The Thinking of “Negative List” Management Mode Implemented by Administrative Approval System

“Negative list” management as a key point in the economic system reform will be of great reference to deepen the reform of administrative approval system which can be regarded as a sally port to promote the transformation of government functions. This paper tries to analyses feasibility of the mode to promote administrative system reform and its key points and difficulties in the operation process by drawing lessons from “negative list” management in free trade area and the development of foreign experience as well as combining the characteristics of “negative list” management mode.

Path Analysis of Construction of New Rural Communities in Zihe Town of Santai County

The 18th National Congress of Communist Party of China (CPC) put forward the integration of urban and rural development is the fundamental way to solve the “three rural” problems. In order to achieve this goal, We should increase urban and rural development efforts to promote the common prosperity of urban and rural areas. Zihe town in Santai county of Sichuan province takes the initiative to adapt to the development of urban and rural areas, so that the masses of farmers by the villagers into the residents, the real people of urbanization. On the basis of investigation and study, this paper expounds construction of new rural communities in Zihe town of Santai county in Sichuan province .the general situation and puts forward some policy suggestions

Path Analysis of Construction of New Rural Communities in Zihe Town of Santai County

The 18th National Congress of Communist Party of China (CPC) put forward the integration of urban and rural development is the fundamental way to solve the “three rural” problems. In order to achieve this goal, We should increase urban and rural development efforts to promote the common prosperity of urban and rural areas. Zihe town in Santai county of Sichuan province takes the initiative to adapt to the development of urban and rural areas, so that the masses of farmers by the villagers into the residents, the real people of urbanization. On the basis of investigation and study, this paper expounds construction of new rural communities in Zihe town of Santai county in Sichuan province .the general situation and puts forward some policy suggestions

An Analysis of the Problems in the Law Enforcement of Urban Management Department and Suggested Counter Measures

The success of building an urban management officer team to comprehensively manage the work in urban areas should only be achieved by taking into consideration the status quo of China’s urban management and by learning from international advanced experience. The complexity of China’s urban management as well as the slowness in formulating laws and regulations determine that the existence of urban management department and the way of its law enforcement should be in a progressive manner but not be achieved overnight. On the basis of conducting an objective analysis of the development of China’s urban management officer team, the paper draws on the advanced experience of urban management in foreign countries and absorb scientific management methods to explore the approaches to developing China’s future urban management department. Key words: Urban management department; Law enforcement of urban management departmen

Fabricating 3-dimensional human brown adipose microtissues for transplantation studies

Transplanting cell cultured brown adipocytes (BAs) represents a promising approach to prevent and treat obesity (OB) and its associated metabolic disorders, including type 2 diabetes mellitus (T2DM). However, transplanted BAs have a very low survival rate in vivo. The enzymatic dissociation during the harvest of fully differentiated BAs also loses significant cells. There is a critical need for novel methods that can avoid cell death during cell preparation, transplantation, and in vivo. Here, we reported that preparing BAs as injectable microtissues could overcome the problem. We found that 3D culture promoted BA differentiation and UCP-1 expression, and the optimal initial cell aggregate size was 100 μm. The microtissues could be produced at large scales via 3D suspension assisted with a PEG hydrogel and could be cryopreserved. Fabricated microtissues could survive in vivo for long term. They alleviated body weight and fat gain and improved glucose tolerance and insulin sensitivity in high-fat diet (HFD)-induced OB and T2DM mice. Transplanted microtissues impacted multiple organs, secreted protein factors, and influenced the secretion of endogenous adipokines. To our best knowledge, this is the first report on fabricating human BA microtissues and showing their safety and efficacy in T2DM mice. The proposal of transplanting fabricated BA microtissues, the microtissue fabrication method, and the demonstration of efficacy in T2DM mice are all new. Our results show that engineered 3D human BA microtissues have considerable advantages in product scalability, storage, purity, safety, dosage, survival, and efficacy

Anchoring active Pt2+/Pt0 hybrid nanodots on g-C3N4 nitrogen vacancies for photocatalytic H2 evolution

A Pt2+/Pt0 hybrid nanodot‐modified graphitic carbon nitride (CN) photocatalyst (CNV‐P) was fabricated for the first time using a chemical reduction method, during which nitrogen vacancies in g‐C3N4 assist to stabilize Pt2+ species. It is elucidated that the coexistence of metallic Pt0 and Pt2+ species in the Pt nanodots loaded on g‐C3N4 results in superior photocatalytic H2 evolution performance with very low Pt loadings. The turnover frequencies (TOFs) are 265.91 and 116.38 h−1 for CNV‐P‐0.1 (0.1 wt % Pt) and CNV‐P‐0.5 (0.5 wt % Pt), respectively, which are much higher than for other g‐C3N4‐based photocatalysts with Pt co‐catalyst reported previously. The excellent photocatalytic H2 evolution performance is a result of i) metallic Pt0 facilitating the electron transport and separation and Pt2+ species preventing the undesirable H2 backward reaction, ii) the strong interfacial contact between Pt2+/Pt0 hybrid nanodots and nitrogen vacancies of CNV facilitating the interfacial electron transfer, and iii) the highly dispersed Pt2+/Pt0 hybrid nanodots exposing more active sites for photocatalytic H2 evolution. Our findings are useful for the design of highly active semiconductor‐based photocatalysts with extremely low precious metal content to reduce the catalyst cost while achieving good activity.MOE (Min. of Education, S’pore

Fabricating 3-dimensional human brown adipose microtissues for transplantation studies

Transplanting cell cultured brown adipocytes (BAs) represents a promising approach to prevent and treat obesity (OB) and its associated metabolic disorders, including type 2 diabetes mellitus (T2DM). However, transplanted BAs have a very low survival rate in vivo. The enzymatic dissociation during the harvest of fully differentiated BAs also loses significant cells. There is a critical need for novel methods that can avoid cell death during cell preparation, transplantation, and in vivo. Here, we reported that preparing BAs as injectable microtissues could overcome the problem. We found that 3D culture promoted BA differentiation and UCP-1 expression, and the optimal initial cell aggregate size was 100 μm. The microtissues could be produced at large scales via 3D suspension assisted with a PEG hydrogel and could be cryopreserved. Fabricated microtissues could survive in vivo for long term. They alleviated body weight and fat gain and improved glucose tolerance and insulin sensitivity in high-fat diet (HFD)-induced OB and T2DM mice. Transplanted microtissues impacted multiple organs, secreted protein factors, and influenced the secretion of endogenous adipokines. To our best knowledge, this is the first report on fabricating human BA microtissues and showing their safety and efficacy in T2DM mice. The proposal of transplanting fabricated BA microtissues, the microtissue fabrication method, and the demonstration of efficacy in T2DM mice are all new. Our results show that engineered 3D human BA microtissues have considerable advantages in product scalability, storage, purity, safety, dosage, survival, and efficacy