efficacy of mitigation measures. While there is an increasing number of studies assessing methane (CH$_{4}$) emissions of cattle, little attention has been paid to small ruminants (SR). Aims: Enteric CH$_{4}$ emissions were assessed from 1345 SR in three counties of western Kenya to develop more accurate emission factors (EF) for enteric CH$_{4}$ from sheep and goats. Methods: Using on-farm animal activity data, feed samples were also analysed to produce estimates of feed digestibility by season and region. The combined data were also used to estimate daily CH$_{4}$ production by season, location and class of animal to produce new EF for annual enteric CH$_{4}$ production of SR. Key results: Mean dry-matter digestibility of the feed basket was in the range of 58–64%, depending on region and season (~10% greater than Tier I estimates). EF were similar for sheep (4.4 vs 5 kg CH$_{4}$/year), but lower for goats (3.7 vs 5 kg CH$_{4}$/year) than those given for SR in developing countries in Intergovernmental Panel on Climate Change (Tier I) estimates. Conclusions: Published estimates of EF for SR range widely across Africa. In smallholder systems in western Kenya, SR appear to be managed differently from cattle, and EF appear to be driven by different management considerations. Implications: The findings highlighted the heterogenous nature of SR enteric emissions in East Africa, but also suggested that emissions from SR are quantitatively less important than other estimates suggest compared with cattle

Selected Factors Affecting Adoption of Improved Finger Millet Varieties by Small- Scale Farmers in the Semi-Arid Mogotio District, Kenya

Finger millet is one of the important traditional, nutritious and drought tolerant food crop grown by small scale farmers in most arid and semi-arid lands (ASALs) of the world. In the ASALs of Kenya, the small scale farmers mainly grow unimproved finger millet varieties of low yields. Despite availability of improved high yielding and recommended varieties, the farmer

Critical analysis of tillage practices with fertility levels in maize and populations in beans as adaptation measures to climate change to enhance food security at Kabete

Trials were carried out in 2012/2013 short and 2013 long seasons at Kabete site representing a warm and wet environment in Kenya to determine, the appropriateness of combining fertilizer levels for maize and population levels with tied ridges for beans, as adaptation measures under changing climate. The maize experiment consisted of three fertilizer levels of 0, 20 and 40 kg/ha N while the bean experiment had three spacings of 12, 15 and 20 cm in a split plot design. The experiments were replicated thrice and consisted of conventional tillage and tied ridges as main plots representing the two soil water management practices while the three soil fertility levels (in maize N0, N20 and N40) or spacing options (12, 15 and 20cm) were sub plots in a Completely Randomized Block Design. The ridges were tied at intervals of 1 m and spaced at recommended crop spacings (i.e. 75cm for maize and 45cm for beans) and the crop planted on the slope of the ridge in 6 by 5 m plots. Basal phosphate (P205) fertilizer in the form of Triple Superphosphate was applied at planting time at the rate of 40kg/ha. Nitrogen in the form of Calcium Ammonium Nitrate was applied at 20 Kg/ha in the 20 and 40 N treatments at planting and further 20kg/ha N top dressed in the 40 treatment level. Harvesting was done at physiological maturity of grain which was air dried. Statistical analysis was done of the treatments and comparisons done of the adaptation advantages of the treatments. Tied ridging increased maize yields at the medium fertilizer level of 20 (+5.22%) but were negative under both zero (-15.56%) and 40 kg/ha application of fertilizers (-5.42%). In the short season, increased bean spacing from 12 to 20 decreased yields under normal (-13.6%) and tied ridges (-37.3%) but remained higher at populations of 12 and 15. In the long season increasing bean population from spacing of 12 cm to 20 had no advantage and under tied ridging compared to normal tillage. Tied ridging as a climate change adaptive measure should not be instituted as a blanket recommendation across rainfall regimes, crops, fertilization levels or plant populations and is more advantageous in drier seasons

