Results of intensive phase tretament of tuberculosis treatment according to CYP2E1 genotype

The polymorphism of the gene of cytochrome P-450 2E1 (CYP2E1) that participate in metabolism of antituberculosis agent isoniazid may influence on effectiveness of tuberculosis (TB) treatment. Aim of research: to detect the peculiarities of pulmonary TB course and outcome after in -patient treatment according to CYP2E1 genotype of the patients with primary TB. Materials and methods: analysis of medical cards from 86 patients with primary pulmonary tuberculosis at the end of hospital treatment in Odessa regional antituberculosal dispensary was conducted in 2012 year with consideration of CYP2E1 genotype. Results: the obtained data has proved that at the beginning of treatment there was no significant difference between TB patients concerning the signs of destruction, infiltration and disintegration according to CYP2E1 polymorphism, however the patients with CD, DD genotype more frequently had sings of dissemination and destruction in pulmonary tissues than the patients with CC genotype. At the end of in-patient treatment the patients with CD, DD genotype more often exhibited signs of infiltration of pulmonary tissues and longer remained smear-positive according to cultural method, than the patients with CC genotype. Despite of CYP2E1 genotype around 25% of tuberculosis patients had multidrug resistant strains of M.tuberculosis in the end of in-patient treatment. It will be important to check in coming researches an influence of CYP2E1 polymorphism in TB-patients on toxicity of antituberculosis therapy

Replication timing in Drosophila and its peculiarities in polytene chromosomes

Drosophila melanogaster is one of the popular model organisms in DNA replication studies. Since the 1960s, DNA replication of polytene chromosomes has been extensively studied by cytological methods. In the recent two decades, the progress in our understanding of DNA replication was associated with new techniques. Use of fluorescent dyes increased the resolution of cytological methods significantly. High-throughput methods allowed analysis of DNA replication on a genome scale, as well as its correlation with chromatin structure and gene activi ty. Precise mapping of the cytological structures of polytene chromosomes to the genome assembly allowed comparison of replication between polytene chromosomes and chromosomes of diploid cells. New features of replication characteristic for D. melanogaster were described for both diploid and polytene chromosomes. Comparison of genomic replication profiles revealed a significant similarity between Drosophila and other well-studi ed eukaryotic species, such as human. Early replication is often confined to intensely transcribed gene-dense regions characterized by multiple replication initiation sites. Features of DNA replication in Drosophila might be explained by a compact genome. The organization of replication in polytene chromosomes has much in common with the organization of replication in chromosomes in diploid cells. The most important feature of replication in polytene chromosomes is its low rate and the dependence of S-phase duration on many factors: external and internal, local and global. The speed of replication forks in D. melanogaster polytene chromosomes is affected by SUUR and Rif1 proteins. It is not known yet how universal the mechanisms associated with these factors are, but their study is very promising

