Effects of glazing and chitosan-based coating application on frozen salmon preservation during six-month storage in industrial freezing chambers

Freezing and glazing are techniques commonly used to reduce the incidence of fish deterioration processes. In order to find an alternative to complement freezing and replace water glazing, the present work aimed at evaluating the effect of water glazing and edible coatings of 0.5% w/v and 1.5% w/v chitosan on quality parameters of frozen fish. Both types of coatings - water glazing and chitosan coatings - were applied directly on frozen Atlantic salmon (Salmo salar) and stored for 9 months at -22 °C. Several parameters such as coating/glazing loss, weight loss, drip loss, Total Viable Counts (TVC), Total Volatile Basic-Nitrogen (TVB-N), K-value, pH and color coordinates L*a*b* were periodically evaluated in order to compare glazing with the chitosan-based coatings and uncoated control samples. Samples coated with 1.5% w/v chitosan performed better in maintaining the color of the salmon and controlling microbial contamination of frozen and thawed samples

Impact of the APE1 Redox Function Inhibitor E3330 in Non-Small Cell Lung Cancer Cells Exposed to Cisplatin : Increased Cytotoxicity and Impairment of Cell Migration and Invasion

Elevated expression levels of the apurinic/apyrimidinic endonuclease 1 (APE1) have been correlated with the more aggressive phenotypes and poor prognosis of non-small cell lung cancer (NSCLC). This study aimed to assess the impact of the inhibition of the redox function of APE1 with E3330 either alone or in combination with cisplatin in NSCLC cells. For this purpose, complementary endpoints focusing on cell viability, apoptosis, cell cycle distribution, and migration/invasion were studied. Cisplatin decreased the viability of H1975 cells in a time- and concentration-dependent manner, with IC values of 9.6 µM for crystal violet assay and 15.9 µM for 3-(4,5-Dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium (MTS) assay. E3330 was clearly cytotoxic for concentrations above 30 µM. The co-incubation of E3330 and cisplatin significantly decreased cell viability compared to cisplatin alone. Regarding cell cycle distribution, cisplatin led to an increase in sub-G1, whereas the co-treatment with E3330 did not change this profile, which was then confirmed in terms of % apoptotic cells. In addition, the combination of E3330 and cisplatin at low concentrations decreased collective and chemotactic migration, and also chemoinvasion, by reducing these capabilities up to 20%. Overall, these results point to E3330 as a promising compound to boost cisplatin therapy that warrants further investigation in NSCLC

The evolution of strategy in bacterial warfare via the regulation of bacteriocins and antibiotics.

Bacteria inhibit and kill one another with a diverse array of compounds, including bacteriocins and antibiotics. These attacks are highly regulated, but we lack a clear understanding of the evolutionary logic underlying this regulation. Here, we combine a detailed dynamic model of bacterial competition with evolutionary game theory to study the rules of bacterial warfare. We model a large range of possible combat strategies based upon the molecular biology of bacterial regulatory networks. Our model predicts that regulated strategies, which use quorum sensing or stress responses to regulate toxin production, will readily evolve as they outcompete constitutive toxin production. Amongst regulated strategies, we show that a particularly successful strategy is to upregulate toxin production in response to an incoming competitor's toxin, which can be achieved via stress responses that detect cell damage (competition sensing). Mirroring classical game theory, our work suggests a fundamental advantage to reciprocation. However, in contrast to classical results, we argue that reciprocation in bacteria serves not to promote peaceful outcomes but to enable efficient and effective attacks

Enhancing extraction of food-grade pigments from the microalgae Chlorella Vulgaris through application of ohmic heating

[Excerpt] Introduction: The use of ohmic heating (OH) with the associated non-thermal effects due to the presence of an electrical field and frequency, has been suggested for extraction of compounds from biological matrices. Microalgae are considered as a very valuable source of compounds of interest for food sector (i.e. pigments, lipids, carbohydrates, and proteins) and the selection of extraction technique to recover these compounds is very challenging due to the intrinsic nature of the microalgae cell walls, which limits the mass transfer through it. The main objective of this study is to investigate the effects of OH on the extraction of pigmented solutes from Chlorella vulgaris. [...

