Effects of cultivar and agrobotanical storage treatment on organolepetic quality of yam (Dioscorea rotundata)

There is current interest in the search and use of agrobotanicals in preference to conventional chemicals in agriculture as plant protectants hence the need to investigate the potential effects of some of the agrobotanicals on yam food after storage. This study was, therefore, conducted to assess the effects of yam cultivar and agrobotanical extracts on the organoleptic quality and acceptability of two yam based food forms: boiled yam and pounded yam. Tubers of four yam cultivars (Nwaopoko,Danacha, Ezakwukpolo and Pepa) were treated with aqueous leaf extracts of Neem (Azadiracta indica), Scent leaf (Occimum gratissimum), pod extracts of ‘Uda’ (Xylopia aethiopica), root extracts of Ginger (Zingibger officinale) and a synthetic phytohomorne (Gibberellic acid) and stored for six months. Thereafter, two food forms (boiled yam and pounded yam) were prepared from the stored yam tubers and a nine-member panel of judges enlisted to perform sensory quality assessment on them. The attributes scored with respect to boiled yam were colour, taste, texture, mealiness and general acceptability while pounded yam was assessed for its aroma, texture, colour and general acceptability. No significant differences were found among the sensory attributes due to the agrobotanical treatments rather, differences were due to yam cultivars. The results obtained and confirmed by the scatter plots and multiple linear regression showed that texture and taste contributed much to the general acceptability of boiled yam while aroma and consistency were the major qualities of pounded yam. Consistency of pounded yam was best with Nwaopoko and Danacha cultivars but significantly less so with other cultivars with or without agrobotanical treatment. The agrobotanical treatments did not affect the culinary quality of the stored yam tubers as no evidence of such treatment was noticed in the prepared yam foods. This result suggests that the constituent active ingredients imparted on the tubers during storage by the plant extracts were either water-soluble and heat labile or that their shelf lives were less than six months of the storage period and therefore had no effects on the quality attributes of food yams

A Comparative Assessment of the Fatty Acid and Phospholipid Composition of Irvingia Gabonensis (African Wild Mango) and Citrullus Lanatus (Water Melon) Seed Oils

The fatty acid composition of Irvingia gabonensis (African wild mango) and Citrullus lanatus (water melon) seed oils were determined using gas chromatographic technique with flame ionization detector (GC-FID). The results indicated eight fatty acids in Citrullus lanatus and eleven in Irvingia gabonensis seed oils. Comparatively, fatty acids common to both seed oils are linoleic acid (61.07%, 22.98%), oleic acid (13.48%, 21.87%), palmitic acid (15.40%, 16.69%), palmitoleic acid (0.53%, 6.32%), linolenic acid   (0.52% , 17.55%) and erucic acid (0.54%,0.88%). Lauric acid, arachidic acid, myristic acid and behenic acid though found in Irvingia gabonensis were not traceable in citrullus lanatus while arachidonic acid was present in citrullus lanatus but not in irvingia gabonensis. Margaric acid and lignoceric acid were not found in both seed oils. Irvingia gabonensis seed oil presented potential for making hard soaps. The seed oils generally presented potential for industrial use. Phospholipid levels in Citrullus Lanatus and Irvingia gabonensis seed oils on the other hand was also determined using gas chromatographic technique with pulse flame photometric detector (GC-PFPD). The result showed six phospholipids in each seed oil namely phosphatidylcholine, phosphatidylinositol, phosphatidylethanolamine, phosphatidylserine, lysophosphatidylcholine and phosphatidic acid. The total phospholipid levels in Citrullus lanatus seed oil is higher (935.65 mg/100g) than that in Irvingia gabonensis (239.89 mg/100g) seed oils. Phosphatidylcholine was the most abundant in both seed oils while phosphatidylserine was the least concentrated. Citrullus lanatus seed oils present a higher potential for health benefits based on their phospholipid levels than Irvingia gabonensis. The study indicates that Irvingia gabonensis and citrullus lanatus would be useful in soap making industries and for improved health benefits respectively. Keywords: African wild mango, Citrullus lanatus, Chromatography, fatty acids, Irvingia gabonensis, industrial potential, Phospholipids, Phosphatidic acid, seed oil

Incorporating development of a patient-reported outcome instrument in a clinical drug development program: examples from a heart failure program.

BackgroundPatient-reported outcome (PRO) measures can be used to support label claims if they adhere to US Food & Drug Administration guidance. The process of developing a new PRO measure is expensive and time-consuming. We report the results of qualitative studies to develop new PRO measures for use in clinical trials of omecamtiv mecarbil (a selective, small molecule activator of cardiac myosin) for patients with heart failure (HF), as well as the lessons learned from the development process.MethodsConcept elicitation focus groups and individual interviews were conducted with patients with HF to identify concepts for the instrument. Cognitive interviews with HF patients were used to confirm that no essential concepts were missing and to assess patient comprehension of the instrument and items.ResultsDuring concept elicitation, the most frequently reported HF symptoms were shortness of breath, tiredness, fluid retention, fatigue, dizziness/light-headedness, swelling, weight fluctuation, and trouble sleeping. Two measures were developed based on the concepts: the Heart Failure Symptom Diary (HF-SD) and the Heart Failure Impact Scale (HFIS). Findings from cognitive interviews suggested that the items in the HF-SD and HFIS were relevant and well understood by patients. Multiple iterations of concept elicitation and cognitive interviews were needed based on FDA request for a broader patient population in the qualitative study. Lessons learned from the omecamtiv mecarbil PRO/clinical development program are discussed, including challenges of qualitative studies, patient recruitment, expected and actual timelines, cost, and engagement with various stakeholders.ConclusionDevelopment of a new PRO measure to support a label claim requires significant investment and early planning, as demonstrated by the omecamtiv mecarbil program

