40 research outputs found
Characterization of cell-specific modulatory element in the murine ornithine decarboxylase promoter
Structure, organization and expression of the mouse ornithine decarboxylase antizyme gene
Androgen receptor uses relaxed response element stringency for selective chromatin binding and transcriptional regulation in vivo
Peer reviewe
Hormonal control of uteroglobin secretion in rabbit uterus. Inhibition of uteroglobin synthesis and messenger ribonucleic acid accumulation by oestrogen and anti-oestrogen administration
Identification of several potential chromatin binding sites of HOXB7 and its downstream target genes in breast cancer
Peer reviewe
Characterization of an androgen-responsive, ornithine decarboxylase-related protein in mouse kidney
Chronic exposure to dexamethasone induces hypomethylation of ornithine decarboxylase genes in a human myeloma cell line
AbstractChronic exposure of a human myeloma cell line to dexamethasone resulted in a selection of cells resistant to the growth-inhibitory action of the glucocorticoid. Upon acute exposure of the parental myeloma cells to dexamethasone growth inhibition was associated with depression of ornithine decarboxylase (ODC, EC 4.1.1.17) activity. However, in cells adapted to grow in the presence of micromolar concentrations of dexamethasone, ODC activity was fully comparable to that in the parental cells. Restriction enzyme analyses with the two isoschizomers HpaII and MspI as well as with the methylation-sensitive C⨍oI, indicated that the otherwise heavily methylated ODC gene(s) were rendered hypomethylated in the myeloma cells resistant to dexamethasone. This hypomethylation within and/or around ODC genes was associated with a 2–4-fold enhancement of accumulation of ODC mRNA