Spectroscopic studies of individual plasmon resonant nanoparticles

We present a detailed description of the apparatus and techniques that we have utilized in our experimental study of individual plasmon resonant nanoparticles, along with a brief description of some major results. The apparatus consists of a spectroscopic system combined with a modified darkfield microscope, which enables the user to sequentially select individual resonant nanostructures in the microscopic field of view for spectroscopic study. Plasmon resonant nanostructures scatter light elastically, and typically have very large scattering cross-sections at their resonant optical wavelengths. In general, spectra can be obtained with acquisition times between .1 to 30 seconds, and color images can be captured using consumer digital color cameras. Spheres, tetrahedrons, and pentagonal platelets were fabricated using colloidal chemistry techniques. To produce highly anisotropic structures such as nanorods and "barbells", templates were used. Many of these nanostructures have been individually spectroscopically characterized, and their spectra correlated with their shape and size as determined by transmission electron microscope (TEM). The unique shape, size, composition, and dielectric surroundings of the individual plasmon resonant nanostructures determine their plasmon resonant behavior. We will show how the composition of the substrate on which the particles are immobilized and the dielectric of the surrounding medium have a significant effect on the plasmon resonance of the individual particles

A novel route for identifying starch diagenetic products in the archaeological record

This work introduces a novel analytical chemistry method potentially applicable to the study of archaeological starch residues. The investigation involved the laboratory synthesis of model Maillard reaction mixtures and their analysis through Fourier-Transform Ion Cyclotron Resonance Mass Spectrometry (FTICR-MS). Thus, starch from sixteen plant species were matured while reacting it with the amino acid glycine. The FTICR-MS analysis revealed > 5,300 molecular compounds, with numerous unique heteroatom rich compound classes, ranging from 20 (Zea mays) to 50 (Sorghum bicolor). These classes were investigated as repositories of chemical structure retaining source and process-specific character, linked back to botanical provenance. We discussed the Maillard reaction products thus generated, a possible pathway for the preservation of degraded starch, while also assessing diagenetic recalcitrance and adsorption potential to mineral surfaces. In some cases, hydrothermal experimentation on starches without glycine reveals that the chemical complexity of the starch itself is sufficient to produce some Maillard reaction products. The article concludes that FTICR-MS offers a new analytical window to characterize starchy residue and its diagenetic products, and is able to recognize taxonomic signals with the potential to persist in fossil contexts.Introduction Materials and methods - Sample preparation and characterization - FTICR-MS analysis Results - Characterization of Maillard reaction products based on atomic ratios (H/C, O/C, N/C, N/O) and compound class distribution - Variations in molecular distribution Discussion - The Maillard reaction products - Preservation pathway - Diagenetic recalcitrance of Maillard reaction products Conclusion

Cytotoxic clinical isolates of Pseudomonas aeruginosa identified during the Steroids for Corneal Ulcers Trial show elevated resistance to fluoroquinolones.

BackgroundTo determine the relationship between type three secretion genotype and fluoroquinolone resistance for P. aeruginosa strains isolated from microbial keratitis during the Steroids for Corneal Ulcers Trial (SCUT) and for two laboratory strains, PA103 and PAO1.MethodsConfirmed P. aeruginosa isolates from the SCUT were divided into exoU(+) or exoU(-). The exoU(+) strains contained the gene encoding ExoU, a powerful phospholipase toxin delivered into host cells by the type three secretion system. Isolates were then assessed for susceptibility to fluoroquinolone, cephalosporin, and aminoglycoside antibiotics using disk diffusion assays. Etest was used to determine the MIC of moxifloxacin and other fluoroquinolones. Laboratory isolates in which the exoU gene was added or deleted were also tested.ResultsA significantly higher proportion of exoU(+) strains were resistant to ciprofloxacin (p = 0.001), gatifloxacin (p = 0.003), and ofloxacin (p = 0.002) compared to exoU(-) isolates. There was no significant difference between exoU(+) or exoU(-) negative isolates with respect to susceptibility to other antibiotics except gentamicin. Infections involving resistant exoU(+) strains trended towards worse clinical outcome. Deletion or acquisition of exoU in laboratory isolates did not affect fluoroquinolone susceptibility.ConclusionsFluoroquinolone susceptibility of P. aeruginosa isolated from the SCUT is consistent with previous studies showing elevated resistance involving exoU encoding (cytotoxic) strains, and suggest worse clinical outcome from infections involving resistant isolates. Determination of exoU expression in clinical isolates of P. aeruginosa may be helpful in directing clinical management of patients with microbial keratitis

