INFERINDO RELAÇÕES FILOGENÉTICAS A PARTIR DA CONSERVAÇÃO DA PROTEÍNA HOMEÓTICA HOXA-1 DE DIFERENTES VERTEBRADOS POR ANÁLISES GENÉTICAS EVOLUTIVAS MOLECULARES

O objetivo do presente estudo foi investigar as relações filogenéticas entre vertebrados a partir de diferenças na natureza química dos resíduos de aminoácidos da proteína homeótica HoxA por análises comparativas de alinhamentos múltiplos. Após a busca e seleção das sequências da proteína HoxA de 18 animais em um banco de dados de informação biológica, alinhamentos múltiplos de seus resíduos foram efetuados empregando o programa Clustal Omega, seguido pela construção de um dendograma para inferir as relações filogenéticas entre as espécies selecionadas. Análises comparativas dos alinhamentos múltiplos revelaram alta conservação estrutural com valor de identidade máxima de 35,593% entre os aminoácidos da proteína HoxA de distintos vertebrados. Além disso, o dendograma das relações filogenéticas demonstrou o agrupamento dos vertebrados selecionados dispostos em diferentes sub-ramos, onde animais com alta proximidade evolutiva foram intimamente agrupados em alguns sub-ramos, tais como: camundongo e ratazana (0,02) e homem e chimpanzé (0,01). Entretanto, divergências evolutivas em animais com alta proximidade evolutiva também foram observadas com o agrupamento de peixe-zebra (0,46), tubarão chifre (0,17) e celacanto (0,15) em ramos mais divergentes. Assim, conservação estrutural e proximidade filogenética descritas no presente estudo fornecem subsídios acerca da história evolutiva de vertebrados com base nas relações filogenéticas geradas a partir de alinhamentos múltiplos de aminoácidos da proteína HoxA de diferentes animais

Understanding the relation between Zika virus infection during pregnancy and adverse fetal, infant and child outcomes: a protocol for a systematic review and individual participant data meta-analysis of longitudinal studies of pregnant women and their infants and children

IntroductionZika virus (ZIKV) infection during pregnancy is a known cause of microcephaly and other congenital and developmental anomalies. In the absence of a ZIKV vaccine or prophylactics, principal investigators (PIs) and international leaders in ZIKV research have formed the ZIKV Individual Participant Data (IPD) Consortium to identify, collect and synthesise IPD from longitudinal studies of pregnant women that measure ZIKV infection during pregnancy and fetal, infant or child outcomes.Methods and analysisWe will identify eligible studies through the ZIKV IPD Consortium membership and a systematic review and invite study PIs to participate in the IPD meta-analysis (IPD-MA). We will use the combined dataset to estimate the relative and absolute risk of congenital Zika syndrome (CZS), including microcephaly and late symptomatic congenital infections; identify and explore sources of heterogeneity in those estimates and develop and validate a risk prediction model to identify the pregnancies at the highest risk of CZS or adverse developmental outcomes. The variable accuracy of diagnostic assays and differences in exposure and outcome definitions means that included studies will have a higher level of systematic variability, a component of measurement error, than an IPD-MA of studies of an established pathogen. We will use expert testimony, existing internal and external diagnostic accuracy validation studies and laboratory external quality assessments to inform the distribution of measurement error in our models. We will apply both Bayesian and frequentist methods to directly account for these and other sources of uncertainty.Ethics and disseminationThe IPD-MA was deemed exempt from ethical review. We will convene a group of patient advocates to evaluate the ethical implications and utility of the risk stratification tool. Findings from these analyses will be shared via national and international conferences and through publication in open access, peer-reviewed journals.Trial registration numberPROSPERO International prospective register of systematic reviews (CRD42017068915).</jats:sec

Mycological diversity description II

Here, Diaporthe myracrodruonis is introduced as new species from Brazil, isolated as endophyte from Myracrodruon urundeuva. Asterina mandaquiensis is epitypified and ilustrated for the first time. Serpula similis is reported as new to the Neotropics, while Perenniporia centrali-africana is reported for the first time as endophyte and Preussia africana as endophyte from Spondias tuberosa in Caatinga in Brazil

Mycological Diversity Description II

ABSTRACT Here, Diaporthe myracrodruonis is introduced as new species from Brazil, isolated as endophyte from Myracrodruon urundeuva. Asterina mandaquiensis is epitypified and ilustrated for the first time. Serpula similis is reported as new to the Neotropics, while Perenniporia centrali-africana is reported for the first time as endophyte and Preussia africana as endophyte from Spondias tuberosa in Caatinga in Brazil