GLICEROL UNA ALTERNATIVA PARA LA ALIMENTACIÓN DE RUMIANTES

The search for alternatives in animal nutrition has generated interest in using agricultural and industrial byproducts, such as glycerol, byproduct of biodiesel production. Glycerol can be used in ruminant diets due to its high energy content, which allows it to substitute grains in the ration. In this study we revise different pieces of research where glycerol has been supplied in the diet of ovine and bovine livestock, and the results obtained.La búsqueda de alternativas en la nutrición animal ha generado el interés de utilizar subproductos agrícolas e industriales, tales como el glicerol, subproducto de la producción de biodiesel. El glicerol puede ser usado en la alimentación de rumiantes por su alto contenido de energía, lo que permite sustituirlo por granos en la ración. En este trabajo se revisan diferentes investigaciones en las que se ha proporcionado glicerol en la dieta de ovinos y bovinos, y los resultados obtenidos

MICRO Y NANOENCAPSULACIÓN: UNA PERSPECTIVA BIOTECNOLÓGICA EN LA PRODUCCIÓN ANIMAL

The micro and nano encapsulation is defined as the packaging of an active through an encapsulating agent (polymer) acquiring properties and characteristics different from their higher scale for their later liberation, under controlled and determined conditions, which promote an increase in the useful life of the product and ease their manipulation when converting a liquid material to solid forms. The microparticles are spherical particles with sizes from 1 to 250 um. The nanoparticles are polymere submicronic systems (<1 um). In this revision some devices, substances and systems used in animal production and veterinary medicine are detailed, as well as their reach and limitations in different fields of studyLa micro y nanoencapsulación se define como el envasado de un activo mediante un agente encapsulante (polímero) adquiriendo propiedades y características diferentes de su escala mayor para su liberación posterior, bajo condiciones controladas y determinadas, que promueven aumento en la vida útil del producto y facilita su manipulación al convertir un material líquido a formas sólidas. Las micropartículas son partículas esféricas con tamaños desde 1 a 250 um. Las nanopartículas son sistemas poliméricos submicrónicos (<1 um). En esta revisión se detallan algunos dispositivos, sustancias y sistemas utilizados en la producción animal y medicina veterinaria, así como sus alcances y limitaciones en diferentes campos de investigación

Tunability of polycaprolactone hydrophilicity by carboxymethyl cellulose loading

[EN] Carboxymethyl cellulose (CMC) is herein proposed as additive in polycaprolactone (PCL) matrices to obtain composites with tunable hydrophilicity. This composite material can be obtained by compression molding. The thermogravimetric degradation profile, the FTIR spectra, values of the water contact angle (WCA), water and phosphate-buffered saline uptake values, and the results of a cytotoxicity assessment are presented herein. The concentrations of CMC in the groups of samples are 0, 2, 5, 10, and 20%. The WCA on the prewetted state decreases proportionally to the concentration of the additive. These results evidence the possibility of obtaining a PCL-based composite with tunable hydrophilicity. Besides, the biological assessment does not reveal any cytotoxic effects. Therefore, the addition of CMC entails an innovative strategy to control the water affinity of PCL in biomedical applications where such feature is required to improve diffusion of biological medium through, or accelerate degradation by hydrolysis.M.E.A.D. expresses her gratitude for the funding through the PhD Grant Program of ULPGC (Code of the grant: PIFULPGC-2014-ING-ARQU-2). J.A.G.T., A.V.L., and G.V.F. acknowledge the support of the Spanish Ministry of Economy and Competitiveness (MINECO) through the project DPI2015-65401-C3-2-R (including the FEDER financial support).Alemán-Domínguez, M.; Ortega, Z.; Benítez, A.; Vilariño, G.; Gómez-Tejedor, JA.; Vallés Lluch, A. (2017). Tunability of polycaprolactone hydrophilicity by carboxymethyl cellulose loading. Journal of Applied Polymer Science. 135(14):1-6. https://doi.org/10.1002/app.46134S161351

Bioactive and topographically-modified electrospun membranes for the creation of new bone regeneration models

