737 research outputs found
IgE-mediated histamine release from nasal mucosa is inhibited by SLPI (secretory leukocyte protease inhibitor) to the level of spontaneous release.
The secretory leukocyte protease inhibitor (SLPI) is a low-molecular-weight inhibitor of proteases, such as elastase and cathepsin G which are released from leukocytes during phagocytosis. The purpose of this study was to determine whether or not SLPI is able to inhibit IgE-mediated histamine release. Nasal mucosa from 11 test subjects without atopic disposition was used for this in vitro study. We found that SLPI inhibited histamine release in a dose-dependent way but was without influence on the spontaneous release
Secretory leukocyte protease inhibitor in punch biopsies from human colonic mucosa.
Secretory leukocyte protease inhibitor (SLPI) is a well-known protease inhibitor. Its function is thought to be protease/protease-inhibitor balance. Free proteolytic activity, mainly pancreatic elastase, anionic trypsin and granulocytic elastase, has been demonstrated in faecal extracts from patients with ulcerative colitis. We wanted to verify that SLPI is actually secreted from normal human colonic mucosa. Also, we wanted to ascertain whether studies of SLPI secretion based on punch biopsies were dependent on biopsy area or on biopsy circumference. Normal colonic mucosa was sampled during surgery for colonic cancer. A total of 36 samples from four patients were used. Mucosa preparation was carried out using a punch biopsy technique, and samples of 3, 4 and 6 mm diameter were used. All media contained SLPI at varying concentrations. When expressed in terms of the sample area, the secretion per millimetre-squared seemed to decrease with increasing area. When calculated as secretion per circumference, secretion seemed to be constant. In conclusion, SLPI was secreted from normal human colonic mucosa. The SLPI secretion seemed dependent on the circumference of the biopsy rather than on the area of the biopsy
Coherent transient data-rate conversion and data transformation
Temporal compressions of optical pulses and pulse trains have been performed by the photon-echo process in Tm-doped YAG at 793 nm. Single-pulse temporal compression by almost a. factor of 500 from 10 mu s to 22 ns and pulse train compression by a factor of 14 from 5 mu s to 350 ns with a high-speed frequency-tunable external-cavity diode laser are demonstrated. It is suspected that significantly higher compression could be obtained by improved control of the laser frequency and laser frequency chirps. Theoretically, Tm-doped YAG should be capable of compressing single pulses by almost a factor of 10(7)
The presence of elafin, SLPI, IL1-RA and STNFalpha RI in head and neck squamous cell carcinomas and their relation to the degree of tumour differentiation.
Biopsy samples of head and neck carcinomas were investigated with regard to elafin, secretory leukocyte protease inhibitor (SLPI), interleukin 1-receptor antagonist [(IL)1-RA] and soluble tumour necrosis factor alpha receptor antagonist (STNFalpha RI). SLPI and elafin are serine protease inhibitors produced in the serous cells of the upper respiratory airways and in the keratinocytes, respectively. We have now found the presence of elafin and SLPI in squamous cell carcinomas of the upper respiratory tract (tonsillar, hypopharyngeal, tongue, mouth floor, gingival and laryngeal cancer). Significantly higher amounts of SLPI and elafin are present in well-differentiated and moderately differentiated tumours than in poorly differentiated tumours (p < 0.0001 and p < 0.0015). Tumour necrosis factor-alpha and IL-1beta have been shown to stimulate the production of SLPI and elafin. Since these cytokines can both be difficult to detect, we chose to study their inhibitors, STNFalpha RI and IL1-RA, instead. IL1-RA was expressed in highly differentiated tumours as well as in poorly differentiated ones. No significant difference was seen between the groups. STNFalpha RI was only found in very small amounts, sparsely distributed in the tumours, and was not related to the degree of differentiation
Levels of Free Granulocyte Elastase in Bronchial Secretions from Patients with Cystic Fibrosis: Effect of Antimicrobial Treatment Against Pseudomonas aeruginosa
Large amounts of free granulocyte elastase (GE), an enzyme capable of mediating airway damage, have been found in bronchial secretions of patients with cystic fibrosis who are infected with Pseudomonas aeruginosa. This finding indicates an imbalance between GE and its antiproteases, α1-proteinase inhibitor (α1-PI) and bronchial mucosal inhibitor (BMI), in the airways of these individuals. The effect of intravenous antimicrobial treatment against P. aeruginosa on activity and concentration of GE, BMI, and α1-PI was evaluated in 30 treatment courses of 20 patients with cystic fibrosis. Although sputum volume and level of immunoreactive GE decreased and concentrations of α1-PI and BMI increased significantly (P < .05), a high level of free GE persisted. No active α1-PI and BMI were detectable after treatment. High levels of GE correlated with a poor pulmonary condition (rs = .98, P < .001). In vitro, elastolytic activity of bronchial secretions from patients with cystic fibrosis was significantly inhibited by eglin C and an oxidation-resistant variant of α1-PI, both compounds currently produced by recombinant DNA technolog