Complementary feeding in the WHO Multicentre Growth Reference Study

Aim: To describe complementary feeding practices in the Multicentre Growth Reference Study (MGRS) sample. Methods: Food frequency questionnaires and 24-h dietary recalls were administered to describe child feeding throughout the first 2 y of life. This information was used to determine complementary feeding initiation, meal frequency and use of fortified foods. Descriptions of foods consumed and dietary diversity were derived from the 24-h recalls. Compliance with the feeding recommendations of the MGRS was determined on the basis of the food frequency reports. Descriptive statistics provide a profile of the complementary feeding patterns among the compliant children. Results: Complementary feeding in the compliant group began at a mean age of 5.4 mo (range: 4.8 (Oman)-5.8 mo (Ghana)). Complementary food intake rose from 2 meals/d at 6 mo to 4-5 meals in the second year, in a reverse trend to breastfeeding frequency. Total intake from the two sources was 11 meals/d at 6-12 mo, dropping to 7 meals/d at 24 mo. Inter-site differences in total meal frequency were mainly due to variations in breastfeeding frequency. Grains were the most commonly selected food group compared with other food groups that varied more by site due to cultural factors, for example, infrequent consumption of flesh foods in India. The use of fortified foods and nutrient supplements was also influenced by site-variable practices. Dietary diversity varied minimally between compliance groups and sites. Conclusion: Complementary diets in the MGRS met global recommendations and were adequate to support physiological growth

Parental Height and Child Growth From Birth to 2 years in the WHO Multicentre Growth Reference Study

inear growth from birth to 2 years of children enrolled in the World Health Organization Multicentre Growth Reference Study was similar despite substantial parental height differences among the six study sites. Within-site variability in child length attributable to parental height was estimated by repeated measures analysis of variance using generalized linear models. This approach was also used to examine relationships among selected traits (e.g. breastfeeding duration and child morbidity) and linear growth between 6 and 24 months of age. Differences in intergenerational adult heights were evaluated within sites by comparing mid-parental heights (average of the mother\u27s and father\u27s heights) to the children\u27s predicted adult height. Mid-parental height consistently accounted for greater proportions of observed variability in attained child length than did either paternal or maternal height alone. The proportion of variability explained by mid-parental height ranged from 11% in Ghana to 21% in India. The average proportion of between-child variability accounted for by mid-parental height was 16% and the analogous within-child estimate was 6%. In the Norwegian and US samples, no significant differences were observed between mid-parental and children\u27s predicted adult heights. For the other sites, predicted adult heights exceeded mid-parental heights by 6.2-7.8 cm. To the extent that adult height is predicted by height at age 2 years, these results support the expectation that significant community-wide advances in stature are attainable within one generation when care and nutrition approximate international recommendations, notwithstanding adverse conditions likely experienced by the previous generation

Correlation of EGFR, pEGFR and p16INK4 expressions and high risk HPV infection in HIV/AIDS-related squamous cell carcinoma of conjunctiva

Background: Squamous cell carcinoma of conjunctiva has increased tenfold in the era of HIV/AIDS. The disease pattern has also changed in Africa, affecting young persons, with peak age-specific incidence of 30-39 years, similar to that of Kaposi sarcoma, a well known HIV/AIDS defining neoplasm. In addition, the disease has assumed more aggressive clinical course. The contributing role of exposure to high risk HPV in the development of SCCC is still emerging. Objective. The present study aimed to investigate if immunohistochemical expressions of EGFR, pEGFR and p16, could predict infection with high risk HPV in HIV-related SCCC. Methods. FFPE tissue blocks of fifty-eight cases diagnosed on hematoxylin and eosin with SCCC between 2005-2011, and subsequently confirmed from medical records to be HIV positive at the department of human pathology, UoN/KNH, were used for the study. Immunohistochemistry was performed to assess the expressions of p16INK4A, EGFR and pEGFR. This was followed with semi-nested PCR based detection and sequencing of HPV genotypes. The sequences were compared with the GenBank database, and data analyzed for significant statistical correlations using SPSS 16.0. Ethical approval to conduct the study was obtained from KNH-ERC. Results: Out of the fifty-eight cases of SCCC analyzed, twenty-nine (50%) had well differentiated (grade 1), twenty one (36.2%) moderately differentiated (grade 2) while eight (13.8%) had poorly differentiated (grade 3) tumours. Immunohistochemistry assay was done in all the fifty eight studied cases, of which thirty nine cases (67.2%) were positive for p16INK4A staining, forty eight cases (82.8%) for EGFR and fifty one cases (87.9%) showed positivity for p-EGFR. HPV DNA was detected in 4 out of 40 SCCC cases (10%) in which PCR was performed, with HPV16 being the only HPV sub-type detected. Significant statistical association was found between HPV detection and p16INK4 (p=0.000, at 99% C.I) and EGFR (p=0.028, at 95% C.I) expressions, but not pEGFR. In addition, the expressions of these biomarkers did not show any significant association with tumor grades. Conclusion: This study points to an association of high risk HPV with over expressions of p16INK4A and EGFR proteins in AIDS-associated SCCC. © 2014 Mwololo et al.; licensee BioMed Central Ltd