Recent Results on Strangeness Production at RHIC

Due to its large acceptance, the STAR experiment has acquired a wealth of data on strangeness production for a variety of collisions systems and energies, from p+p to Au+Au. By using the yields and spectra, we address the evolution of the bulk system, including strangeness enhancement and the flavour dependence of radial and elliptic flow. Utilising the fact that we can identify strange baryons and mesons, we investigate different hadronization mechanisms in the intermediate and high p$_{T}$ regions. The ratios of the particle yields, measured to high p$_{T}$, are used to further investigate the range and applicability of the previously reported anomalous baryon production. We also study two-particle azimuthal correlations of identified particles in order to investigate any flavour dependence of jet fragmentation in the available p$_{T}$ range. Data was presented for a number of different collision systems and energies.Comment: Proceedings of SQM'06 Conference, LA, 2006 (submitted to J. Phys. G

Cytotoxic Clinical Isolates of Pseudomonas Aeruginosa Identified During the Steroids for Corneal Ulcers Trial Show Elevated Resistance to Fluoroquinolones

Background: To determine the relationship between type three secretion genotype and fluoroquinolone resistance for P. aeruginosa strains isolated from microbial keratitis during the Steroids for Corneal Ulcers Trial (SCUT) and for two laboratory strains, PA103 and PAO1. Methods: Confirmed P. aeruginosa isolates from the SCUT were divided into exoU(+) or exoU(−). The exoU(+) strains contained the gene encoding ExoU, a powerful phospholipase toxin delivered into host cells by the type three secretion system. Isolates were then assessed for susceptibility to fluoroquinolone, cephalosporin, and aminoglycoside antibiotics using disk diffusion assays. Etest was used to determine the MIC of moxifloxacin and other fluoroquinolones. Laboratory isolates in which the exoU gene was added or deleted were also tested. Results: A significantly higher proportion of exoU(+) strains were resistant to ciprofloxacin (p = 0.001), gatifloxacin (p = 0.003), and ofloxacin (p = 0.002) compared to exoU(−) isolates. There was no significant difference between exoU(+) or exoU(−) negative isolates with respect to susceptibility to other antibiotics except gentamicin. Infections involving resistant exoU(+) strains trended towards worse clinical outcome. Deletion or acquisition of exoU in laboratory isolates did not affect fluoroquinolone susceptibility. Conclusions: Fluoroquinolone susceptibility of P. aeruginosa isolated from the SCUT is consistent with previous studies showing elevated resistance involving exoU encoding (cytotoxic) strains, and suggest worse clinical outcome from infections involving resistant isolates. Determination of exoU expression in clinical isolates of P. aeruginosa may be helpful in directing clinical management of patients with microbial keratitis

Regulierung toxigener Pflanzenpathogene - Ökologische Dienstleistungen der Bodenfauna

Bodentiere bieten eine Vielfalt an ökologischen Funktionen und Dienstleistungen. Dazu zählen beispielsweise die Stimulierung von Zersetzungsprozessen und der Abbau von Pflanzenrückständen, was eine Regulierung von Pflanzenpathogenen und eine Reduzierung von Schadstoffen zur Folge haben kann. Ungeklärt blieb bislang allerdings, inwiefern bestimmte Schlüsselorganismen (Regenwürmer: Lumbricus terrestris; Collembolen: Folsomia candida und Nematoden: Aphelenchoides saprophilus) und deren Interaktion einen signifikanten Beitrag zum Abbau von phytopathogenen und toxigenen Schadpilzen der Gattung Fusarium in Ernterückständen von Getreide leisten und somit zur Lösung agrarrelevanter Umweltprobleme beitragen könnten. Aus diesem Grund wurden 2011 und 2013 Untersuchungen im Freiland durchgeführt, anhand derer folgende Hypothesen geprüft werden sollten: (1) Die eingesetzten Bodentiere fördern den Abbau von Fusarium-Biomasse im Stroh; (2) Die Interaktion zwischen den Vertretern der Makrofauna, Mesofauna und Mikrofauna verstärkt die Reduzierung der Fusarium-Biomasse. In beiden Versuchsjahren wurde nach Versuchsende (4 und 8 Wochen) in Stroh und Boden die Fusarium-Biomasse in Form von Fusarium-Protein-Äquivalenten (FPE) mittels der ELISA (Enzyme-linked immunosorbent assay)-Methodik quantitativ bestimmt. Außerdem wurde die Biomasse der Regenwürmer sowie die Individuenzahlen von Collembolen und Nematoden erfasst. Als Parameter für die Attraktivität des Strohs wurde das Verhältnis zwischen den Anteilen des verbliebenen Strohs auf der Bodenoberfläche und des von den Regenwürmern in den Boden eingearbeiteten Strohs bestimmt. Die eingesetzten Bodentiere förderten den Abbau von Fusarium-Biomasse in Weizenstroh, wobei L. terrestris in seiner Funktion als Primärzersetzer den wesentlichen Beitrag leistete. Der Einfluss von Collembolen und Nematoden stellte sich in Anwesenheit von L. terrestris als vernachlässigbar heraus, da die Interaktion nicht zu einem verstärkten Rückgang der Fusarium-Biomasse führte. Im Wirkungsgeflecht zwischen Bodentieren und pilzlichen Schaderregern tragen vor allem anektische, detritivore Regenwürmer durch aktive Regulierung von Pflanzenpathogenen maßgeblich zur Erhaltung der Bodengesundheit als ökologische Dienstleistung in Agrarökosystemen bei