THE STATE OF HYGIENE AND LOCAL IMMUNITY OF THE ORAL CAVITY IN PATIENTS WITH ANOREXIA NERVOSA

Aim: to study the state of local immunity and oral hygiene in patients with GP suffering from AN.Materials and methods: 35 patients with AN were examined at the age of 19-35 years. In the study we used immunological methods, an index assessment of the state of oral hygiene (PMA, API, GI).Results and discussion: in patients of I group poor oral hygiene was established. The amount of lysozyme in the oral fluid in I group was 0.014 ± 0.07 g/l. A decrease SIgA (11SIgA) was established in patients of I group: I, II and control (135.31 ± 23.17, 130.26 ± 24.21 and 300.34 ± 27.38 mg/l, respectively). A tendency to an increase in the level of IgG in the oral fluid in patients of group II compared with I and the control group (5.35 ± 0.36, vs. 4.57 ± 0.04 and 4.98 ± 0.23 g/l, respectively) was established.Conclusions: poor oral hygiene is an objective indicator which reflects low motivation to maintain oral health. The observed immunodeficiency (11SIgA) in patients with GP is a mirror image of changes occurring in the body of patients with AN.Aim: to study the state of local immunity and oral hygiene in patients with GP suffering from AN.Materials and methods: 35 patients with AN were examined at the age of 19-35 years. In the study we used immunological methods, an index assessment of the state of oral hygiene (PMA, API, GI).Results and discussion: in patients of I group poor oral hygiene was established. The amount of lysozyme in the oral fluid in I group was 0.014 ± 0.07 g/l. A decrease SIgA (11SIgA) was established in patients of I group: I, II and control (135.31 ± 23.17, 130.26 ± 24.21 and 300.34 ± 27.38 mg/l, respectively). A tendency to an increase in the level of IgG in the oral fluid in patients of group II compared with I and the control group (5.35 ± 0.36, vs. 4.57 ± 0.04 and 4.98 ± 0.23 g/l, respectively) was established.Conclusions: poor oral hygiene is an objective indicator which reflects low motivation to maintain oral health. The observed immunodeficiency (11SIgA) in patients with GP is a mirror image of changes occurring in the body of patients with AN

Розробка м’ясо-містких напівкопчених ковбас з м’ясом качки мускусної та білого товстолобика

The article is devoted to the substantiation of expediency of combining white carp (Hypophthalmichthys molitrix) with Muscovy duck meat (Cairina moschata), grown in farms of the Sumy region. The recipes of meat-containing minced sausage systems for the production of meat-containing semi-smoked sausages have been developed. A complex of research of nutritional value and functional and technological indices of perfume systems and finished products was carried out. It was shown that the protein content in samples of meat- containing semi-smoked sausages increased by 4.00–5.16% and amounted to 16.75–16.93 g/100 g. The fat content of the developed samples of meat-containing semi-smoked sausages decreased for 41.40–50.92%. The energy value of the prototype samples decreased and ranged from 212 to 241 kcal per 100 g, which is 32.30–40.45% less compared to the analogue formulation. On the basis of the analysis of functional and technological indicators, the possibility of obtaining high functional and technological indicators of the model minced meat-containing semi-smoked sausages. The moisture-bonding ability was in the range of 91.65–94.05%, and wet-binding index – up to 51.69%. The use of duck meat with freshwater fish does not negatively affect the physic-chemical parameters of finished semi-smoked sausages, and allows products with parameters that meet the standard to be obtained. Meat-containing semi-smoked sausages with Muscovy duck meat and white carp have high organoleptic characteristics. The conducted researches show that the developed recipes of meat and fish meat-containing semi-smoked sausages can be recommended for production by enterprises of meat industry.Стаття присвячена обґрунтуванню доцільності комбінування м’яса товстолобика білого (Hypophthalmichthys molitrix) із м’ясом качки мускусної (Cairina moschata), вирощених в господарствах Сумської області. Розроблені рецептури м’ясо-містких фаршевих систем для виготовлення м’ясо-містких напівкопчених ковбас. Проведений комплекс досліджень харчової цінності та функціонально-технологічних показників фаршевих систем та готової продукції. Показано, що вміст білка в зразках м’ясо-містких напівкопчених ковбас збільшився на 4,00–5,16% і становив 16,75–16,93 г/100 г. Вміст жиру в розроблених зразках м’ясо-містких напівкопчених ковбас зменшився на 41,40–50,92%. Енергетична цінність дослідних зразків зменшилась і коливалася від 212 до 241 кКал в 100 г, що на 32,30–40,45% менше порівняно з рецептурою-аналогом. На підставі аналізу функціонально-технологічних показників підтверджено можливість отримання високих функціонально-технологічних показників модельних фаршів м’ясо-містких напівкопчених ковбас: ВЗЗа в межах 91,65–94,05%, ВУЗ – до 51,69%. Використання м’яса качки разом із м’ясом прісноводної риби не впливає негативно на фізико-хімічні показники готових напівкопчених ковбас, і дозволяє отримати вироби із параметрами, що відповідають стандарту. М’ясо-місткі напівкопчені ковбаси із м’ясом качки мускусної та білого товстолобика мають високі органолептичні показники. Проведені дослідження показують, що розроблені рецептури м’ясо-рибних напівкопчених ковбас можуть бути рекомендовані до виробництва підприємствами м’ясної промисловості