Bone injuries that arise from trauma, cancer treatment, or infection are a major and growing global challenge. An increasingly ageing population plays a key role in this, since a growing number of fractures are due to diseases such as osteoporosis, which place a burden on healthcare systems. Current reparative strategies do not sufficiently consider cell-substrate interactions that are found in healthy tissues; therefore, the need for more complex models is clear. The creation of in vitro defined 3D microenvironments is an emerging topographically-orientated approach that provides opportunities to apply knowledge of cell migration and differentiation mechanisms to the creation of new cell substrates. Moreover, introducing biofunctional agents within in vitro models for bone regeneration has allowed, to a certain degree, the control of cell fate towards osteogenic pathways. In this research, we applied three methods for functionalizing spatially-confined electrospun artificial microenvironments that presented relevant components of the native bone stem cell niche. The biological and osteogenic behaviors of mesenchymal stromal cells (MSCs) were investigated on electrospun micro-fabricated scaffolds functionalized with extracellular matrix (ECM) proteins (collagen I), glycosaminoglycans (heparin), and ceramic-based materials (bioglass). Collagen, heparin, and bioglass (BG) were successfully included in the models without modifying the fibrous structures offered by the polycaprolactone (PCL) scaffolds. Mesenchymal stromal cells (MSCs) were successfully seeded in all the biofunctional scaffolds and they showed an increase in alkaline phosphatase production when exposed to PCL/BG composites. This research demonstrates the feasibility of manufacturing smart and hierarchical artificial microenvironments for studying stem cell behavior and ultimately the potential of incorporating these artificial microenvironments into multifunctional membranes for bone tissue regeneration

Linfangioma quístico mesentérico en el diagnóstico diferencial de la enfermedad inflamatoria pélvica

La enfermedad inflamatoria pélvica (EIP) es un síndrome clínico que incluye todas aquellas alteraciones inflamatorias e infecciosas que comprenden los órganos de la pelvis menor. La infección es habitualmente polimicrobiana y está asociada a mujeres jóvenes con vida sexual activa, nulíparas y usuarias de dispositivo intrauterino (DIU Los abscesos tubo-ováricos son una complicación aguda o crónica, asociada a dicho proceso.). CASO CLÍNICO Presentamos el caso de una mujer de 47 años con dolor abdominal intenso, usuaria de DIU. La primera sospecha diagnóstica fue de EIP con presencia de absceso tuboovárico anexial. Sin embargo la exploración física no orientó en este sentido, por lo que se solicitó TAC abdominopélvico, en el que se informó de la posibilidad de linfangioma quístico mesentérico. Por ello, se contactó con el servicio de Cirugía General que procedió a su resección satisfactoria y sin incidencias. El estudio anatomopatológico confirmo la sospecha radiológica. CONCLUSIÓN Una adecuada exploración física es fundamental en el diagnóstico de la EIP y el absceso tuboovárico. El linfangioma quístico mesentérico puede formar parte del diagnóstico diferencial de las masas anexiales en este contexto. Pelvic inflammatory disease (PID) is a clinical syndrome involving all those inflammatory and infectious alterations affecting the minor pelvis organs. Any infection is often multibacterial and more frequent in sexually active nulliparous young women and intrauterine device (IUD) users. Tuboovarian abscesses can be an acute or chronic complication associated to that process. Our patient was a 47 year old IUD user consulting about intense abdominal pain. The initial clinical suspicion pointed at a case of PID associated to a tubo-ovarian anexial abscess. However, a physical examination did not support this suspicion and an abdominopelvian CAT scan was therefore requested, reporting a possible mesenteric cystic lymphangioma. The General Surgery service was reached for treatment, successfully excising the growth without further incident. Histological analysis confirmed the radiological diagnosis. An adequate physical examination is instrumental while diagnosing PID and tuboovarian abscesses. A mesenteric cystic lymphangioma should be included in a differential diagnosis of anexial growths in such a contex

Efficacy and safety of trabectedin in metastatic uterine leiomyosarcoma: A retrospective multicenter study of the Spanish ovarian cancer research group (GEICO)

