Spider Diversity Pattern and Community Composition in the South Eastern Nigeria: An Analysis of Habitat Differences

Spider diversity plays an important role in the balance of nature. Thus, a study on the diversity of spiders was carried out in Awka, Anambra State in the South Eastern Nigeria from January, 2018 to June, 2018. Spiders were collected from three habitats which include: residential areas, farmlands and forest regions in the study area using handpicking and vegetative beating methods. A total of one hundred and twenty-five (125) spiders belonging to ten (10) families and comprising of thirteen (13) species were identified. Ninety-three (93) spiders were collected from residential areas, twenty-one (21) from farmlands and eleven (11) from forest regions. Results of the relative abundance of spider species in different habitat showed that Physocyclus globosus has the highest abundance of species having 71.2% with composition of eighty-nine (89) spiders. Residential areas recorded the highest abundance of spiders having 74.4% and species composition of ninety-three (93) spiders. Forest regions recorded the highest species diversity index (H) of 1.7202. The study revealed that spider diversity is influenced by the type of habitat, vegetation and environmental disturbances.Key words: Diversity; Habitat; Relative Abundance; Spide

A fully validated microbiological assay to evaluate the potency of ceftriaxone sodium

Ceftriaxone (CFTX) sodium is a third-generation, broad-spectrum cephalosporin that is resistant to beta-lactamases. An alternative bioassay for the assessment of the potency of this drug in pharmaceutical formulations has not been previously reported. Thus, this paper reports the development and full validation of a 3 x 3 agar diffusion bioassay using a cylinder-plate method to quantify CFTX sodium in pharmaceutical samples. The strain Staphylococcus aureus ATCC 6538P was used as the test microorganism, and the results of the proposed bioassay displayed high linearity, precision, accuracy, specificity and robustness. All potency results were statistically analyzed using an analysis of variance (ANOVA) and were found to be linear (r=0.99999) in the range of 16-64 µg/mL, accurate (100.5%), and precise [repeatability: relative standard deviation (RSD)=1.4%; intermediate precision: between-day RSD=2.1% and between-analyst RSD=2.5%]. The specificity of the bioassay was determined by evaluating a degraded sample (50 ºC) at 0, 24 and 48 hours as compared against the results from the pharmacopeial liquid chromatography method for CFTX. The results validated the proposed microbiological assay, which allows reliable quantitation of CFTX in pharmaceutical samples. Moreover, it is a useful, simple and low-cost alternative method for monitoring the quality of this medicine