Byt’ y imet’ en la lógica y en la lingüística

В статье рассматриваются базовые для организации языковых и мыслительных структур отношения тождества и обладания, которые выражаются с помощью именных форм сказуемого с эксплицитно или имплицитно представленными глаголами быть и иметь. Исследование показало, что данные конструкции не только тесно связаны между собой в структурном отношении, они обладают единой природой категориальных значений и составляют основу для формирования многих других видов синтаксической связи.This study investigates the basic for language and logical thinking structures relations of identity and possession, which are expressed by means of compound nominal predicates with the explicit or implicit forms of the present time verbs to be and to have. The research showed that these constructions not only are closely connected among themselves in relation to their structural properties, but have the same categorical values and make a base for formation of many other types of syntactic relations

LITHUANIA`S ROOTS IN THE POTTERY OF THE WESTERN SUBURBS OF MOSCOW OF THE 17TH AND 18TH CENTURIES

The article looks at possible ways and origins of cultural influence by the example of the pottery production of the New Jerusalem Monastery in the 17th and 18th centuries. It tells about the pottery of the New Jerusalem Monastery, and an attempt is made to trace signs of the influence of different craftsmen, and the situation in general for the development of pottery production and its special features. DOI: http://dx.doi.org/10.15181/ab.v16i0.38 Key words: New Jerusalem Monastery, pottery, tableware, thrown pottery, glaze, engobe, origin, Lithuania

Application of ESI FT-ICR MS to Study Kraft Lignin Modification by the Exoenzymes of the White Rot Basidiomycete Fungus TrametesHirsutaLE-BIN 072

Trameteshirsuta is a wood rotting fungus that possesses a vast array of lignin degrading enzymes, including7 laccases, 7 ligninolyticmanganese peroxidases, 9 lignin peroxidases and 2 versatile peroxidases. In this study,electrospray ionization Fourier transform ion cyclotron resonance mass spectrometry (ESI FT-ICR MS)was used to examine kraft lignin modification by the enzymatic system of this fungus.The observed pattern of lignin modification suggested that before the 6th day of cultivation,the fungal enzymatic system tended to degrade more oxidized moleculesand, hence, less recalcitrant molecules, with the production of hard-to-modify reduced molecular species. At some point after the 6th day of cultivation,the fungal enzymatic system tended to degrade more oxidized moleculesand, hence, less recalcitrant molecules, with the production of hard-to-modify reduced molecular species. At some point after the 6th day of cultivation,the fungus started to degrade less oxidized, more recalcitrant, compounds, converting them into the more oxidized forms. The altered pattern of lignin modification enabled changes in the fungal enzymatic system. These changes were further attributed to the appearance of the particular ligninolyticmanganese peroxides enzyme(MnP7), which was added by the fungus to the mixture of enzymes that had already been secreted (VP2 and MnP5). Keywords: wood rotting fungi, kraft lignin, mass spectrometry, peroxidase

Biodegradation Potential of SteccherinumOchraceum: Growth on Different Wood Types and Preliminary Evaluation of Enzymatic Activities

White-rot fungi isa source of a great variety of oxidative and hydrolytic enzymes suitable for biotechnological applications, e.g. in pulp and paper, textile and food industries, bioethanol production, degradation of recalcitrant environmental pollutants,and others. Steccherinumochraceum is a xylotrophicwhite-rot basidiomycetethat can be found in variousclimatic zones on different woody substrates (mostly well decayed). For this research, seventeenstrains of S. ochraceumwere collected in different regions of Russia from various wood substrates (aspen, alder, oak, hazel, birch and willow). Phylogeneticanalyseswere performedbasedon the nucleotide sequences of ITS1, ITS2, 5.8S rRNA, 28S rRNA, β-tubulin and tef1.Oxidaseandcellulaseactivitieswereassessedbyplate-tests with ABTS and CMC. Forevaluation of biodegradation potential,solid state fermentation on alder and pine sawdust wasperformed. Weightanddensitylossaswellas the C:Nratioweremeasuredafter 90 days of cultivation.All S. ochraceum strains exhibited high oxidative activity towards ABTS, indicating secretion of oxidative enzymes (i.e. laccases and class II peroxidases). Cellulase activity was medium or low for most strains and in some strains – absent. Allstrainswereabletodegradealderandpinesawdust. There was no correlation between the enzymatic activity, biodegradation potential and geographic origin of S. ochraceum strains. However, S. ochraceum strains isolated from the same wood substrates exhibited similar characteristics in most cases. Strain LE-BIN 3398 was the most effective for degrading both alder and pine sawdust and could be regarded as a promising source of oxidative enzymes for biotechnology. Keywords: basidiomycetes, biodegradation, solid state fermentation, oxidase activity, Steccherinumochraceu

Coxiella burnetii Genotyping

Multispacer sequence typing is the first reliable method for typing Coxiella burnetii isolates

Luminescent SiO2 nanoparticles for cell labeling: combined water dispersion polymerization and 3D condensation controlled by oligoperoxide surfactant-initiator