Objective: We assessed trabectedin in patients with advanced uterine leiomyosarcoma (uLMS) in real-life clinical practice given according to the marketing authorization. Methods: Thirty-six women from 11 tertiary hospitals across Spain who received trabectedin after anthracycline-containing regimen/s were retrospectively analyzed. The primary endpoint was progression-free survival (PFS). Results: Median PFS and overall survival (OS) since starting trabectedin treatment were 5.4 (95%CI: 3.5–7.3) and 18.5 months (95%CI: 11.5–25.6), respectively. Median OS was significantly higher (P = 0.028) in patients receiving trabectedin in = 2nd line (25.3 months) than in = 3rd (15.1 months) and with ECOG performance status = 1 at trabectedin start (19.8 months) than ECOG 2–3 (6.0 months, P = 0.013). When calculating OS since diagnosis, patients had longer OS with localized disease at diagnosis (87.4 months) vs. locally advanced (30.0 months) or metastatic (44.0 months, P = 0.041); and patients who received adjuvant therapy (87.4 months) compared with those who did not (30.0 months, P = 0.003), especially when receiving radiochemotherapy (106.7 months, P = 0.027). One patient (2.8%) had a complete response (CR) and nine patients (25.0%) achieved a partial response (PR) for an objective response rate of 27.8% with median response duration of 11 months (range: 4–93). Eighteen patients (50.0%) had disease stabilization for a disease control rate (DCR) of 77.8%. More patients receiving trabectedin in 1st-line of advanced disease achieved CR (16.7%) and PR (50.0%) than those in = 2nd line/s (0.0% and 20.0%), whereas the DCR was similar across treatment lines. Reversible neutropenia was the most common grade 3/4 laboratory abnormality (19.4%). Conclusions: Trabectedin confers clinical benefit in patients with recurrent/metastatic uLMS, given after failure to an anthracycline-based regimen being comparable to those reported in clinical trials and with a manageable safety profile

Productive performance and carcass characteristics of New Zealand white and California rabbits and their crosses

Objective: Evaluate the growth performance and carcass characteristics as well as the individual and maternal heterosis effects of New Zealand White (NZB), California (CA) rabbits and their crosses. Design/methodology/approach: 450 offspring rabbits, from 48 females mated to 6 stud rabbits, were evaluated, recording the weight and litter size at birth (TCN) and at weaning (TCD), and weight (PMS), gain (GMD), consumption (CMS), and feed conversion (CONV) during 8 weeks post-weaning. The carcass live weight at slaughter, warm carcass weight with head, carcass yield and parts of the carcass were evaluated. Results: Showed differences (p <0.05) in TCN and TCD with values of 10.47 and 9.03 kits, respectively. When NZB was used as the paternal breed, obtaining an individual heterosis of 5.91% for litter size and 12.44% for weaning weight. In the productive performance and carcass characteristics, the superiority of California breed as paternal breed was evidenced, with average values of 36.05 g in GMD and 2.95 kg in CONV. The average individual weight at the end of the fattening, at 70 days of age, was 2.09 kg and the weight of the carcass 1.16 kg. Post-weaning heterosis for the characteristics evaluated during fattening were positive and moderate. Limitations on study/implications: It is necessary to carry out genetic improvement studies, with different crossing systems and to evaluate results based on the productive and reproductive behavior. Findings/conclusions: in reproductive characteristics, when using NZB as paternal breed, the offspring are superior to those obtained from CA; while in the productive variables the offspring from CA show superior results.Objective: Evaluate the growth performance and carcass characteristics as well as the individual and maternal heterosis effects of New Zealand White (NZB), California (CA) rabbits and their crosses.Design/methodology/approach: 450 offspring rabbits, from 48 females mated to 6 stud rabbits, were evaluated, recording the weight (PNC) and litter size at birth (TCN) and at weaning (TCD), as well as, and weight (PMS), weight gain (GMD), consumption (CMS), and feed conversion (CONV) for during 8 weeks post-weaning. The carcass live weight at slaughter, warm carcass weight with head, carcass yield, and carcass parts of the carcass were evaluated.Results: Showed differences (p <0.05) in TCN and TCD with values of 10.47 and 9.03 kits, respectively. When NZB was used as the paternal breed, obtaining an individual heterosis of 5.91% for litter size and 12.44% for weaning weight. In the productive performance and carcass characteristics, the superiority of the California breed as a paternal breed was evidenced, with average values of 36.05 g in GMD and 2.95 kg in CONV. The averageindividual weight at the end of the fattening, at 70 days of age, was 2.09 kg and the weight of the carcass 1.16 kg. Post-weaning heterosis for the characteristics evaluated during fattening was were positive and moderate.Limitations on study/implications: It is necessary to carry out genetic improvement studies, with different crossing systems and to evaluate results based on the productive and reproductive behavior.Findings/conclusions: in reproductive characteristics, when using NZB as apaternal breed, the offspring are superior to those obtained from CA; while inthe productive variables, the offspring from CA show superior results