Evaluation of Toxicological Effects of Spondias Mombin in Adult Male Wistar Rats

Spondias mombin is relied on for various herbal remedies for numerous conditions. This study is to evaluate the acute toxic effect of mombin in adult male Wistar rats. Acute toxicity test was carried out with modified Lorke’s method. Twenty-five male rats weighing between 120-180g were used for the sub-chronic study. The rats were divided into five groups A- E (n=5). Group A served as control. Group B and C received respectively 250mgkg-1 and 500mgkg-1 body weight doses of aqueous leaf extract,  groups D and E received  250mgkg-1 and 500mgkg-1 body weight doses of ethanolic extract. Treatments lasted for twenty eight days. Acute toxicity test carried out showed that leaf extracts of Spondias mombin did not produce mortality in rats.  Significant (p<0.05) reduction in brain and kidney weights was observed in group E treated with 500mgkg-1 of ethanolic extract. Similarly, significant (p<0.05) reduction was observed in spleen weights in groups C and E that were treated with 500mgkg-1 of both extracts. The levels of LDL (p<0.001) and ALP (p<0.05) were significantly reduced.  Alterations in the histology of the liver and kidney of extracts treated groups were observed. Conclusion: This result suggests that although the use of the leaf extracts of Spondias mombin is relatively safe, hepatic and renal toxicity may occur with prolonged use. Keywords: Adverse effects, Mortality, Methods, Complication, Utilizatio

Co-composting of faecal sludge with solid waste to improve FSM practice in Sakhipur municipality

Sakhipur municipality with 32,000 inhabitants mostly comprise of onsite septic tank and pit toilet of different types. When the pit or septic tank is full, the usual practice is to engage professional sweepers are employed to manually empty them and deposit the wastes in mostly the nearby water bodies. A co-compost plant was designed by the Sakhipur municipality and partners and it’s been in operation since 2016 to tackle faecal sludge generated from pit toilets and septic tanks, and solid waste from households exploring sanitation service chain. This study provides a description of an innovative co-composting system which improves current FSM practice in municipality regime. The generated lessons against tackling the operational challenges of sanitation service chain will provide good learnings for replicating similar practice in other municipalities

The Testicular and Epididymal Expression Profile of PLCζ in Mouse and Human Does Not Support Its Role as a Sperm-Borne Oocyte Activating Factor

Phospholipase C zeta (PLCζ) is a candidate sperm-borne oocyte activating factor (SOAF) which has recently received attention as a potential biomarker of human male infertility. However, important SOAF attributes of PLCζ, including its developmental expression in mammalian spermiogenesis, its compartmentalization in sperm head perinuclear theca (PT) and its release into the ooplasm during fertilization have not been established and are addressed in this investigation. Different detergent extractions of sperm and head/tail fractions were compared for the presence of PLCζ by immunoblotting. In both human and mouse, the active isoform of PLCζ was detected in sperm fractions other than PT, where SOAF is expected to reside. Developmentally, PLCζ was incorporated as part of the acrosome during the Golgi phase of human and mouse spermiogenesis while diminishing gradually in the acrosome of elongated spermatids. Immunofluorescence localized PLCζ over the surface of the postacrosomal region of mouse and bull and head region of human spermatozoa leading us to examine its secretion in the epididymis. While previously thought to have strictly a testicular expression, PLCζ was found to be expressed and secreted by the epididymal epithelial cells explaining its presence on the sperm head surface. In vitro fertilization (IVF) revealed that PLCζ is no longer detectable after the acrosome reaction occurs on the surface of the zona pellucida and thus is not incorporated into the oocyte cytoplasm for activation. In summary, we show for the first time that PLCζ is compartmentalized as part of the acrosome early in human and mouse spermiogenesis and is secreted during sperm maturation in the epididymis. Most importantly, no evidence was found that PLCζ is incorporated into the detergent-resistant perinuclear theca fraction where SOAF resides

Hilbert space built over connections with a non-compact structure group

Quantization of general relativity in terms of SL(2,C)-connections (i.e. in terms of the complex Ashtekar variables) is technically difficult because of the non-compactness of SL(2,C). The difficulties concern the construction of a diffeomorphism invariant Hilbert space structure on the space of cylindrical functions of the connections. We present here a 'toy' model of such a Hilbert space built over connections whose structure group is the group of real numbers. We show that in the case of any Hilbert space built analogously over connections with any non-compact structure group (this includes some models presented in the literature) there exists an obstacle which does not allow to define a *-representation of cylindrical functions on the Hilbert space by the multiplication map which is the only known way to define a diffeomorphism invariant representation of the functions.Comment: 45 pages, no figures, LaTeX2e, the discussion of results extende

Causal attribution of mental illness in south-eastern Nigeria

Background: Understanding of mental illness in sub-Saharan Africa has remained under-researched in spite of the high and increasing neuropsychiatric burden of disease in the region. Aims: This study investigated the causal beliefs that the Igbo people of south-eastern Nigeria hold about schizophrenia, with a view to establishing the extent to which the population makes psychosocial, biological and supernatural attributions. Method: Multi-stage sampling was used to select participants (N = 200) to which questionnaires were administered. Results: Mean comparison of the three causal models revealed a significant endorsement of supernatural causation. Logistic regressions revealed significant contributions of old age and female gender to supernatural attribution; old age, high education and Catholic religious denomination to psychosocial attributions; and high education to biological attributions. Conclusions: It is hoped that the findings would enlighten, augment literature and enhance mental health care service delivery

Detection of Group 1 Coronaviruses in Bats in North America

Bats of 2 species harbor group 1 coronaviruses