Hybrid polymer coated silica nanoparticles (NPs) were synthesized using low temperature graft (co)polymerization of trimethoxysilane propyl methacrylate (MPTS) initiated by surface-active oligoperoxide metal complex (OMC) in aqueous media. These NPs were characterized by means of kinetic, solid-state NMR, TEM and FTIR techniques. Two processes, namely the radical graft-copolymerization due to presence of double bonds and 3D polycondensation provided by the intra- or/and intermolecular interaction of organosilicic fragments, occurred simultaneously. The relative contribution of the reactions depending on initiator concentration and pH value leading to the formation of low cured polydisperse microparticles or OMC coated SiO2 NPs of controlled curing degree was studied. The availability of free-radical forming peroxide fragments on the surface of SiO2 NPs provides an opportunity for seeded polymerization leading to the formation of the functional polymer coated NPs with controlled particle structure, size, and functionality. Encapsulation of the luminescent dye (Rhodamine 6G) in SiO2 core of functionalized NPs provided a noticeable increase in their resistance to photo-bleaching and improved biocompatibility. These luminescent NPs were not only attached to murine leukemia L1210 cells but also tolerated by the mammalian cells. Their potential use for labeling of the mammalian cells is considered

Catalytic Efficiency of Basidiomycete Laccases: Redox Potential versus Substrate-Binding Pocket Structure

Laccases are copper-containing oxidases that catalyze a one-electron abstraction from various phenolic and non-phenolic compounds with concomitant reduction of molecular oxygen to water. It is well-known that laccases from various sources have different substrate specificities, but it is not completely clear what exactly provides these differences. The purpose of this work was to study the features of the substrate specificity of four laccases from basidiomycete fungi Trametes hirsuta, Coriolopsis caperata, Antrodiella faginea, and Steccherinum murashkinskyi, which have different redox potentials of the T1 copper center and a different structure of substrate-binding pockets. Enzyme activity toward 20 monophenolic substances and 4 phenolic dyes was measured spectrophotometrically. The kinetic parameters of oxidation of four lignans and lignan-like substrates were determined by monitoring of the oxygen consumption. For the oxidation of the high redox potential (>700 mV) monophenolic substrates and almost all large substrates, such as phenolic dyes and lignans, the redox potential difference between the enzyme and the substrate (ΔE) played the defining role. For the low redox potential monophenolic substrates, ΔE did not directly influence the laccase activity. Also, in the special cases, the structure of the large substrates, such as dyes and lignans, as well as some structural features of the laccases (flexibility of the substrate-binding pocket loops and some amino acid residues in the key positions) affected the resulting catalytic efficiency

Lietuviškos šaknys XVII-XVIII a. vakarinių Maskvos apylinkių miestų keramikoje

Straipsnis yra skirtas XVII-XVIII a. keramikos, rastos Naujosios Jeruzalės vienuolyne, kuris yra Istros (40 km į vakarus nuo Maskvos) mieste, galimoms kultūrinės įtakos kilmės paieškoms (1 pav.). Straipsnyje taip pat aptariama įvairių meistrų įtaka ir bendra keramikos, rastos Naujosios Jeruzalės vienuolyne, raida bei jos specifiniai bruožai. XVII-XVIII a. virtuviniai indai, rasti Istroje, yra gaminti iš raudonojo molio ir priklauso dviem pagrindiniams tipams. Pirmojo tipo keramika yra būdinga vakariniams Maskvos apylinkių miestams. Antrojo tipo virtuvinių indų kilmė iki šiol neaiški. Tačiau galima manyti, kad šis tipas yra susijęs su regionu, kuriame buvo įprasta keramikinius indus gaminti iš baltojo molio. Stalo indai, kurie yra padengti glazūra ir angobu, tokie kaip indų dangčiai, keptuvės, dubenys, turi tiesioginių analogijų Klaipėdos (Lietuva) miesto keramikoje ir apskritai Hanzos miestų sąjungos keramikoje. Neįprastai didelis kiekis glazūra dengtos keramikos, glazūros, kuri dengia indus ir koklius, identiškumas, kaip ir tapatus dekoras ir didelė jo įvairovė, įgalina teigti, kad ši keramika buvo gaminta tose pačiose dirbtuvėse (2–9 pav.)

Purification and Characterization of Two Novel Laccases from Peniophora lycii

Although, currently, more than 100 laccases have been purified from basidiomycete fungi, the majority of these laccases were obtained from fungi of the Polyporales order, and only scarce data are available about the laccases from other fungi. In this article, laccase production by the white-rot basidiomycete fungus Peniophora lycii, belonging to the Russulales order, was investigated. It was shown that, under copper induction, this fungus secreted three different laccase isozymes. Two laccase isozymes—Lac5 and LacA—were purified and their corresponding nucleotide sequences were determined. Both purified laccases were relatively thermostable with periods of half-life at 70 °C of 10 and 8 min for Lac5 and LacA, respectively. The laccases demonstrated the highest activity toward ABTS (97 U·mg−1 for Lac5 and 121 U·mg−1 for LacA at pH 4.5); Lac5 demonstrated the lowest activity toward 2,6-DMP (2.5 U·mg−1 at pH 4.5), while LacA demonstrated this towards gallic acid (1.4 U·mg−1 at pH 4.5). Both Lac5 and LacA were able to efficiently decolorize such dyes as RBBR and Bromcresol Green. Additionally, phylogenetic relationships among laccases of Peniophora spp. were reconstructed, and groups of orthologous genes were determined. Based on these groups, all currently available data about laccases of Peniophora spp. were systematized