Quality of two table grape guard cultivars treated with single or dual-phase release SO2 generators

Berries of seeded table grapes (cultivars Napoleón and Aledo) were trimmed in commercial packing houses, inoculated with Botrytis cinerea, packed with single and dual-phase release SO2 pads, and stored for up to four months at 0 ºC and 85±5 % relative humidity. Control grapes with or without inoculation, but without SO2 pads, were stored for up to 2 months. Botrytis cinerea rot (grey mould) limited the shelf-life of Aledo grapes to one month, while a two month shelf-life was established for Napoleón grapes, which suffered from berry splitting, Cladosporium herbarum and Botrytis cinerea rots. Yeasts of the Candida genera and secondary fungi were also identified in fruit suffering sour rot after 2 months at 0oC. No differences in grey mould development in the treatments without SO2 pads with or without inoculation. The native grey mould of these grapes was purified and included as the B. cinerea strain 20248 in the Spanish Type Culture Collection. Storage time, but not SO2 pads, slightly affected fruit quality. During the first month at 0oC, total soluble solids decreased by 1oBrix in 'Áledo' and berry hardness temporarily increased by around 30% in both cultivars. The pads provoked an SO2 taste acceptable for consumption, which was slightly higher in Napoleón than in Aledo grapes. The dual-phase release SO2 pads showed better performance for the long-term storage of grapes than single-release pads (both as regards berry sensory attributes and stem appearance, with lower stem browning). The dual-phase release SO2 pads extended the shelf-life of grapes by around 1 month, depending on the cultivar. Napoleón grapes showed a better potential for longterm storage than Aledo grapes due to thicker and more compact epidermis, thicker cell walls, and different epidermal microstructure including the transition cells between epidermis and the parenchyma

Factores asociados a insuficiencia renal postoperatoria en cirugía de revascularización miocárdica

Motivation: To determine which medical history and surgical procedure factors are associated to the onset of postoperative kidney failure in patients undergoing myocardial revascularisation. Methods: Case-control cohort study carried out between January 2005 and December 2013 which included patients who had undergone elective myocardial revascularisation and showed postoperative kidney failure immediately after surgery and until discharge. Controls consisted of patients who had undergone elective myocardial revascularisation and did not develop postoperative kidney failure immediately after surgery. A logistic regression model was used to determine the factors associated to postoperative kidney failure. Associations were expressed as grounds of disparity with their corresponding confidence intervals. Results: old age [OR 1.03 CI 95% (1.01-1.04)], preoperative presence of diabetes mellitus [OR 1.8 CI 95% (1.9-3.4)], cardiac insufficiency [OR 2.7 CI 95% (1.1-6.7)] and a longer perfusion time [OR 1.02 CI 95% (1.01-1.03)] were associated to a higher risk of postoperative kidney failure, while higher hematocrit [OR 0.86 CI 95% (0.82-0.91)] and higher ejection fraction [OR 0.94 CI 95% (0.92-0.96)] were associated with a decrease of the risk of postoperative kidney failure. Conclusions: In patients who had undergone myocardial revascularisation, risk factors associated to postoperative kidney failure where comorbidities related to internal and external gradual kidney damage outside the context of the surgery. This implies that strategies to minimise this event should be focused on identifying these patients in a timely manner and offering appropriate nephroprotection. © 2015 Sociedad Colombiana de Cardiología y Cirugía Cardiovascular

Alimentación de cerdos con suero de queso fresco en combinación con subproductos de molinería o grano de maíz

