1,670 research outputs found
Cramer-Rao Lower Bound for Point Based Image Registration with Heteroscedastic Error Model for Application in Single Molecule Microscopy
The Cramer-Rao lower bound for the estimation of the affine transformation
parameters in a multivariate heteroscedastic errors-in-variables model is
derived. The model is suitable for feature-based image registration in which
both sets of control points are localized with errors whose covariance matrices
vary from point to point. With focus given to the registration of fluorescence
microscopy images, the Cramer-Rao lower bound for the estimation of a feature's
position (e.g. of a single molecule) in a registered image is also derived. In
the particular case where all covariance matrices for the localization errors
are scalar multiples of a common positive definite matrix (e.g. the identity
matrix), as can be assumed in fluorescence microscopy, then simplified
expressions for the Cramer-Rao lower bound are given. Under certain simplifying
assumptions these expressions are shown to match asymptotic distributions for a
previously presented set of estimators. Theoretical results are verified with
simulations and experimental data
Criterion for purely elastic Taylor-Couette instability in the flows of shear-banding fluids
In the past twenty years, shear-banding flows have been probed by various
techniques, such as rheometry, velocimetry and flow birefringence. In micellar
solutions, many of the data collected exhibit unexplained spatio-temporal
fluctuations. Recently, it has been suggested that those fluctuations originate
from a purely elastic instability of the flow. In cylindrical Couette geometry,
the instability is reminiscent of the Taylor-like instability observed in
viscoelastic polymer solutions. In this letter, we describe how the criterion
for purely elastic Taylor-Couette instability should be adapted to
shear-banding flows. We derive three categories of shear-banding flows with
curved streamlines, depending on their stability.Comment: 6 pages, 3 figure
Potential "ways of thinking" about the shear-banding phenomenon
Shear-banding is a curious but ubiquitous phenomenon occurring in soft
matter. The phenomenological similarities between the shear-banding transition
and phase transitions has pushed some researchers to adopt a 'thermodynamical'
approach, in opposition to the more classical 'mechanical' approach to fluid
flows. In this heuristic review, we describe why the apparent dichotomy between
those approaches has slowly faded away over the years. To support our
discussion, we give an overview of different interpretations of a single
equation, the diffusive Johnson-Segalman (dJS) equation, in the context of
shear-banding. We restrict ourselves to dJS, but we show that the equation can
be written in various equivalent forms usually associated with opposite
approaches. We first review briefly the origin of the dJS model and its initial
rheological interpretation in the context of shear-banding. Then we describe
the analogy between dJS and reaction-diffusion equations. In the case of
anisotropic diffusion, we show how the dJS governing equations for steady shear
flow are analogous to the equations of the dynamics of a particle in a quartic
potential. Going beyond the existing literature, we then draw on the Lagrangian
formalism to describe how the boundary conditions can have a key impact on the
banding state. Finally, we reinterpret the dJS equation again and we show that
a rigorous effective free energy can be constructed, in the spirit of early
thermodynamic interpretations or in terms of more recent approaches exploiting
the language of irreversible thermodynamics.Comment: 14 pages, 6 figures, tutorial revie
Mechanism of Vanadium Leaching during Surface Weathering of Basic Oxygen Furnace Steel Slag Blocks: A Microfocus X-ray Absorption Spectroscopy and Electron Microscopy Study
© 2017 American Chemical Society. Basic oxygen furnace (BOF) steelmaking slag is enriched in potentially toxic V which may become mobilized in high pH leachate during weathering. BOF slag was weathered under aerated and air-excluded conditions for 6 months prior to SEM/EDS and μXANES analysis to determine V host phases and speciation in both primary and secondary phases. Leached blocks show development of an altered region in which free lime and dicalcium silicate phases were absent and Ca-Si-H was precipitated (CaCO 3 was also present under aerated conditions). μXANES analyses show that V was released to solution as V(V) during dicalcium silicate dissolution and some V was incorporated into neo-formed Ca-Si-H. Higher V concentrations were observed in leachate under aerated conditions than in the air-excluded leaching experiment. Aqueous V concentrations were controlled by Ca 3 (VO 4 ) 2 solubility, which demonstrate an inverse relationship between Ca and V concentrations. Under air-excluded conditions Ca concentrations were controlled by dicalcium silicate dissolution and Ca-Si-H precipitation, leading to relatively high Ca and correspondingly low V concentrations. Formation of CaCO 3 under aerated conditions provided a sink for aqueous Ca, allowing higher V concentrations limited by kinetic dissolution rates of dicalcium silicate. Thus, V release may be slowed by the precipitation of secondary phases in the altered region, improving the prospects for slag reuse
