<i>Schizosaccharomyces pombe</i> Pol II transcription elongation factor ELL functions as part of a rudimentary super elongation complex

ELL family transcription factors activate the overall rate of RNA polymerase II (Pol II) transcription elongation by binding directly to Pol II and suppressing its tendency to pause. In metazoa, ELL regulates Pol II transcription elongation as part of a large multisubunit complex referred to as the Super Elongation Complex (SEC), which includes P-TEFb and EAF, AF9 or ENL, and an AFF family protein. Although orthologs of ELL and EAF have been identified in lower eukaryotes including Schizosaccharomyces pombe, it has been unclear whether SEClike complexes function in lower eukaryotes. In this report, we describe isolation from S. pombe of an ELL-containing complex with features of a rudimentary SEC. This complex includes S. pombe Ell1, Eaf1, and a previously uncharacterized protein we designate Ell1 binding protein 1 (Ebp1), which is distantly related to metazoan AFF family members. Like the metazoan SEC, this S. pombe ELL complex appears to function broadly in Pol II transcription. Interestingly, it appears to have a particularly important role in regulating genes involved in cell separation

Density and magnetic susceptibility relationships in non-magnetic granites; a “wildcard” for modeling potential fields geophysical data

EGU2020: Sharing Geoscience Online, 4-8 May 2020Geophysical surveying (both gravity and magnetic) is of great help in 3D modeling of granitic bodies at depth. As in any potential-field geophysics study, petrophysical data (density [r], magnetic susceptibility [k] and remanence) are of key importance to reduce the uncertainty during the modeling of rock volumes. Several works have already demonstrated that ¿18O or [SiO2] display a negative correlation to density and to magnetic susceptibility. These relationships are particularly stable (and linear) in the so-called ¿non-magnetic¿ granites (susceptibilities falling within the paramagnetic range; between 0 and 500 10-6 S.I.) and usually coincident with calc-alcaline (CA) compositions (very common in Variscan domains). In this work we establish robust correlations between density and magnetic susceptibility at different scales in CA granites from the Pyrenees. Other plutons from Iberia were also considered (Veiga, Monesterio). The main goal is to use the available and densely sampled nets of anisotropy of magnetic susceptibility (AMS) data, performed during the 90¿s and early 2000¿s, together with new data acquired in the last few years, as an indirect measurement of density in order to carry out the 3D modelling of the gravimetric signal. We sampled some sections covering the main range of variability of magnetic susceptibility in the Mont Louis-Andorra, Maladeta and Marimanha granite bodies (Pyrenees), all three characterized by even and dense nets of AMS sites (more than 550 sites and 2500 AMS measurements). We performed new density and susceptibility measurements along two main cross-sections (Maladeta and Mont Louis-Andorra). In these outcrops, numerous measurements (usually more than 50) were taken in the field with portable susceptometers (SM20 and KT20 devices). Density data were derived from the Arquimedes principle applied on large hand samples cut in regular cubes weighting between 0.3 and 0.6 kg (whenever possible). These samples were subsampled and measured later on with a KLY-3 susceptibility bridge in the laboratory. Additionally, some density data were derived from the geometry and weighting of AMS samples. After the calibration of portable and laboratory susceptometers, density and magnetic susceptibility were plotted together. Regressions were derived for every granite body and they usually followed a linear function similar to: r = 2600 kg/m3 + (0.5 * k [10-6 S.I.]). As previously stated, this relationship is only valid in CA and paramagnetic granites, where iron is mostly fractioned in iron-bearing phyllosilicates and the occurrence of magnetite is negligible (or at least its contribution to the bulk susceptibility). These relationships allow transforming magnetic susceptibility data into density data helping in the 3D modelling of the gravimetric signal when density data from rock samples are scarce. Given the large amount of AMS studies worldwide, together with the quickness and cost-effectiveness of susceptibility measurements with portable devices, this methodology allows densifying and homogenizing the petrophysical data when modelling granite rock volumes based on both magnetic and gravimetric signal

