One Health Approach: An Overview of Q Fever in Livestock, Wildlife and Humans in Asturias (Northwestern Spain)

This study aimed to investigate the seroprevalence of C. burnetii in domestic ruminants, wild ungulates, as well as the current situation of Q fever in humans in a small region in northwestern Spain where a close contact at the wildlife–livestock–human interface exists, and information on C. burnetii infection is scarce. Seroprevalence of C. burnetii was 8.4% in sheep, 18.4% in cattle, and 24.4% in goats. Real-time PCR analysis of environmental samples collected in 25 livestock farms detected Coxiella DNA in dust and/or aerosols collected in 20 of them. Analysis of sera from 327 wild ungulates revealed lower seroprevalence than that found in domestic ruminants, with 8.4% of Iberian red deer, 7.3% chamois, 6.9% fallow deer, 5.5% European wild boar and 3.5% of roe deer harboring antibodies to C. burnetii. Exposure to the pathogen in humans was determined by IFAT analysis of 1312 blood samples collected from patients admitted at healthcare centers with Q fever compatible symptoms, such as fever and/or pneumonia. Results showed that 15.9% of the patients had IFAT titers ≥ 1/128 suggestive of probable acute infection. This study is an example of a One Health approach with medical and veterinary institutions involved in investigating zoonotic diseasesThis work was funded by INIA—Spanish National Institute for Agricultural and Food Research and Technology (RTA2017-00055-C02-02), the European Regional Development Funds (ERDF), and PCTI 2018–2020 (GRUPIN: IDI2018-000237)S

Spatial distribution and risk factors of Brucellosis

Background: The role of wildlife as a brucellosis reservoir for humans and domestic livestock remains to be properly established. The aim of this work was to determine the aetiology, apparent prevalence, spatial distribution and risk factors for brucellosis transmission in several Iberian wild ungulates. Methods: A multi-species indirect immunosorbent assay (iELISA) using Brucella S-LPS antigen was developed. In several regions having brucellosis in livestock, individual serum samples were taken between 1999 and 2009 from 2,579 wild bovids, 6,448 wild cervids and4,454 Eurasian wild boar (Sus scrofa), and tested to assess brucellosis apparent prevalence. Strains isolated from wild boar were characterized to identify the presence of markers shared with the strains isolated from domestic pigs. Results: Mean apparent prevalence below 0.5% was identified in chamois (Rupicapra pyrenaica), Iberian wild goat (Capra pyrenaica), and red deer (Cervus elaphus). Roe deer (Capreolus capreolus), fallow deer (Dama dama), mouflon (Ovis aries) and Barbary sheep (Ammotragus lervia) tested were seronegative. Only one red deer and one Iberian wild goat resulted positive in culture, isolating B. abortus biovar 1 and B. melitensis biovar 1, respectively. Apparent prevalence in wild boar ranged from 25% to 46% in the different regions studied, with the highest figures detected in South-Central Spain. The probability of wild boar being positive in the iELISA was also affected by age, age-by-sex interaction, sampling month, and the density of outdoor domestic pigs. A total of 104 bacterial isolates were obtained from wild boar, being all identified as B. suis biovar 2. DNA polymorphisms were similar to those found in domestic pigs. Conclusions: In conclusion, brucellosis in wild boar is widespread in the Iberian Peninsula, thus representing an important threat for domestic pigs. By contrast, wild ruminants were not identified as a significant brucellosis reservoir for livestock

Sarcoptic mange in wild ruminants in Spain : solving the epidemiological enigma using microsatellite markers

In Spain, sarcoptic mange was first described in native wildlife in 1987 in Cazorla Natural Park, causing the death of nearly 95% of the local native population of Iberian ibex (Capra pyrenaica). Since then, additional outbreaks have been identified in several populations of ibex and other wild ungulate species throughout the country. Although the first epizootic outbreak in wildlife was attributed to the introduction of an infected herd of domestic goats, the origin and the cause of its persistence remain unclear. The main aims of this study are to understand (i) the number of Sarcoptes scabiei " strains" circulating in wild ruminant populations in Spain, and (ii) the molecular epidemiological relationships between S. scabiei and its hosts. Ten Sarcoptes microsatellite markers were used to characterize the genetic structure of 266 mites obtained from skin scrapings of 121 mangy wild ruminants between 2011 and 2019 from 11 areas in Spain. Seventy-three different alleles and 37 private alleles were detected. The results of this study show the existence of three genetic strains of S. scabiei in the wild ruminant populations investigated. While two genetic clusters of S. scabiei were host- and geography-related, one cluster included multi-host mites deriving from geographically distant populations. The molecular epidemiological study of S. scabiei in wild ruminants in Spain indicates that the spreading and persistence of the parasite may be conditioned by host species community composition and the permissiveness of each host population/community to the circulation of individual "strains," among other factors. Wildlife-livestock interactions and the role of human-driven introduction or trade of wild and domestic animals should be better investigated to prevent further spread of sarcoptic mange in as yet unaffected natural areas of the Iberian Peninsula. The online version contains supplementary material available at 10.1186/s13071-021-04673-x

Ethnic mirrors: self-representations in the Welsh and Mennonite museums in Argentina and Paraguay