Use of a Semi-field System to Evaluate the Efficacy of Topical Repellents under user Conditions Provides a Disease Exposure free Technique Comparable with Field Data.

Before topical repellents can be employed as interventions against arthropod bites, their efficacy must be established. Currently, laboratory or field tests, using human volunteers, are the main methods used for assessing the efficacy of topical repellents. However, laboratory tests are not representative of real life conditions under which repellents are used and field-testing potentially exposes human volunteers to disease. There is, therefore, a need to develop methods to test efficacy of repellents under real life conditions while minimizing volunteer exposure to disease. A lotion-based, 15% N, N-Diethyl-3-methylbenzamide (DEET) repellent and 15% DEET in ethanol were compared to a placebo lotion in a 200 sq m (10 m x 20 m) semi-field system (SFS) against laboratory-reared Anopheles arabiensis mosquitoes and in full field settings against wild malaria vectors and nuisance-biting mosquitoes. The average percentage protection against biting mosquitoes over four hours in the SFS and field setting was determined. A Poisson regression model was then used to determine relative risk of being bitten when wearing either of these repellents compared to the placebo. Average percentage protection of the lotion-based 15% DEET repellent after four hours of mosquito collection was 82.13% (95% CI 75.94-88.82) in the semi-field experiments and 85.10% (95% CI 78.97-91.70) in the field experiments. Average percentage protection of 15% DEET in ethanol after four hours was 71.29% (CI 61.77-82.28) in the semi-field system and 88.24% (84.45-92.20) in the field. Semi-field evaluation results were comparable to full-field evaluations, indicating that such systems could be satisfactorily used in measuring efficacy of topically applied mosquito repellents, thereby avoiding risks of exposure to mosquito-borne pathogens, associated with field testing

Adapting Agriculture to Climate Change: An Evaluation of Yield Potential of Maize, Sorghum, Common Bean and Pigeon Pea Varieties in a Very Cool-Wet Region of Nyandarua County, Central Kenya

Three experiments were conducted to evaluate the performance of maize, sorghum, common bean and pigeon pea varieties under different water management in a cool and wet region of Central Kenya, as a part of the studies at analogue sites. The first experiment evaluated the growth and performance of three varieties (early maturing: EM, medium maturing: MM and late maturing: LM) of maize (Zea mays L), sorghum (Sorghum bicolor L.), pigeon pea (Cajanus cajan) and common bean (Phaseolus vulgaris L.). The second experiment evaluated maize and sorghum response to water conservation and three fertiliser rates (0, 20 and 40 kg N/ha). The third experiment assessed the effect of water conservation measures on crop yields of common bean and pigeon pea grown under three plant densities (low, medium and high). Tied ridge tillage was used as the water conservation measure and disc plough as the control in the second and third experiments. Maize, sorghum, pigeon pea and common bean took more than 180, 245, 217 and 95 days respectively, to reach physiological maturity. The MM maize variety (DK8031), EM pigeon pea variety (ICPL 84091) and LM common bean variety (GLP 24) yielded the greatest grain of 4,938, 881 and 620 kg/ha respectively, among the respective crop varieties. The sorghum varieties were attacked by fungal and rust diseases leading to yield losses in all seasons. Soil water conservation in general did not have a significant effect on crop yield though there were yield improvements. In the plant density trial, the medium plant densities of pigeon pea (33,333 pl/ha) and common bean (148,148 pl/ha) resulted in the greatest grain yields. The highest grain yield of maize (4,184 kg/ha) and sorghum (47 kg/ha) was obtained in plots with 20 kg/ha of nitrogen fertilizer. Based on the results of this study, pigeon pea and common bean can be introduced in the farming systems to improve crop diversity. The production of the tested sorghum varieties should be discouraged in this region because they are prone to fungal and rust diseases due to the cold and wet weather conditions