Clusterization in the shape isomers of the 56Ni nucleus

The interrelation of the quadrupole deformation and clusterization is investigated in the example of the 56Ni nucleus. The shape isomers, including superdeformed and hyperdeformed states, are obtained as stability regions of the quasidynamical U(3) symmetry based on a Nilsson calculation. Their possible binary clusterizations are investigated by considering both the consequences of the Pauli exclusion principle and the energetic preference

Investigation Of Technological Properties Of Powder Of Eggplants

The aim of the article is to study and to generalize technological properties of eggplant powder, produced by infrared drying at temperatures 50-60 ºС. The results of the conducted complex of studies reflect main technological and consumption properties of the received puree that plays an important role at creating new culinary products.So, for studying technological properties of food eggplant powder, there was considered the complex of base functional-technological properties of powder, produced by infrared drying.For finding optimal conditions of rehydration of eggplant powders, there was studied the influence of such technological factors as: swelling ability; liquid; powder ratio; influence of the solvent temperature on renovation; renovation duration; degree of comminution of powders.Main parameters that influence the renovation ability of dried eggplants are investigated and studied in the article. The results of the studies of technological properties of eggplant powders prove their high rehydration properties. It gives a possibility to use powders at producing different culinary products not only for enriching them with functional ingredients, but also for giving them new technological properties.Based on the obtained results, there was elaborated and presented the new technological scheme of using renewed powders in food compositions

Інформаційна технологія оптимізації мережі моніторингу стану підземних вод з використанням генетичного алгоритму

Groundwater level monitoring networks can provide significant information about water resources in certain area. Cost effective network design is very important for the management. Maintenance cost of the existing monitoring network can be reduced by minimizing the number of the monitoring wells which do not provide useful information or this information can be reproduced. Finding the group of wells, where measurements could be estimated with the lowest error may be a combinatorial problem as there are many possible combinations to check. Genetic algorithm has a great potential for solving such problems because the time spent searching for a satisfactory solution may be less than the time spent for an exhaustive search, but it does not guarantee the optimal result. In this paper we consider the computational scheme for optimizing existing groundwater monitoring network located in Kryvyi Rih, Dnipropetrovsk region, Ukraine, using genetic algorithm based on ordinary kriging technique and inverse distance weighting method. To find the groups of the redundant wells Python application for the network design optimization was developed. The solution was found for the network with 46 monitoring wells. The number of wells to remove was set from 3 to 7. In terminology of the genetic algorithm each possible solution candidate is called a «chromosome». Chromosome is characterized by a set of values known as «genes». In our case, chromosome is a set of monitoring wells. Iteration of the algorithm is called a «generation». Every generation produced the group of possible solution candidates called a «population». Ordinary kriging technique and inverse distance weighting method were used to estimate the measurements for the candidates. Based on actual and estimated values root-mean-square error for each candidate was calculated. Selection was applied to each population to find the candidates with the smallest error and pass them to the next generation. Single-point crossover was used to exchange genes among chromosomes. To maintain diversity within the population procedure known as «mutation» was applied. Mutation occurred for each gene in the chromosome with the 10% probability. In case of mutation, the gene was replaced by a random one not already included in the chromosome. The number of generations was predefined. Based on the results further development of the tool for the groundwater level monitoring network optimization is planned.Рассмотрена вычислительная схема оптимизации сети мониторинга состояния подземных вод с использованием генетического алгоритма на основе кригинга и метода обратных взвешенных расстояний.Розглянуто обчислювальну схему оптимізації мережі моніторингу стану підземних вод з використанням генетичного алгоритму на основі крігінгу та методу обернено зважених відстаней.Розглянуто обчислювальну схему оптимізації мережі моніторингу стану підземних вод з використанням генетичного алгоритму на основі крігінгу та методу обернено зважених відстаней