The presence of elafin, SLPI, IL1-RA and STNFα RI in head and neck squamous cell carcinomas and their relation to the degree of tumour differentiation
Investigations of Optical Coherence Properties in an Erbium-doped Silicate Fiber for Quantum State Storage
We studied optical coherence properties of the 1.53 m telecommunication
transition in an Er-doped silicate optical fiber through spectral
holeburning and photon echoes. We find decoherence times of up to 3.8 s at
a magnetic field of 2.2 Tesla and a temperature of 150 mK. A strong
magnetic-field dependent optical dephasing was observed and is believed to
arise from an interaction between the electronic Er spin and the
magnetic moment of tunneling modes in the glass. Furthermore, we observed
fine-structure in the Erbium holeburning spectrum originating from
superhyperfine interaction with Al host nuclei. Our results show that
Er-doped silicate fibers are promising material candidates for quantum
state storage
Simple models of the chemical field around swimming plankton
Background. Cervical cancer is the fourth most common cancer in women, and we recently reported human leukocyte antigen (HLA) alleles showing strong associations with cervical neoplasia risk and protection. HLA ligands are recognized by killer immunoglobulin-like receptors (KIRs) expressed on a range of immune cell subsets, governing their proinflammatory activity. We hypothesized that the inheritance of particular HLA-KIR combinations would increase cervical neoplasia risk. Methods. Here, we used HLA and KIR dosages imputed from single-nucleotide polymorphism genotype data from 2143 cervical neoplasia cases and 13 858 healthy controls of European decent. Results. The following 4 novel HLA alleles were identified in association with cervical neoplasia, owing to their linkage disequilibrium with known cervical neoplasia-associated HLA-DRB1 alleles: HLA-DRB3*9901 (odds ratio [OR], 1.24; P = 2.49 × 10−9), HLA-DRB5*0101 (OR, 1.29; P = 2.26 × 10−8), HLA-DRB5*9901 (OR, 0.77; P = 1.90 × 10−9), and HLA-DRB3*0301 (OR, 0.63; P = 4.06 × 10−5). We also found that homozygosity of HLA-C1 group alleles is a protective factor for human papillomavirus type 16 (HPV16)-related cervical neoplasia (C1/C1; OR, 0.79; P = .005). This protective association was restricted to carriers of either KIR2DL2 (OR, 0.67; P = .00045) or KIR2DS2 (OR, 0.69; P = .0006). Conclusions. Our findings suggest that HLA-C1 group alleles play a role in protecting against HPV16-related cervical neoplasia, mainly through a KIR-mediated mechanism
A solid state light-matter interface at the single photon level
Coherent and reversible mapping of quantum information between light and
matter is an important experimental challenge in quantum information science.
In particular, it is a decisive milestone for the implementation of quantum
networks and quantum repeaters. So far, quantum interfaces between light and
atoms have been demonstrated with atomic gases, and with single trapped atoms
in cavities. Here we demonstrate the coherent and reversible mapping of a light
field with less than one photon per pulse onto an ensemble of 10 millions atoms
naturally trapped in a solid. This is achieved by coherently absorbing the
light field in a suitably prepared solid state atomic medium. The state of the
light is mapped onto collective atomic excitations on an optical transition and
stored for a pre-programmed time up of to 1 mu s before being released in a
well defined spatio-temporal mode as a result of a collective interference. The
coherence of the process is verified by performing an interference experiment
with two stored weak pulses with a variable phase relation. Visibilities of
more than 95% are obtained, which demonstrates the high coherence of the
mapping process at the single photon level. In addition, we show experimentally
that our interface allows one to store and retrieve light fields in multiple
temporal modes. Our results represent the first observation of collective
enhancement at the single photon level in a solid and open the way to multimode
solid state quantum memories as a promising alternative to atomic gases.Comment: 5 pages, 5 figures, version submitted on June 27 200
Searching for sterile neutrinos in ice
Oscillation interpretation of the results from the LSND, MiniBooNE and some
other experiments requires existence of sterile neutrino with mass eV
and mixing with the active neutrinos . It has
been realized some time ago that existence of such a neutrino affects
significantly the fluxes of atmospheric neutrinos in the TeV range which can be
tested by the IceCube Neutrino Observatory. In view of the first IceCube data
release we have revisited the oscillations of high energy atmospheric neutrinos
in the presence of one sterile neutrino. Properties of the oscillation
probabilities are studied in details for various mixing schemes both
analytically and numerically. The energy spectra and angular distributions of
the events have been computed for the simplest mass, and
mixing schemes and confronted with the IceCube data. An
illustrative statistical analysis of the present data shows that in the
mass mixing case the sterile neutrinos with parameters required by
LSND/MiniBooNE can be excluded at about level. The
mixing scheme, however, can not be ruled out with currently available IceCube
data.Comment: 41 pages, 16 figures. Accepted for publication in JHEP. Minor changes
from the previous versio
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