Adaptive servoventilation improves cardiac function and respiratory stability

Cheyne–Stokes respiration (CSR) in patients with chronic heart failure (CHF) is of major prognostic impact and expresses respiratory instability. Other parameters are daytime pCO2, VE/VCO2-slope during exercise, exertional oscillatory ventilation (EOV), and increased sensitivity of central CO2 receptors. Adaptive servoventilation (ASV) was introduced to specifically treat CSR in CHF. Aim of this study was to investigate ASV effects on CSR, cardiac function, and respiratory stability. A total of 105 patients with CHF (NYHA ≥ II, left ventricular ejection fraction (EF) ≤ 40%) and CSR (apnoea–hypopnoea index ≥ 15/h) met inclusion criteria. According to adherence to ASV treatment (follow-up of 6.7 ± 3.2 months) this group was divided into controls (rejection of ASV treatment or usage <50% of nights possible and/or <4 h/night; n = 59) and ASV (n = 56) adhered patients. In the ASV group, ventilator therapy was able to effectively treat CSR. In contrast to controls, NYHA class, EF, oxygen uptake, 6-min walking distance, and NT-proBNP improved significantly. Moreover, exclusively in these patients pCO2, VE/VCO2-slope during exercise, EOV, and central CO2 receptor sensitivity improved. In CHF patients with CSR, ASV might be able to improve parameters of SDB, cardiac function, and respiratory stability

Spectroscopic studies of individual plasmon resonant nanoparticles

We present a detailed description of the apparatus and techniques that we have utilized in our experimental study of individual plasmon resonant nanoparticles, along with a brief description of some major results. The apparatus consists of a spectroscopic system combined with a modified darkfield microscope, which enables the user to sequentially select individual resonant nanostructures in the microscopic field of view for spectroscopic study. Plasmon resonant nanostructures scatter light elastically, and typically have very large scattering cross-sections at their resonant optical wavelengths. In general, spectra can be obtained with acquisition times between .1 to 30 seconds, and color images can be captured using consumer digital color cameras. Spheres, tetrahedrons, and pentagonal platelets were fabricated using colloidal chemistry techniques. To produce highly anisotropic structures such as nanorods and "barbells", templates were used. Many of these nanostructures have been individually spectroscopically characterized, and their spectra correlated with their shape and size as determined by transmission electron microscope (TEM). The unique shape, size, composition, and dielectric surroundings of the individual plasmon resonant nanostructures determine their plasmon resonant behavior. We will show how the composition of the substrate on which the particles are immobilized and the dielectric of the surrounding medium have a significant effect on the plasmon resonance of the individual particles

Structural and functional characterizations of mung bean mitochondrial nucleoids

Mitochondrial nucleoids isolated from mung bean seedlings exhibited a chromatin-like structure associated with a membrane component. A similar structure, which underwent discrete changes during cotyledon development, was identified in situ. Isolated nucleoids consisted of essentially the same phospholipids, including cardiolipin, as whole mitochondria and proteins of inner- and outer-mitochondrial-membrane origin. Actin was consistently found with mitochondrial nucleoids prepared with different detergent concentrations. Formaldehyde cross-linking of cytochalasin B- and proteinase K-treated mitochondria further revealed that actin was associated with DNA in nucleoids. Mitochondrial nucleoids were self-sufficient in directing DNA synthesis in vitro in a pattern mimicking mtDNA synthesis in isolated mitochondria. In pulse-field gel electrophoresis, newly synthesized mtDNA separated into two major components, well-bound and fast-moving forms. Nucleoids DNA synthesis was resistant to aphidicolin but sensitive to N-ethylmaleimide, which indicates that a γ-type DNA polymerase was responsible for this activity. Mitochondrial nucleoids were capable of self-directed RNA transcription in a non-random fashion in vitro. Consistent with and complementary to results from fungi and human cells done mostly in situ, our present work helps to establish the important paradigm that mitochondrial nucleoids in eukaryotes are more than mere mtDNA compaction and segregation entities but are centers of mtDNA maintenance and expression