According to some scholars and philosophers, ethnic identities are the best political, social, economic, ethic (and even aesthetic) alternative to State centralism, which is incapable of dealing with cultural diversity. Ethnic communitarism is then defined as a more authentic, humane, democratic and inclusive form of organization. The Welsh colonies of Chubut (Argentine) and the established Mennonite colonies of the Chaco Region (Paraguay) are two ethnic groups with forms of community life that have been thoroughly studied from different perspectives. However, neither has been analyzed their point of view of alterity or their relation with those who do not belong to the community. In their museums the history of the community is represented, self-images and other people's images are constructed and spread. The interesting part of these stories is not what they say but what they do, the form in which contents are expressed. These communitarian historical museums tell about the past but they mainly have an impact on the present. Like national or even imperial museums, Welsh and Mennonite museums tend to naturalize a particular self-centered, prejudicial and evolutionist point of view that often excludes other perspectives, especially those elaborated by the neighboring indigenous communities. In contrast, we believe it is necessary to take a stance for democratic, horizontal relations between communities and more polyphonic and responsible historical representations.Alguns filósofos e acadêmicos assinalam que as identidades étnicas são a melhor alternativa política, social, econômica, ética (e mesmo estética) ao centralismo estatal, que é negligente ao lidar com a diversidade cultural. O comunitarismo étnico é definido como uma forma de organização mais autêntica, humana, democrática e inclusiva. As colônias galesas de Chubut (Argentina) e as colônias rurais dos menonitas no Chaco (Paraguay) são dois grupos étnicos cujas vidas comunitárias têm sido muito estudadas desde diversas perspectivas, mas o seu ponto de vista acerca da alteridade ou sua relação com os atores extracomunitários nunca foi levado em conta. A historia comunitária é representada nos museus galeses e menonitas das respectivas regiões e aí são construídas e difundidas não só as autoimagens mas também as representações dos outros. O aspecto relevante dessas histórias não é o que elas dizem, mas o que fazem, a forma como os conteúdos são expressos. Esses museus históricos comunitários falam sobre o passado, mas seu maior impacto recai sobre o presente. Como os museus nacionais ou imperiais, os museus galeses e menonitas tentam naturalizar pontos de vista particulares, auto-centrados, preconceituosos e evolucionistas que geralmente excluem as perspectivas elaboradas pelas comunidades indígenas vizinhas. Em vez disso, pensamos ser necessário criar relações mais horizontais e democráticas entre as comunidades e difundir representações históricas mais polifônicas e responsáveis

Genetic epidemiology of Sarcoptes scabiei in the Iberian wolf in Asturias, Spain

[Background]: During the last decades, attempts have been made to understand the molecular epidemiology of Sarcoptes scabiei, and to detect and clarify the differences between isolates from different hosts and geographic regions. Two main phenomena have been described: (i) host-taxon derived-Sarcoptes mite infection in European wild animals (revealing the presence of three separate clusters, namely herbivore-, carnivore- and omnivore-derived Sarcoptes populations in Europe) and (ii) prey-to-predator Sarcoptes mite infection in the Masai Mara ecosystem.[Results]: Using one multiplex of 9 microsatellite markers and Sarcoptes mite samples from sympatric Pyrenean chamois, red deer, red fox and Iberian wolf, different population structure analyses revealed concordance with the host-taxon law described for wild animals in Europe, with two main host-derived Sarcoptes mite populations, herbivore- and carnivore-derived. Surprisingly, Iberian wolf derived Sarcoptes populations had the highest genetic diversity among the other populations, including two different subpopulations: one similar to the herbivore-derived Sarcoptes populations, and another similar to carnivore (fox)-derived Sarcoptes mite population.[Conclusions]: The host-taxon effect in wild animals is still supported with the maintenance of carnivore- and herbivore-derived Sarcoptes clusters’ separation in analyzed mites. However, this phenomenon could be modified with the inclusion of a large predator as wolf in the present work, revealing prey-to-predator Sarcoptes mite infection between the studied host-taxa and suggesting the importance of wolf's immune system for explaining the high variability reported in C. lupus derived mites. Further studies of host diet, behavior and movement, and regarding the role played by its immune system, would be of great help to clarify interactions between the two hypotheses, host-taxon and prey-to-predator.This is a contribution to the agreement between CSIC and Principado de Asturias. The research was partially supported by RNM-6400, Proyecto de Excelencia (Junta de Andalucia, Spain), Project RTA 2011-00087-00-00 (INIA), and Juan de la Cierva Grant to Dr S. Alasaad.Peer reviewe

Sarcoptic mange in wild ruminants in Spain : solving the epidemiological enigma using microsatellite markers

In Spain, sarcoptic mange was first described in native wildlife in 1987 in Cazorla Natural Park, causing the death of nearly 95% of the local native population of Iberian ibex (Capra pyrenaica). Since then, additional outbreaks have been identified in several populations of ibex and other wild ungulate species throughout the country. Although the first epizootic outbreak in wildlife was attributed to the introduction of an infected herd of domestic goats, the origin and the cause of its persistence remain unclear. The main aims of this study are to understand (i) the number of Sarcoptes scabiei " strains" circulating in wild ruminant populations in Spain, and (ii) the molecular epidemiological relationships between S. scabiei and its hosts. Ten Sarcoptes microsatellite markers were used to characterize the genetic structure of 266 mites obtained from skin scrapings of 121 mangy wild ruminants between 2011 and 2019 from 11 areas in Spain. Seventy-three different alleles and 37 private alleles were detected. The results of this study show the existence of three genetic strains of S. scabiei in the wild ruminant populations investigated. While two genetic clusters of S. scabiei were host- and geography-related, one cluster included multi-host mites deriving from geographically distant populations. The molecular epidemiological study of S. scabiei in wild ruminants in Spain indicates that the spreading and persistence of the parasite may be conditioned by host species community composition and the permissiveness of each host population/community to the circulation of individual "strains," among other factors. Wildlife-livestock interactions and the role of human-driven introduction or trade of wild and domestic animals should be better investigated to prevent further spread of sarcoptic mange in as yet unaffected natural areas of the Iberian Peninsula. The online version contains supplementary material available at 10.1186/s13071-021-04673-x