Artificial Intelligence in Orthodontics: Where Are We Now? A Scoping Review

Objective: This scoping review aims to determine the applications of Artificial Intelligence (AI) that are extensively employed in the field of Orthodontics, to evaluate its benefits, and to discuss its potential implications in this speciality. Recent decades have witnessed enormous changes in our profession. The arrival of new and more aesthetic options in orthodontic treatment, the transition to a fully digital workflow, the emergence of temporary anchorage devices and new imaging methods all provide both patients and professionals with a new focus in orthodontic care. Materials and methods: This review was performed following the Preferred Reporting Items for Systematic reviews and Meta-Analyses extension for Scoping Reviews (PRISMA-ScR) guidelines. The electronic literature search was performed through MEDLINE/PubMed, Scopus, Web of Science, Cochrane and IEEE Xplore databases with a 11-year time restriction: January 2010 till March 2021. No additional manual searches were performed. Results: The electronic literature search initially returned 311 records, and 115 after removing duplicate references. Finally, the application of the inclusion criteria resulted in 17 eligible publications in the qualitative synthesis review. Conclusion: The analysed studies demonstrated that Convolution Neural Networks can be used for the automatic detection of anatomical reference points on radiological images. In the growth and development research area, the Cervical Vertebral Maturation stage can be determined using an Artificial Neural Network model and obtain the same results as expert human observers. AI technology can also improve the diagnostic accuracy for orthodontic treatments, thereby helping the orthodontist work more accurately and efficiently

The pathogenesis of Staphylococcus epidermidis biofilm-associated infections: the host and the pathogen perspective

Book of Abstracts of CEB Annual Meeting 2017info:eu-repo/semantics/publishedVersio

A Critical Perspective on 3D Liver Models for Drug Metabolism and Toxicology Studies

The poor predictability of human liver toxicity is still causing high attrition rates of drug candidates in the pharmaceutical industry at the non-clinical, clinical, and post-marketing authorization stages. This is in part caused by animal models that fail to predict various human adverse drug reactions (ADRs), resulting in undetected hepatotoxicity at the non-clinical phase of drug development. In an effort to increase the prediction of human hepatotoxicity, different approaches to enhance the physiological relevance of hepatic in vitro systems are being pursued. Three-dimensional (3D) or microfluidic technologies allow to better recapitulate hepatocyte organization and cell-matrix contacts, to include additional cell types, to incorporate fluid flow and to create gradients of oxygen and nutrients, which have led to improved differentiated cell phenotype and functionality. This comprehensive review addresses the drug-induced hepatotoxicity mechanisms and the currently available 3D liver in vitro models, their characteristics, as well as their advantages and limitations for human hepatotoxicity assessment. In addition, since toxic responses are greatly dependent on the culture model, a comparative analysis of the toxicity studies performed using two-dimensional (2D) and 3D in vitro strategies with recognized hepatotoxic compounds, such as paracetamol, diclofenac, and troglitazone is performed, further highlighting the need for harmonization of the respective characterization methods. Finally, taking a step forward, we propose a roadmap for the assessment of drugs hepatotoxicity based on fully characterized fit-for-purpose in vitro models, taking advantage of the best of each model, which will ultimately contribute to more informed decision-making in the drug development and risk assessment fields.This research has been supported by FCT (Portugal) through the research grants and scholarship PTDC/MED-TOX/29183/2017, UIDB/04138/2020, UIDP/04138/2020, and SFRH/BD/144130/2019 to JR and by the H2020, European Commission, though the MSCA-IF-EF-ST – Standard EF to MC (GA-845147-LIV-AD-ON-A-CHIP). Conflict of Interestinfo:eu-repo/semantics/publishedVersio

Pulse wave velocity distribution in a cohort study: from arterial stiffness to early vascular aging

BACKGROUND: By contrast with other southern European people, north Portuguese population registers an especially high prevalence of hypertension and stroke incidence. We designed a cohort study to identify individuals presenting accelerated and premature arterial aging in the Portuguese population. METHOD: Pulse wave velocity (PWV) was measured in randomly sampled population dwellers aged 18-96 years from northern Portugal, and used as a marker of early vascular aging (EVA). Of the 3038 individuals enrolled, 2542 completed the evaluation. RESULTS: Mean PWV value for the entire population was 8.4?m/s (men: 8.6?m/s; women: 8.2?m/s; P??10?m/s). Logistic regression models indicated gender differences concerning the risk of developing large artery damage, with women having the same odds of PWV above 10?m/s 10 years later than men. CONCLUSION: The population PWV values were higher than expected in a low cardiovascular risk area (Portugal). High prevalence rates of EVA and noteworthy large artery damage in young ages were found.Funded by the Life and Health Research Institute, Minho University, Guimarães, Portugal