Asynchronies during mechanical ventilation are associated with mortality

This study aimed to assess the prevalence and time course of asynchronies during mechanical ventilation (MV). Prospective, noninterventional observational study of 50 patients admitted to intensive care unit (ICU) beds equipped with Better Care (TM) software throughout MV. The software distinguished ventilatory modes and detected ineffective inspiratory efforts during expiration (IEE), double-triggering, aborted inspirations, and short and prolonged cycling to compute the asynchrony index (AI) for each hour. We analyzed 7,027 h of MV comprising 8,731,981 breaths. Asynchronies were detected in all patients and in all ventilator modes. The median AI was 3.41 % [IQR 1.95-5.77]; the most common asynchrony overall and in each mode was IEE [2.38 % (IQR 1.36-3.61)]. Asynchronies were less frequent from 12 pm to 6 am [1.69 % (IQR 0.47-4.78)]. In the hours where more than 90 % of breaths were machine-triggered, the median AI decreased, but asynchronies were still present. When we compared patients with AI > 10 vs AI a parts per thousand currency sign 10 %, we found similar reintubation and tracheostomy rates but higher ICU and hospital mortality and a trend toward longer duration of MV in patients with an AI above the cutoff. Asynchronies are common throughout MV, occurring in all MV modes, and more frequently during the daytime. Further studies should determine whether asynchronies are a marker for or a cause of mortality

Nuevos datos gravimétricos y geológicos de la Zona Axial y límite con la Zona Surpirenaica del Pirineo Central

El conocimiento de la geometría del subsuelo se basa en la integración de datos geológicos y geofísicos. En la Zona Axial del Pirineo Central y sector septentrional de la Zona Surpirenaica, la ausencia de perfiles de sísmica de reflexión dificulta esta labor y hace necesaria la búsqueda de otras técnicas, como la gravimetría, para inferir su estructura en profundidad. En este trabajo los equipos del IGME e ICGC han tomado 1164 nuevas estaciones gravimétricas en la zona de estudio, se han recopilado 2740 estaciones previas de las bases de datos de SITOPO (proyecto TopoIberia) e ICGC y se ha realizado su homogenización. Se ha obtenido un nuevo mapa de la anomalía de Bouguer caracterizado por un mínimo elongado de longitud de onda larga de más de -100 mGal asociado a la raíz cortical de los Pirineos. Cabe destacar la diferente respuesta gravimétrica que ofrecen los dos principales granitos de la zona de estudio, La Maladeta y Andorra-Mont Louis (menor densidad que las rocas Paleozoicas del encajante). Al SO del granito de La Maladeta aparece un mínimo relativo que se interpreta asociado a acumulaciones evaporíticas triásicas (menor densidad) en profundidad. Además se ha realizado: (i) un mapa de anomalía residual asumiendo una anomalía regional correspondiente a un polinomio de tercer grado para inferir la estructura más superficial y (ii) mapas de las derivadas vertical y horizontal de la anomalía gravimétrica residual para observar cómo varían espacialmente los valores de densidad de las rocas existentes. Este trabajo destaca el potencial de la gravimetría en el estudio de la estructura en profundidad de orógenos y cinturones de pliegues y cabalgamientos con presencia de rocas ígneas y/o evaporíticas con valores de densidad menores al de las rocas sedimentarias encajante

Basement and cover architecture in the Central Pyrenees constrained by gravity data

A new gravity survey (1164 gravity stations and 180 samples for density analysis) combined with two new geological cross sections has been carried out in a sector of the Central Pyrenees in order to improve the characterization of basement and cover architecture. From North to South, the study area comprises the southern half of the Axial Zone and the northernmost part of the South-Pyrenean Zone. New gravity data were combined with previous existing databases to obtain the Bouguer and residual anomaly maps of the study area. The two cross sections, oriented NNE–SSW, were built from field data and previous surficial and subsurface data and cross the La Maladeta plutonic complex. The residual anomaly map shows values ranging from −18 to 16 mGal and anomalies mainly oriented N120E. The two 2.5D modelled cross sections show similar observed gravity curves coinciding with similar interpreted structural architecture. Data show a gravity high oriented N120E coinciding with the Orri basement thrust sheet and an important gravity depression, with the same orientation, coinciding with the leading edge at depth of the Rialp basement thrust sheet and interpreted as linked to a large subsurface accumulation of Triassic evaporites. The volume at depth of the La Maladeta and Arties granites has been constrained through gravity modelling. This work highlights that the combination of structural geology and gravity modelling can help to determine the structural architecture of an orogen and localize accumulations of evaporites at depth.This work is part of the project CGL2017-84901-C2-2-P funded by MCIN/AEI/10.13039/501100011033 and “ERDF A way of making Europe” and project PID2020-114273GB-C22 funded by MCIN/AEI/10.13039/501100011033 from Spanish Ministry of Science, Innovation and Universities. Seequent has provided us the GM-SYS module of the Oasis Montaj. The authors acknowledge the contribution of José María Llorente and Agustin González for the acquisition of the gravity data. We thank to Aigüestortes National park and Alt Pirineu Natural park their logistic support. We thank anonymous reviewer for improving the content in the manuscript. This study represents a contribution to GeoAp Research Group (E01-20R) (Aragón Government).Peer reviewe