Clusterization and deformation in heavy nuclei

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Deformation and Clusterization in Atomic Nuclei

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Актуальність викладання освітньої компоненти за вибором студентів «хімія елементів та їх сполук»

Aim. To highlight the relevance of teaching the educational component of the student’s choice “Chemistry of elements and their compounds” (EC CETC). The aim of the EC CETC course is to form a scientific outlook, skills, and abilities in applying chemical laws, study the role of chemical elements in biological processes, and create the basis for studying special ECs, and future professional activities. Materials and methods. Scientific publications, the legislative framework of Ukraine; analytical, systematic, logical teaching methods were used. Results. The relevance of teaching EC CETC has been considered; the peculiarities of the educational process have been shown and analyzed, and the attention has been focused on the importance of information about the biological and chemical properties of chemical elements and their inorganic compounds for future pharmacy specialists. It has been shown that the academic performance in fundamental chemical disciplines generally increased compared to 2020/2921 AY when EC CETC was not studied. Conclusions. After completing the EC CETC course there is an increase in the quality of the academic performance of students in fundamental chemical disciplines, which indicates the importance of this component and the effectiveness of its teaching methods. As a result of studying EC CETC, students expand their knowledge and understanding of the subject area; learn to solve research and/or innovative problems in the field of pharmacy, critically comprehend the results obtained; develop the ability for abstract thinking, analysis, and synthesis. It would be desirable for all students to study both optional ECs – CETC and “Fundamentals of Quantitative Calculations in Pharmacy” since each of them provides a specific basis for the various specialized ECs studied in senior years. It is also necessary to take into account the possibility of testing educational materials on selective ECs, which should later be introduced into the programs of mandatory ECs.Мета статті – висвітлити актуальність викладання освітньої компоненти за вибором студентів «Хімія елементів та їх сполук» (ОК ХЕС). Цілі ОК ХЕС – формування наукового світогляду, умінь і навичок застосування хімічних законів, вивчення ролі хімічних елементів у біологічних процесах, створення підґрунтя для вивчення спеціальних ОК та майбутньої професійної діяльності. Матеріали та методи: наукові публікації, законодавчу базу України досліджено за допомогою аналітичного, систематичного, логічного методів та методів викладання. Результати дослідження. Розглянуто актуальність викладання ОК ХЕС; розкрито та проаналізовано особливості освітнього процесу, закцентовано увагу на важливості відомостей про біологічні та хімічні властивості хімічних елементів та їх неорганічних сполук для майбутніх фахівців фармації. Доведено, що успішність з фундаментальних хімічних дисциплін загалом підвищилась, якщо порівнювати з результатами 20/21 н. р., коли ОК ХЕС не вивчали. Висновки. Після проходження курсу ОК ХЕС спостерігається підвищення якісної успішності студентів з фундаментальних хімічних дисциплін, що свідчить про важливість цієї складової та ефективність методики її викладання. У результаті вивчення ОК ХЕС студенти розширюють знання і розуміння предметної сфери; навчаються розв’язувати задачі дослідницького та/або інноваційного характеру в галузі фармації та критично осмислювати одержані результати; розвивають здатність до абстрактного мислення, аналізу та синтезу. Бажано було б, щоб усі студенти вивчали дві вибіркові ОК – і ОК ХЕС, і ОК «Основи кількісних розрахунків у фармації», бо кожна з них є певною базою для різних ОК, що їх вивчають на старших курсах. Також треба враховувати можливість апробувати навчальні матеріали на вибіркових ОК, які згодом доцільно ввести до програм обов’язкових ОК