En el primer ensayo se midieron los rendimientos zootécnicos de cerdos durante los períodos de crecimiento y acabado (17 a 90 kg) alimentados con 7 raciones: 2 sin suero de queso fresco (SQF) basadas en maíz más torta de soya (M más TS) y maíz más torta de algodón (M más TA) o dietas controles, y las otras 5 con SQF en combinación con maíz-grano molido (MGM), o uno de los 4 subproductos de molinería siguientes: harina de arroz (HA), mogolla de trigo (MT), salvado de trigo (ST) y salvado de maíz (SM). En el segundo ensayo se midió el efecto de la alimentación con SQF, únicamente durante el período de acabado (43 a 92 kg) en combinación con los mismos subproductos de molinería del primer ensayo. Para los ensayos se emplearon 84 cerdos, mitad hembras y mitad machos castrados, de las razas Duroc (D), Landrace (L) y Yorkshire (Y), y cruces D x Y L x D, los cuales se asignaron al azar a cada ración. Los resultados del primer ensayo fueron: aumento de peso diario (APD) para el grupo SQF más HA: 600 gr; para el grupo SQF más MT: 580 gr y para el grupo SQF más MGM: 620 gr, aumentos que fueron inferiores a los obtenidos con las dietas controles (M más TS y M más TA), pero superiores significativamente a los obtenidos por los grupos SQF más SM: 490 gr y SQF más ST: 520 gr. El consumo promedio diario (CPD) de SQF fue de 15.3 y 21 L para los períodos de crecimiento y acabado, respectivamente. El CPD de subproductos de molinería y/o el MGM para los períodos mencionados fueron 1.1 y 1.5 kg, respectivamente. Los resultados del segundo ensayo tuvieron las mismas respuestas que en el primer estudio, pero con un menor CPD de SQF igual a 17 L y un mayor CPD de subproducto de molinería o MGM igual a 2 kg, lo cual sugiere falta de adaptación de su capacidad gastrointestinal. Estos resultados permitieron concluir que el SQF puede reemplazar en un 33 por ciento al concentrado balanceado convencional y no debe ser combinado con subproductos cuyo contenido final de fibra sea igual o exceda al 10 por ciento, vale decir, como SM o ST.In the first study, animal performance was measured on growing and finishing pigs (17 to 90 kg) feed seven diets: two with out whey, corn-soy bean meal (CSM) and corn-cotton seed oil meal (CCM); and the other five, with whole fresh whey (WFW) in combination with ground corn grain (GC), or one of the following four grain milling by-products: Rice bran (RB), wheat middlings (WM), wheat bran (WB) and corn bran (CB). In a second trial the effects of feeding WFW was studied by feedings pigs in the finishing fase (43 to 92 kg), that is, pigs that had not been expossed to whey in their diets during the growth period; WFW in combination with the milling by products: RB, WM, WB and CB. Duroc (D), Landrace (L) and Yorkshire (Y), and Duroc cross bred pigs (D x V and L x D), were used in this study. Employing a total of 84 pigs, 12 (six castrates and six females) were randomly alloted to each diet. Results of the first trial were: average daily gain (ADG) for the WFW-RB troup, 600 g; WFW-WM group, 580 g and the WFW-GC group, 620 g. These ADG were inferior to those abtained with the pigs on the control diets CSM and CCM, but superior to those groups of pigs on the WFW-CB (490 g) and WFW-WB (520 g) diets (P< 0.05). WFW daily average intake during the growth period was 15.3 liters and during the finishing period, 21.0 liters; average daily grain milling by-product intake for the two periods were: 1.1 and 1.5 kg, respectively. Results of the second study for those animals that received no WFW during the growth period, showed the same results as those of the first study, but with a lower WFW daily intake (17 liters/day), and a higher grain milling by-product intake: 2.0 kg/day. This fact suggests an adaptation of the gastro-intestinal tract in terms of liquid volume capacity of pigs fed WFW during the growth period. It is concluded that WFW can replace 33% of a conventional balanced diet, and should not be combined in rations whose total crude fiber content is equal to or exceeds 10%, such that will readly accur when wheat bran and corn bran are used.Maíz-zea maysPorcicultur

Diseño y construcción de un prototipo controlado para carburización gaseosa de aceros utilizando alcoholes

En esta investigación se realizó el diseno, construcción y prueba de un equipo a escala de laboratorio para tratamientos térmicos con atmósfera controlada para el estudio de la carburización gaseosa de aceros con alcoholes. El equipo posee las siguientes caracterIsticas: producción controlada de una atmósfera gaseosa compuesta hasta por tres gases y dos lIquidos volatiles, una zona de reacción isotérmica, inerte y hermética, y una zona de analisis de gases que retiene sólidos y lIquidos condensables y que cuenta con sensores de presión y de dióxido de carbono. Se hicieron ocho ensayos con el alcohol metIlico, etIlico, n-propIlico, isopropIlico y tertbutanol, encontrandose que el alcohol metIlico carburiza y produce una bala cantidad de hollIn pero profundiza poco en la pieza, el etanol descarburiza y forma grandes cantidades de hollIn, el n-propanol, isopropanol y el tertbutanol proveen buenos potenciales de carbono y profundidades de capa con una gran formación de hollIn y alquitran, siendo en orden ascendente en producción de hollIn y alquitran el n-propanol, el tertbutanol y el isopropanol.This article deals with designing, constructing and testing a laboratory scale apparatus for heat treatment with controlled atmosphere. The equipment was used for studying steel carburisation with alcohols. The equipment has an area for the controlled production of a gas atmosphere composed by up to 3 gases and 2 volatile liquids, an inert and hermetic isothermal reaction area and a gas analysis area which retains solids and condensable liquids. The equipment was provided with pressure and carbon dioxide concentration sensors. Eight experiments were carried out with methanol, ethanol, n-propanol, isopropanol and tertbutanol as carbon source. The results showed that methanol caused carburisation but layer depth was small and produced a low quantity of soot, ethanol caused decarburisation and formed large quantities of soot, n-propanol, isopropanol and the tertbutanol provided good carbon potential and layers having good depth with good soot and tar production. Soot and tar production followed this order: n-propanol and lt; tertbutanol and lt; isopropanol

Antimicrobial Potential Of Some Plant Extracts Against Candida Species.