Correlation length of hydrophobic polyelectrolyte solutions
The combination of two techniques (Small Angle X-ray Scattering and Atomic
Force Microscopy) has allowed us to measure in reciprocal and real space the
correlation length of salt-free aqueous solutions of highly charged
hydrophobic polyelectrolyte as a function of the polymer concentration ,
charge fraction and chain length . Contrary to the classical behaviour
of hydrophilic polyelectrolytes in the strong coupling limit, is strongly
dependent on . In particular a continuous transition has been observed from
to when decreased from 100% to
35%. We interpret this unusual behaviour as the consequence of the two features
characterising the hydrophobic polyelectrolytes: the pearl necklace
conformation of the chains and the anomalously strong reduction of the
effective charge fraction.Comment: 7 pages, 5 figures, submitted to Europhysics Letter
Temporal evolution of the transpolar potential after a sharp enhancement in solar wind dynamic pressure
Peer Reviewedhttp://deepblue.lib.umich.edu/bitstream/2027.42/95384/1/grl23891.pd
The Chicken Yolk Sac IgY Receptor, a Mammalian Mannose Receptor Family Member, Transcytoses IgY across Polarized Epithelial Cells
In mammals the transfer of passive immunity from mother to young is mediated by the MHC-related receptor FcRn, which transports maternal IgG across epithelial cell barriers. In birds, maternal IgY in egg yolk is transferred across the yolk sac to passively immunize chicks during gestation and early independent life. The chicken yolk sac IgY receptor (FcRY) is the ortholog of the mammalian phospholipase A2 receptor, a mannose receptor family member, rather than an FcRn or MHC homolog. FcRn and FcRY both exhibit ligand binding at the acidic pH of endosomes and ligand release at the slightly basic pH of blood. Here we show that FcRY expressed in polarized mammalian epithelial cells functioned in endocytosis, bidirectional transcytosis, and recycling of chicken FcY/IgY. Confocal immunofluorescence studies demonstrated that IgY binding and endocytosis occurred at acidic but not basic pH, mimicking pH-dependent uptake of IgG by FcRn. Colocalization studies showed FcRY-mediated internalization via clathrin-coated pits and transport involving early and recycling endosomes. Disruption of microtubules partially inhibited apical-to-basolateral and basolateral-to-apical transcytosis, but not recycling, suggesting the use of different trafficking machinery. Our results represent the first cell biological evidence of functional equivalence between FcRY and FcRn and provide an intriguing example of how evolution can give rise to systems in which similar biological requirements in different species are satisfied utilizing distinct protein folds
Towards a Linked democracy model
In this chapter we lay out the properties of participatory ecosystems as linked democracy ecosystems. The goal is to provide a conceptual roadmap that helps us to ground the theoretical foundations for a meso-level, institutional theory of democracy. The identification of the basic properties of a linked democracy eco-system draws from different empirical examples that, to some extent, exhibit some of these properties. We then correlate these properties with Ostrom's design principles for the management of common-pool resources (as generalised to groups cooperating and coordinating to achieve shared goals) to open up the question of how linked democracy ecosystems can be governed
Binary separation in very thin nematic films: thickness and phase coexistence
The behavior as a function of temperature of very thin films (10 to 200 nm)
of pentylcyanobiphenyl (5CB) on silicon substrates is reported. In the vicinity
of the nematic/isotropic transition we observe a coexistence of two regions of
different thicknesses: thick regions are in the nematic state while thin ones
are in the isotropic state. Moreover, the transition temperature is shifted
downward following a 1/h^2 law (h is the film thickness). Microscope
observations and small angle X-ray scattering allowed us to draw a phase
diagram which is explained in terms of a binary first order phase transition
where thickness plays the role of an order parameter.Comment: 5 pages, 3 figures, submitted to PRL on the 26th of Apri
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