Adapting agriculture to climate change - An evaluation of yield potential of maize, sorghum, common bean and pigeon pea varieties in a very cool-wet region of Nayandarua County

Soil and water conservation, use of more adaptive crop genotypes and crop diversification are widely accepted as some of the management practices that can help reduce agriculture vulnerability to impacts of climate change. A study was conducted to evaluate the yield potential of maize, sorghum, common bean and pigeon pea varieties under different water management, plant densities and fertility levels in Nyahururu, Central Kenya. The study involved three experiments. The first experiment evaluated the growth and performance of three varieties (early maturing, medium maturing and late maturing) of maize, sorghum, pigeon pea and common bean. The experimental design was a completely randomized block design (RCBD) replicated three times. The second experiment evaluated maize and sorghum yield response to water conservation and three fertiliser rates (0, 20 and 40 kg N/ha). The third experiment assessed the effect of water conservation measures on crop yields of common bean and pigeon pea grown under three plant densities (low, medium and high). Tied ridge tillage was used as the water conservation measure and disc plough as the control in the second and third experiments. Results showed that water conservation in general did not have a significant effect on crop yield though they were improved. The medium density pigeon pea gave the highest grain (719 kg/ha) followed by low (688 kg/ha) and high plant density (687 kg/ha), though not significant at 0.95 confidence level. Similar trends were observed with common bean grain and dry matter yield. Tied ridges tended to lower maize yield compared to flat tillage while it increased sorghum yields but the difference was insignificant. When average across the tillage systems, the highest maize grain (5553 kg/ha) and dry matter (14298 kg/ha) yield was obtained in plots without N fertilizer. Sorghum dry matter was highest (11333 kg/ha) in plots with 40 kg N/ha and lowest (7903 kg/ha) in plots with 20 kg/ha N. In the variety experiment, the EM pigeon pea variety (ICPL 84091) yielded the greatest grain (881 kg/ha) while the late maturing variety (ICEAP 00040) gave the least (565 kg/ha). The LM maize variety (DK8031) yielded the highest grain (5701 kg/ha) and dry matter (18843 kg/ha). The LM sorghum variety (Macia) had 47% and 49% dry matter yield advantage over MM (Kari Mtama 1) and EM (Gadam) varieties, respectively. The yields for common bean varieties tended to vary with seasons. So what are the conclusions

Comparison of the microbial composition of African fermented foods using amplicon sequencing

Fermented foods play a major role in the diet of people in Africa, where a wide variety of raw materials are fermented. Understanding the microbial populations of these products would help in the design of specific starter cultures to produce standardized and safer foods. In this study, the bacterial diversity of African fermented foods produced from several raw materials (cereals, milk, cassava, honey, palm sap, and locust beans) under different conditions (household, small commercial producers or laboratory) in 8 African countries was analysed by 16S rRNA gene amplicon sequencing during the Workshop “Analysis of the Microbiomes of Naturally Fermented Foods Training Course”. Results show that lactobacilli were less abundant in fermentations performed under laboratory conditions compared to artisanal or commercial fermentations. Excluding the samples produced under laboratory conditions, lactobacilli is one of the dominant groups in all the remaining samples. Genera within the order Lactobacillales dominated dairy, cereal and cassava fermentations. Genera within the order Lactobacillales, and genera Zymomonas and Bacillus were predominant in alcoholic beverages, whereas Bacillus and Lactobacillus were the dominant genera in the locust bean sample. The genus Zymomonas was reported for the first time in dairy, cereal, cassava and locust bean fermentations