The increase in the resistance to antimicrobial drugs in use has attracted the attention of the scientific community, and medicinal plants have been extensively studied as alternative agents for the prevention of infections. The Candida genus yeast can become an opportunistic pathogen causing disease in immunosuppressive hosts. The purpose of this study was to evaluate dichloromethane and methanol extracts from Mentha piperita, Rosmarinus officinalis, Arrabidaea chica, Tabebuia avellanedae, Punica granatum and Syzygium cumini against Candida species through the analysis of Minimum Inhibitory Concentration (MIC). Results presented activity of these extracts against Candida species, especially the methanol extract.701065-

Monitoreo remoto a sistemas de riego por bombeo eléctrico a cultivos de arroz en el departamento del Huila

It was developed an integrated system that is responsible for monitoring the state of the main variables of an irrigation system (water flow, temperature and magnetic state of the pump motor) and send this information to the end user in real time via Internet through a website that was designed for this purpose. The system comprises three sensors (temperature, flow and magnetic state), which a smartcard development (Arduino) responsible for the acquisition and processing of the magnitude of the variable, two modules wireless transmission (XBee) connect which connect the electronic board to a computer (web server), which with an Internet connection is responsible for sending this information to the user or system administrator by email. The monitoring system is propose to Electrohuila SA ESP may offer it as a value-added service users to agricultural irrigation systems with electrical pumping. Electrohuila ESP SA is the service company responsible for distribution of electricity in the department of Huila, Colombia.Se ha desarrollado un sistema integral que se encarga de monitorear el estado de las principales variables de un sistema de riego (caudal de agua, temperatura y estado magnético del motor de bombeo) y enviar esta información en tiempo real al usuario final mediante Internet a través de una página web que se diseñó para este fin. El sistema está compuesto por tres sensores (temperatura, caudal y estado magnético), los cuales se conectan a una tarjeta electrónica de desarrollo (Arduino) encargada de la adquisición y procesamiento de la magnitud de las variables, dos módulos de transmisión inalámbrica (XBee) que intercomunican la tarjeta electrónica con un computador (servidor web), el cual con una conexión a Internet se encarga de enviar ésta información al usuario o administrador del sistema por E-mail. El sistema de monitoreo está planteado para que Electrohuila S.A E.S.P pueda ofrecerlo como servicio de valor agregado a usuarios agrícolas con sistemas de riego con bombeo eléctrico. Electrohuila S.A E.S.P es la empresa encargada del servicio de distribución de energía eléctrica en el departamento del Huila, Colombia

SARS-CoV-2 Seroprevalence Study in Pediatric Patients and Health Care Workers Using Multiplex Antibody Immunoassays

SARS-CoV-2 infection has become a global health problem specially exacerbated with the continuous appearance of new variants. Healthcare workers (HCW) have been one of the most affected sectors. Children have also been affected, and although infection generally presents as a mild disease, some have developed the Pediatric Inflammatory Multisystem Syndrome Temporally Associated with SARS-CoV-2 (PIMS-TS). We recruited 190 adults (HCW and cohabitants, April to June 2020) and 57 children (April 2020 to September 2021), of whom 12 developed PIMS-TS, in a hospital-based study in Spain. Using an in-house Luminex assay previously validated, antibody levels were measured against different spike and nucleocapsid SARS-CoV-2 proteins, including the receptor-binding domain (RBD) of the Alpha, Beta, Gamma, and Delta variants of concern (VoC). Seropositivity rates obtained from children and adults, respectively, were: 49.1% and 11% for IgG, 45.6% and 5.8% for IgA, and 35.1% and 7.3% for IgM. Higher antibody levels were detected in children who developed PIMS-TS compared to those who did not. Using the COVID-19 IgM/IgA ELISA (Vircell, S.L.) kit, widely implemented in Spanish hospitals, a high number of false positives and lower seroprevalences compared with the Luminex estimates were found, indicating a significantly lower specificity and sensitivity. Comparison of antibody levels against RBD-Wuhan versus RBD-VoCs indicated that the strongest positive correlations for all three isotypes were with RBD-Alpha, while the lowest correlations were with RBD-Delta for IgG, RBD-Gamma for IgM, and RBD-Beta for IgA. This study highlights the differences in antibody levels between groups with different demographic and clinical characteristics, as well as reporting the IgG, IgM, and IgA response to RBD VoC circulating at the study period