Estudio de las rocas paleozoicas y los macizos graníticos que afloran en el Parc Natural de l'Alt Pirineu y zonas colindantes mediante datos geológicos, geofísicos y petrofísicos

The Parc Natural de l’Alt Pirineu is located between the La Maladeta and Andorra- Mount Louis granites. The characterization of the Parc’s geology at depth can help to know the emplacement mechanism of these granites, their relationships with the plutonic and volcanic rocks outcropping to the North and South of the Parc, respectively, and if all these rocks belong to the same magmatic system. To achieve these objectives, in the frame of the present research project, we have compiled and homogenezed previous gravimetric and magnetic data, and acquired new geophysical data, in particular, gravity data. We have also constructed new geological cross-sections oriented NE-SW from previous and new acquired geological data and characterized the petrophysical properties of the geological units in order to obtain the density and magnetic susceptibility of all different rocks outcropping in the study area. The geological cross-sections together with the petrophysical data will be used in the 2,5 and 3D gravimetric modelling to improve our understanding of the subsurface geology of this part of the Central Pyrenees and of the Parc Natural de l’Alt Pirineu.El Parc Natural de l’Alt Pirineu se encuentra en la zona situada entre el granito de La Maladeta y el granito de Andorra-Mount Louis. Conocer y entender la geología del subsuelo del Parc nos ayudará a saber cómo se produjo el emplazamiento de ambos macizos graníticos, su relación con las rocas plutónicas y volcánicas que afloran en la parte norte y sur del Parc, respectivamente, y si forman parte de un mismo sistema magmático. Para alcanzar estos objetivos, se está realizando una recopilación y homogeneización de datos gravimétricos y magnéticos ya existentes, así como la adquisición de nuevos datos geofísicos, en particular de gravimetría. Además, partiendo de estudios geológicos previos y datos propios, se está llevando a cabo la realización de cortes geológicos de dirección NE-SO y el estudio petrofísico de las unidades geológicas para obtener los valores de densidad y susceptibilidad magnética de todos los tipos de rocas que afloran en el área de estudio. Los cortes geológicos junto con los datos de petrofísica se utilizarán en la modelización gravimétrica 2,5D y 3D para mejorar la interpretación de la geología del subsuelo en este sector de II Jornades de Recerca del Parc Natural de l‘Alt Pirineu 87 los Pirineos Centrales y del Parc Natural de l’Alt Pirineu.Este trabajo se está llevando a cabo en el marco de los subproyectos CGL2017-84901-C2-1 y CGL2017-84901-C2-2-P del Ministerio de Ciencia, Innovación y Universidades. Los desarrollos metodológicos en exploración con técnicas geofísicas se encuentran alineados con el proyecto europeo 3DGeoEU- GeoERA (ERANET Cofund action 731166 [H2020], Project code GeoE.171.005). Los autores agradecen al Parc Natural de l’Alt Pirineu las facilidades prestadas y la organización de las jornadas de investigación para mostrar los trabajos realizados

Outcomes from elective colorectal cancer surgery during the SARS-CoV-2 pandemic

This study aimed to describe the change in surgical practice and the impact of SARS-CoV-2 on mortality after surgical resection of colorectal cancer during the initial phases of the SARS-CoV-2 pandemic

CM2D3:Furnishing the Human Interactome with Structural Models of Protein Complexes Derived by Comparative Modeling and Docking

The human interactome is composed of around half a million interactions according to recent estimations and it is only for a small fraction of those that three-dimensional structural information is available. Indeed, the structural coverage of the human interactome is very low and given the complexity and time-consuming requirements of solving protein structures this problem will remain for the foreseeable future. Structural models, or predictions, of protein complexes can provide valuable information when the experimentally determined 3D structures are not available. Here we present CM2D3, a relational database containing structural models of the whole human interactome derived both from comparative modeling and data-driven docking. Starting from a consensus interactome derived from integrating several interactomics databases, a strategy was devised to derive structural models by computational means. Currently, CM2D3 includes 33338 structural models of which 5121 derived from comparative modeling and the remaining from docking. Of the latter, the structures of 14554 complexes were derived from monomers modeled by M4T while the rest were modeled with structures as predicted by AlphaFold2. Lastly, CM2D3 complements existing resources by focusing on models derived from both free-docking, as opposed to template-based docking, and hence expanding the available structural information on protein complexes to the scientific community